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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHK226
Library VH
(Link to library)
Clone ID VHK226
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U15510-1
Original site URL
Representative seq. ID VHK226P
(Link to Original site)
Representative DNA sequence 
>VHK226 (VHK226Q) /CSM/VH/VHK2-B/VHK226Q.Seq.d/
AACCAAATCAAGAAAAACTAATAATATATTTAAGATGTCTGAAAATTCATCACCAAAATT
AGAATCAACATCAGCAGCAGCAGCAAGTACAAAAAAACCATTCCCAGAATGGATTAAACC
TAAAGGCAAAGAAACTGAATTATTAATTAATAATAGTTTAACAGGTGGTAAAGTACCATT
CGTTTTCAATGAAAGTGGTAAAGGTAGATCATTAACATGGTATGCTTGTGGTCCAACAGT
TTATGATGCATCACATATGGGTCATGCTAGAACTTATATTAGTTTTGATATCATTAGACG
TATTATGAAAAATTATCTTGGTTTCAATATCCAATATGTTATGAACATTACCGATATTGA
TGATAAAATCATTATTCGTGCAAATGAAAATGGTATTTCACATAGTGATCTTTCAAAAAA
ATGGGAGACTGCTTTCTTTGAAGATATGAAATTATTAAATGTATTACCACCAGATGCATT
AACAAGAGTTACAGAATATGTACCACAAATTGTTGAATATGTTGAAAAGATTATTTCAAA
TGGATTTGCATATGAATCAAATGGGATCAGTTTATTTTGATACAGTAGCATTTTCAAAAG
CACATGATTATGGTAAATTAGXXXXXXXXXXTTTATCAGATCTCGTCAATAAAACAAATG
TTTACATTAGATCATGTGCTGAACAAAAGACAAATCCAAGATTAAATTTAATTAGTGCAA
TCGCAGAGTATATCACTTACATTTTCTCTGTTTTCGGTTTAACCGAATCTTCAACCGCCT
CTTCAATGATTGGTTTCGGTTCCGCTGGTAAAGGTAATATCGAAGAGGAGATGACTCCAA
TCTTAAATGCTCTCACTCAATTCCGTTCAGAGGTACGTGCTTCTGCAATCGCAAAAGATA
CTACCTCAATCCTTAAAACTTGTGATAACCTTCGTGATGAGGTTTTACCATTATTGGGCG
TAAAGATTGATGATAAATCCGCAACAACTGCAATGTGGAAATTTGAAGATAAAGAAACCC
TCAAAAAAGAAATTGAACAAAAGAAAGAAATTGAAAAGAAGAAACAAGCTGACAAGGAAG
AAAAAGAAAAAAAATTAAAAGAGAAATTTGAAAAATCAAAAATTCCACCACAACAACTCT
TTATCAATGAAACTGATAAATACTCAAAATTCAATGAATTGGGTATGCCAACTCATGATA
AGGAAGGTGTTGAAATTACAAAATCACAATTAAAAAAATTACAAAAAGAATATGATAATC
AAACTAAAGAACATAATAATTATTTGAAATCATTATCAACTTCAACATCATCACCAACTT
TAACTTCAACTCAATCACCACAATAAAATTATTTTAAAATAAAATAAAATAAAATAAAAT
AAAATAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
pnqekliiylrclkihhqn*nqhqqqqqvqknhsqnglnlkakklny*liiv*qvvkyhs
fsmkvvkvdh*hgmlvvqqfmmhhiwvmlelilvlisldvl*kiilvsisnml*tlpilm
ikslfvqmkmvfhivifqkngrllslki*ny*myyhqmh*QELQNMYHKLLNMLKRLFQM
DLHMNQMGSVYFDTVAFSKAHDYGKL---

---LSDLVNKTNVYIRSCAEQKTNPRLNLISAIAEYITYIFSVFGLTESSTASSMIGFGS
AGKGNIEEEMTPILNALTQFRSEVRASAIAKDTTSILKTCDNLRDEVLPLLGVKIDDKSA
TTAMWKFEDKETLKKEIEQKKEIEKKKQADKEEKEKKLKEKFEKSKIPPQQLFINETDKY
SKFNELGMPTHDKEGVEITKSQLKKLQKEYDNQTKEHNNYLKSLSTSTSSPTLTSTQSPQ
*nyfkik*nkik*nk
Translated Amino Acid sequence (All Frames) 
Frame A:
nqikkn**yi*dv*kfitkirinisssskykktiprmd*t*rqrn*iin***fnrw*sti
rfq*kw*r*iinmvclwsnsl*citygsc*nly*f*yh*tyyeklswfqypicyehyry*
**nhysck*kwyft**sfkkmgdcfl*ryeiikcittrcinksyricttnc*ic*kdyfk
wici*ikwdqfiliq*hfqkhmimvn*---

---firsrq*nkclh*imc*tkdkskikfn*cnrrvyhlhflcfrfnrifnrlfndwfrf
rw*r*yrrgddsnlkcshsipfrgtcfcnrkryylnp*nl**ps**gftiigrkd***ir
nncnvei*r*rnpqkrn*tkern*keets*qgrkrkkikrei*kiknsttttlyq*n**i
lkiq*igyans**grc*nykitikkitkri**sn*rt**lfeiiinfniitnfnfnsitt
iklf*nkik*nkik*

Frame B:
tksrktnnifkmsensspklestsaaaastkkpfpewikpkgketellinnsltggkvpf
vfnesgkgrsltwyacgptvydashmghartyisfdiirrimknylgfniqyvmnitdid
dkiiiranengishsdlskkwetaffedmkllnvlppdaltrvteyvpqiveyvekiisn
gfayesngislf*yssifkst*lw*i---

---LSDLVNKTNVYIRSCAEQKTNPRLNLISAIAEYITYIFSVFGLTESSTASSMIGFGS
AGKGNIEEEMTPILNALTQFRSEVRASAIAKDTTSILKTCDNLRDEVLPLLGVKIDDKSA
TTAMWKFEDKETLKKEIEQKKEIEKKKQADKEEKEKKLKEKFEKSKIPPQQLFINETDKY
SKFNELGMPTHDKEGVEITKSQLKKLQKEYDNQTKEHNNYLKSLSTSTSSPTLTSTQSPQ
*nyfkik*nkik*nk

Frame C:
pnqekliiylrclkihhqn*nqhqqqqqvqknhsqnglnlkakklny*liiv*qvvkyhs
fsmkvvkvdh*hgmlvvqqfmmhhiwvmlelilvlisldvl*kiilvsisnml*tlpilm
ikslfvqmkmvfhivifqkngrllslki*ny*myyhqmh*QELQNMYHKLLNMLKRLFQM
DLHMNQMGSVYFDTVAFSKAHDYGKL---

---yqissikqmftldhvlnkrqiqd*i*lvqsqsisltfslfsv*pnlqpplq*lvsvp
lvkviskrr*lqs*mlslnsvqryvllqsqkilpqslklvitfvmrfyhywa*rlminpq
qlqcgnlkikkpskkklnkrkklkrrnkltrkkkkkn*krnlknqkfhhnnslsmklint
qnsmnwvcqlmirkvlklqnhn*knykknmiiklkniiii*nhyqlqhhhql*lqlnhhn
kiilk*nkik*nkik
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK226 (VHK226Q) /CSM/VH/VHK2-B/VHK226Q.Seq.d/                       1834   0.0
AFH281 (AFH281Q) /CSM/AF/AFH2-D/AFH281Q.Seq.d/                       1132   0.0
VHC506 (VHC506Q) /CSM/VH/VHC5-A/VHC506Q.Seq.d/                       1120   0.0
AFO752 (AFO752Q) /CSM/AF/AFO7-C/AFO752Q.Seq.d/                       1118   0.0
AFN666 (AFN666Q) /CSM/AF/AFN6-C/AFN666Q.Seq.d/                       1118   0.0
AFJ714 (AFJ714Q) /CSM/AF/AFJ7-A/AFJ714Q.Seq.d/                       1118   0.0
AFG246 (AFG246Q) /CSM/AF/AFG2-B/AFG246Q.Seq.d/                       1118   0.0
SFD396 (SFD396Q) /CSM/SF/SFD3-D/SFD396Q.Seq.d/                       1116   0.0
AFO629 (AFO629Q) /CSM/AF/AFO6-B/AFO629Q.Seq.d/                       1114   0.0
AFJ344 (AFJ344Q) /CSM/AF/AFJ3-B/AFJ344Q.Seq.d/                       1110   0.0
own update 2003. 1. 1
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442887) Dictyostelium discoideum cDNA clone:ddv51c08, 3' ...   726   0.0    3
(BJ348700) Dictyostelium discoideum cDNA clone:dda34f01, 3' ...   726   0.0    3
(BJ438337) Dictyostelium discoideum cDNA clone:ddv37j14, 3' ...   698   0.0    3
(BJ424058) Dictyostelium discoideum cDNA clone:ddv51c08, 5' ...   628   0.0    3
(BJ419911) Dictyostelium discoideum cDNA clone:ddv37j14, 5' ...   628   0.0    4
(BJ419444) Dictyostelium discoideum cDNA clone:ddv36k01, 5' ...   628   0.0    4
(BJ388108) Dictyostelium discoideum cDNA clone:dds7p23, 5' e...   628   0.0    4
(BJ331145) Dictyostelium discoideum cDNA clone:dda34f01, 5' ...   628   0.0    4
(BJ330128) Dictyostelium discoideum cDNA clone:dda27p10, 5' ...   628   0.0    4
(BJ329052) Dictyostelium discoideum cDNA clone:dda30h13, 5' ...   628   0.0    4
dna update 2008.11.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE014185_147(AE014185|pid:none) Plasmodium falciparum 3D7 chromo...   181   6e-44
BX538350_42(BX538350|pid:none) Cryptosporidium parvum chromosome...   174   5e-42
AL590448_49(AL590448|pid:none) chromosome VIII of strain GB-M1 o...   159   3e-37
BC166536_1(BC166536|pid:none) Rattus norvegicus similar to hypot...   154   6e-36
(Q8BYM8) RecName: Full=Probable cysteinyl-tRNA synthetase, mitoc...   152   3e-35
BC007220_1(BC007220|pid:none) Homo sapiens cysteinyl-tRNA synthe...   152   3e-35
(Q9HA77) RecName: Full=Probable cysteinyl-tRNA synthetase, mitoc...   152   3e-35
AK021302_1(AK021302|pid:none) Mus musculus 13 days embryo stomac...   152   3e-35
AK009937_1(AK009937|pid:none) Mus musculus adult male tongue cDN...   152   3e-35
AK041962_1(AK041962|pid:none) Mus musculus 3 days neonate thymus...   152   3e-35
protein update 2009. 7.15
PSORT 

 psg: 0.64  gvh: 0.30  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.21  mip: 0.04
 nuc: 0.06  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: cytoskeletal
	  4.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: plasma membrane

>> prediction for VHK226 is cyt
5' end seq. ID VHK226F
5' end seq. 
>VHK226F.Seq
AACCAAATCAAGAAAAACTAATAATATATTTAAGATGTCTGAAAATTCATCACCAAAATT
AGAATCAACATCAGCAGCAGCAGCAAGTACAAAAAAACCATTCCCAGAATGGATTAAACC
TAAAGGCAAAGAAACTGAATTATTAATTAATAATAGTTTAACAGGTGGTAAAGTACCATT
CGTTTTCAATGAAAGTGGTAAAGGTAGATCATTAACATGGTATGCTTGTGGTCCAACAGT
TTATGATGCATCACATATGGGTCATGCTAGAACTTATATTAGTTTTGATATCATTAGACG
TATTATGAAAAATTATCTTGGTTTCAATATCCAATATGTTATGAACATTACCGATATTGA
TGATAAAATCATTATTCGTGCAAATGAAAATGGTATTTCACATAGTGATCTTTCAAAAAA
ATGGGAGACTGCTTTCTTTGAAGATATGAAATTATTAAATGTATTACCACCAGATGCATT
AACAAGAGTTACAGAATATGTACCACAAATTGTTGAATATGTTGAAAAGATTATTTCAAA
TGGATTTGCATATGAATCAAATGGGATCAGTTTATTTTGATACAGTAGCATTTTCAAAAG
CACATGATTATGGTAAATTAGNNNNNNNNNN
Length of 5' end seq. 631
3' end seq. ID VHK226Z
3' end seq. 
>VHK226Z.Seq
NNNNNNNNNNTTTATCAGATCTCGTCAATAAAACAAATGTTTACATTAGATCATGTGCTG
AACAAAAGACAAATCCAAGATTAAATTTAATTAGTGCAATCGCAGAGTATATCACTTACA
TTTTCTCTGTTTTCGGTTTAACCGAATCTTCAACCGCCTCTTCAATGATTGGTTTCGGTT
CCGCTGGTAAAGGTAATATCGAAGAGGAGATGACTCCAATCTTAAATGCTCTCACTCAAT
TCCGTTCAGAGGTACGTGCTTCTGCAATCGCAAAAGATACTACCTCAATCCTTAAAACTT
GTGATAACCTTCGTGATGAGGTTTTACCATTATTGGGCGTAAAGATTGATGATAAATCCG
CAACAACTGCAATGTGGAAATTTGAAGATAAAGAAACCCTCAAAAAAGAAATTGAACAAA
AGAAAGAAATTGAAAAGAAGAAACAAGCTGACAAGGAAGAAAAAGAAAAAAAATTAAAAG
AGAAATTTGAAAAATCAAAAATTCCACCACAACAACTCTTTATCAATGAAACTGATAAAT
ACTCAAAATTCAATGAATTGGGTATGCCAACTCATGATAAGGAAGGTGTTGAAATTACAA
AATCACAATTAAAAAAATTACAAAAAGAATATGATAATCAAACTAAAGAACATAATAATT
ATTTGAAATCATTATCAACTTCAACATCATCACCAACTTTAACTTCAACTCAATCACCAC
AATAAAATTATTTTAAAATAAAATAAAATAAAATAAAATAAAATAAAA
Length of 3' end seq. 768
Connected seq. ID VHK226P
Connected seq. 
>VHK226P.Seq
AACCAAATCAAGAAAAACTAATAATATATTTAAGATGTCTGAAAATTCATCACCAAAATT
AGAATCAACATCAGCAGCAGCAGCAAGTACAAAAAAACCATTCCCAGAATGGATTAAACC
TAAAGGCAAAGAAACTGAATTATTAATTAATAATAGTTTAACAGGTGGTAAAGTACCATT
CGTTTTCAATGAAAGTGGTAAAGGTAGATCATTAACATGGTATGCTTGTGGTCCAACAGT
TTATGATGCATCACATATGGGTCATGCTAGAACTTATATTAGTTTTGATATCATTAGACG
TATTATGAAAAATTATCTTGGTTTCAATATCCAATATGTTATGAACATTACCGATATTGA
TGATAAAATCATTATTCGTGCAAATGAAAATGGTATTTCACATAGTGATCTTTCAAAAAA
ATGGGAGACTGCTTTCTTTGAAGATATGAAATTATTAAATGTATTACCACCAGATGCATT
AACAAGAGTTACAGAATATGTACCACAAATTGTTGAATATGTTGAAAAGATTATTTCAAA
TGGATTTGCATATGAATCAAATGGGATCAGTTTATTTTGATACAGTAGCATTTTCAAAAG
CACATGATTATGGTAAATTAG----------TTTATCAGATCTCGTCAATAAAACAAATG
TTTACATTAGATCATGTGCTGAACAAAAGACAAATCCAAGATTAAATTTAATTAGTGCAA
TCGCAGAGTATATCACTTACATTTTCTCTGTTTTCGGTTTAACCGAATCTTCAACCGCCT
CTTCAATGATTGGTTTCGGTTCCGCTGGTAAAGGTAATATCGAAGAGGAGATGACTCCAA
TCTTAAATGCTCTCACTCAATTCCGTTCAGAGGTACGTGCTTCTGCAATCGCAAAAGATA
CTACCTCAATCCTTAAAACTTGTGATAACCTTCGTGATGAGGTTTTACCATTATTGGGCG
TAAAGATTGATGATAAATCCGCAACAACTGCAATGTGGAAATTTGAAGATAAAGAAACCC
TCAAAAAAGAAATTGAACAAAAGAAAGAAATTGAAAAGAAGAAACAAGCTGACAAGGAAG
AAAAAGAAAAAAAATTAAAAGAGAAATTTGAAAAATCAAAAATTCCACCACAACAACTCT
TTATCAATGAAACTGATAAATACTCAAAATTCAATGAATTGGGTATGCCAACTCATGATA
AGGAAGGTGTTGAAATTACAAAATCACAATTAAAAAAATTACAAAAAGAATATGATAATC
AAACTAAAGAACATAATAATTATTTGAAATCATTATCAACTTCAACATCATCACCAACTT
TAACTTCAACTCAATCACCACAATAAAATTATTTTAAAATAAAATAAAATAAAATAAAAT
AAAATAAAA
Length of connected seq. 1379
Full length Seq ID -
Full length Seq. -
Length of full length seq. -