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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHJ736
Library VH
(Link to library)
Clone ID VHJ736
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11490-1
Original site URL
Representative seq. ID VHJ736P
(Link to Original site)
Representative DNA sequence 
>VHJ736 (VHJ736Q) /CSM/VH/VHJ7-B/VHJ736Q.Seq.d/
CTCATTTTTTATAATTTGAACGTTCTCATTTTCATTTCCAAATTTTTTATATTTTTTTTT
TTAATTATTTTTTTTATTAATATCNCCTTAATAAANGGGGTAGGANATAAATAAATTTAA
AATATATATTTACATATNTAAAAAAAAAAATAAANGGTTTTTNTXXXXXXXXXXTTGGTA
AATCCAATACGTATCTATGATATGGGTAAAAAGAAATTACTTAGAAAATGTGAGACAAAG
AATCTACCAAATACAATCGTCAATATTCATAGTTTAGGCGACAGGTTGGTGGTTGGTGAC
ATCCAAGAGAGCATTCATTTCATAAAGTATAAACGATCAGAGAATATGTTATATGTGTTT
GCAGATGATTTAGCACCACGTTGGATGACTTCATCGGTTATGTTGGATTATGATACGGTT
GCGGGTGCCGATAAGTTTGGTAATATCTTTGTATTACGTTTACCATTACTAATCTCTGAT
GAGGTTGAAGAGGATCCAACTGGCACTAAATTGAAATTTGAAAGTGGCACTTTAAACGGC
GCCCCTCATAAATTGGACCATATAGCTAATTTCTTTGTTGGTGATACCGTTACAACTTTA
AATAAAACTTCATTGGTAGTCGGTGGACCAGAGGTTATACTATACACAACCATCAGTGGT
GCCATTGGTGCCTTAATCCCCTTCACATCAAGAGAGGACGTAGATTTCTTTTCAACATTA
GAAATGAATATGAGATCCGATTGTCTACCATTATGCGGTCGTGATCATCTTGCCTATCGT
TCCTATTATTTCCCAGTTAAAAATATTATCGATGGTGACCTTTGTGAACAATTCTCAACT
TTAAATTATCAAAAACAATTATCAATCTCTGAAGAATTATCAAGATCACCNTACGTAAGT
TATTAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
sffii*tfsfsfpnflyfff*lffllisp**xg*XINKFKIYIYIXKKKNKXFX---

---LVNPIRIYDMGKKKLLRKCETKNLPNTIVNIHSLGDRLVVGDIQESIHFIKYKRSEN
MLYVFADDLAPRWMTSSVMLDYDTVAGADKFGNIFVLRLPLLISDEVEEDPTGTKLKFES
GTLNGAPHKLDHIANFFVGDTVTTLNKTSLVVGGPEVILYTTISGAIGALIPFTSREDVD
FFSTLEMNMRSDCLPLCGRDHLAYRSYYFPVKNIIDGDLCEQFSTLNYQKQLSISEELSR
SPYVSY*
Translated Amino Acid sequence (All Frames) 
Frame A:
lifynlnvlifiskffifffliiffinixlixgvgxk*i*niylhx*kkk*xvf---

---LVNPIRIYDMGKKKLLRKCETKNLPNTIVNIHSLGDRLVVGDIQESIHFIKYKRSEN
MLYVFADDLAPRWMTSSVMLDYDTVAGADKFGNIFVLRLPLLISDEVEEDPTGTKLKFES
GTLNGAPHKLDHIANFFVGDTVTTLNKTSLVVGGPEVILYTTISGAIGALIPFTSREDVD
FFSTLEMNMRSDCLPLCGRDHLAYRSYYFPVKNIIDGDLCEQFSTLNYQKQLSISEELSR
SPYVSY*

Frame B:
sffii*tfsfsfpnflyfff*lffllisp**xg*XINKFKIYIYIXKKKNKXFX---

---w*iqyvsmiwvkrnylenvrqriyqiqssifiv*atgwwlvtskrafis*sindqri
cymclqmi*hhvg*lhrlcwimirlrvpislvislyyvyhy*slmrlkriqlaln*nlkv
al*taplinwti*lisllviplql*iklhw*svdqrlyytqpsvvplvp*spshqert*i
sfqh*k*i*dpivyhyavviilpivpiisqlkilsmvtfvnnsql*iiknnyqslknyqd
hxt*vik

Frame C:
hfl*fershfhfqifyifffnyffy*yxlnkxgrx*inlkyiftyxkkkixgfx---

---gksntyl*yg*keit*km*dkestkynrqys*frrqvggw*hprehsfhkv*tirey
vicvcr*fsttlddfigyvgl*ygcgcr*vw*ylcitftitnl**g*rgsnwh*iei*kw
hfkrrps*igpys*flcw*yrynfk*nfigsrwtrgytihnhqwchwclnplhikrgrrf
lfnirneyeirlstimrs*ssclsfllfps*kyyrw*pl*tilnfklsktiinl*riiki
txrkll
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHJ736 (VHJ736Q) /CSM/VH/VHJ7-B/VHJ736Q.Seq.d/                       1447   0.0
AHN409 (AHN409Q) /CSM/AH/AHN4-A/AHN409Q.Seq.d/                       1411   0.0
CHG222 (CHG222Q) /CSM/CH/CHG2-A/CHG222Q.Seq.d/                       1409   0.0
VFI115 (VFI115Q) /CSM/VF/VFI1-A/VFI115Q.Seq.d/                       1162   0.0
VHJ660 (VHJ660Q) /CSM/VH/VHJ6-C/VHJ660Q.Seq.d/                        105   1e-21
VFN374 (VFN374Q) /CSM/VF/VFN3-D/VFN374Q.Seq.d/                         42   0.015
VFI458 (VFI458Q) /CSM/VF/VFI4-C/VFI458Q.Seq.d/                         38   0.23
SHH117 (SHH117Q) /CSM/SH/SHH1-A/SHH117Q.Seq.d/                         38   0.23
CFB870 (CFB870Q) /CSM/CF/CFB8-C/CFB870Q.Seq.d/                         38   0.23
VSK207 (VSK207Q) /CSM/VS/VSK2-A/VSK207Q.Seq.d/                         36   0.90
own update 2003. 1. 1
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442585) Dictyostelium discoideum cDNA clone:ddv50h09, 3' ...  1447   0.0    1
(BJ355415) Dictyostelium discoideum cDNA clone:dda57b04, 3' ...  1403   0.0    1
(BJ379217) Dictyostelium discoideum cDNA clone:ddc34k06, 3' ...  1398   0.0    1
(BJ431959) Dictyostelium discoideum cDNA clone:ddv17m03, 3' ...  1162   0.0    1
(BJ423840) Dictyostelium discoideum cDNA clone:ddv50g16, 5' ...   105   3e-18  1
(BJ423768) Dictyostelium discoideum cDNA clone:ddv50h09, 5' ...   105   3e-18  1
(EC761723) PSE00000029 rw_mgpallid Polysphondylium pallidum ...    74   2e-10  2
(BJ337314) Dictyostelium discoideum cDNA clone:dda57b04, 5' ...    70   2e-07  1
(DV739558) ID0AFF13DE11CM1 ID0AFF Acyrthosiphon pisum cDNA c...    60   3e-06  2
(DV740356) ID0AFF1AG02CM1 ID0AFF Acyrthosiphon pisum cDNA cl...    60   3e-06  2
dna update 2008.11.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54SA7) RecName: Full=Probable splicing factor 3B subunit 3;         474   e-132
AL132954_10(AL132954|pid:none) Arabidopsis thaliana DNA chromoso...   315   1e-84
AP005474_22(AP005474|pid:none) Oryza sativa Japonica Group genom...   313   5e-84
AP008208_251(AP008208|pid:none) Oryza sativa (japonica cultivar-...   313   5e-84
(Q1LVE8) RecName: Full=Splicing factor 3B subunit 3;  &BX784024_...   312   1e-83
BC047171_1(BC047171|pid:none) Danio rerio splicing factor 3b, su...   312   1e-83
AC157349_12(AC157349|pid:none) Medicago truncatula clone mth2-13...   310   6e-83
AJ720930_1(AJ720930|pid:none) Gallus gallus mRNA for hypothetica...   303   4e-81
D87686_1(D87686|pid:none) Homo sapiens mRNA for KIAA0017 protein...   303   4e-81
BC000463_1(BC000463|pid:none) Homo sapiens splicing factor 3b, s...   303   4e-81
protein update 2009. 7.14
PSORT 

 psg: 0.65  gvh: 0.33  alm: 0.32  top: 0.53  tms: 0.00  mit: 0.29  mip: 0.05
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 20.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system

>> prediction for VHJ736 is cyt
5' end seq. ID VHJ736F
5' end seq. 
>VHJ736F.Seq
CTCATTTTTTATAATTTGAACGTTCTCATTTTCATTTCCAAATTTTTTATATTTTTTTTT
TTAATTATTTTTTTTATTAATATCNCCTTAATAAANGGGGTAGGANATAAATAAATTTAA
AATATATATTTACATATNTAAAAAAAAAAATAAANGGTTTTTNTNNNNNNNNNN
Length of 5' end seq. 174
3' end seq. ID VHJ736Z
3' end seq. 
>VHJ736Z.Seq
NNNNNNNNNNTTGGTAAATCCAATACGTATCTATGATATGGGTAAAAAGAAATTACTTAG
AAAATGTGAGACAAAGAATCTACCAAATACAATCGTCAATATTCATAGTTTAGGCGACAG
GTTGGTGGTTGGTGACATCCAAGAGAGCATTCATTTCATAAAGTATAAACGATCAGAGAA
TATGTTATATGTGTTTGCAGATGATTTAGCACCACGTTGGATGACTTCATCGGTTATGTT
GGATTATGATACGGTTGCGGGTGCCGATAAGTTTGGTAATATCTTTGTATTACGTTTACC
ATTACTAATCTCTGATGAGGTTGAAGAGGATCCAACTGGCACTAAATTGAAATTTGAAAG
TGGCACTTTAAACGGCGCCCCTCATAAATTGGACCATATAGCTAATTTCTTTGTTGGTGA
TACCGTTACAACTTTAAATAAAACTTCATTGGTAGTCGGTGGACCAGAGGTTATACTATA
CACAACCATCAGTGGTGCCATTGGTGCCTTAATCCCCTTCACATCAAGAGAGGACGTAGA
TTTCTTTTCAACATTAGAAATGAATATGAGATCCGATTGTCTACCATTATGCGGTCGTGA
TCATCTTGCCTATCGTTCCTATTATTTCCCAGTTAAAAATATTATCGATGGTGACCTTTG
TGAACAATTCTCAACTTTAAATTATCAAAAACAATTATCAATCTCTGAAGAATTATCAAG
ATCACCNTACGTAAGTTATTAAA
Length of 3' end seq. 743
Connected seq. ID VHJ736P
Connected seq. 
>VHJ736P.Seq
CTCATTTTTTATAATTTGAACGTTCTCATTTTCATTTCCAAATTTTTTATATTTTTTTTT
TTAATTATTTTTTTTATTAATATCNCCTTAATAAANGGGGTAGGANATAAATAAATTTAA
AATATATATTTACATATNTAAAAAAAAAAATAAANGGTTTTTNT----------TTGGTA
AATCCAATACGTATCTATGATATGGGTAAAAAGAAATTACTTAGAAAATGTGAGACAAAG
AATCTACCAAATACAATCGTCAATATTCATAGTTTAGGCGACAGGTTGGTGGTTGGTGAC
ATCCAAGAGAGCATTCATTTCATAAAGTATAAACGATCAGAGAATATGTTATATGTGTTT
GCAGATGATTTAGCACCACGTTGGATGACTTCATCGGTTATGTTGGATTATGATACGGTT
GCGGGTGCCGATAAGTTTGGTAATATCTTTGTATTACGTTTACCATTACTAATCTCTGAT
GAGGTTGAAGAGGATCCAACTGGCACTAAATTGAAATTTGAAAGTGGCACTTTAAACGGC
GCCCCTCATAAATTGGACCATATAGCTAATTTCTTTGTTGGTGATACCGTTACAACTTTA
AATAAAACTTCATTGGTAGTCGGTGGACCAGAGGTTATACTATACACAACCATCAGTGGT
GCCATTGGTGCCTTAATCCCCTTCACATCAAGAGAGGACGTAGATTTCTTTTCAACATTA
GAAATGAATATGAGATCCGATTGTCTACCATTATGCGGTCGTGATCATCTTGCCTATCGT
TCCTATTATTTCCCAGTTAAAAATATTATCGATGGTGACCTTTGTGAACAATTCTCAACT
TTAAATTATCAAAAACAATTATCAATCTCTGAAGAATTATCAAGATCACCNTACGTAAGT
TATTAAA
Length of connected seq. 897
Full length Seq ID -
Full length Seq. -
Length of full length seq. -