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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHJ654
Library VH
(Link to library)
Clone ID VHJ654
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHJ654P
(Link to Original site)
Representative DNA sequence 
>VHJ654 (VHJ654Q) /CSM/VH/VHJ6-C/VHJ654Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAXXXXXXXXXXATCTCAT
TGAAGCTGGTTCTGATATCTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGC
TGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGG
TAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAA
AGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAA
CATGAGAGGTATCCGTTTCAATCTTTATGGTGTCACCCTCCACACTGATGCCATCCACAG
AGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGC
CTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTAT
TGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCG
TATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTT
CACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCA
TTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCAC
CAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTGACAAATTCCACGAAAAACTC
AAACAATT
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLL---

---LIEAGSDISSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQY
LNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYGVTLHTDAIHRGGGQIIPTARRVLYA
AELTASPTLLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVL
ESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLTN
STKNSNN
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*---

---islklvlishpkmiqklvltislittngikmmp*tfglsvqkvtvpisssmspkvfn
i*mklkilslvlsngppkkvlfvmkt*evsvsifmvspstlmpstevvvkssqllvvysm
lln*lppqls*nqst**kllpqktllvvftlysivvevlllvknvvsvphyslskliyqy
*npsvspliyvptlpvklshnvssiiglqlvlstkirkplksl*pperekv*pqksqi*q
iprktqti

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflir---

---sh*swf*yliqr*skssc*lsr**prmg*k*chehlvfrsrr*rcqsprqchqrcsi
fe*n*rffrwcfpmghqrrccl**kherypfqslwchpph*chpqrwwsnhpncssctlc
c*inclpnslrtnllsrnycprkrywwyllctqss*rycyw*rtsyrfpiilcqssftsi
rilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*srfshqkekrfsprnprsdk
fheklkq

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLL---

---LIEAGSDISSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQY
LNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYGVTLHTDAIHRGGGQIIPTARRVLYA
AELTASPTLLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVL
ESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLTN
STKNSNN
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHJ654 (VHJ654Q) /CSM/VH/VHJ6-C/VHJ654Q.Seq.d/                       2415   0.0
VHK412 (VHK412Q) /CSM/VH/VHK4-A/VHK412Q.Seq.d/                       1435   0.0
VHC551 (VHC551Q) /CSM/VH/VHC5-C/VHC551Q.Seq.d/                       1421   0.0
VHI722 (VHI722Q) /CSM/VH/VHI7-A/VHI722Q.Seq.d/                       1413   0.0
VHB625 (VHB625Q) /CSM/VH/VHB6-B/VHB625Q.Seq.d/                       1413   0.0
VHA217 (VHA217Q) /CSM/VH/VHA2-A/VHA217Q.Seq.d/                       1413   0.0
CFC418 (CFC418Q) /CSM/CF/CFC4-A/CFC418Q.Seq.d/                       1407   0.0
VHN238 (VHN238Q) /CSM/VH/VHN2-B/VHN238Q.Seq.d/                       1398   0.0
VHJ678 (VHJ678Q) /CSM/VH/VHJ6-D/VHJ678Q.Seq.d/                       1394   0.0
VHN489 (VHN489Q) /CSM/VH/VHN4-D/VHN489Q.Seq.d/                       1392   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442674) Dictyostelium discoideum cDNA clone:ddv50k14, 3' ...  1457   0.0    1
(BJ442828) Dictyostelium discoideum cDNA clone:ddv51h04, 3' ...  1433   0.0    1
(BJ444764) Dictyostelium discoideum cDNA clone:ddv57k10, 3' ...  1398   0.0    1
(BJ442759) Dictyostelium discoideum cDNA clone:ddv50k20, 3' ...  1392   0.0    1
(BJ438155) Dictyostelium discoideum cDNA clone:ddv36e13, 3' ...  1392   0.0    2
(BJ444890) Dictyostelium discoideum cDNA clone:ddv57b24, 3' ...  1390   0.0    1
(BJ442507) Dictyostelium discoideum cDNA clone:ddv50h05, 3' ...  1390   0.0    1
(BJ445805) Dictyostelium discoideum cDNA clone:ddv60o15, 3' ...  1388   0.0    1
(BJ444935) Dictyostelium discoideum cDNA clone:ddv57m22, 3' ...  1388   0.0    1
(BJ444785) Dictyostelium discoideum cDNA clone:ddv57o10, 3' ...  1388   0.0    1
dna update 2008.11.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   298   3e-79
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   298   4e-79
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   294   5e-78
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   293   7e-78
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   293   7e-78
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   293   9e-78
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   293   9e-78
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   290   8e-77
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   290   8e-77
AK032997_1(AK032997|pid:none) Mus musculus 12 days embryo male w...   290   8e-77
protein update 2009. 7.14
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHJ654 is cyt
5' end seq. ID VHJ654F
5' end seq. 
>VHJ654F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGANNNNNNNNNN
Length of 5' end seq. 533
3' end seq. ID VHJ654Z
3' end seq. 
>VHJ654Z.Seq
NNNNNNNNNNATCTCATTGAAGCTGGTTCTGATATCTCATCCAAAGATGATCCAAAAGCT
CGTGCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGG
TCTTTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAA
TATTTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGT
GTTGTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGGTGTCACCCTCCAC
ACTGATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTAT
GCTGCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACT
GCCCCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTT
ATTGGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTA
TTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCA
CAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACT
GAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTGACA
AATTCCACGAAAAACTCAAACAATT
Length of 3' end seq. 745
Connected seq. ID VHJ654P
Connected seq. 
>VHJ654P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGA----------ATCTCAT
TGAAGCTGGTTCTGATATCTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGC
TGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGG
TAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAA
AGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAA
CATGAGAGGTATCCGTTTCAATCTTTATGGTGTCACCCTCCACACTGATGCCATCCACAG
AGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGC
CTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTAT
TGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCG
TATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTT
CACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCA
TTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCAC
CAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTGACAAATTCCACGAAAAACTC
AAACAATT
Length of connected seq. 1258
Full length Seq ID -
Full length Seq. -
Length of full length seq. -