NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHJ514
Library VH
(Link to library)
Clone ID VHJ514
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHJ514P
(Link to Original site)
Representative DNA sequence 
>VHJ514 (VHJ514Q) /CSM/VH/VHJ5-A/VHJ514Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGXXXXXXXXXXCCACGAAT
GGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATC
TCCTCGTCAATGTCACCAAAGGTGTTCAACATTTGAATGAAATTAAAGATTCTTTCGTTG
GTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCC
GTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAA
TCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCT
TAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACT
CTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCAGTTCCCCAT
TATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTAC
GTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTG
GTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAG
GTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAG
TCGCACTNAGTTTTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLL---

---HEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQHLNEIKDSFVGAFQWATKEGVVCDE
NMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPENA
IGGIYSVLNRRRGIVIGEERRISSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFD
HWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*valsfq
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*---

---prmg*k*chehlvfrsrr*rcqsprqchqrcstfe*n*rffrwcfpmghqrrccl**
kherypfqsl*chpph*chpqrwwsnhpncssctlcc*inclpnslrtnllsrnycprkr
ywwyllctqss*rycyw*rtsyqfpiilcqssftsirilrfhr*ftfphcrssfptmclr
slgfnwccqqr*esh*srfshqkekrfsprnprs*qiprktlnnlsrtxfs

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsfli---

---HEWDKNDAMNIWSFGPEGNGANLLVNVTKGVQHLNEIKDSFVGAFQWATKEGVVCDE
NMRGIRFNLYDVTLHTDAIHRGGGQIIPTARRVLYAAELTASPTLLEPIYLVEITAPENA
IGGIYSVLNRRRGIVIGEERRISSPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFD
HWASIGVVNKDKKATEVALATRKRKGLAPEIPDLDKFHEKL*ti*valsfq

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLL---

---tngikmmp*tfglsvqkvtvpisssmspkvfni*mklkilslvlsngppkkvlfvmk
t*evsvsifmmspstlmpstevvvkssqllvvysmlln*lppqls*nqst**kllpqktl
lvvftlysivvevlllvknvvsvphyslskliyqy*npsvspliyvptlpvklshnvssi
iglqlvlstkirkplksl*pperekv*pqksqiltnstknskqfkshxvfk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHJ514 (VHJ514Q) /CSM/VH/VHJ5-A/VHJ514Q.Seq.d/                       2309   0.0
VHO711 (VHO711Q) /CSM/VH/VHO7-A/VHO711Q.Seq.d/                       1338   0.0
VHK740 (VHK740Q) /CSM/VH/VHK7-B/VHK740Q.Seq.d/                       1338   0.0
VHK672 (VHK672Q) /CSM/VH/VHK6-C/VHK672Q.Seq.d/                       1338   0.0
VHK338 (VHK338Q) /CSM/VH/VHK3-B/VHK338Q.Seq.d/                       1338   0.0
VHK322 (VHK322Q) /CSM/VH/VHK3-A/VHK322Q.Seq.d/                       1338   0.0
VHJ680 (VHJ680Q) /CSM/VH/VHJ6-D/VHJ680Q.Seq.d/                       1338   0.0
VHJ671 (VHJ671Q) /CSM/VH/VHJ6-C/VHJ671Q.Seq.d/                       1338   0.0
VHJ541 (VHJ541Q) /CSM/VH/VHJ5-B/VHJ541Q.Seq.d/                       1338   0.0
VHI270 (VHI270Q) /CSM/VH/VHI2-C/VHI270Q.Seq.d/                       1338   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442519) Dictyostelium discoideum cDNA clone:ddv50k03, 3' ...  1354   0.0    1
(BJ445614) Dictyostelium discoideum cDNA clone:ddv60f03, 3' ...  1338   0.0    1
(BJ443368) Dictyostelium discoideum cDNA clone:ddv52o18, 3' ...  1338   0.0    1
(BJ443281) Dictyostelium discoideum cDNA clone:ddv52p09, 3' ...  1338   0.0    1
(BJ442939) Dictyostelium discoideum cDNA clone:ddv51l09, 3' ...  1338   0.0    1
(BJ442852) Dictyostelium discoideum cDNA clone:ddv51l05, 3' ...  1338   0.0    1
(BJ442780) Dictyostelium discoideum cDNA clone:ddv50o20, 3' ...  1338   0.0    1
(BJ442687) Dictyostelium discoideum cDNA clone:ddv50m18, 3' ...  1338   0.0    1
(BJ442548) Dictyostelium discoideum cDNA clone:ddv50a11, 3' ...  1338   0.0    1
(BJ441725) Dictyostelium discoideum cDNA clone:ddv47k18, 3' ...  1338   0.0    1
dna update 2008.11.18
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   285   3e-75
AY069933_1(AY069933|pid:none) Rana sylvatica eukaryotic translat...   283   7e-75
BT071866_1(BT071866|pid:none) Salmo salar clone ssal-rgf-501-088...   282   2e-74
BT059038_1(BT059038|pid:none) Salmo salar clone ssal-rgf-521-328...   282   2e-74
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   281   3e-74
BT045031_1(BT045031|pid:none) Salmo salar clone ssal-rgf-510-120...   281   3e-74
BT072304_1(BT072304|pid:none) Salmo salar clone ssal-rgf-520-280...   281   4e-74
BC060707_1(BC060707|pid:none) Mus musculus eukaryotic translatio...   279   1e-73
AK161993_1(AK161993|pid:none) Mus musculus 12 days embryo male w...   279   1e-73
AK298498_1(AK298498|pid:none) Homo sapiens cDNA FLJ56548 complet...   279   1e-73
protein update 2009. 7.14
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHJ514 is cyt
5' end seq. ID VHJ514F
5' end seq. 
>VHJ514F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGNNNNNNNNNN
Length of 5' end seq. 532
3' end seq. ID VHJ514Z
3' end seq. 
>VHJ514Z.Seq
NNNNNNNNNNCCACGAATGGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAG
AAGGTAACGGTGCCAATCTCCTCGTCAATGTCACCAAAGGTGTTCAACATTTGAATGAAA
TTAAAGATTCTTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATG
AAAACATGAGAGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCC
ACAGAGGTGGTGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAA
CTGCCTCCCCAACTCTCTTAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACG
CTATTGGTGGTATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAAC
GTCGTATCAGTTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCG
GTTTCACCGCTGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCG
ATCATTGGGCTTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAG
CCACCAGAAAGAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAA
AACTCTAAACAATTTAAGTCGCACTNAGTTTTCAAA
Length of 3' end seq. 696
Connected seq. ID VHJ514P
Connected seq. 
>VHJ514P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAG----------CCACGAAT
GGGATAAAAATGATGCCATGAACATTTGGTCTTTCGGTCCAGAAGGTAACGGTGCCAATC
TCCTCGTCAATGTCACCAAAGGTGTTCAACATTTGAATGAAATTAAAGATTCTTTCGTTG
GTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAGAGGTATCC
GTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGGTGGTCAAA
TCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCCAACTCTCT
TAGAACCAATCTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGGTATTTACT
CTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCAGTTCCCCAT
TATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTAC
GTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTG
GTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAG
GTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAACAATTTAAG
TCGCACTNAGTTTTCAAA
Length of connected seq. 1208
Full length Seq ID -
Full length Seq. -
Length of full length seq. -