>AFO339 (AFO339Q) /CSM/AF/AFO3-B/AFO339Q.Seq.d/
XXXXXXXXXXGACAAAATCATACGCACCTATTGGTGAAAATGGTATACCACGTACCGTAT
TAACTCATTATCCAATGGCTTACAATGTTGAAGATAGTGGTTTACCAGTGATTGATCAAA
CAGGTGTACCAAGATTATGTAAAGGTGAATTCAATTCTCGTGGTATCATCACCTTCAATT
CATTCATCCTAAAGGCAACAACTAAGCCTGCCGAATGTCCTTACATTGCCGCTGGTTTCT
TTTTCACCTCTGGTGAAGCAATTAAATTGGTACCATTTGATCCTCATCTTTCAAATCTAT
TCGAAGGTGAAGAAATTCTATACAGTGTTAGAATGTATTCAGCTGGATTTAGATTCTTTG
CTCCAACTCTCAACGTTTGTTTTCACTATTACTCTCGTCCAAAATCTCCAAAATTCTGGG
AAGATAATAAACAATACTATTTAGATATGCAAAAATCGGTTGAACGTTTAAAATACATCT
TACGTTGGCCTTTAGAGAATACTCCTGATCTCTCTGAACCACAATTTGCTGAAATCGATA
AATATACCATTGCTAATACATACCATTTTAACTGAATATTATCAACGTTGGAAAATTGAT
GTNAAAATAAAAAAGTACATTGGCATCAATTTTGGATCANCAACCTCCACAAGAATAAAT
TTTATAATATATAATAATAATAATAAAAAGA

---TKSYAPIGENGIPRTVLTHYPMAYNVEDSGLPVIDQTGVPRLCKGEFNSRGIITFNS
FILKATTKPAECPYIAAGFFFTSGEAIKLVPFDPHLSNLFEGEEILYSVRMYSAGFRFFA
PTLNVCFHYYSRPKSPKFWEDNKQYYLDMQKSVERLKYILRWPLENTPDLSEPQFAEIDK
YTIANTYHFN*ilstlen*cxnkkvhwhqfwixnlhknkfyni*****k

Frame A:
---TKSYAPIGENGIPRTVLTHYPMAYNVEDSGLPVIDQTGVPRLCKGEFNSRGIITFNS
FILKATTKPAECPYIAAGFFFTSGEAIKLVPFDPHLSNLFEGEEILYSVRMYSAGFRFFA
PTLNVCFHYYSRPKSPKFWEDNKQYYLDMQKSVERLKYILRWPLENTPDLSEPQFAEIDK
YTIANTYHFN*ilstlen*cxnkkvhwhqfwixnlhknkfyni*****k


Frame B:
---qnhthllvkmvyhvpy*liiqwltmlkivvyq*likqvyqdyvkvnsilvvsspsih
ss*rqqlslpnvltlplvsfsplvkqlnwyhlilifqiyskvkkfytvleciqldldsll
qlstfvftitlvqnlqnsgkiinnti*icknrlnv*ntsyvgl*rillislnhnllksin
ipllihtilteyyqrwkidvkikkyiginfgsxtstrinfiiynnnnkk


Frame C:
---kiirtyw*kwyttyrinslsnglqc*r*wftsd*snrctkim*r*iqfswyhhlqfi
hpkgnn*acrmslhcrwflfhlw*sn*igti*sssfksirr*rnsiqc*nvfswi*ilcs
nsqrlfslllsskiskilgr**tilfryakig*tfkihltlafreys*sl*ttic*nr*i
yhc*yipf*lniinvgklmxk*kstlasildxqppqe*il*yiiiiikr

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO339 (AFO339Q) /CSM/AF/AFO3-B/AFO339Q.Seq.d/                       1263   0.0
SFE430 (SFE430Q) /CSM/SF/SFE4-B/SFE430Q.Seq.d/                       1217   0.0
AFN357 (AFN357Q) /CSM/AF/AFN3-C/AFN357Q.Seq.d/                       1213   0.0
AFH874 (AFH874Q) /CSM/AF/AFH8-D/AFH874Q.Seq.d/                       1205   0.0
AFM626 (AFM626Q) /CSM/AF/AFM6-B/AFM626Q.Seq.d/                       1199   0.0
SLE662 (SLE662Q) /CSM/SL/SLE6-C/SLE662Q.Seq.d/                       1132   0.0
SSM226 (SSM226Q) /CSM/SS/SSM2-B/SSM226Q.Seq.d/                       1065   0.0
SLF365 (SLF365Q) /CSM/SL/SLF3-C/SLF365Q.Seq.d/                        797   0.0
SLF147 (SLF147Q) /CSM/SL/SLF1-B/SLF147Q.Seq.d/                        704   0.0
SLC474 (SLC474Q) /CSM/SL/SLC4-D/SLC474Q.Seq.d/                        670   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AF509501|AF509501.1 Dictyostelium discoideum putative polypeptide GlcNAc-transferase catalytic region (cis4c) gene, partial cds.  1088  0.0  4
AF233612|AF233612.1 Dictyostelium discoideum putative phosphatidylinositol 4-phosphate 5-kinase gene, partial sequence.  1041  0.0  1
AC006461|AC006461.2 Homo sapiens BAC clone RP11-343N14 from 2, complete sequence.    40  1.7  4
BQ024807|BQ024807.1 UI-1-BB1p-ats-f-06-0-UI.s1 NCI_CGAP_Pl6 Homo sapiens cDNA clone UI-1-BB1p-ats-f-06-0-UI 3', mRNA sequence.    34  2.1  2
AL929530|AL929530.9 Zebrafish DNA sequence from clone CH211-190H2.    44  2.3  1
AZ636334|AZ636334.1 1M0495D10F Mouse 10kb plasmid UUGC1M library Mus musculus genomic clone UUGC1M0495D10 F, DNA sequence.    44  2.3  1
AC026813|AC026813.3 Mus musculus chromosome 5 clone RP23-119M19 strain C57BL6/J, WORKING DRAFT SEQUENCE, 3 ordered pieces.    44  2.3  1
AK091619|AK091619.1 Homo sapiens cDNA FLJ34300 fis, clone FEBRA2006726.    34  6.6  2
AC142025|AC142025.1 Rattus norvegicus clone CH230-330B8, *** SEQUENCING IN PROGRESS ***, 43 unordered pieces.    42  8.9  1
BM146023|BM146023.1 TCAAP1D9588 Pediatric acute myelogenous leukemia cell (FAB M1) Baylor-HGSC project=TCAA Homo sapiens cDNA clone TCAAP9588, mRNA sequence.    42  8.9  1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF509501_1(AF509501|pid:none) Dictyostelium discoideum putative ...   397   e-109
FJ168556_15(FJ168556|pid:none) Bodo saltans clone fosmid 93E01, ...   121   2e-26
CP000078_25(CP000078|pid:none) Leishmania major strain Friedlin ...   101   2e-20
AM502220_71(AM502220|pid:none) Leishmania infantum chromosome 2.      101   2e-20
AC008031_11(AC008031|pid:none) Trypanosoma brucei chromosome 2 c...    94   4e-18
EU599318_1(EU599318|pid:none) Trypanosoma cruzi strain CL Brener...    92   2e-17
AM502235_115(AM502235|pid:none) Leishmania infantum chromosome 17.     84   5e-15
AC091655_22(AC091655|pid:none) Trypanosoma brucei chromosome 5 c...    82   1e-14
AM494954_113(AM494954|pid:none) Leishmania braziliensis chromoso...    82   2e-14
CP000720_1332(CP000720|pid:none) Yersinia pseudotuberculosis IP ...    74   3e-12

 psg: 0.69  gvh: 0.38  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  4.0 %: extracellular, including cell wall
	  4.0 %: endoplasmic reticulum

>> prediction for AFO339 is nuc

>AFO339Z.Seq
----------GACAAAATCATACGCACCTATTGGTGAAAATGGTATACCACGTACCGTAT
TAACTCATTATCCAATGGCTTACAATGTTGAAGATAGTGGTTTACCAGTGATTGATCAAA
CAGGTGTACCAAGATTATGTAAAGGTGAATTCAATTCTCGTGGTATCATCACCTTCAATT
CATTCATCCTAAAGGCAACAACTAAGCCTGCCGAATGTCCTTACATTGCCGCTGGTTTCT
TTTTCACCTCTGGTGAAGCAATTAAATTGGTACCATTTGATCCTCATCTTTCAAATCTAT
TCGAAGGTGAAGAAATTCTATACAGTGTTAGAATGTATTCAGCTGGATTTAGATTCTTTG
CTCCAACTCTCAACGTTTGTTTTCACTATTACTCTCGTCCAAAATCTCCAAAATTCTGGG
AAGATAATAAACAATACTATTTAGATATGCAAAAATCGGTTGAACGTTTAAAATACATCT
TACGTTGGCCTTTAGAGAATACTCCTGATCTCTCTGAACCACAATTTGCTGAAATCGATA
AATATACCATTGCTAATACATACCATTTTAACTGAATATTATCAACGTTGGAAAATTGAT
GTNAAAATAAAAAAGTACATTGGCATCAATTTTGGATCANCAACCTCCACAAGAATAAAT
TTTATAATATATAATAATAATAATAAAAAGA