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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHK186
Library VH
(Link to library)
Clone ID VHK186
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U13711-1|Contig-U15744-1
Original site URL
Representative seq. ID VHK186P
(Link to Original site)
Representative DNA sequence 
>VHK186 (VHK186Q) /CSM/VH/VHK1-D/VHK186Q.Seq.d/
ATNAGAGATTGAAGATCGTTTAATTAAAATTGCAGAAGCAATGTTTCCTGATATTCATTT
ATCAAATATTTATCAACAGCAACAGCAACAGCAGCAACAACAACAACAATCCTCTCCAAG
TAAATCATCATCAACTTCACCAATTATAAAATCATTAAATCTATCAACAGTAAGTGAATT
AGGTGAATCTTCAAATCAAACTCCAAAACAAAATATAACAACAACTACAACTACAACTCA
CAGTGATCATAAGAGACAACTTTCAACTGATAGTGGTAGTAGCTATCGTAATAAAAGTCA
TGATACAATTAGTCATTCAACAAGTGTTGCATCAGATTTATTAGATATTGATAATACATT
AACAGTTGCAACAACACCAAGAAATAGATCAAAAACAAATGATGATAATATTATTAATAC
AAGTAATGGTAGAATTATAACAAATTCAATTAATAATAGTAATAGTAATAACGAACAACC
ATTATCACCAAATTCATTACTTCAACATTCACAATCACAACAACAATTAGAAAATGTTGG
ATTAAGTGCATTATTAGATGCATTACCAAATTCACCAATTGTTTCATCAACTGCAGCTCC
TTCTACAACATCTACCAATAAAAAAGTGATGTATACATCGATTGTXXXXXXXXXXTCTGA
TGGTACTATTAAAGTTTGGTCCTCAACTTGTCAATCAGTTCATACCATTGAAAATGCACA
CTCTAGTAGAATCTTTACATTGGAATTGGTTGGTGATTTCGTTTGGTCTGGAAGTTGGGA
TACAACGATCAAAATTTGGTCAACAAAAGATTATCATTTGGTTAGTGAAAATTCTGGTAA
ACATAAAGATGCAATTAGTAGTTTTGTTTACATATCAAATGATCAACCATTACAAACCAA
TGAACGTCCAATTCAAAAACAAGTTTGGTCTGGTAGTTGGGATTCAAGTATTTGTGTTTG
GGCTTTACCAAATGATACAAACTCTAGATCAAATACAATCTTTAGTTCTGATTCTCAATT
TAATTTAAATGGTGGTAGTAGTAATTCAATAACAAATAGTAATAGTAATAGTAATAATAA
TTTAAATGGTAATTTAAATAATAGTAATAATAGTATTAATAATAATTATAATAATAATAA
TAACAATAATAATAATAATAATAATTATTATTATAGTAATAATGTAAATAGTCCAAATCA
AGCTTCTCAAAGTGCTGGTCATCTTGGTACAATTCATGAACAAACTTCACCAAATTCTGC
AACACCATTATCATCTACACCTCCTGGTAGTAAAGGTTTAATGCAAAGAAGAACCG
sequence update 2002.10.25
Translated Amino Acid sequence 
XEIEDRLIKIAEAMFPDIHLSNIYQQQQQQQQQQQQSSPSKSSSTSPIIKSLNLSTVSEL
GESSNQTPKQNITTTTTTTHSDHKRQLSTDSGSSYRNKSHDTISHSTSVASDLLDIDNTL
TVATTPRNRSKTNDDNIINTSNGRIITNSINNSNSNNEQPLSPNSLLQHSQSQQQLENVG
LSALLDALPNSPIVSSTAAPSTTSTNKKVMYTSI---

---SDGTIKVWSSTCQSVHTIENAHSSRIFTLELVGDFVWSGSWDTTIKIWSTKDYHLVS
ENSGKHKDAISSFVYISNDQPLQTNERPIQKQVWSGSWDSSICVWALPNDTNSRSNTIFS
SDSQFNLNGGSSNSITNSNSNSNNNLNGNLNNSNNSINNNYNNNNNNNNNNNNYYYSNNV
NSPNQASQSAGHLGTIHEQTSPNSATPLSSTPPGSKGLMQRRT
Translated Amino Acid sequence (All Frames) 
Frame A:
xrd*rsfn*ncrsnvs*ysfikylstatataattttilsk*iiinftnykiiksinsk*i
r*ifksnsktkynnnynynsq*s*ettfn**w**ls**ks*yn*sfnkccirfiry**yi
nscnntkk*iknk***yy*yk*w*nynkfn*******rttiitkfitstftitttirkcw
ikciircitkftncfincssfyniyq*ksdvyidc---

---SDGTIKVWSSTCQSVHTIENAHSSRIFTLELVGDFVWSGSWDTTIKIWSTKDYHLVS
ENSGKHKDAISSFVYISNDQPLQTNERPIQKQVWSGSWDSSICVWALPNDTNSRSNTIFS
SDSQFNLNGGSSNSITNSNSNSNNNLNGNLNNSNNSINNNYNNNNNNNNNNNNYYYSNNV
NSPNQASQSAGHLGTIHEQTSPNSATPLSSTPPGSKGLMQRRT

Frame B:
XEIEDRLIKIAEAMFPDIHLSNIYQQQQQQQQQQQQSSPSKSSSTSPIIKSLNLSTVSEL
GESSNQTPKQNITTTTTTTHSDHKRQLSTDSGSSYRNKSHDTISHSTSVASDLLDIDNTL
TVATTPRNRSKTNDDNIINTSNGRIITNSINNSNSNNEQPLSPNSLLQHSQSQQQLENVG
LSALLDALPNSPIVSSTAAPSTTSTNKKVMYTSI---

---lmvllkfgpqlvnqfiplkmhtlveslhwnwlvisfglevgiqrskfgqqkiiiwlv
kilvnikmqlvvlftyqminhykpmnvqfknkfglvvgiqvfvfglyqmiqtldqiqslv
lilnli*mvvvviq*qiviviviii*mvi*iiviivliiiiiiiitiiiiiiiiiivim*
ivqikllkvlvilvqfmnklhqilqhhyhlhllvvkv*ckeep

Frame C:
xrlkiv*lklqkqcflifiyqifinsnsnssnnnnnplqvnhhqlhql*nh*iyqq*vn*
vnlqiklqnki*qqlqlqltviirdnfqlivvvaivikvmiqlviqqvlhqiy*iliih*
qlqqhqeidqkqmmiilliqvmvel*qiqliivivitnnhyhqihyfnihnhnnn*kmld
*vhy*mhyqihqlfhqlqlllqhlpikk*cihrl---

---*wyy*slvlnlsissyh*kctl**nlyigigw*frlvwklgyndqnlvnkrlsfg**
kfw*t*rcn**fclhik*stitnq*tsnsktslvw*lgfkylclgftk*ykl*ikynl*f
*fsi*fkww***fnnk********fkw*fk*****y***l*****q******lll***ck
*skssfskcwsswyns*tnftkfcntiiiytsw**rfnakkn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHK186 (VHK186Q) /CSM/VH/VHK1-D/VHK186Q.Seq.d/                       1754   0.0
SHD196 (SHD196Q) /CSM/SH/SHD1-D/SHD196Q.Seq.d/                        672   0.0
SLA465 (SLA465Q) /CSM/SL/SLA4-C/SLA465Q.Seq.d/                        153   8e-36
VHF566 (VHF566Q) /CSM/VH/VHF5-C/VHF566Q.Seq.d/                        131   3e-29
SSL372 (SSL372Q) /CSM/SS/SSL3-C/SSL372Q.Seq.d/                         50   9e-05
VSA877 (VSA877Q) /CSM/VS/VSA8-D/VSA877Q.Seq.d/                         48   3e-04
SFH110 (SFH110Q) /CSM/SF/SFH1-A/SFH110Q.Seq.d/                         48   3e-04
AFK440 (AFK440Q) /CSM/AF/AFK4-B/AFK440Q.Seq.d/                         48   3e-04
AFI767 (AFI767Q) /CSM/AF/AFI7-C/AFI767Q.Seq.d/                         48   3e-04
SFE156 (SFE156Q) /CSM/SF/SFE1-C/SFE156Q.Seq.d/                         46   0.001
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AY170918) Dictyostelium discoideum pats1 gene, partial cds.      716   0.0    4
(BJ424261) Dictyostelium discoideum cDNA clone:ddv51k21, 5' ...   587   0.0    3
(BJ443092) Dictyostelium discoideum cDNA clone:ddv51k21, 3' ...   716   0.0    2
(BJ405215) Dictyostelium discoideum cDNA clone:dds30o23, 3' ...   656   0.0    2
(AU060157) Dictyostelium discoideum slug cDNA, clone SLA465.      153   5e-59  2
(BJ440061) Dictyostelium discoideum cDNA clone:ddv42c17, 3' ...   131   7e-26  1
(AU261803) Dictyostelium discoideum vegetative cDNA clone:VS...    48   0.003  2
(BJ326935) Dictyostelium discoideum cDNA clone:dda18f17, 5' ...    48   0.004  2
(BJ391086) Dictyostelium discoideum cDNA clone:dds15c03, 5' ...    48   0.004  2
(AC117072) Dictyostelium discoideum chromosome 2 map 3879572...    42   0.005  9
dna update 2008.11.21
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55E58) RecName: Full=Probable serine/threonine-protein kinase ...   258   3e-67
AY170918_1(AY170918|pid:none) Dictyostelium discoideum pats1 gen...   258   3e-67
CP001324_582(CP001324|pid:none) Micromonas sp. RCC299 chromosome...    59   5e-07
CP001291_2528(CP001291|pid:none) Cyanothece sp. PCC 7424, comple...    53   3e-05
AF1890(AF1890) WD-repeat protein [imported] - Nostoc sp. (strain...    53   3e-05
CR954209_40(CR954209|pid:none) Ostreococcus tauri strain OTTH059...    52   4e-05
AC007727_7(AC007727|pid:none) Arabidopsis thaliana chromosome 1 ...    51   8e-05
CP000589_46(CP000589|pid:none) Ostreococcus lucimarinus CCE9901 ...    51   8e-05
CP000117_2178(CP000117|pid:none) Anabaena variabilis ATCC 29413,...    51   1e-04
AC1842(AC1842) WD-40 repeat protein [imported] - Nostoc sp. (str...    51   1e-04
protein update 2009. 7.15
PSORT 

 psg: 0.75  gvh: 0.40  alm: 0.43  top: 0.53  tms: 0.00  mit: 0.10  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHK186 is cyt
5' end seq. ID VHK186F
5' end seq. 
>VHK186F.Seq
ATNAGAGATTGAAGATCGTTTAATTAAAATTGCAGAAGCAATGTTTCCTGATATTCATTT
ATCAAATATTTATCAACAGCAACAGCAACAGCAGCAACAACAACAACAATCCTCTCCAAG
TAAATCATCATCAACTTCACCAATTATAAAATCATTAAATCTATCAACAGTAAGTGAATT
AGGTGAATCTTCAAATCAAACTCCAAAACAAAATATAACAACAACTACAACTACAACTCA
CAGTGATCATAAGAGACAACTTTCAACTGATAGTGGTAGTAGCTATCGTAATAAAAGTCA
TGATACAATTAGTCATTCAACAAGTGTTGCATCAGATTTATTAGATATTGATAATACATT
AACAGTTGCAACAACACCAAGAAATAGATCAAAAACAAATGATGATAATATTATTAATAC
AAGTAATGGTAGAATTATAACAAATTCAATTAATAATAGTAATAGTAATAACGAACAACC
ATTATCACCAAATTCATTACTTCAACATTCACAATCACAACAACAATTAGAAAATGTTGG
ATTAAGTGCATTATTAGATGCATTACCAAATTCACCAATTGTTTCATCAACTGCAGCTCC
TTCTACAACATCTACCAATAAAAAAGTGATGTATACATCGATTGTNNNNNNNNNN
Length of 5' end seq. 655
3' end seq. ID VHK186Z
3' end seq. 
>VHK186Z.Seq
NNNNNNNNNNTCTGATGGTACTATTAAAGTTTGGTCCTCAACTTGTCAATCAGTTCATAC
CATTGAAAATGCACACTCTAGTAGAATCTTTACATTGGAATTGGTTGGTGATTTCGTTTG
GTCTGGAAGTTGGGATACAACGATCAAAATTTGGTCAACAAAAGATTATCATTTGGTTAG
TGAAAATTCTGGTAAACATAAAGATGCAATTAGTAGTTTTGTTTACATATCAAATGATCA
ACCATTACAAACCAATGAACGTCCAATTCAAAAACAAGTTTGGTCTGGTAGTTGGGATTC
AAGTATTTGTGTTTGGGCTTTACCAAATGATACAAACTCTAGATCAAATACAATCTTTAG
TTCTGATTCTCAATTTAATTTAAATGGTGGTAGTAGTAATTCAATAACAAATAGTAATAG
TAATAGTAATAATAATTTAAATGGTAATTTAAATAATAGTAATAATAGTATTAATAATAA
TTATAATAATAATAATAACAATAATAATAATAATAATAATTATTATTATAGTAATAATGT
AAATAGTCCAAATCAAGCTTCTCAAAGTGCTGGTCATCTTGGTACAATTCATGAACAAAC
TTCACCAAATTCTGCAACACCATTATCATCTACACCTCCTGGTAGTAAAGGTTTAATGCA
AAGAAGAACCG
Length of 3' end seq. 671
Connected seq. ID VHK186P
Connected seq. 
>VHK186P.Seq
ATNAGAGATTGAAGATCGTTTAATTAAAATTGCAGAAGCAATGTTTCCTGATATTCATTT
ATCAAATATTTATCAACAGCAACAGCAACAGCAGCAACAACAACAACAATCCTCTCCAAG
TAAATCATCATCAACTTCACCAATTATAAAATCATTAAATCTATCAACAGTAAGTGAATT
AGGTGAATCTTCAAATCAAACTCCAAAACAAAATATAACAACAACTACAACTACAACTCA
CAGTGATCATAAGAGACAACTTTCAACTGATAGTGGTAGTAGCTATCGTAATAAAAGTCA
TGATACAATTAGTCATTCAACAAGTGTTGCATCAGATTTATTAGATATTGATAATACATT
AACAGTTGCAACAACACCAAGAAATAGATCAAAAACAAATGATGATAATATTATTAATAC
AAGTAATGGTAGAATTATAACAAATTCAATTAATAATAGTAATAGTAATAACGAACAACC
ATTATCACCAAATTCATTACTTCAACATTCACAATCACAACAACAATTAGAAAATGTTGG
ATTAAGTGCATTATTAGATGCATTACCAAATTCACCAATTGTTTCATCAACTGCAGCTCC
TTCTACAACATCTACCAATAAAAAAGTGATGTATACATCGATTGT----------TCTGA
TGGTACTATTAAAGTTTGGTCCTCAACTTGTCAATCAGTTCATACCATTGAAAATGCACA
CTCTAGTAGAATCTTTACATTGGAATTGGTTGGTGATTTCGTTTGGTCTGGAAGTTGGGA
TACAACGATCAAAATTTGGTCAACAAAAGATTATCATTTGGTTAGTGAAAATTCTGGTAA
ACATAAAGATGCAATTAGTAGTTTTGTTTACATATCAAATGATCAACCATTACAAACCAA
TGAACGTCCAATTCAAAAACAAGTTTGGTCTGGTAGTTGGGATTCAAGTATTTGTGTTTG
GGCTTTACCAAATGATACAAACTCTAGATCAAATACAATCTTTAGTTCTGATTCTCAATT
TAATTTAAATGGTGGTAGTAGTAATTCAATAACAAATAGTAATAGTAATAGTAATAATAA
TTTAAATGGTAATTTAAATAATAGTAATAATAGTATTAATAATAATTATAATAATAATAA
TAACAATAATAATAATAATAATAATTATTATTATAGTAATAATGTAAATAGTCCAAATCA
AGCTTCTCAAAGTGCTGGTCATCTTGGTACAATTCATGAACAAACTTCACCAAATTCTGC
AACACCATTATCATCTACACCTCCTGGTAGTAAAGGTTTAATGCAAAGAAGAACCG
Length of connected seq. 1306
Full length Seq ID -
Full length Seq. -
Length of full length seq. -