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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO359
Library AF
(Link to library)
Clone ID AFO359
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID AFO359Z
(Link to Original site)
Representative DNA sequence 
>AFO359 (AFO359Q) /CSM/AF/AFO3-C/AFO359Q.Seq.d/
XXXXXXXXXXCNCTCCNTTGAATTNTCAAACTAAANANATGGTAAACTNTGAATCCNTCG
AAAAAATNAAAAATGGTNTTATNATTATCAACGTTTCAAAAGGNGCTTTAGTTAANGCTT
TTNANGCAANTGTTGGTTTAAAATNTGGTAAAANCAGTTCCCTTGGNANGGATGTCTATG
AAAATGAAACCNATTACTTTTATCAANATCCCAATGGNTCNATCNTTAAANATGANAATC
NCNCTTTATTAATCTCTTAACCNAANGTTATGATCCCTTCCCNNCAAGCATGGNACNCTA
AAAAAGCCCTTTCTTGGANCTGNGGGACNTCCCCTCAAAANTTCGTTGATTTTAGATCNA
ATCNAATTAAAAAATCNAATTTAGTTAANAANCCNATTTCNTCCCCNCCNACCCCATTAA
TTNAAAAAAAAAAAAATAATTAAAAATAAAAAAAAATAAAAAAATTAAATCATTTT
sequence update 2001. 6. 1
Translated Amino Acid sequence 
---XPLNXQTKXMVNXESXEKXKNGXXIINVSKGALVXAFXAXVGLKXGKXSSLGXDVYE
NETXYFYQXPNGSIXKXXNXXLLIS*pxvmipsxqawxxkkalswxxgtspqxfvdfrsn
xikksnlvxxpisspptplixkkknn*k*kkikklnhf
Translated Amino Acid sequence (All Frames) 
Frame A:
---XPLNXQTKXMVNXESXEKXKNGXXIINVSKGALVXAFXAXVGLKXGKXSSLGXDVYE
NETXYFYQXPNGSIXKXXNXXLLIS*pxvmipsxqawxxkkalswxxgtspqxfvdfrsn
xikksnlvxxpisspptplixkkknn*k*kkikklnhf


Frame B:
---lx*ixklxxw*txnpskkxkmvlxlstfqkxl*lxlxxqxlv*nxvkxvplxxmsmk
mkpitfixipmxxslxmxixly*slnxxl*slpxkhgtlkkpflgxxgxplkxslildxi
xlknxi*lxxxfxpxxph*xkkkkiiknkkk*kn*ii


Frame C:
---sxexsn*xxgkl*ixrknxkwxyxyqrfkrxfs*xfxxxcwfkxw*xqfpwxgcl*k
*nxlllsxsqwxxx*x*xsxfinlltxxydpfpxsmxx*kspflxlxdxpskxr*f*ixs
n*kixfs*xxxfxpxxpinxkkkk*lkikknkkiksf
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO359 (AFO359Q) /CSM/AF/AFO3-C/AFO359Q.Seq.d/                        434   e-121
VFH856 (VFH856Q) /CSM/VF/VFH8-C/VFH856Q.Seq.d/                        392   e-108
SFE418 (SFE418Q) /CSM/SF/SFE4-A/SFE418Q.Seq.d/                        381   e-105
VSK319 (VSK319Q) /CSM/VS/VSK3-A/VSK319Q.Seq.d/                        377   e-103
VFG394 (VFG394Q) /CSM/VF/VFG3-D/VFG394Q.Seq.d/                        363   2e-99
VFF779 (VFF779Q) /CSM/VF/VFF7-D/VFF779Q.Seq.d/                        363   2e-99
VFC866 (VFC866Q) /CSM/VF/VFC8-C/VFC866Q.Seq.d/                        361   6e-99
VFG876 (VFG876Q) /CSM/VF/VFG8-D/VFG876Q.Seq.d/                        357   1e-97
VFE164 (VFE164Q) /CSM/VF/VFE1-C/VFE164Q.Seq.d/                        355   4e-97
VFB603 (VFB603Q) /CSM/VF/VFB6-A/VFB603Q.Seq.d/                        353   2e-96
own update 2004.12.25
Homology vs DNA 

 ***** No hits found ******

Lambda     K      H
    1.37    0.711     1.31

Matrix: blastn matrix:1 -3
Number of Hits to DB: 2402445
Number of Sequences: 24929324
Number of extensions: 2402445
Number of successful extensions: 541039
Number of sequences better than 10.0: 0
length of query: 476
length of database: P,631,589,963
effective HSP length: 22
effective length of query: 454
effective length of database: P,083,144,835
effective search space: 14565747755090
effective search space used: 14565747755090
T: 0
A: 0
X1: 6 (11.9 bits)
X2: 10 (19.8 bits)
S1: 12 (24.3 bits)
S2: 21 (42.1 bits)
[blastall] WARNING:  [000.000]  Reached max 200 HSPs in BlastSaveCurrentHsp, continuing with this limit

ANTI-DNA BLAST search was processed by blast@nig.ac.jp, National Institute of Genetics, Japan.







Dictyostelium discoideum cDNA Project Home
dna update 2003. 9.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54UF7) RecName: Full=Putative D-lactate dehydrogenase;        ...   154   7e-37
AP006841_1461(AP006841|pid:none) Bacteroides fragilis YCH46 DNA,...    74   2e-12
CP000117_2647(CP000117|pid:none) Anabaena variabilis ATCC 29413,...    71   1e-11
AB1814(AB1814) D-lactate dehydrogenase [imported] - Nostoc sp. (...    71   1e-11
CP000140_1028(CP000140|pid:none) Parabacteroides distasonis ATCC...    70   2e-11
CP000450_1261(CP000450|pid:none) Nitrosomonas eutropha C91, comp...    70   2e-11
CP000139_2426(CP000139|pid:none) Bacteroides vulgatus ATCC 8482,...    70   3e-11
CP000885_1901(CP000885|pid:none) Clostridium phytofermentans ISD...    69   7e-11
CP001037_2271(CP001037|pid:none) Nostoc punctiforme PCC 73102, c...    68   9e-11
AE015928_1575(AE015928|pid:none) Bacteroides thetaiotaomicron VP...    67   1e-10
protein update 2009. 4.26
PSORT 

 psg: 0.69  gvh: 0.48  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.20  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 32.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi

>> prediction for AFO359 is cyt
5' end seq. ID -
5' end seq. -
Length of 5' end seq. -
3' end seq. ID AFO359Z
3' end seq. 
>AFO359Z.Seq
----------CNCTCCNTTGAATTNTCAAACTAAANANATGGTAAACTNTGAATCCNTCG
AAAAAATNAAAAATGGTNTTATNATTATCAACGTTTCAAAAGGNGCTTTAGTTAANGCTT
TTNANGCAANTGTTGGTTTAAAATNTGGTAAAANCAGTTCCCTTGGNANGGATGTCTATG
AAAATGAAACCNATTACTTTTATCAANATCCCAATGGNTCNATCNTTAAANATGANAATC
NCNCTTTATTAATCTCTTAACCNAANGTTATGATCCCTTCCCNNCAAGCATGGNACNCTA
AAAAAGCCCTTTCTTGGANCTGNGGGACNTCCCCTCAAAANTTCGTTGATTTTAGATCNA
ATCNAATTAAAAAATCNAATTTAGTTAANAANCCNATTTCNTCCCCNCCNACCCCATTAA
TTNAAAAAAAAAAAAATAATTAAAAATAAAAAAAAATAAAAAAATTAAATCATTTT
Length of 3' end seq. 466
Connected seq. ID -
Connected seq. -
Length of connected seq. -
Full length Seq ID -
Full length Seq. -
Length of full length seq. -