>AFO372 (AFO372Q) /CSM/AF/AFO3-C/AFO372Q.Seq.d/
XXXXXXXXXXTGATTGGGAATTACTTCCATCAAANCAAATCAAAGATCCAAAACAACCCA
AACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCG
GTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGA
ATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTG
AATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAA
TTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTA
TCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCG
ACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCG
AAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCNG

---DWELLPSXQIKDPKQPKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWN
EEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAI
GFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKA

Frame A:
---DWELLPSXQIKDPKQPKPVDWVDVKEIDDPEDVKPAGHDDIPASIVDPEAVKPEDWN
EEDDGEWEAPTIANPEYKGEWKAKKIPNPEYKGEWVHPLIDNPEYAEDNELYLFNDLGAI
GFELWQVKSGSIFNNMIVTDSVEEAKDFSEKTFVANQEAEKKMFDDLEAAKAEERKKA


Frame B:
---ignyfhqxkskiqnnpnqsiglt*rksmtqkmlnqpvmtisqlqsliqklsnqrigm
kkttvngkpqpspiqntkvngkqrksqiqntkvngyth*litqntlktmnstslmisvls
vsnygklnlvpslti*ssptlskklkislkrpllptkkpkrkclttskppklkrerkx


Frame C:
---lgitsikxnqrskttqtsrlg*rkgnr*prrc*tsrs*rypsfnr*srscqtrgle*
rrrr*mgspnhrqsriqr*meskenpksriqr*mgtpin**prir*rq*tlpl**srcyr
frimas*iwfhl*qydrhrlcrrs*rfl*kdlccqprsrkenv*rprsrqs*rekes

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO372 (AFO372Q) /CSM/AF/AFO3-C/AFO372Q.Seq.d/                       1035   0.0
VFM129 (VFM129Q) /CSM/VF/VFM1-B/VFM129Q.Seq.d/                       1027   0.0
VFL130 (VFL130Q) /CSM/VF/VFL1-B/VFL130Q.Seq.d/                       1027   0.0
VFK631 (VFK631Q) /CSM/VF/VFK6-B/VFK631Q.Seq.d/                       1027   0.0
VFI619 (VFI619Q) /CSM/VF/VFI6-A/VFI619Q.Seq.d/                       1027   0.0
VFG242 (VFG242Q) /CSM/VF/VFG2-B/VFG242Q.Seq.d/                       1027   0.0
VFE283 (VFE283Q) /CSM/VF/VFE2-D/VFE283Q.Seq.d/                       1027   0.0
VFE122 (VFE122Q) /CSM/VF/VFE1-A/VFE122Q.Seq.d/                       1027   0.0
SFK591 (SFK591Q) /CSM/SF/SFK5-D/SFK591Q.Seq.d/                       1027   0.0
SFJ301 (SFJ301Q) /CSM/SF/SFJ3-A/SFJ301Q.Seq.d/                       1027   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

U36937|U36937.1 Dictyostelium discoideum calreticulin mRNA, complete cds.  1027  0.0  1
BH160872|BH160872.1 ENTRI18TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  2e-16  3
AZ543757|AZ543757.1 ENTGD24TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    80  3e-11  1
BE459798|BE459798.1 EST415090 tomato developing/immature green fruit Lycopersicon esculentum cDNA clone cLEM8G13, mRNA sequence.    74  2e-09  1
AI771812|AI771812.1 EST252912 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED38E1, mRNA sequence.    74  2e-09  1
BE435540|BE435540.1 EST406618 tomato breaker fruit, TIGR Lycopersicon esculentum cDNA clone cLEG27F24, mRNA sequence.    74  2e-09  1
AW932476|AW932476.1 EST358319 tomato fruit mature green, TAMU Lycopersicon esculentum cDNA clone cLEF48O8 5', mRNA sequence.    74  2e-09  1
AW033447|AW033447.1 EST277018 tomato callus, TAMU Lycopersicon esculentum cDNA clone cLEC28B21 similar to calreticulin, putative, mRNA sequence.    74  2e-09  1
AI489195|AI489195.1 EST247534 tomato ovary, TAMU Lycopersicon esculentum cDNA clone cLED17D1, mRNA sequence.    74  2e-09  1
AW649196|AW649196.1 EST327650 tomato germinating seedlings, TAMU Lycopersicon esculentum cDNA clone cLEI7M10 5', mRNA sequence.    74  2e-09  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U36937_1(U36937|pid:none) Dictyostelium discoideum calreticulin ...   312   4e-84
(Q9STD3) RecName: Full=Calreticulin; Flags: Precursor;  &AJ00076...   195   7e-49
(Q9XF98) RecName: Full=Calreticulin; Flags: Precursor;  &AF13473...   194   9e-49
AF283816_1(AF283816|pid:none) Pinus taeda calreticulin mRNA, com...   192   3e-48
AK230218_1(AK230218|pid:none) Arabidopsis thaliana mRNA for hypo...   191   9e-48
AM445600_2(AM445600|pid:none) Vitis vinifera contig VV78X277184....   190   2e-47
AB196794_1(AB196794|pid:none) Glycine max Gm crt-1 mRNA for calr...   188   6e-47
EF085019_1(EF085019|pid:none) Picea sitchensis clone WS0297_I16 ...   187   1e-46
AC149080_5(AC149080|pid:none) Medicago truncatula clone mth2-75j...   187   2e-46
AK104328_1(AK104328|pid:none) Oryza sativa Japonica Group cDNA c...   186   2e-46

 psg: 0.75  gvh: 0.25  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.08  mip: 0.00
 nuc: 0.06  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 40.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for AFO372 is nuc

>AFO372Z.Seq
----------TGATTGGGAATTACTTCCATCAAANCAAATCAAAGATCCAAAACAACCCA
AACCAGTCGATTGGGTTGACGTAAAGGAAATCGATGACCCAGAAGATGTTAAACCAGCCG
GTCATGACGATATCCCAGCTTCAATCGTTGATCCAGAAGCTGTCAAACCAGAGGATTGGA
ATGAAGAAGACGACGGTGAATGGGAAGCCCCAACCATCGCCAATCCAGAATACAAAGGTG
AATGGAAAGCAAAGAAAATCCCAAATCCAGAATACAAAGGTGAATGGGTACACCCATTAA
TTGATAACCCAGAATACGCTGAAGACAATGAACTCTACCTCTTTAATGATCTCGGTGCTA
TCGGTTTCGAATTATGGCAAGTTAAATCTGGTTCCATCTTTAACAATATGATCGTCACCG
ACTCTGTCGAAGAAGCTAAAGATTTCTCTGAAAAGACCTTTGTTGCCAACCAAGAAGCCG
AAAAGAAAATGTTTGACGACCTCGAAGCCGCCAAAGCTGAAGAGAGAAAGAAAGCNG