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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHJ861
Library VH
(Link to library)
Clone ID VHJ861
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHJ861P
(Link to Original site)
Representative DNA sequence 
>VHJ861 (VHJ861Q) /CSM/VH/VHJ8-C/VHJ861Q.Seq.d/
CAACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATXXXXXXXXXXCTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGC
TGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGG
TAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAA
AGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAA
CATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAG
AGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGC
CTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTAT
TGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCG
TATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTT
CACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTTGATCA
TTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCAC
CAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACT
CTAAACAATTTAAGTCGCACTTNAGTTTTCAAANATAGAATCAAATCTAAAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*NHLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---SSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSF
VGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPT
LLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti
*valxfsxiesnlkk
Translated Amino Acid sequence (All Frames) 
Frame A:
qlssakkvkykmvnftidqiraimdrrenirnmsviahvdhgkttyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvtlk---

---liqr*skssc*lsr**prmg*k*chehlvfrsrr*rcqsprqchqrcsife*n*rff
rwcfpmghqrrccl**kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpn
slrtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsirilrfhr*
ftfphcrssfptmcl*slgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnn
lsrtxvfkxrikskk

Frame B:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*h*r---

---SSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDSF
VGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPT
LLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTAD
LRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti
*valxfsxiesnlkk

Frame C:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*NHLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGNTED---

---hpkmiqklvltislittngikmmp*tfglsvqkvtvpisssmspkvfni*mklkils
lvlsngppkkvlfvmkt*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppql
s*nqst**kllpqktllvvftlysivvevlllvknvvsvphyslskliyqy*npsvspli
yvptlpvklshnvsliiglqlvlstkirkplksl*pperekv*pqksqiltnstknskqf
kshxsfqx*nqi*kk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHJ861 (VHJ861Q) /CSM/VH/VHJ8-C/VHJ861Q.Seq.d/                       2599   0.0
VHA509 (VHA509Q) /CSM/VH/VHA5-A/VHA509Q.Seq.d/                       2565   0.0
VHP730 (VHP730Q) /CSM/VH/VHP7-B/VHP730Q.Seq.d/                       1467   0.0
VHK162 (VHK162Q) /CSM/VH/VHK1-C/VHK162Q.Seq.d/                       1467   0.0
VHJ786 (VHJ786Q) /CSM/VH/VHJ7-D/VHJ786Q.Seq.d/                       1467   0.0
VHJ691 (VHJ691Q) /CSM/VH/VHJ6-D/VHJ691Q.Seq.d/                       1467   0.0
VHJ323 (VHJ323Q) /CSM/VH/VHJ3-A/VHJ323Q.Seq.d/                       1467   0.0
VHI690 (VHI690Q) /CSM/VH/VHI6-D/VHI690Q.Seq.d/                       1467   0.0
VHH822 (VHH822Q) /CSM/VH/VHH8-A/VHH822Q.Seq.d/                       1467   0.0
VHD569 (VHD569Q) /CSM/VH/VHD5-C/VHD569Q.Seq.d/                       1467   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442671) Dictyostelium discoideum cDNA clone:ddv50j16, 3' ...  1475   0.0    1
(BJ446334) Dictyostelium discoideum cDNA clone:ddv62l07, 3' ...  1467   0.0    1
(BJ443011) Dictyostelium discoideum cDNA clone:ddv51k15, 3' ...  1467   0.0    1
(BJ442764) Dictyostelium discoideum cDNA clone:ddv50l21, 3' ...  1467   0.0    1
(BJ442731) Dictyostelium discoideum cDNA clone:ddv50e24, 3' ...  1467   0.0    1
(BJ442218) Dictyostelium discoideum cDNA clone:ddv49n05, 3' ...  1467   0.0    1
(BJ442075) Dictyostelium discoideum cDNA clone:ddv48c24, 3' ...  1467   0.0    1
(BJ441254) Dictyostelium discoideum cDNA clone:ddv46l06, 3' ...  1467   0.0    1
(BJ438830) Dictyostelium discoideum cDNA clone:ddv38i17, 3' ...  1467   0.0    1
(BJ437359) Dictyostelium discoideum cDNA clone:ddv34c04, 3' ...  1467   0.0    1
dna update 2008.11.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   303   1e-80
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   301   3e-80
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   298   3e-79
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   297   7e-79
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   296   9e-79
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   296   9e-79
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   296   9e-79
(P05197) RecName: Full=Elongation factor 2;          Short=EF-2;...   296   9e-79
AJ278147_1(AJ278147|pid:none) Rattus norvegicus partial Ef-2 gen...   296   9e-79
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   296   9e-79
protein update 2009. 7.15
PSORT 

 psg: 0.70  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.28  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for VHJ861 is cyt
5' end seq. ID VHJ861F
5' end seq. 
>VHJ861F.Seq
CAACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGATNNNNNNNNNN
Length of 5' end seq. 618
3' end seq. ID VHJ861Z
3' end seq. 
>VHJ861Z.Seq
NNNNNNNNNNCTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGATAACC
ACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTG
CCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTCTT
TCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAG
GTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTG
GTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAA
CTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTA
TTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGGTT
CCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTG
ATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTTGATCATTGGGCTT
CAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGA
GAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAA
TTTAAGTCGCACTTNAGTTTTCAAANATAGAATCAAATCTAAAAAAAA
Length of 3' end seq. 768
Connected seq. ID VHJ861P
Connected seq. 
>VHJ861P.Seq
CAACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATT
CGTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGAT
CACGGTAAAACCACTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
CTGAAGAT----------CTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGC
TGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGG
TAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAA
AGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAA
CATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAG
AGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGC
CTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTAT
TGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCG
TATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTT
CACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTTGATCA
TTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCAC
CAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACT
CTAAACAATTTAAGTCGCACTTNAGTTTTCAAANATAGAATCAAATCTAAAAAAAA
Length of connected seq. 1366
Full length Seq ID -
Full length Seq. -
Length of full length seq. -