>AFO328 (AFO328Q) /CSM/AF/AFO3-B/AFO328Q.Seq.d/
CACTGTTGGCCTACTGGAATTATAACCTTTCACATGATATAGTCGACAATATTTGAAGTC
ATTCAAATAATTTTTAATAAACAAAAATAAAATTTAATAAAATAAAATTTATAGAAAACA
ATATAAAATAAAAACAAAAAACAAAGTAATAAAAATATTAAAAAAAAAAXXXXXXXXXXG
NNNNGNNNNNNNTNNNNNTTNNTNNNTTNNTNNNNATAGTNNTNATNNAGTTNTTNNNNN
NNNTATTNNNNTGGTTATTANNNATNNNNCNNTTNANAGTNNAAANAGTNANNATTCATT
ATTTAATATTGANCNTTCNNTTGGTATTGTTNNNNCAATGNTTAGTANANNTGNTNCNTT
AAGANCTANAGNCAGTAGATCNAATAGAAATNCAATGGATCNTGAATTATTCTCTTCACT
TCAACCATTAACTCCAACTTCAAAACATACTACTACCNCTGCAACTAATACAAATACAAA
TACAAACACATGAATTAGATTCATTATTGAAACCAATAGCTGCATTATATGATAGAGCAA
TTAAATGGAAATCTGAATCAAATTCATCCTCATCTTCATCAGCATTAAATCAATCAAATG
GTAGTGGTAATGAACAAGTTGTTGCTGCACCTTCTGTT

tvgllel*pft*ysrqylksfk*flinknki**NKIYRKQYKIKTKNKVIKILKKK---

---XXXXXXXXXXXXSXXXVXXXXIXXVIXXXXXXSXXSXXSLFNIXXSXGIVXXMXSXX
XXLRXXXSRSNRNXMDXELFSSLQPLTPTSKHTTTXATNTNTNTNT*irfiietnscii*
*sn*mei*ikfilifisiksikw*w**tsccctfc

Frame A:
hcwptgiitfhmi*stifeviqiifnkqk*nlik*nl*kti*nknkkqsnknikkk---

---xxxxxxxxxxxxivxxxxxxxxxxwllxxxxxxvxxvxihylilxxxlvlxxqxlvx
xxx*xlxxvdxiexqwixnyslhfnh*lqlqnillplqliqiqiqtheldsllkpiaaly
draikwksesnssssssalnqsngsgneqvvaapsv

Frame B:
tvgllel*pft*ysrqylksfk*flinknki**NKIYRKQYKIKTKNKVIKILKKK---

---xxxxxxxxxxxx*xxxsxxxxyxxgyxxxxxxxxkxxxfii*y*xfxwycxxnx*xx
xxxkxxxq*ix*kxngs*iilftstinsnfktyyyxcn*ykykykhmn*ihy*nq*lhym
ieqlngnlnqihphlhqh*inqmvvvmnklllhll

Frame C:
llaywnynlshdivdni*shsnnf**tkikfnkikfiennik*kqktk**ky*kk---

---XXXXXXXXXXXXSXXXVXXXXIXXVIXXXXXXSXXSXXSLFNIXXSXGIVXXMXSXX
XXLRXXXSRSNRNXMDXELFSSLQPLTPTSKHTTTXATNTNTNTNT*irfiietnscii*
*sn*mei*ikfilifisiksikw*w**tsccctfc

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO328 (AFO328Q) /CSM/AF/AFO3-B/AFO328Q.Seq.d/                        553   e-156
AFO344 (AFO344Q) /CSM/AF/AFO3-B/AFO344Q.Seq.d/                        496   e-139
VFH665 (VFH665Q) /CSM/VF/VFH6-C/VFH665Q.Seq.d/                        301   7e-81
AFA337 (AFA337Q) /CSM/AF/AFA3-B/AFA337Q.Seq.d/                        230   2e-59
CFB411 (CFB411Q) /CSM/CF/CFB4-A/CFB411Q.Seq.d/                        129   6e-29
VSJ310 (VSJ310Q) /CSM/VS/VSJ3-A/VSJ310Q.Seq.d/                         84   3e-15
SFE342 (SFE342Q) /CSM/SF/SFE3-B/SFE342Q.Seq.d/                         50   4e-05
AFH112 (AFH112Q) /CSM/AF/AFH1-A/AFH112Q.Seq.d/                         50   4e-05
SFE463 (SFE463Q) /CSM/SF/SFE4-C/SFE463Q.Seq.d/                         48   2e-04
CFC648 (CFC648Q) /CSM/CF/CFC6-B/CFC648Q.Seq.d/                         48   2e-04

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    50  0.013  5
AX489505|AX489505.1 Sequence 6805 from Patent WO02053728.         50  0.034  1
AC005139|AC005139.3 Plasmodium falciparum chromosome 12, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    48  0.048  3
AC016179|AC016179.6 Homo sapiens chromosome 15 clone RP11-652K13 map 15, WORKING DRAFT SEQUENCE, 40 unordered pieces.    44  0.13  2
BU496172|BU496172.1 PfESToac04g05.y1 Plasmodium falciparum 3D7 asexual cDNA Plasmodium falciparum cDNA 5' similar to TR:Q61402 Q61402 GRANULE CELL ANTISERUM POSITIVE 8 ;, mRNA sequence.    48  0.13  1
AL922813|AL922813.1 Danio rerio EST sequence, clone 116-E11-2.    48  0.13  1
Z50796|Z50796.2 Caenorhabditis elegans cosmid T05A6.              40  0.29  2
AC135398|AC135398.1 Oryza sativa (japonica cultivar-group) chromosome 11 clone OSJNBa0079N13, *** SEQUENCING IN PROGRESS ***, 7 ordered pieces.    40  0.32  2
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    48  0.49  5
AF019985|AF019985.1 Dictyostelium discoideum Spalten (spnA) mRNA, complete cds.    44  0.51  2

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 410,164,479

 psg: 0.54  gvh: 0.53  alm: 0.49  top: 0.53  tms: 0.00  mit: 0.59  mip: 0.15
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 88.0 %: mitochondrial
	  8.0 %: nuclear
	  4.0 %: cytoplasmic

>> prediction for AFO328 is mit

>AFO328F.Seq
CACTGTTGGCCTACTGGAATTATAACCTTTCACATGATATAGTCGACAATATTTGAAGTC
ATTCAAATAATTTTTAATAAACAAAAATAAAATTTAATAAAATAAAATTTATAGAAAACA
ATATAAAATAAAAACAAAAAACAAAGTAATAAAAATATTAAAAAAAAAA----------

>AFO328Z.Seq
----------GNNNNGNNNNNNNTNNNNNTTNNTNNNTTNNTNNNNATAGTNNTNATNNA
GTTNTTNNNNNNNNTATTNNNNTGGTTATTANNNATNNNNCNNTTNANAGTNNAAANAGT
NANNATTCATTATTTAATATTGANCNTTCNNTTGGTATTGTTNNNNCAATGNTTAGTANA
NNTGNTNCNTTAAGANCTANAGNCAGTAGATCNAATAGAAATNCAATGGATCNTGAATTA
TTCTCTTCACTTCAACCATTAACTCCAACTTCAAAACATACTACTACCNCTGCAACTAAT
ACAAATACAAATACAAACACATGAATTAGATTCATTATTGAAACCAATAGCTGCATTATA
TGATAGAGCAATTAAATGGAAATCTGAATCAAATTCATCCTCATCTTCATCAGCATTAAA
TCAATCAAATGGTAGTGGTAATGAACAAGTTGTTGCTGCACCTTCTGTT

>AFO328P.Seq
CACTGTTGGCCTACTGGAATTATAACCTTTCACATGATATAGTCGACAATATTTGAAGTC
ATTCAAATAATTTTTAATAAACAAAAATAAAATTTAATAAAATAAAATTTATAGAAAACA
ATATAAAATAAAAACAAAAAACAAAGTAATAAAAATATTAAAAAAAAAA----------G
NNNNGNNNNNNNTNNNNNTTNNTNNNTTNNTNNNNATAGTNNTNATNNAGTTNTTNNNNN
NNNTATTNNNNTGGTTATTANNNATNNNNCNNTTNANAGTNNAAANAGTNANNATTCATT
ATTTAATATTGANCNTTCNNTTGGTATTGTTNNNNCAATGNTTAGTANANNTGNTNCNTT
AAGANCTANAGNCAGTAGATCNAATAGAAATNCAATGGATCNTGAATTATTCTCTTCACT
TCAACCATTAACTCCAACTTCAAAACATACTACTACCNCTGCAACTAATACAAATACAAA
TACAAACACATGAATTAGATTCATTATTGAAACCAATAGCTGCATTATATGATAGAGCAA
TTAAATGGAAATCTGAATCAAATTCATCCTCATCTTCATCAGCATTAAATCAATCAAATG
GTAGTGGTAATGAACAAGTTGTTGCTGCACCTTCTGTT