VHJ530 [Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview]

VHJ530
Library	VH (Link to library)
Clone ID	VHJ530 (Link to dictyBase)
Atlas ID	-
NBRP ID	-
dictyBase ID	-
Link to Contig	Contig-U16260-1
Original site URL	http://dictycdb.biol.tsukuba.ac.jp/CSM/VH/VHJ5-B/VHJ530Q.Seq.d/
Representative seq. ID	VHJ530P (Link to Original site)
Representative DNA sequence	>VHJ530 (VHJ530Q) /CSM/VH/VHJ5-B/VHJ530Q.Seq.d/ AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG TTTCTGGTGATATGAGATATATGTCACGTCGTGCTGATGAACAAGAGCGTGGTATCACCA TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG AAGAAGCTTATCTCTCTTXXXXXXXXXXATATCTCATCCAAAGATGATCCAAAAGCTCGT GCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCT TTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATAT TTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTT GTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACT GATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCT GCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCC CCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATT GGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTA GAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAA TGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAA GTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAA TTCCACGAAAAACTCTAAACAATTTAAGTCGCACTNAGTTTTC
sequence update	2002.10.25
Translated Amino Acid sequence	LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV SGDMRYMSRRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE EAYLS--- ---ISSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDS FVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASP TLLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTA DLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKLt ivalsf
Translated Amino Acid sequence (All Frames)	Frame A: nsrvqkkstkwlisqsikfvplwtvvkiseicllllmsitvkplyqilskelvllpik flvidichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnfsilsipqvtlis vqkllpsvllmvlslsiaskvfaykpklyyvkpslnvsnqsysstksivsynsntl kklisl--- ---ishpkmiqklvltislittngikmmptfglsvqkvtvpisssmspkvfnimklki lslvlsngppkkvlfvmktevsvsifmmspstlmpstevvvkssqllvvysmllnlpp qlsnqstkllpqktllvvftlysivvevlllvknvvsvphyslskliyqynpsvsp liyvptlpvklshnvssiiglqlvlstkirkplkslpperekvpqksqiltnstknsk qfkshxvf Frame B: tleckkskvqngfhnrsnschygpskyqkyvcycscrsrnhfirffnpkswyycrs fwyeiyvtsctrawyhhificitslrnakgrqitswlyftrifnqsyrfprsrfq frsnccppcywcfsryrlrrrclrtnrncitssrrtyqtslirqqsrsflirtpikh rslsl--- ---yliqrskssclsr*prmgkchehlvfrsrrrcqsprqchqrcsifenrf frwcfpmghqrrccl*kherypfqslchpphchpqrwwsnhpncssctlccinclp nslrtnllsrnycprkrywwyllctqssrycywrtsyrfpiilcqssftsirilrfhr ftfphcrssfptmclrslgfnwccqqreshsrfshqkekrfsprnprsqiprktln nlsrtxf Frame C: LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV SGDMRYMSRRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE EAYLS--- ---ISSKDDPKARANYLADNHEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQYLNEIKDS FVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASP TLLEPIYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIGSPLFSVKAHLPVLESFGFTA DLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKLt ivalsf
Homology vs CSM-cDNA	Score E Sequences producing significant alignments: (bits) Value VHJ530 (VHJ530Q) /CSM/VH/VHJ5-B/VHJ530Q.Seq.d/ 2478 0.0 VHK672 (VHK672Q) /CSM/VH/VHK6-C/VHK672Q.Seq.d/ 1451 0.0 VHJ671 (VHJ671Q) /CSM/VH/VHJ6-C/VHJ671Q.Seq.d/ 1451 0.0 VHI270 (VHI270Q) /CSM/VH/VHI2-C/VHI270Q.Seq.d/ 1451 0.0 VHJ541 (VHJ541Q) /CSM/VH/VHJ5-B/VHJ541Q.Seq.d/ 1445 0.0 VHD254 (VHD254Q) /CSM/VH/VHD2-C/VHD254Q.Seq.d/ 1445 0.0 VHE396 (VHE396Q) /CSM/VH/VHE3-D/VHE396Q.Seq.d/ 1443 0.0 VHD868 (VHD868Q) /CSM/VH/VHD8-C/VHD868Q.Seq.d/ 1443 0.0 VHC630 (VHC630Q) /CSM/VH/VHC6-B/VHC630Q.Seq.d/ 1443 0.0 VHA827 (VHA827Q) /CSM/VH/VHA8-B/VHA827Q.Seq.d/ 1443 0.0
own update	2004.12.24
Homology vs DNA	Score E Sequences producing significant alignments: (bits) Value N (BJ443368) Dictyostelium discoideum cDNA clone:ddv52o18, 3' ... 1451 0.0 1 (BJ442687) Dictyostelium discoideum cDNA clone:ddv50m18, 3' ... 1451 0.0 1 (BJ442596) Dictyostelium discoideum cDNA clone:ddv50k07, 3' ... 1451 0.0 1 (BJ441725) Dictyostelium discoideum cDNA clone:ddv47k18, 3' ... 1451 0.0 1 (BJ442548) Dictyostelium discoideum cDNA clone:ddv50a11, 3' ... 1445 0.0 1 (BJ438342) Dictyostelium discoideum cDNA clone:ddv37k14, 3' ... 1445 0.0 1 (BJ439284) Dictyostelium discoideum cDNA clone:ddv39p23, 3' ... 1443 0.0 1 (BJ438827) Dictyostelium discoideum cDNA clone:ddv38h18, 3' ... 1443 0.0 1 (BJ438107) Dictyostelium discoideum cDNA clone:ddv36k08, 3' ... 1443 0.0 1 (BJ436812) Dictyostelium discoideum cDNA clone:ddv32f08, 3' ... 1443 0.0 1
dna update	2008.11.18
Homology vs Protein	Score E Sequences producing significant alignments: (bits) Value AY391422_1(AY391422\|pid:none) Danio rerio eukaryotic translation... 304 4e-81 AY069933_1(AY069933\|pid:none) Rana sylvatica eukaryotic translat... 303 1e-80 BC089730_1(BC089730\|pid:none) Xenopus tropicalis MGC108369 prote... 299 1e-79 BT045031_1(BT045031\|pid:none) Salmo salar clone ssal-rgf-510-120... 298 3e-79 BC060707_1(BC060707\|pid:none) Mus musculus eukaryotic translatio... 298 4e-79 AK161993_1(AK161993\|pid:none) Mus musculus 12 days embryo male w... 298 4e-79 AK032997_1(AK032997\|pid:none) Mus musculus 12 days embryo male w... 298 4e-79 (P05197) RecName: Full=Elongation factor 2; Short=EF-2;... 298 4e-79 AJ278147_1(AJ278147\|pid:none) Rattus norvegicus partial Ef-2 gen... 298 4e-79 BT071866_1(BT071866\|pid:none) Salmo salar clone ssal-rgf-501-088... 298 4e-79
protein update	2009. 7.14
PSORT	psg: 0.71 gvh: 0.38 alm: 0.34 top: 0.53 tms: 0.00 mit: 0.30 mip: 0.00 nuc: 0.04 erl: 0.00 erm: 0.20 pox: 0.00 px2: 0.00 vac: 0.00 rnp: 0.00 act: 0.00 caa: 0.00 yqr: 0.00 tyr: 0.00 leu: 0.00 gpi: 0.00 myr: 0.00 dna: 0.00 rib: 0.00 bac: 0.00 m1a: 0.00 m1b: 0.00 m2 : 0.00 mNt: 0.00 m3a: 0.00 m3b: 0.00 m_ : 1.00 52.0 %: cytoplasmic 36.0 %: nuclear 4.0 %: extracellular, including cell wall 4.0 %: cytoskeletal 4.0 %: vacuolar >> prediction for VHJ530 is cyt
5' end seq. ID	VHJ530F
5' end seq.	>VHJ530F.Seq AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG TTTCTGGTGATATGAGATATATGTCACGTCGTGCTGATGAACAAGAGCGTGGTATCACCA TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG AAGAAGCTTATCTCTCTTNNNNNNNNNN
Length of 5' end seq.	568
3' end seq. ID	VHJ530Z
3' end seq.	>VHJ530Z.Seq NNNNNNNNNNATATCTCATCCAAAGATGATCCAAAAGCTCGTGCTAACTATCTCGCTGAT AACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAAC GGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGAT TCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATG AGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGT GGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCC CCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGT GGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATC GGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACC GCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGG GCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGA AAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAA ACAATTTAAGTCGCACTNAGTTTTC
Length of 3' end seq.	745
Connected seq. ID	VHJ530P
Connected seq.	>VHJ530P.Seq AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG TTTCTGGTGATATGAGATATATGTCACGTCGTGCTGATGAACAAGAGCGTGGTATCACCA TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG AAGAAGCTTATCTCTCTT----------ATATCTCATCCAAAGATGATCCAAAAGCTCGT GCTAACTATCTCGCTGATAACCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCT TTCGGTCCAGAAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAATAT TTGAATGAAATTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTT GTTTGTGATGAAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACT GATGCCATCCACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCT GCTGAATTAACTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCC CCAGAAAACGCTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATT GGTGAAGAACGTCGTATCGGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTA GAATCCTTCGGTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAA TGTGTCTTCGATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAA GTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAA TTCCACGAAAAACTCTAAACAATTTAAGTCGCACTNAGTTTTC
Length of connected seq.	1293
Full length Seq ID	-
Full length Seq.	-
Length of full length seq.	-

Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview

Dicty_cDB

Atlas

Dicty_cDB - Dictyostelium discoideum cDNA Clone Overview

Dicty_cDB

Atlas