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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHJ867
Library VH
(Link to library)
Clone ID VHJ867
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15717-1
Original site URL
Representative seq. ID VHJ867P
(Link to Original site)
Representative DNA sequence 
>VHJ867 (VHJ867Q) /CSM/VH/VHJ8-C/VHJ867Q.Seq.d/
CCTCTTGAACCAAACAAGAAACTCAACTACGAAGGTATTGATGCTAAATTAAAACAATTC
AGACTCCATCACAACAAACCACTCACCCTTGCTGAAAAGATCATTTATGGTCATTTAGAA
GATCCATCAACTAAAGTTGAAAGAGGTATCACTTATCTTAAATTACATCCAGATCGTGTT
GCTATGCAAGATGCCACCGCACAAATGGCTGTATTACAATTTATGTCAGCTGGTTTACCA
GAAACTGCAGTACCAACAACAATTCATTGTGATCATTTAATTGAAGCATATAAAGGTGGT
GAAAAAGATTTAGAAGTTGCAAAAGATATCAATAAAGAAGTTTACGATTTCCTTTCAACC
AGTGCTAAGAAATTTGGTATGGGTTTCTGGAAGCCAGGTTCAGGTATTATCCATCAAATC
GTTTTAGAAAATTATGCATTCCCAGGTGGTTTAATGATTGGTACTGATAGTCATACACCA
AATGCTGGTGGTTTAGGTATGGTCGCAGTTGGTGTTGGTGGTGCTGATGCCGTCGATGTT
ATGGCAGGTATCCCATGGGAATTGAAAGCACCAXXXXXXXXXXGTGATGAACTCCCCATC
ACGTGGTTTTGATGCCGGTGAAAACACTTACCAACCACCATCACCAAATGGTCAAAACAT
TAACGTTATCGTTGATTCAGAAAGCAGCAGATTACAATTACTCCAACCATTTGCCCCATG
GGATAAGAAAGATTTAGTCGATATGCAAGTTCTCATCAAGGTTCAAGGTAAATGTACCAC
CGATCACATCTCTATGGCTGGTCCATGGTTGAAATACAGAGGTCATTTGGATAACATTTC
CAACAATATGTTAATTGGTGCTATCAACTCTGAAAACGGTAAAGCCAACGCTGTTCTCAA
TCAATTCACTGGTGAAATCGGTCCAGTCCCAACCGTCGCTCGTGACTACAAGAAACGTGG
TGTCAATTGGATCGTCGTTGGTGATGAAAACTATGGTGAAGGTTCATCTCGTGAACATGC
TGCCCTCGAACCACGTCATCTCGGTGGTAAAGCTATCCTCGTTAAATCATTTGCTCGTAT
CCACGAAACCAATCTCAAGAAACAAGGTATCTTACCATTAACCTTTGCTAACCCATCTGA
TTACGATAAAATCTCTGGTGACGATCGTACTTCAATCATTGGTCTCAAAGATCTTGCCCC
AGGTAAACAACTCACTCTCATTGTTAAATCTGCTAAACAAGGTTCTGAATTGAAATTAAA
GCTAATCACACCATGAATGCTGGTCAAATCGAAGGTTCAAAGCTGGTTCCGCTTTAAATT
ATATTAAATCAGAAAAAGCCAAAAAAAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
PLEPNKKLNYEGIDAKLKQFRLHHNKPLTLAEKIIYGHLEDPSTKVERGITYLKLHPDRV
AMQDATAQMAVLQFMSAGLPETAVPTTIHCDHLIEAYKGGEKDLEVAKDINKEVYDFLST
SAKKFGMGFWKPGSGIIHQIVLENYAFPGGLMIGTDSHTPNAGGLGMVAVGVGGADAVDV
MAGIPWELKAP---

---VMNSPSRGFDAGENTYQPPSPNGQNINVIVDSESSRLQLLQPFAPWDKKDLVDMQVL
IKVQGKCTTDHISMAGPWLKYRGHLDNISNNMLIGAINSENGKANAVLNQFTGEIGPVPT
VARDYKKRGVNWIVVGDENYGEGSSREHAALEPRHLGGKAILVKSFARIHETNLKKQGIL
PLTFANPSDYDKISGDDRTSIIGLKDLAPGKQLTLIVKSAKQGSELKLKLITP*mlvksk
vqswfrfkly*irksqkk
Translated Amino Acid sequence (All Frames) 
Frame A:
PLEPNKKLNYEGIDAKLKQFRLHHNKPLTLAEKIIYGHLEDPSTKVERGITYLKLHPDRV
AMQDATAQMAVLQFMSAGLPETAVPTTIHCDHLIEAYKGGEKDLEVAKDINKEVYDFLST
SAKKFGMGFWKPGSGIIHQIVLENYAFPGGLMIGTDSHTPNAGGLGMVAVGVGGADAVDV
MAGIPWELKAP---

---VMNSPSRGFDAGENTYQPPSPNGQNINVIVDSESSRLQLLQPFAPWDKKDLVDMQVL
IKVQGKCTTDHISMAGPWLKYRGHLDNISNNMLIGAINSENGKANAVLNQFTGEIGPVPT
VARDYKKRGVNWIVVGDENYGEGSSREHAALEPRHLGGKAILVKSFARIHETNLKKQGIL
PLTFANPSDYDKISGDDRTSIIGLKDLAPGKQLTLIVKSAKQGSELKLKLITP*mlvksk
vqswfrfkly*irksqkk

Frame B:
llnqtrnsttkvlmln*nnsdsittnhspllkrsfmvi*kihqlklkevslilnyiqivl
lckmpphkwlyynlcqlvyqklqyqqqfivii*lkhikvvkki*klqkisikkftisfqp
vlrnlvwvsgsqvqvlsiksf*kimhsqvv**lvlivihqmlvv*vwsqlvlvvlmpsml
wqvshgn*kh---

---**tphhvvlmpvktltnhhhqmvktltlsliqkaadynysnhlphgirki*sickfs
srfkvnvppitslwlvhg*nteviwitfptic*lvlstlktvkptlfsinslvksvqsqp
slvttrnvvsigsslvmktmvkvhlvnmlpsnhvisvvklsslnhllvstkpisrnkvsy
h*pllthlitikslvtivlqslvskilpqvnnslsllnllnkvln*n*s*shhecwsnrr
fkagsalnyiksekakkn

Frame C:
s*tkqetqlrry*c*iktiqtpsqqtthpc*kdhlwsfrrsin*s*kryhls*itsrscc
yarchrtngcitiyvswftrncstnnnsl*sfn*si*rw*krfrsckryq*rslrfpfnq
c*eiwygflearfryypsnrfrklciprwfndwy**sytkcwwfrygrswcwwc*crrcy
grypmgiest---

---delpitwf*cr*khlpttitkwskh*ryr*frkqqititpticpmg*erfsryassh
qgsr*myhrshlygwsmveiqrsfg*hfqqyvnwcyql*kr*sqrcsqsihw*nrsspnr
rs*lqetwcqldrrw**klw*rfis*tccprttssrw*sypr*iicsyprnqsqetrylt
inlc*pi*lr*nlw*rsyfnhwsqrscpr*tthshc*ic*trf*ieikanhtmnagqieg
sklvpl*iilnqkkpkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHJ867 (VHJ867Q) /CSM/VH/VHJ8-C/VHJ867Q.Seq.d/                       2597   0.0
VHJ814 (VHJ814Q) /CSM/VH/VHJ8-A/VHJ814Q.Seq.d/                       1449   0.0
VHJ504 (VHJ504Q) /CSM/VH/VHJ5-A/VHJ504Q.Seq.d/                       1380   0.0
VHO133 (VHO133Q) /CSM/VH/VHO1-B/VHO133Q.Seq.d/                       1358   0.0
VHN217 (VHN217Q) /CSM/VH/VHN2-A/VHN217Q.Seq.d/                       1356   0.0
VHC367 (VHC367Q) /CSM/VH/VHC3-C/VHC367Q.Seq.d/                       1338   0.0
VHF201 (VHF201Q) /CSM/VH/VHF2-A/VHF201Q.Seq.d/                       1328   0.0
VHD386 (VHD386Q) /CSM/VH/VHD3-D/VHD386Q.Seq.d/                       1298   0.0
CHQ607 (CHQ607Q) /CSM/CH/CHQ6-A/CHQ607Q.Seq.d/                       1287   0.0
SLA476 (SLA476Q) /CSM/SL/SLA4-D/SLA476Q.Seq.d/                       1160   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442653) Dictyostelium discoideum cDNA clone:ddv50f18, 3' ...  1499   0.0    1
(BJ442525) Dictyostelium discoideum cDNA clone:ddv50l04, 3' ...  1294   0.0    3
(BJ445359) Dictyostelium discoideum cDNA clone:ddv59a09, 3' ...  1225   0.0    3
(BJ442499) Dictyostelium discoideum cDNA clone:ddv50g01, 3' ...  1217   0.0    2
(BJ437847) Dictyostelium discoideum cDNA clone:ddv35f17, 3' ...  1205   0.0    3
(BJ385501) Dictyostelium discoideum cDNA clone:ddc55m02, 3' ...  1172   0.0    3
(BJ438430) Dictyostelium discoideum cDNA clone:ddv37l21, 3' ...  1168   0.0    3
(BJ439599) Dictyostelium discoideum cDNA clone:ddv41a02, 3' ...  1166   0.0    2
(BJ423836) Dictyostelium discoideum cDNA clone:ddv50f18, 5' ...  1136   0.0    1
(BJ444634) Dictyostelium discoideum cDNA clone:ddv57a06, 3' ...  1076   0.0    4
dna update 2008.11.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB105050_1(AB105050|pid:none) Antheraea yamamai mRNA for hypothe...   318   2e-85
BC058049_1(BC058049|pid:none) Danio rerio aconitase 2, mitochond...   311   5e-83
AE014134_3462(AE014134|pid:none) Drosophila melanogaster chromos...   310   6e-83
AE014134_3463(AE014134|pid:none) Drosophila melanogaster chromos...   310   6e-83
(P49609) RecName: Full=Aconitate hydratase, mitochondrial;      ...   310   8e-83
S44831(S44831)F54H12.1 protein - Caenorhabditis elegans               306   3e-82
L25599_8(L25599|pid:none) Caenorhabditis elegans cosmid F54H12, ...   306   3e-82
L25599_7(L25599|pid:none) Caenorhabditis elegans cosmid F54H12, ...   306   3e-82
AJ245736_1(AJ245736|pid:none) Daphnia pulex mRNA for aconitase.       306   6e-82
Z49931_1(Z49931|pid:none) B.taurus mRNA for mitochondrial aconit...   305   2e-81
protein update 2009. 7.15
PSORT 

 psg: 0.52  gvh: 0.31  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 60.0 %: cytoplasmic
	 24.0 %: nuclear
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: peroxisomal

>> prediction for VHJ867 is cyt
5' end seq. ID VHJ867F
5' end seq. 
>VHJ867F.Seq
CCTCTTGAACCAAACAAGAAACTCAACTACGAAGGTATTGATGCTAAATTAAAACAATTC
AGACTCCATCACAACAAACCACTCACCCTTGCTGAAAAGATCATTTATGGTCATTTAGAA
GATCCATCAACTAAAGTTGAAAGAGGTATCACTTATCTTAAATTACATCCAGATCGTGTT
GCTATGCAAGATGCCACCGCACAAATGGCTGTATTACAATTTATGTCAGCTGGTTTACCA
GAAACTGCAGTACCAACAACAATTCATTGTGATCATTTAATTGAAGCATATAAAGGTGGT
GAAAAAGATTTAGAAGTTGCAAAAGATATCAATAAAGAAGTTTACGATTTCCTTTCAACC
AGTGCTAAGAAATTTGGTATGGGTTTCTGGAAGCCAGGTTCAGGTATTATCCATCAAATC
GTTTTAGAAAATTATGCATTCCCAGGTGGTTTAATGATTGGTACTGATAGTCATACACCA
AATGCTGGTGGTTTAGGTATGGTCGCAGTTGGTGTTGGTGGTGCTGATGCCGTCGATGTT
ATGGCAGGTATCCCATGGGAATTGAAAGCACCANNNNNNNNNN
Length of 5' end seq. 583
3' end seq. ID VHJ867Z
3' end seq. 
>VHJ867Z.Seq
NNNNNNNNNNGTGATGAACTCCCCATCACGTGGTTTTGATGCCGGTGAAAACACTTACCA
ACCACCATCACCAAATGGTCAAAACATTAACGTTATCGTTGATTCAGAAAGCAGCAGATT
ACAATTACTCCAACCATTTGCCCCATGGGATAAGAAAGATTTAGTCGATATGCAAGTTCT
CATCAAGGTTCAAGGTAAATGTACCACCGATCACATCTCTATGGCTGGTCCATGGTTGAA
ATACAGAGGTCATTTGGATAACATTTCCAACAATATGTTAATTGGTGCTATCAACTCTGA
AAACGGTAAAGCCAACGCTGTTCTCAATCAATTCACTGGTGAAATCGGTCCAGTCCCAAC
CGTCGCTCGTGACTACAAGAAACGTGGTGTCAATTGGATCGTCGTTGGTGATGAAAACTA
TGGTGAAGGTTCATCTCGTGAACATGCTGCCCTCGAACCACGTCATCTCGGTGGTAAAGC
TATCCTCGTTAAATCATTTGCTCGTATCCACGAAACCAATCTCAAGAAACAAGGTATCTT
ACCATTAACCTTTGCTAACCCATCTGATTACGATAAAATCTCTGGTGACGATCGTACTTC
AATCATTGGTCTCAAAGATCTTGCCCCAGGTAAACAACTCACTCTCATTGTTAAATCTGC
TAAACAAGGTTCTGAATTGAAATTAAAGCTAATCACACCATGAATGCTGGTCAAATCGAA
GGTTCAAAGCTGGTTCCGCTTTAAATTATATTAAATCAGAAAAAGCCAAAAAAAAT
Length of 3' end seq. 776
Connected seq. ID VHJ867P
Connected seq. 
>VHJ867P.Seq
CCTCTTGAACCAAACAAGAAACTCAACTACGAAGGTATTGATGCTAAATTAAAACAATTC
AGACTCCATCACAACAAACCACTCACCCTTGCTGAAAAGATCATTTATGGTCATTTAGAA
GATCCATCAACTAAAGTTGAAAGAGGTATCACTTATCTTAAATTACATCCAGATCGTGTT
GCTATGCAAGATGCCACCGCACAAATGGCTGTATTACAATTTATGTCAGCTGGTTTACCA
GAAACTGCAGTACCAACAACAATTCATTGTGATCATTTAATTGAAGCATATAAAGGTGGT
GAAAAAGATTTAGAAGTTGCAAAAGATATCAATAAAGAAGTTTACGATTTCCTTTCAACC
AGTGCTAAGAAATTTGGTATGGGTTTCTGGAAGCCAGGTTCAGGTATTATCCATCAAATC
GTTTTAGAAAATTATGCATTCCCAGGTGGTTTAATGATTGGTACTGATAGTCATACACCA
AATGCTGGTGGTTTAGGTATGGTCGCAGTTGGTGTTGGTGGTGCTGATGCCGTCGATGTT
ATGGCAGGTATCCCATGGGAATTGAAAGCACCA----------GTGATGAACTCCCCATC
ACGTGGTTTTGATGCCGGTGAAAACACTTACCAACCACCATCACCAAATGGTCAAAACAT
TAACGTTATCGTTGATTCAGAAAGCAGCAGATTACAATTACTCCAACCATTTGCCCCATG
GGATAAGAAAGATTTAGTCGATATGCAAGTTCTCATCAAGGTTCAAGGTAAATGTACCAC
CGATCACATCTCTATGGCTGGTCCATGGTTGAAATACAGAGGTCATTTGGATAACATTTC
CAACAATATGTTAATTGGTGCTATCAACTCTGAAAACGGTAAAGCCAACGCTGTTCTCAA
TCAATTCACTGGTGAAATCGGTCCAGTCCCAACCGTCGCTCGTGACTACAAGAAACGTGG
TGTCAATTGGATCGTCGTTGGTGATGAAAACTATGGTGAAGGTTCATCTCGTGAACATGC
TGCCCTCGAACCACGTCATCTCGGTGGTAAAGCTATCCTCGTTAAATCATTTGCTCGTAT
CCACGAAACCAATCTCAAGAAACAAGGTATCTTACCATTAACCTTTGCTAACCCATCTGA
TTACGATAAAATCTCTGGTGACGATCGTACTTCAATCATTGGTCTCAAAGATCTTGCCCC
AGGTAAACAACTCACTCTCATTGTTAAATCTGCTAAACAAGGTTCTGAATTGAAATTAAA
GCTAATCACACCATGAATGCTGGTCAAATCGAAGGTTCAAAGCTGGTTCCGCTTTAAATT
ATATTAAATCAGAAAAAGCCAAAAAAAAT
Length of connected seq. 1339
Full length Seq ID -
Full length Seq. -
Length of full length seq. -