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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO360
Library AF
(Link to library)
Clone ID AFO360
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12346-1
Original site URL
Representative seq. ID AFO360P
(Link to Original site)
Representative DNA sequence 
>AFO360 (AFO360Q) /CSM/AF/AFO3-C/AFO360Q.Seq.d/
CACTGTTGGCCTACTGGTATGAGTGTTTTTTACTCCAAATTAAAAATAAATACTACACAT
AACAAATTATTCAATAAATTATAATACAATCATATATTTATATATATATAATAAAATATG
AGTACAACCACAGCAACACCAACAACAACAACACCAACACCAACAACAACAACACCAACA
GATTCACTCTCAAATTATAAAGAGGATGAACTTCAAAATCTATCAGTTCAAAAATTAAAA
GAATTAATTACCAGTGGGGGTGGTAGTTATGCAAGTTGCTTTGAAAAAAGTGATTTTGTA
GAAAGAGCTAAAACTATACGTTCTTCAATGCAAGCATTAGCAAAACCATCCCATGAAACT
GTATATAAAGAAAGAATTGACTTTTATGAATTATTGGGTGTACCATCCACAGCAACAAAG
AATGAAATTACAAAAGCATATTATAAATTAGCAAAAGAATATCATCCAGATAANAACAAA
AATGATTTATATGCANGAGGAANTGTTTAAAAAAGTTAXXXXXXXXXXAANAANAGAGTA
TGTTTCNNCCNGGCTTGAAATTAATTTGGAAANTTGGTAGATTGGATATCGATAGTGTAG
TTAGAGAAGTATGTGAACGTGTTTTAGGTGCAAAAGGTGTAGAGAAAAGAATTTTAAAAC
AAAGAGTTGAAGCTGTTAAACTTTTAGGTAAAATCTTTGAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
tvgllv*vfftpn*k*ilhitnysinyntiiylyiynki*vqpqqhqqqqhqhqqqqhqq
ihsqiikrmnfkiyqfkn*kn*lpvgvvvmqvalkkvil*kelklyvlqckh*qnhpmkl
yikkeltfmnywvyhpqqqrmklqkhiin*QKNIIQIXTKMIYMXEEXFKKV---

---XXSMFXPGLKLIWKXGRLDIDSVVREVCERVLGAKGVEKRILKQRVEAVKLLGKIFE
Translated Amino Acid sequence (All Frames) 
Frame A:
hcwptgmsvfysklkintthnklfnkl*ynhifiyi**nmstttatpttttptpttttpt
dslsnykedelqnlsvqklkelitsgggsyascfeksdfveraktirssmqalakpshet
vykeridfyellgvpstatkneitkayyklakeyhpdxnkndlyaxgxv*ks---

---xxrvcfxxa*n*fgxlvdwisiv*lekyvnvf*vqkv*rkef*nkelkllnf*vksl

Frame B:
tvgllv*vfftpn*k*ilhitnysinyntiiylyiynki*vqpqqhqqqqhqhqqqqhqq
ihsqiikrmnfkiyqfkn*kn*lpvgvvvmqvalkkvil*kelklyvlqckh*qnhpmkl
yikkeltfmnywvyhpqqqrmklqkhiin*QKNIIQIXTKMIYMXEEXFKKV---

---xxeyvsxxleinlexw*igyr*cs*rsm*tcfrckrcreknfktks*sc*tfr*nl*

Frame C:
llaywyecfllqiknkyyt*qiiq*iiiqsyiyiyiikyeynhsntnnnntntnnnntnr
ftlkl*rg*tsksisskikrinyqwgw*lckll*kk*fcrks*nytffnasisktip*nc
i*rkn*ll*iigctihsnke*nyksil*iskrissr*xqk*ficxrxclkkl---

---XXSMFXPGLKLIWKXGRLDIDSVVREVCERVLGAKGVEKRILKQRVEAVKLLGKIFE
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO360 (AFO360Q) /CSM/AF/AFO3-C/AFO360Q.Seq.d/                        920   0.0
VFL394 (VFL394Q) /CSM/VF/VFL3-D/VFL394Q.Seq.d/                        642   0.0
VFL127 (VFL127Q) /CSM/VF/VFL1-B/VFL127Q.Seq.d/                        642   0.0
SFJ717 (SFJ717Q) /CSM/SF/SFJ7-A/SFJ717Q.Seq.d/                        642   0.0
SFJ452 (SFJ452Q) /CSM/SF/SFJ4-C/SFJ452Q.Seq.d/                        642   0.0
SFG682 (SFG682Q) /CSM/SF/SFG6-D/SFG682Q.Seq.d/                        642   0.0
SFE369 (SFE369Q) /CSM/SF/SFE3-C/SFE369Q.Seq.d/                        642   0.0
CHE470 (CHE470Q) /CSM/CH/CHE4-C/CHE470Q.Seq.d/                        642   0.0
CHC641 (CHC641Q) /CSM/CH/CHC6-B/CHC641Q.Seq.d/                        642   0.0
CHB272 (CHB272Q) /CSM/CH/CHB2-C/CHB272Q.Seq.d/                        642   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BH157484|BH157484.1 ENTRI87TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
AZ683723|AZ683723.1 ENTHN94TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
AZ545241|AZ545241.1 ENTEI38TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
AZ681035|AZ681035.1 ENTJN08TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
AA550161|AA550161.1 1275m3 gmbPfHB3.1, G. Roman Reddy Plasmodium falciparum genomic clone 1275m, DNA sequence.    58  2e-04  1
BH131742|BH131742.1 ENTNB40TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
BH154330|BH154330.1 ENTTQ61TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
AZ543842|AZ543842.1 ENTGD17TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
BH157531|BH157531.1 ENTSQ29TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
AZ527973|AZ527973.1 ENTBX19TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    58  2e-04  1
dna update 2003. 9.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BX072555_2(BX072555|pid:none) Zebrafish DNA sequence from clone ...    59   1e-07
BC055555_1(BC055555|pid:none) Danio rerio DnaJ (Hsp40) homolog, ...    59   1e-07
AL845363_2(AL845363|pid:none) Zebrafish DNA sequence from clone ...    59   1e-07
AK001333_1(AK001333|pid:none) Homo sapiens cDNA FLJ10471 fis, cl...    58   2e-07
BC161074_1(BC161074|pid:none) Xenopus tropicalis hypothetical pr...    58   2e-07
AY890460_1(AY890460|pid:none) Synthetic construct Homo sapiens c...    58   3e-07
AF411044_1(AF411044|pid:none) Homo sapiens DnaJ protein Tid-1 mR...    58   3e-07
AF061749_1(AF061749|pid:none) Homo sapiens tumorous imaginal dis...    58   3e-07
AF325535_1(AF325535|pid:none) Mus musculus tumorous imaginal dis...    57   5e-07
AK004575_1(AK004575|pid:none) Mus musculus adult male lung cDNA,...    57   5e-07
protein update 2009. 4.26
PSORT 

 psg: 0.68  gvh: 0.35  alm: 0.52  top: 0.53  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.12  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: nuclear
	 20.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  8.0 %: cytoskeletal

>> prediction for AFO360 is nuc
5' end seq. ID AFO360F
5' end seq. 
>AFO360F.Seq
CACTGTTGGCCTACTGGTATGAGTGTTTTTTACTCCAAATTAAAAATAAATACTACACAT
AACAAATTATTCAATAAATTATAATACAATCATATATTTATATATATATAATAAAATATG
AGTACAACCACAGCAACACCAACAACAACAACACCAACACCAACAACAACAACACCAACA
GATTCACTCTCAAATTATAAAGAGGATGAACTTCAAAATCTATCAGTTCAAAAATTAAAA
GAATTAATTACCAGTGGGGGTGGTAGTTATGCAAGTTGCTTTGAAAAAAGTGATTTTGTA
GAAAGAGCTAAAACTATACGTTCTTCAATGCAAGCATTAGCAAAACCATCCCATGAAACT
GTATATAAAGAAAGAATTGACTTTTATGAATTATTGGGTGTACCATCCACAGCAACAAAG
AATGAAATTACAAAAGCATATTATAAATTAGCAAAAGAATATCATCCAGATAANAACAAA
AATGATTTATATGCANGAGGAANTGTTTAAAAAAGTTA----------
Length of 5' end seq. 518
3' end seq. ID AFO360Z
3' end seq. 
>AFO360Z.Seq
----------AANAANAGAGTATGTTTCNNCCNGGCTTGAAATTAATTTGGAAANTTGGT
AGATTGGATATCGATAGTGTAGTTAGAGAAGTATGTGAACGTGTTTTAGGTGCAAAAGGT
GTAGAGAAAAGAATTTTAAAACAAAGAGTTGAAGCTGTTAAACTTTTAGGTAAAATCTTT
GAA
Length of 3' end seq. 173
Connected seq. ID AFO360P
Connected seq. 
>AFO360P.Seq
CACTGTTGGCCTACTGGTATGAGTGTTTTTTACTCCAAATTAAAAATAAATACTACACAT
AACAAATTATTCAATAAATTATAATACAATCATATATTTATATATATATAATAAAATATG
AGTACAACCACAGCAACACCAACAACAACAACACCAACACCAACAACAACAACACCAACA
GATTCACTCTCAAATTATAAAGAGGATGAACTTCAAAATCTATCAGTTCAAAAATTAAAA
GAATTAATTACCAGTGGGGGTGGTAGTTATGCAAGTTGCTTTGAAAAAAGTGATTTTGTA
GAAAGAGCTAAAACTATACGTTCTTCAATGCAAGCATTAGCAAAACCATCCCATGAAACT
GTATATAAAGAAAGAATTGACTTTTATGAATTATTGGGTGTACCATCCACAGCAACAAAG
AATGAAATTACAAAAGCATATTATAAATTAGCAAAAGAATATCATCCAGATAANAACAAA
AATGATTTATATGCANGAGGAANTGTTTAAAAAAGTTA----------AANAANAGAGTA
TGTTTCNNCCNGGCTTGAAATTAATTTGGAAANTTGGTAGATTGGATATCGATAGTGTAG
TTAGAGAAGTATGTGAACGTGTTTTAGGTGCAAAAGGTGTAGAGAAAAGAATTTTAAAAC
AAAGAGTTGAAGCTGTTAAACTTTTAGGTAAAATCTTTGAA
Length of connected seq. 691
Full length Seq ID -
Full length Seq. -
Length of full length seq. -