NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHK230
Library VH
(Link to library)
Clone ID VHK230
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16571-1
Original site URL
Representative seq. ID VHK230P
(Link to Original site)
Representative DNA sequence 
>VHK230 (VHK230Q) /CSM/VH/VHK2-B/VHK230Q.Seq.d/
AATAAAGTCACTGTTAGAGACGATCATCCATTCACTCCGGTTGATAGTCTTTTAAATCAA
TGTAAAGATTCAAAGCTTTTCTCAAAGTTTGACATGATTATGGGTTATTTCCAAGTGTTA
ATTAATCCAGAACACGCAAGGTACACCGCTTTCATTACACATATTGGTAAATTTGAATAT
ACTAGAATGCCACAAGGTTTAGTCAACTCTCCATCCACATTCGCCAGATTGATGGTCGAA
ATATTTGGAAAAATCAAAAGTTTATTACAATACTTTGATGATCTTTTGGTTCATTCAAAA
CTCGACTACATGGTACATTTCATTGAAATAATTAGAATGCTTCTATATTGTAGAAAGTAC
CTATTATTCATTTCAAGAGAAAAGAGTGAAATGTTAAAGACTGAAGTAGATTTCCTTGGT
TTCCACATTCATAAAGATGGTATATCTCCAAGAGCTGCAAAGGTTAGAGCTATCTCTGAG
TTACCTGAACCAAGAAACGCCAAAGAAGCTGAAGCTGCATTAGGTCTTTTTGGATTCTTC
AGGAGACACATTGAAAATTACGCTGAAAAAACCTATCACCTTTCCAAXXXXXXXXXXTCA
TCAATGAGCAAGTCTACAAAGATGTCACCATTCCAAATCGTTTATGGATTCAATCCACCA
ACTCCCGTTAATCATTTCAATTCATTAACAAAGACTCGTATACCAATGAGCAACATCAAA
AAGATTGTCCGTGATAATATACTAGATGCTCAAATCAATGCTCAAAAGTATTACAACCGA
GGTCGTGGTGATGTTATCTTCGTAGTCGGAGAAAAGGTGATGGTTAAAAGAAAATTCTTC
CAAACTAATCTTTCAAAAGATCTAATCTCTCACAAACTTGAATCCAAGAATTGTGGACCA
TTCATAATCACTGCCGTTCATGGTAATAATGTAACACTCGATTTAGTAGGTTATCCAAAG
AAACACAATGTGTTCAACAAAGATCAAATCGTAAAACTATACGAGGACAGTGAGTGGTTA
AGAGAAGAGATATCGATGCCGGAACCTGAGGAGATGGACGAAGCAAGTTACGAAGTTGAA
AGTATTCTCAATCACGATAAAGTCAAAAAGATGTACCTAGTGAAGTTCAAAGGTTACCCA
GAACCTGAATGGATCAAAGAAGTCGATACAGACTGTGAAGAGTTAGTCAGAGAGTATTGG
AATAACGTGCAGAAGAAACAATTGAACTCAAGAAGAGAAAGAGAGAATGTAACTACAGAA
GCCGTTGAGCCAATTGTGTCACCTCCACTCTCCCAAGAAGTTCAAAGTTCTCAAACATTG
CAACCAATTACTCCAAGACAACAAAACTCCTNCTCAAAACAAAGAAACCAA
sequence update 2002.10.25
Translated Amino Acid sequence 
NKVTVRDDHPFTPVDSLLNQCKDSKLFSKFDMIMGYFQVLINPEHARYTAFITHIGKFEY
TRMPQGLVNSPSTFARLMVEIFGKIKSLLQYFDDLLVHSKLDYMVHFIEIIRMLLYCRKY
LLFISREKSEMLKTEVDFLGFHIHKDGISPRAAKVRAISELPEPRNAKEAEAALGLFGFF
RRHIENYAEKTYHLS---

---SSMSKSTKMSPFQIVYGFNPPTPVNHFNSLTKTRIPMSNIKKIVRDNILDAQINAQK
YYNRGRGDVIFVVGEKVMVKRKFFQTNLSKDLISHKLESKNCGPFIITAVHGNNVTLDLV
GYPKKHNVFNKDQIVKLYEDSEWLREEISMPEPEEMDEASYEVESILNHDKVKKMYLVKF
KGYPEPEWIKEVDTDCEELVREYWNNVQKKQLNSRRERENVTTEAVEPIVSPPLSQEVQS
SQTLQPITPRQQNSXSKQRNQ
Translated Amino Acid sequence (All Frames) 
Frame A:
NKVTVRDDHPFTPVDSLLNQCKDSKLFSKFDMIMGYFQVLINPEHARYTAFITHIGKFEY
TRMPQGLVNSPSTFARLMVEIFGKIKSLLQYFDDLLVHSKLDYMVHFIEIIRMLLYCRKY
LLFISREKSEMLKTEVDFLGFHIHKDGISPRAAKVRAISELPEPRNAKEAEAALGLFGFF
RRHIENYAEKTYHLS---

---SSMSKSTKMSPFQIVYGFNPPTPVNHFNSLTKTRIPMSNIKKIVRDNILDAQINAQK
YYNRGRGDVIFVVGEKVMVKRKFFQTNLSKDLISHKLESKNCGPFIITAVHGNNVTLDLV
GYPKKHNVFNKDQIVKLYEDSEWLREEISMPEPEEMDEASYEVESILNHDKVKKMYLVKF
KGYPEPEWIKEVDTDCEELVREYWNNVQKKQLNSRRERENVTTEAVEPIVSPPLSQEVQS
SQTLQPITPRQQNSXSKQRNQ

Frame B:
ikslletiihslrlivf*invkiqsfsqslt*lwviskc*liqntqgtplslhilvnlni
lechkv*stlhphspd*wskylekskvyyntlmifwfiqnsttwyislk*lecfyivest
yysfqekrvkc*rlk*islvstfikmvylqelqrlelslsylnqetpkklklh*vfldss
gdtlkitlkkpitfp---

---hq*aslqrchhsksfmdsihqlpliisih*qrlvyq*atskrlsviiy*mlksmlks
ittevvvmlss*sekr*wlkenssklifqki*sltnlnprivdhs*slpfmvim*hsi**
viqrntmcstkiks*nytrtvsg*ekryrcrnlrrwtkqvtklkvfsitikskrct**ss
kvtqnlngskksiqtvks*sesigitcrrnn*tqeekerm*lqkplsqlchlhspkkfkv
lkhcnqllqdnktpxqnket

Frame C:
*shc*rrssihsg**sfksm*rfkaflkv*hdyglfpsvn*srtrkvhrfhytyw*i*iy
*natrfsqlsihirqidgrniwknqkfitil**sfgsfktrlhgtfh*nn*nasil*kvp
iihfkrke*nvkd*srfpwfphs*rwyisksckg*syl*vt*tkkrqrs*scirsfwilq
eth*klr*knlspfq---

---ineqvykdvtipnrlwiqstnsr*sfqfinkdsytneqhqkdcp**ytrcsnqcskv
lqprsw*cylrsrrkgdg*kkilpn*sfkrsnlsqt*iqelwtihnhcrsw**cntrfsr
lsketqcvqqrsnrktirgq*vvkrrdidagt*gdgrsklrs*kysqsr*sqkdvpsevq
rlprt*mdqrsryrl*rvsqrvle*raeetielkkrkrecnyrsr*ancvtstlprsskf
sniatnyskttklllktkkp
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK230 (VHK230Q) /CSM/VH/VHK2-B/VHK230Q.Seq.d/                       2652   0.0
SFJ625 (SFJ625Q) /CSM/SF/SFJ6-B/SFJ625Q.Seq.d/                       1499   0.0
AHB822 (AHB822Q) /CSM/AH/AHB8-A/AHB822Q.Seq.d/                       1497   0.0
AHM541 (AHM541Q) /CSM/AH/AHM5-B/AHM541Q.Seq.d/                       1495   0.0
AHC690 (AHC690Q) /CSM/AH/AHC6-D/AHC690Q.Seq.d/                       1495   0.0
AHF244 (AHF244Q) /CSM/AH/AHF2-B/AHF244Q.Seq.d/                       1493   0.0
AHA263 (AHA263Q) /CSM/AH/AHA2-C/AHA263Q.Seq.d/                       1491   0.0
SHL821 (SHL821Q) /CSM/SH/SHL8-A/SHL821Q.Seq.d/                       1481   0.0
AHN739 (AHN739Q) /CSM/AH/AHN7-B/AHN739Q.Seq.d/                       1481   0.0
AHD676 (AHD676Q) /CSM/AH/AHD6-D/AHD676Q.Seq.d/                       1469   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442932) Dictyostelium discoideum cDNA clone:ddv51k08, 3' ...  1528   0.0    1
(BJ348729) Dictyostelium discoideum cDNA clone:dda34l06, 3' ...  1497   0.0    1
(BJ355260) Dictyostelium discoideum cDNA clone:dda56a11, 3' ...  1495   0.0    1
(BJ349526) Dictyostelium discoideum cDNA clone:dda36c24, 3' ...  1495   0.0    1
(BJ350887) Dictyostelium discoideum cDNA clone:dda41g12, 3' ...  1493   0.0    1
(BJ347937) Dictyostelium discoideum cDNA clone:dda31m16, 3' ...  1491   0.0    1
(BJ408839) Dictyostelium discoideum cDNA clone:dds47j06, 3' ...  1473   0.0    2
(BJ350128) Dictyostelium discoideum cDNA clone:dda38g20, 3' ...  1469   0.0    1
(AF049230) Dictyostelium discoideum LTR-retrotransposon Skip...  1449   0.0    1
(AF017040) Dictyostelium discoideum LTR-retrotransposon Skip...  1443   0.0    1
dna update 2008.11.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF049230_3(AF049230|pid:none) Dictyostelium discoideum LTR-retro...   478   e-133
AY198186_2(AY198186|pid:none) Saccharomyces paradoxus Ty3-like r...   136   2e-30
AF541963_5(AF541963|pid:none) Glycine max cv Williams 82 BAC clo...   135   3e-30
AF364550_2(AF364550|pid:none) Drosophila melanogaster transposon...   134   1e-29
AJ966994_1(AJ966994|pid:none) Lotus corniculatus var. japonicus ...   132   4e-29
AC007730_14(AC007730|pid:none) Arabidopsis thaliana chromosome 2...   130   1e-28
CR380953_301(CR380953|pid:none) Candida glabrata strain CBS138 c...   128   4e-28
AY673967_1(AY673967|pid:none) Candida glabrata transposon LTR-re...   128   4e-28
(Q99315) RecName: Full=Transposon Ty3-G Gag-Pol polyprotein; Alt...   127   7e-28
(Q7LHG5) RecName: Full=Transposon Ty3-I Gag-Pol polyprotein; Alt...   127   7e-28
protein update 2009. 3.13
PSORT 

 psg: 0.71  gvh: 0.32  alm: 0.41  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: mitochondrial

>> prediction for VHK230 is cyt
5' end seq. ID VHK230F
5' end seq. 
>VHK230F.Seq
AATAAAGTCACTGTTAGAGACGATCATCCATTCACTCCGGTTGATAGTCTTTTAAATCAA
TGTAAAGATTCAAAGCTTTTCTCAAAGTTTGACATGATTATGGGTTATTTCCAAGTGTTA
ATTAATCCAGAACACGCAAGGTACACCGCTTTCATTACACATATTGGTAAATTTGAATAT
ACTAGAATGCCACAAGGTTTAGTCAACTCTCCATCCACATTCGCCAGATTGATGGTCGAA
ATATTTGGAAAAATCAAAAGTTTATTACAATACTTTGATGATCTTTTGGTTCATTCAAAA
CTCGACTACATGGTACATTTCATTGAAATAATTAGAATGCTTCTATATTGTAGAAAGTAC
CTATTATTCATTTCAAGAGAAAAGAGTGAAATGTTAAAGACTGAAGTAGATTTCCTTGGT
TTCCACATTCATAAAGATGGTATATCTCCAAGAGCTGCAAAGGTTAGAGCTATCTCTGAG
TTACCTGAACCAAGAAACGCCAAAGAAGCTGAAGCTGCATTAGGTCTTTTTGGATTCTTC
AGGAGACACATTGAAAATTACGCTGAAAAAACCTATCACCTTTCCAANNNNNNNNNN
Length of 5' end seq. 597
3' end seq. ID VHK230Z
3' end seq. 
>VHK230Z.Seq
NNNNNNNNNNTCATCAATGAGCAAGTCTACAAAGATGTCACCATTCCAAATCGTTTATGG
ATTCAATCCACCAACTCCCGTTAATCATTTCAATTCATTAACAAAGACTCGTATACCAAT
GAGCAACATCAAAAAGATTGTCCGTGATAATATACTAGATGCTCAAATCAATGCTCAAAA
GTATTACAACCGAGGTCGTGGTGATGTTATCTTCGTAGTCGGAGAAAAGGTGATGGTTAA
AAGAAAATTCTTCCAAACTAATCTTTCAAAAGATCTAATCTCTCACAAACTTGAATCCAA
GAATTGTGGACCATTCATAATCACTGCCGTTCATGGTAATAATGTAACACTCGATTTAGT
AGGTTATCCAAAGAAACACAATGTGTTCAACAAAGATCAAATCGTAAAACTATACGAGGA
CAGTGAGTGGTTAAGAGAAGAGATATCGATGCCGGAACCTGAGGAGATGGACGAAGCAAG
TTACGAAGTTGAAAGTATTCTCAATCACGATAAAGTCAAAAAGATGTACCTAGTGAAGTT
CAAAGGTTACCCAGAACCTGAATGGATCAAAGAAGTCGATACAGACTGTGAAGAGTTAGT
CAGAGAGTATTGGAATAACGTGCAGAAGAAACAATTGAACTCAAGAAGAGAAAGAGAGAA
TGTAACTACAGAAGCCGTTGAGCCAATTGTGTCACCTCCACTCTCCCAAGAAGTTCAAAG
TTCTCAAACATTGCAACCAATTACTCCAAGACAACAAAACTCCTNCTCAAAACAAAGAAA
CCAA
Length of 3' end seq. 784
Connected seq. ID VHK230P
Connected seq. 
>VHK230P.Seq
AATAAAGTCACTGTTAGAGACGATCATCCATTCACTCCGGTTGATAGTCTTTTAAATCAA
TGTAAAGATTCAAAGCTTTTCTCAAAGTTTGACATGATTATGGGTTATTTCCAAGTGTTA
ATTAATCCAGAACACGCAAGGTACACCGCTTTCATTACACATATTGGTAAATTTGAATAT
ACTAGAATGCCACAAGGTTTAGTCAACTCTCCATCCACATTCGCCAGATTGATGGTCGAA
ATATTTGGAAAAATCAAAAGTTTATTACAATACTTTGATGATCTTTTGGTTCATTCAAAA
CTCGACTACATGGTACATTTCATTGAAATAATTAGAATGCTTCTATATTGTAGAAAGTAC
CTATTATTCATTTCAAGAGAAAAGAGTGAAATGTTAAAGACTGAAGTAGATTTCCTTGGT
TTCCACATTCATAAAGATGGTATATCTCCAAGAGCTGCAAAGGTTAGAGCTATCTCTGAG
TTACCTGAACCAAGAAACGCCAAAGAAGCTGAAGCTGCATTAGGTCTTTTTGGATTCTTC
AGGAGACACATTGAAAATTACGCTGAAAAAACCTATCACCTTTCCAA----------TCA
TCAATGAGCAAGTCTACAAAGATGTCACCATTCCAAATCGTTTATGGATTCAATCCACCA
ACTCCCGTTAATCATTTCAATTCATTAACAAAGACTCGTATACCAATGAGCAACATCAAA
AAGATTGTCCGTGATAATATACTAGATGCTCAAATCAATGCTCAAAAGTATTACAACCGA
GGTCGTGGTGATGTTATCTTCGTAGTCGGAGAAAAGGTGATGGTTAAAAGAAAATTCTTC
CAAACTAATCTTTCAAAAGATCTAATCTCTCACAAACTTGAATCCAAGAATTGTGGACCA
TTCATAATCACTGCCGTTCATGGTAATAATGTAACACTCGATTTAGTAGGTTATCCAAAG
AAACACAATGTGTTCAACAAAGATCAAATCGTAAAACTATACGAGGACAGTGAGTGGTTA
AGAGAAGAGATATCGATGCCGGAACCTGAGGAGATGGACGAAGCAAGTTACGAAGTTGAA
AGTATTCTCAATCACGATAAAGTCAAAAAGATGTACCTAGTGAAGTTCAAAGGTTACCCA
GAACCTGAATGGATCAAAGAAGTCGATACAGACTGTGAAGAGTTAGTCAGAGAGTATTGG
AATAACGTGCAGAAGAAACAATTGAACTCAAGAAGAGAAAGAGAGAATGTAACTACAGAA
GCCGTTGAGCCAATTGTGTCACCTCCACTCTCCCAAGAAGTTCAAAGTTCTCAAACATTG
CAACCAATTACTCCAAGACAACAAAACTCCTNCTCAAAACAAAGAAACCAA
Length of connected seq. 1361
Full length Seq ID -
Full length Seq. -
Length of full length seq. -