>AFO333 (AFO333Q) /CSM/AF/AFO3-B/AFO333Q.Seq.d/
ATTGTCATAATAATATTATAAAATTCAAAGATGTCTAAAGTAGTCCGTAGTAGTAAATAT
CGTCATGTTTTTGCAGCACAACCAAAAAAAGAAGAGTGCTATCAAAACTTAAAAGTTACC
AAATCAGCATGGGATAGTAATTACGTTGCAGCCAACACTAGATACTTTGGTGTTATTTGG
GATGCTGCAGGTGGTGGTTCATTCGCTGTTATTCCACATGAGGCTTCAGGTAAAACCACA
TCAGTTCCATTATTCAATGGTCATAAGAGTGCAGTTTTAGATATTGCTTTCCACCCATTC
AATGAAAACCTCGTTGGTTCAGTTTCTGAAGATTGTAACATTTGTATTTGGGGTATCCCA
GAAGGTGGTTTAACCGACTCAATCTCAACCCCACTCCAAACTTTATCTGGTCACAAGAGA
AAGGTTGGTACCATCTCATTCAATCCAGTTGCTGATAATGTTGCCGTTACCTCATCTGGT
GATTTCTTAGTTAAAACTTGGGATGTTGAACAAGGTAAGAATTTAACCACTGTTGAAGGT
CACTCTGATATGATCACTTCATGCGAATGGAATCACAATGGTAGCCAAATCGTTACCACC
TGTAAAGATAAGAAAGCTCGTGTTTTCGATCCAXXXXXXXXXX

iviiil*NSKMSKVVRSSKYRHVFAAQPKKEECYQNLKVTKSAWDSNYVAANTRYFGVIW
DAAGGGSFAVIPHEASGKTTSVPLFNGHKSAVLDIAFHPFNENLVGSVSEDCNICIWGIP
EGGLTDSISTPLQTLSGHKRKVGTISFNPVADNVAVTSSGDFLVKTWDVEQGKNLTTVEG
HSDMITSCEWNHNGSQIVTTCKDKKARVFD---

Frame A:
iviiil*NSKMSKVVRSSKYRHVFAAQPKKEECYQNLKVTKSAWDSNYVAANTRYFGVIW
DAAGGGSFAVIPHEASGKTTSVPLFNGHKSAVLDIAFHPFNENLVGSVSEDCNICIWGIP
EGGLTDSISTPLQTLSGHKRKVGTISFNPVADNVAVTSSGDFLVKTWDVEQGKNLTTVEG
HSDMITSCEWNHNGSQIVTTCKDKKARVFD---


Frame B:
ls**yykiqrclk*svvvnivmflqhnqkkksaikt*klpnqhgivitlqptldtlvlfg
mlqvvvhsllfhmrlqvkphqfhysmvirvqf*illsthsmktslvqflkivtfvfgvsq
kvv*ptqsqphsklylvtrerlvpshsiqllimlplphlvis*lklgmlnkvri*pllkv
tli*slhangitmvakslppvkirklvfsi---


Frame C:
chnniikfkdv*ssp***isscfcsttkkrrvlsklksyqismg**lrcsqh*ilwcylg
ccrwwfircyst*gfr*nhissiiqws*ecsfrycfppiq*kprwfsf*rl*hlylgypr
rwfnrlnlnptpnfiwsqekgwyhliqssc**ccryliw*fls*nlgc*tr*efnhc*rs
l*ydhfmrmesqw*pnryhl*r*esscfrs---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VFL210 (VFL210Q) /CSM/VF/VFL2-A/VFL210Q.Seq.d/                       1213   0.0
VFH268 (VFH268Q) /CSM/VF/VFH2-C/VFH268Q.Seq.d/                       1213   0.0
VFC804 (VFC804Q) /CSM/VF/VFC8-A/VFC804Q.Seq.d/                       1213   0.0
SFE549 (SFE549Q) /CSM/SF/SFE5-C/SFE549Q.Seq.d/                       1213   0.0
SFC215 (SFC215Q) /CSM/SF/SFC2-A/SFC215Q.Seq.d/                       1213   0.0
AFO859 (AFO859Q) /CSM/AF/AFO8-C/AFO859Q.Seq.d/                       1213   0.0
AFO840 (AFO840Q) /CSM/AF/AFO8-B/AFO840Q.Seq.d/                       1213   0.0
AFO431 (AFO431Q) /CSM/AF/AFO4-B/AFO431Q.Seq.d/                       1213   0.0
AFO333 (AFO333Q) /CSM/AF/AFO3-B/AFO333Q.Seq.d/                       1213   0.0
AFK462 (AFK462Q) /CSM/AF/AFK4-C/AFK462Q.Seq.d/                       1213   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X61480|X61480.1 D.discoideum mRNA for coronin.                  1061  0.0  2
AZ542600|AZ542600.1 ENTET32TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36  0.043  2
BH132994|BH132994.1 ENTNE76TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    36  0.044  2
AC110563|AC110563.3 Mus musculus clone RP23-287O4, WORKING DRAFT SEQUENCE, 7 ordered pieces.    46  0.53  1
BZ396696|BZ396696.1 EINDS48TF EI_10_12_KB Entamoeba invadens genomic clone EINDS48, DNA sequence.    40  0.57  2
AC137559|AC137559.2 Homo sapiens clone RP11-344A9, *** SEQUENCING IN PROGRESS ***, 36 unordered pieces.    36  1.6  2
AZ675137|AZ675137.1 ENTJY92TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    44  2.1  1
AA750536|AA750536.1 ISGS0113 Rice Immature Seed Lambda ZAPII cDNA Library Oryza sativa cDNA clone ISGS0113, mRNA sequence.    36  2.4  2
AU030546|AU030546.1 Oryza sativa cDNA, partial sequence (E51225_1A).    36  3.5  2
AC022058|AC022058.3 Homo sapiens chromosome 8 clone RP11-482M5 map 8, WORKING DRAFT SEQUENCE, 8 unordered pieces.    36  5.3  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

S18663(S18663) coronin - slime mold (Dictyostelium discoideum)  ...   416   e-115
AY525781_1(AY525781|pid:none) Acanthamoeba healyi coronin mRNA, ...   229   6e-59
AP006852_172(AP006852|pid:none) Candida albicans genomic DNA, ch...   204   2e-51
FM992694_169(FM992694|pid:none) Candida dubliniensis CD36 chromo...   203   3e-51
BT042868_1(BT042868|pid:none) Zea mays full-length cDNA clone ZM...   200   3e-50
AE013599_228(AE013599|pid:none) Drosophila melanogaster chromoso...   197   2e-49
BC075371_1(BC075371|pid:none) Xenopus tropicalis coronin, actin ...   195   8e-49
AY330221_1(AY330221|pid:none) Danio rerio Coro1A mRNA, complete ...   195   1e-48
(Q06440) RecName: Full=Coronin-like protein;  &AY693023_1(AY6930...   189   4e-47
BT058865_1(BT058865|pid:none) Salmo salar clone ssal-rgf-508-379...   189   6e-47

 psg: 0.58  gvh: 0.32  alm: 0.42  top: 0.53  tms: 0.00  mit: 0.42  mip: 0.04
 nuc: 0.02  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: mitochondrial
	 36.0 %: nuclear
	 16.0 %: cytoplasmic
	  8.0 %: cytoskeletal

>> prediction for AFO333 is mit

>AFO333F.Seq
ATTGTCATAATAATATTATAAAATTCAAAGATGTCTAAAGTAGTCCGTAGTAGTAAATAT
CGTCATGTTTTTGCAGCACAACCAAAAAAAGAAGAGTGCTATCAAAACTTAAAAGTTACC
AAATCAGCATGGGATAGTAATTACGTTGCAGCCAACACTAGATACTTTGGTGTTATTTGG
GATGCTGCAGGTGGTGGTTCATTCGCTGTTATTCCACATGAGGCTTCAGGTAAAACCACA
TCAGTTCCATTATTCAATGGTCATAAGAGTGCAGTTTTAGATATTGCTTTCCACCCATTC
AATGAAAACCTCGTTGGTTCAGTTTCTGAAGATTGTAACATTTGTATTTGGGGTATCCCA
GAAGGTGGTTTAACCGACTCAATCTCAACCCCACTCCAAACTTTATCTGGTCACAAGAGA
AAGGTTGGTACCATCTCATTCAATCCAGTTGCTGATAATGTTGCCGTTACCTCATCTGGT
GATTTCTTAGTTAAAACTTGGGATGTTGAACAAGGTAAGAATTTAACCACTGTTGAAGGT
CACTCTGATATGATCACTTCATGCGAATGGAATCACAATGGTAGCCAAATCGTTACCACC
TGTAAAGATAAGAAAGCTCGTGTTTTCGATCCA----------