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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHJ842
Library VH
(Link to library)
Clone ID VHJ842
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13654-1|Contig-U16307-1
Original site URL
Representative seq. ID VHJ842P
(Link to Original site)
Representative DNA sequence 
>VHJ842 (VHJ842Q) /CSM/VH/VHJ8-B/VHJ842Q.Seq.d/
AAATTTTTTTTTCTAACACACAACAGACCATTTTTATTATCATATTATTATTAAAGGAAA
ATAATAGTAATAAAAGTAATAATAAAATGTCAGATTTTATTGGTTTATCAGATGCAGATA
ATTATGATAATATTGAAGAATCAAATGGAAATGAATACGTTATTGATGACAGTTTACCGT
TAATTGAAAAAATTAAAAAATATGTTAAAAGTGAATTAGTTCTTCATAGATTATATGTTA
TTAGAGAATTTTCTGATTTAATTAGAATTCAATTTGAACAAGCAAATGAAATATTAATAA
CATTTATAGAGGAAGCAGTAACAGATAATGAACCAGTAATTAGACAAGCATTAGTAGAAC
AAATTCCATCAATTAGTGAATGTTATATTCAATTTGGTAGAGAGGATGGTTATCAAAAAG
TATTGAAAAATCTATTACCAATTGTAGCTCAATTAACCACTGACAGAAATCCACAAGTTA
GAATGTCAGCCGTTGAATCATTACAAGATATGGCTAGAATCATTAGACATGAAGACATTG
AAGTTCATTTAATACCTTTTATTAAAAGTTTAGTTAATGATTCAACTGATGAAGAGCATA
GAGTTCAAGCTGCAAATCTTTGCXXXXXXXXXXTCANGATCATGATGATGNNNATTTCAG
NAGCTNAAATAATAATAATAATAATGATGATNATAATAGTAATGGTNTTNATAATAATAG
TNATNNTAATGATGATNATAATAATAATAATAATAATAATAATAATAATAATAATAATAA
TAATAATAATAATAATAATAATAATAATAATAATGAAAATAATATAGAAGATAATAATAA
TTTTAATAATAATAATAATAATACTGAAGTTTTAACGACAGAATAATAAAAATAATATTT
ATAATATATAAAATAAATAAATAATAATAAAAAATATT
sequence update 2002.10.25
Translated Amino Acid sequence 
IFFSNTQQTIFIIILLLKENNSNKSNNKMSDFIGLSDADNYDNIEESNGNEYVIDDSLPL
IEKIKKYVKSELVLHRLYVIREFSDLIRIQFEQANEILITFIEEAVTDNEPVIRQALVEQ
IPSISECYIQFGREDGYQKVLKNLLPIVAQLTTDRNPQVRMSAVESLQDMARIIRHEDIE
VHLIPFIKSLVNDSTDEEHRVQAANLC---

---XDHDDXXFXSXNNNNNNDDXNSNGXXNNSXXNDDXNNNNNNNNNNNNNNNNNNNNNN
NNNENNIEDNNNFNNNNNNTEVLTTE**k*yl*yik*innnkky
Translated Amino Acid sequence (All Frames) 
Frame A:
kffflthnrpfllsyyy*rkiivikviikcqillvyqmqiimiilknqmemntllmtvyr
*lkklknmlkvn*ffidymllenfli*lefnlnkqmky**hl*rkq*qimnq*ldkh**n
kfhqlvnvifnlvermvikky*kiyyql*ln*plteihklecqplnhykiwlesldmktl
kfi*ylllkv*lmiqlmksiefklqif---

---sxs**xxfqxlk*******x***wxx***xx***x**********************
****k*yrr***f******y*sfndriikiifiiykink***ki

Frame B:
nfff*httdhfyyhiiikgk****k***nvrfywfircr*l**y*rikwk*iry**qftv
n*kn*kic*k*isss*iicy*rif*fn*nsi*tsk*ninniyrgssnr**tsn*tsisrt
nsin**mlysiw*rgwlsksieksitncssinh*qksts*nvsr*iitryg*nh*t*rh*
ssfntfy*kfs**fn**ra*ssscksl---

---XDHDDXXFXSXNNNNNNDDXNSNGXXNNSXXNDDXNNNNNNNNNNNNNNNNNNNNNN
NNNENNIEDNNNFNNNNNNTEVLTTE**k*yl*yik*innnkky

Frame C:
IFFSNTQQTIFIIILLLKENNSNKSNNKMSDFIGLSDADNYDNIEESNGNEYVIDDSLPL
IEKIKKYVKSELVLHRLYVIREFSDLIRIQFEQANEILITFIEEAVTDNEPVIRQALVEQ
IPSISECYIQFGREDGYQKVLKNLLPIVAQLTTDRNPQVRMSAVESLQDMARIIRHEDIE
VHLIPFIKSLVNDSTDEEHRVQAANLC---

---ximmmxisxaxiiiiimmxiivmvxiiivxxmmxiiiiiiiiiiiiiiiiiiiiiii
iimkii*kiiiiliiiiiilkf*rqnnknniyni*nk*iiikni
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHJ842 (VHJ842Q) /CSM/VH/VHJ8-B/VHJ842Q.Seq.d/                        839   0.0
VHQ475 (VHQ475Q) /CSM/VH/VHQ4-D/VHQ475Q.Seq.d/                        831   0.0
VHG638 (VHG638Q) /CSM/VH/VHG6-B/VHG638Q.Seq.d/                        831   0.0
VHC277 (VHC277Q) /CSM/VH/VHC2-D/VHC277Q.Seq.d/                        763   0.0
VHH612 (VHH612Q) /CSM/VH/VHH6-A/VHH612Q.Seq.d/                        551   e-156
VHD303 (VHD303Q) /CSM/VH/VHD3-A/VHD303Q.Seq.d/                        129   8e-29
VSF252 (VSF252Q) /CSM/VS/VSF2-C/VSF252Q.Seq.d/                         46   0.001
VSD845 (VSD845Q) /CSM/VS/VSD8-B/VSD845Q.Seq.d/                         46   0.001
VSD104 (VSD104Q) /CSM/VS/VSD1-A/VSD104Q.Seq.d/                         46   0.001
SSM293 (SSM293Q) /CSM/SS/SSM2-D/SSM293Q.Seq.d/                         46   0.001
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ423751) Dictyostelium discoideum cDNA clone:ddv50d12, 5' ...   839   0.0    3
(BJ427847) Dictyostelium discoideum cDNA clone:ddv63f20, 5' ...   831   0.0    3
(BJ421960) Dictyostelium discoideum cDNA clone:ddv44k10, 5' ...   831   0.0    3
(BJ419356) Dictyostelium discoideum cDNA clone:ddv35i20, 5' ...   763   0.0    4
(BJ422490) Dictyostelium discoideum cDNA clone:ddv46g04, 5' ...   551   0.0    3
(AF482384) Dictyostelium discoideum ABC transporter AbcG5 (a...   103   2e-52  3
(BJ419743) Dictyostelium discoideum cDNA clone:ddv37f01, 5' ...   129   8e-50  3
(ED269978) AUAC-aaz70g11.b1 Ascaris suum whole genome shotgu...    54   0.010  1
(CP000825) Prochlorococcus marinus str. MIT 9215, complete g...    38   0.11   11
(CU693417) Pig DNA sequence *** SEQUENCING IN PROGRESS *** f...    48   0.60   1
dna update 2008.11.20
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM502236_67(AM502236|pid:none) Leishmania infantum chromosome 18.     114   5e-24
AJ851434_1(AJ851434|pid:none) Gallus gallus partial mRNA for hyp...    72   3e-11
CR857670_1(CR857670|pid:none) Pongo abelii mRNA; cDNA DKFZp469D1...    71   6e-11
BC168638_1(BC168638|pid:none) Xenopus tropicalis cDNA clone IMAG...    71   6e-11
(Q8K2V1) RecName: Full=Serine/threonine-protein phosphatase 4 re...    63   2e-08
BC029761_1(BC029761|pid:none) Mus musculus protein phosphatase 4...    63   2e-08
BC048878_1(BC048878|pid:none) Danio rerio protein phosphatase 4,...    62   4e-08
AB385198_1(AB385198|pid:none) Synthetic construct DNA, clone: pF...    60   8e-08
BC076964_1(BC076964|pid:none) Xenopus tropicalis protein phospha...    60   1e-07
(Q8VI02) RecName: Full=Serine/threonine-protein phosphatase 4 re...    60   1e-07
protein update 2009. 7.14
PSORT 

 psg: 1.01  gvh: 0.38  alm: 0.33  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 20.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHJ842 is cyt
5' end seq. ID VHJ842F
5' end seq. 
>VHJ842F.Seq
AAATTTTTTTTTCTAACACACAACAGACCATTTTTATTATCATATTATTATTAAAGGAAA
ATAATAGTAATAAAAGTAATAATAAAATGTCAGATTTTATTGGTTTATCAGATGCAGATA
ATTATGATAATATTGAAGAATCAAATGGAAATGAATACGTTATTGATGACAGTTTACCGT
TAATTGAAAAAATTAAAAAATATGTTAAAAGTGAATTAGTTCTTCATAGATTATATGTTA
TTAGAGAATTTTCTGATTTAATTAGAATTCAATTTGAACAAGCAAATGAAATATTAATAA
CATTTATAGAGGAAGCAGTAACAGATAATGAACCAGTAATTAGACAAGCATTAGTAGAAC
AAATTCCATCAATTAGTGAATGTTATATTCAATTTGGTAGAGAGGATGGTTATCAAAAAG
TATTGAAAAATCTATTACCAATTGTAGCTCAATTAACCACTGACAGAAATCCACAAGTTA
GAATGTCAGCCGTTGAATCATTACAAGATATGGCTAGAATCATTAGACATGAAGACATTG
AAGTTCATTTAATACCTTTTATTAAAAGTTTAGTTAATGATTCAACTGATGAAGAGCATA
GAGTTCAAGCTGCAAATCTTTGCNNNNNNNNNN
Length of 5' end seq. 633
3' end seq. ID VHJ842Z
3' end seq. 
>VHJ842Z.Seq
NNNNNNNNNNTCANGATCATGATGATGNNNATTTCAGNAGCTNAAATAATAATAATAATA
ATGATGATNATAATAGTAATGGTNTTNATAATAATAGTNATNNTAATGATGATNATAATA
ATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATAATA
ATAATAATAATGAAAATAATATAGAAGATAATAATAATTTTAATAATAATAATAATAATA
CTGAAGTTTTAACGACAGAATAATAAAAATAATATTTATAATATATAAAATAAATAAATA
ATAATAAAAAATATT
Length of 3' end seq. 315
Connected seq. ID VHJ842P
Connected seq. 
>VHJ842P.Seq
AAATTTTTTTTTCTAACACACAACAGACCATTTTTATTATCATATTATTATTAAAGGAAA
ATAATAGTAATAAAAGTAATAATAAAATGTCAGATTTTATTGGTTTATCAGATGCAGATA
ATTATGATAATATTGAAGAATCAAATGGAAATGAATACGTTATTGATGACAGTTTACCGT
TAATTGAAAAAATTAAAAAATATGTTAAAAGTGAATTAGTTCTTCATAGATTATATGTTA
TTAGAGAATTTTCTGATTTAATTAGAATTCAATTTGAACAAGCAAATGAAATATTAATAA
CATTTATAGAGGAAGCAGTAACAGATAATGAACCAGTAATTAGACAAGCATTAGTAGAAC
AAATTCCATCAATTAGTGAATGTTATATTCAATTTGGTAGAGAGGATGGTTATCAAAAAG
TATTGAAAAATCTATTACCAATTGTAGCTCAATTAACCACTGACAGAAATCCACAAGTTA
GAATGTCAGCCGTTGAATCATTACAAGATATGGCTAGAATCATTAGACATGAAGACATTG
AAGTTCATTTAATACCTTTTATTAAAAGTTTAGTTAATGATTCAACTGATGAAGAGCATA
GAGTTCAAGCTGCAAATCTTTGC----------TCANGATCATGATGATGNNNATTTCAG
NAGCTNAAATAATAATAATAATAATGATGATNATAATAGTAATGGTNTTNATAATAATAG
TNATNNTAATGATGATNATAATAATAATAATAATAATAATAATAATAATAATAATAATAA
TAATAATAATAATAATAATAATAATAATAATAATGAAAATAATATAGAAGATAATAATAA
TTTTAATAATAATAATAATAATACTGAAGTTTTAACGACAGAATAATAAAAATAATATTT
ATAATATATAAAATAAATAAATAATAATAAAAAATATT
Length of connected seq. 928
Full length Seq ID -
Full length Seq. -
Length of full length seq. -