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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHJ558
Library VH
(Link to library)
Clone ID VHJ558
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15912-1
Original site URL
Representative seq. ID VHJ558P
(Link to Original site)
Representative DNA sequence 
>VHJ558 (VHJ558Q) /CSM/VH/VHJ5-C/VHJ558Q.Seq.d/
AAATTATTTTCTCCAAATTTATTTTTAATATACAAATAATATATATATAAAATGGCTACA
CCAGTCCAAGCTTCTACTGTAATTCGTGTTAAACCACATCATTATATTCATGTATTAGAT
AATAATACAAATGTTACAAGAGTTGAAGTTGGACCACAAACATTTACTAGACAAGATCAT
GAACGTGTATTAAATCCAACTCCATTACCAATGATTTTGATCCCACCAAGACAATATTGT
ATTATTGAAAACCCAATTATTAGAGATAAAGAAGGTAAATTAGTTGTTGATAAATTCAAT
CAACCAAAACTCCGTCATGGTGATCAAGAAATTCGTTTCTCTCAAGATCCATTCCCATTA
TATCCAGGTGAAGTTGTCATTGCTCAACCAGTTCCATTACAAGTCATCCAAACCAATAGT
GCACTTCGTTTGAAATGTCTTCGTGATTTCGTTGAAGTCAAAGCTGACAAAACCACAGTC
AGTCACATTGCTGGTGATGAATGGTTATTCGAAGGTCCAGGTACCTATTATCCACGTGTT
GAAGTTCAAGTTGTTGAACCAGTTCGTGCAGTTATCATCAAAGAAAATCAAGCACTCAAA
TTACGTGCTCGTCTCTCATTTGTTGACCGTCGTGATGTAGTTCGTCAAGTTGGXXXXXXX
XXXTGGTCATCNCAAACATTGATGTTCAATCTGTAGAACCAGTCGGATCAACGTACTCTT
GATTCTTTACAAAAATCTGTTCAATTAGCCATTGAAATTACCACCAAATCACAAGAAGCC
ACTGCTCGTCAAGAAGCTGAACGTTTAGAACAAATGGCTCGTGGTGAACTTGAACGTCAA
AAGATTATCGATGAAGCCAAGAACGAAGAATCTCGTTCCAAATTGGTCCAATTACAAGCT
CAATCAGCTGCTGTCGAATCAACTGGTCAAGCTGTTGCTGAAGCTCGTGCTCGTGCTGAA
GCCGCCATCATTGAAGCTGAAAGTGAAATCAAACAAGCTCGTCTCTCAACCAGAGCCATT
GAAATTGAAGCTCTCTCTGAAATTGAAAATCTTAAAGCCAAACATATTGTTGAAATCCAA
CACATCAAATCACTCAACAATCTCGAATTAATTAAAGCCAAAGAAAGTGCTACCATCGAA
ACTACCAAATTCGAAAACTATGTTGAAGCCCTCGGTTCCGATACTATCAAATCAATCGCC
CAAGCTCCAGAAGAAACCAAGGCTAAATTATTAGCTGGTTTGGGTCTCAAATCATTCATG
ATTACTGATGGTAAATCACCACTCAATCTTTTCGATACTGCCAATGGTTTAATTGCTGAT
sequence update 2002.10.25
Translated Amino Acid sequence 
klfspnlfliyk*YIYKMATPVQASTVIRVKPHHYIHVLDNNTNVTRVEVGPQTFTRQDH
ERVLNPTPLPMILIPPRQYCIIENPIIRDKEGKLVVDKFNQPKLRHGDQEIRFSQDPFPL
YPGEVVIAQPVPLQVIQTNSALRLKCLRDFVEVKADKTTVSHIAGDEWLFEGPGTYYPRV
EVQVVEPVRAVIIKENQALKLRARLSFVDRRDVVRQV---

---VIXNIDVQSVEPVGSTYS*fftkicsish*nyhqitrshcssrs*tfrtngsw*t*t
skdyr*sqerrisfqigpitssisccrinwsscc*sscsc*srhh*s*k*nqtssslnqs
h*n*ssl*n*ks*sqtyc*npthqitqqsrin*sqrkcyhrnyqirklc*sprfryyqin
rpssrrnqg*iiswfgsqiihdy*w*ittqsfrycqwfnc*
Translated Amino Acid sequence (All Frames) 
Frame A:
klfspnlfliyk*YIYKMATPVQASTVIRVKPHHYIHVLDNNTNVTRVEVGPQTFTRQDH
ERVLNPTPLPMILIPPRQYCIIENPIIRDKEGKLVVDKFNQPKLRHGDQEIRFSQDPFPL
YPGEVVIAQPVPLQVIQTNSALRLKCLRDFVEVKADKTTVSHIAGDEWLFEGPGTYYPRV
EVQVVEPVRAVIIKENQALKLRARLSFVDRRDVVRQV---

---wssqtlmfnl*nqsdqrtldslqksvqlaieittksqeatarqeaerleqmargele
rqkiideakneesrsklvqlqaqsaavestgqavaeararaeaaiieaeseikqarlstr
aieiealseienlkakhiveiqhikslnnlelikakesatiettkfenyvealgsdtiks
iaqapeetkakllaglglksfmitdgksplnlfdtangliad

Frame B:
nyflqiyf*ytnniyikwlhqsklll*fvlnhiiifmy*iiiqmlqelkldhkhlldkim
nvy*iqlhyq*f*shqdnivllktqlleikkvn*llinsinqnsvmvikkfvslkihshy
iqvklsllnqfhyksskpivhfv*nvfvislkskltkpqsvtllvmngyskvqvpiihvl
kfkllnqfvqlsskkikhsnyvlvshlltvvm*fvkl---

---ghxkh*csicrtsrinvllilyknlfn*plklppnhkkpllvkklnv*nkwlvvnln
vkrlsmkprtknlvpnwsnyklnqllsnqlvklllklvlvlkppslklkvksnklvsqpe
plklklslklkilkpnillksntsnhstisn*lkpkkvlpsklpnsktmlkpsvpilsnq
spklqkkprlny*lvwvsnhs*llmvnhhsifsilpmv*ll

Frame C:
iifskfifniqiiyi*ngytspsfycnsc*ttslyscir**ykcyks*swttniy*trs*
tciksnsitndfdptktilyy*kpny*r*rr*isc**iqstktpsw*srnsflsrsipii
sr*schcstssitshpnq*ctsfemss*fr*sqs*qnhsqshcw**mvirrsryllstc*
sssc*tsscsyhqrksstqitcsslic*ps*cssssw---

---VIXNIDVQSVEPVGSTYS*fftkicsish*nyhqitrshcssrs*tfrtngsw*t*t
skdyr*sqerrisfqigpitssisccrinwsscc*sscsc*srhh*s*k*nqtssslnqs
h*n*ssl*n*ks*sqtyc*npthqitqqsrin*sqrkcyhrnyqirklc*sprfryyqin
rpssrrnqg*iiswfgsqiihdy*w*ittqsfrycqwfnc*
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHJ558 (VHJ558Q) /CSM/VH/VHJ5-C/VHJ558Q.Seq.d/                       2551   0.0
AHL354 (AHL354Q) /CSM/AH/AHL3-C/AHL354Q.Seq.d/                       1294   0.0
VHJ686 (VHJ686Q) /CSM/VH/VHJ6-D/VHJ686Q.Seq.d/                       1281   0.0
VHI718 (VHI718Q) /CSM/VH/VHI7-A/VHI718Q.Seq.d/                       1281   0.0
VHI266 (VHI266Q) /CSM/VH/VHI2-C/VHI266Q.Seq.d/                       1281   0.0
AHL591 (AHL591Q) /CSM/AH/AHL5-D/AHL591Q.Seq.d/                       1273   0.0
VHG660 (VHG660Q) /CSM/VH/VHG6-C/VHG660Q.Seq.d/                       1271   0.0
SLB219 (SLB219Q) /CSM/SL/SLB2-A/SLB219Q.Seq.d/                       1265   0.0
SHL153 (SHL153Q) /CSM/SH/SHL1-C/SHL153Q.Seq.d/                       1265   0.0
AHC287 (AHC287Q) /CSM/AH/AHC2-D/AHC287Q.Seq.d/                       1265   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442636) Dictyostelium discoideum cDNA clone:ddv50c15, 3' ...  1296   0.0    1
(Z37109) D.discoideum mRNA for major vault protein B.            1219   0.0    2
(BJ442761) Dictyostelium discoideum cDNA clone:ddv50k22, 3' ...  1219   0.0    2
(BJ441840) Dictyostelium discoideum cDNA clone:ddv48d05, 3' ...  1219   0.0    2
(BJ441691) Dictyostelium discoideum cDNA clone:ddv47c18, 3' ...  1219   0.0    2
(BJ354446) Dictyostelium discoideum cDNA clone:dda53l13, 3' ...  1219   0.0    2
(AU033602) Dictyostelium discoideum slug cDNA, clone SLB219.     1211   0.0    2
(BJ354803) Dictyostelium discoideum cDNA clone:dda54e23, 3' ...  1211   0.0    2
(BJ423817) Dictyostelium discoideum cDNA clone:ddv50c15, 5' ...  1193   0.0    2
(BJ386616) Dictyostelium discoideum cDNA clone:ddc58h21, 3' ...  1193   0.0    1
dna update 2008.11.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P54659) RecName: Full=Major vault protein beta;          Short=...   358   2e-97
(Q62667) RecName: Full=Major vault protein;          Short=MVP; ...   197   7e-49
AK167899_1(AK167899|pid:none) Mus musculus TIB-55 BB88 cDNA, RIK...   197   9e-49
AK152534_1(AK152534|pid:none) Mus musculus bone marrow macrophag...   197   9e-49
BC049344_1(BC049344|pid:none) Danio rerio major vault protein, m...   197   9e-49
AK088046_1(AK088046|pid:none) Mus musculus 2 days neonate thymus...   196   1e-48
(Q9EQK5) RecName: Full=Major vault protein;          Short=MVP; ...   196   1e-48
(Q90405) RecName: Full=Major vault protein; AltName: Full=MVP100...   195   3e-48
S57723(S57723)lrp protein - human                                     194   5e-48
(Q14764) RecName: Full=Major vault protein;          Short=MVP; ...   194   5e-48
protein update 2009. 7.14
PSORT 

 psg: 0.83  gvh: 0.28  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.04
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: nuclear
	 24.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHJ558 is cyt
5' end seq. ID VHJ558F
5' end seq. 
>VHJ558F.Seq
AAATTATTTTCTCCAAATTTATTTTTAATATACAAATAATATATATATAAAATGGCTACA
CCAGTCCAAGCTTCTACTGTAATTCGTGTTAAACCACATCATTATATTCATGTATTAGAT
AATAATACAAATGTTACAAGAGTTGAAGTTGGACCACAAACATTTACTAGACAAGATCAT
GAACGTGTATTAAATCCAACTCCATTACCAATGATTTTGATCCCACCAAGACAATATTGT
ATTATTGAAAACCCAATTATTAGAGATAAAGAAGGTAAATTAGTTGTTGATAAATTCAAT
CAACCAAAACTCCGTCATGGTGATCAAGAAATTCGTTTCTCTCAAGATCCATTCCCATTA
TATCCAGGTGAAGTTGTCATTGCTCAACCAGTTCCATTACAAGTCATCCAAACCAATAGT
GCACTTCGTTTGAAATGTCTTCGTGATTTCGTTGAAGTCAAAGCTGACAAAACCACAGTC
AGTCACATTGCTGGTGATGAATGGTTATTCGAAGGTCCAGGTACCTATTATCCACGTGTT
GAAGTTCAAGTTGTTGAACCAGTTCGTGCAGTTATCATCAAAGAAAATCAAGCACTCAAA
TTACGTGCTCGTCTCTCATTTGTTGACCGTCGTGATGTAGTTCGTCAAGTTGGNNNNNNN
NNN
Length of 5' end seq. 663
3' end seq. ID VHJ558Z
3' end seq. 
>VHJ558Z.Seq
NNNNNNNNNNTGGTCATCNCAAACATTGATGTTCAATCTGTAGAACCAGTCGGATCAACG
TACTCTTGATTCTTTACAAAAATCTGTTCAATTAGCCATTGAAATTACCACCAAATCACA
AGAAGCCACTGCTCGTCAAGAAGCTGAACGTTTAGAACAAATGGCTCGTGGTGAACTTGA
ACGTCAAAAGATTATCGATGAAGCCAAGAACGAAGAATCTCGTTCCAAATTGGTCCAATT
ACAAGCTCAATCAGCTGCTGTCGAATCAACTGGTCAAGCTGTTGCTGAAGCTCGTGCTCG
TGCTGAAGCCGCCATCATTGAAGCTGAAAGTGAAATCAAACAAGCTCGTCTCTCAACCAG
AGCCATTGAAATTGAAGCTCTCTCTGAAATTGAAAATCTTAAAGCCAAACATATTGTTGA
AATCCAACACATCAAATCACTCAACAATCTCGAATTAATTAAAGCCAAAGAAAGTGCTAC
CATCGAAACTACCAAATTCGAAAACTATGTTGAAGCCCTCGGTTCCGATACTATCAAATC
AATCGCCCAAGCTCCAGAAGAAACCAAGGCTAAATTATTAGCTGGTTTGGGTCTCAAATC
ATTCATGATTACTGATGGTAAATCACCACTCAATCTTTTCGATACTGCCAATGGTTTAAT
TGCTGAT
Length of 3' end seq. 667
Connected seq. ID VHJ558P
Connected seq. 
>VHJ558P.Seq
AAATTATTTTCTCCAAATTTATTTTTAATATACAAATAATATATATATAAAATGGCTACA
CCAGTCCAAGCTTCTACTGTAATTCGTGTTAAACCACATCATTATATTCATGTATTAGAT
AATAATACAAATGTTACAAGAGTTGAAGTTGGACCACAAACATTTACTAGACAAGATCAT
GAACGTGTATTAAATCCAACTCCATTACCAATGATTTTGATCCCACCAAGACAATATTGT
ATTATTGAAAACCCAATTATTAGAGATAAAGAAGGTAAATTAGTTGTTGATAAATTCAAT
CAACCAAAACTCCGTCATGGTGATCAAGAAATTCGTTTCTCTCAAGATCCATTCCCATTA
TATCCAGGTGAAGTTGTCATTGCTCAACCAGTTCCATTACAAGTCATCCAAACCAATAGT
GCACTTCGTTTGAAATGTCTTCGTGATTTCGTTGAAGTCAAAGCTGACAAAACCACAGTC
AGTCACATTGCTGGTGATGAATGGTTATTCGAAGGTCCAGGTACCTATTATCCACGTGTT
GAAGTTCAAGTTGTTGAACCAGTTCGTGCAGTTATCATCAAAGAAAATCAAGCACTCAAA
TTACGTGCTCGTCTCTCATTTGTTGACCGTCGTGATGTAGTTCGTCAAGTTGG-------
---TGGTCATCNCAAACATTGATGTTCAATCTGTAGAACCAGTCGGATCAACGTACTCTT
GATTCTTTACAAAAATCTGTTCAATTAGCCATTGAAATTACCACCAAATCACAAGAAGCC
ACTGCTCGTCAAGAAGCTGAACGTTTAGAACAAATGGCTCGTGGTGAACTTGAACGTCAA
AAGATTATCGATGAAGCCAAGAACGAAGAATCTCGTTCCAAATTGGTCCAATTACAAGCT
CAATCAGCTGCTGTCGAATCAACTGGTCAAGCTGTTGCTGAAGCTCGTGCTCGTGCTGAA
GCCGCCATCATTGAAGCTGAAAGTGAAATCAAACAAGCTCGTCTCTCAACCAGAGCCATT
GAAATTGAAGCTCTCTCTGAAATTGAAAATCTTAAAGCCAAACATATTGTTGAAATCCAA
CACATCAAATCACTCAACAATCTCGAATTAATTAAAGCCAAAGAAAGTGCTACCATCGAA
ACTACCAAATTCGAAAACTATGTTGAAGCCCTCGGTTCCGATACTATCAAATCAATCGCC
CAAGCTCCAGAAGAAACCAAGGCTAAATTATTAGCTGGTTTGGGTCTCAAATCATTCATG
ATTACTGATGGTAAATCACCACTCAATCTTTTCGATACTGCCAATGGTTTAATTGCTGAT
Length of connected seq. 1310
Full length Seq ID -
Full length Seq. -
Length of full length seq. -