NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFB673
Library AF
(Link to library)
Clone ID AFB673
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16252-1
Original site URL
Representative seq. ID AFB673P
(Link to Original site)
Representative DNA sequence 
>AFB673 (AFB673Q) /CSM/AF/AFB6-D/AFB673Q.Seq.d/
ACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAA
ATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCT
TTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATC
AACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAA
AGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAA
ATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAA
TAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGG
TGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAAT
GAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATT
AGCTATGCAAGAATTTATGATTTTACCAXXXXXXXXXXGTCAAGATGCTATCAATGTTGG
TGATGAAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAA
ACTTGCCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGC
CTCTGAATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGG
TTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATA
TCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATT
AACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAG
AATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAAT
TGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGT
CATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGG
TTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATA
CAATCAACTTGTTAGAATTAACGAAGAGTTAGGTAAAACCATAACTATGCGGTTNACTTT
CCGTAA
sequence update 2001. 6. 1
Translated Amino Acid sequence 
LYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGAS
TGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPN
KGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKL
AMQEFMILP---

---QDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASEFKVENGYD
LDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTASVDIQIVG
DDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETE
DTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGKTITMRXTFR
Translated Amino Acid sequence (All Frames) 
Frame A:
tlfffisiyiyiif*nerhqin*sqrnfrftw*pnc*s*plhrkrwccfiiqscssirci
nwyl*sc*ikrw**ikilr*rctqsnqkhsrshstsrhw*isfrsscyr*indrsrwypk
*r*ircqrypcsqfsclqswcc**kfttlqiylrncry*neitsssiqcyqwwftcr**i
syariydft---

---vkmlsmlvmkvvslhqfkatkkvlns*nlplkklvtqv*lklvwivlplnsklkmvt
iltsklktmtvqllslvkn*vistenslkniqsfqlkihlikmignhtln*qhqlifkll
vmiyllptqkelrlvlkrkhvmlfy*kltklvqslnqfvplltqrmhhgvswlaivqvkl
kipslli*llv*vlvklklvlhvdqkd*pntinlleltks*vkp*lcgxlsv

Frame B:
LYFFLSLSIYISFFKMSVIKSIKAREILDSRGNPTVEVDLYTEKDGVVSSFRAAVPSGAS
TGIYEAVELRDGDKSRYLGKGVLKAIKNILEVIQPAVIGKSVSDQAAIDKLMIDLDGTPN
KGKLGANAILAVSLAVCRAGAADRNLPLYKYISEIAGTKMRLPVPAFNVINGGSHAGNKL
AMQEFMILP---

---srcyqcw**rwfrstnskqqrrs*tlktch*kswlhrfs*nwyglccl*iqs*kwlr
s*lqn*kq*rfscylw*kir*slqrih*risnhfn*rsi*sr*lgiih*insis*ysncw
**ftcyqprkn*dwy*kesm*csfiks*pnwfsh*inscrs*lkecimgchg*psfr*n*
rylhc*fscwfrywsn*nwcsm*irkisqiqstc*n*rrvr*nhnyavxfp*

Frame C:
fiffylylyiyhflk*assnqlkpekf*ihvvtqllkltftpkkmvlfhhselqfhqvhq
lvfmklln*emvinqdt*vkvyskqsktfsksfnqpslvnqfpikllsin**sismvpqi
kvn*vptlslqsv*lfaelvllieiyhftnisqklqvlk*dyqfqhsmlsmvvhmqvin*
lcknl*fy---

---QDAINVGDEGGFAPPIQSNKEGLELLKLAIEKAGYTGLVKIGMDCAASEFKVENGYD
LDFKTKNNDGSAVISGEKLGDLYREFIKEYPIISIEDPFDQDDWESYTKLTASVDIQIVG
DDLLVTNPERIKTGIEKKACNALLLKVNQIGSVTESIRAALDSKNASWGVMVSHRSGETE
DTFIADLVVGLGTGQIKTGAPCRSERLAKYNQLVRINEELGKTITMRXTFR
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFB673 (AFB673Q) /CSM/AF/AFB6-D/AFB673Q.Seq.d/                       2415   0.0
SFG643 (SFG643Q) /CSM/SF/SFG6-B/SFG643Q.Seq.d/                       1344   0.0
AFJ620 (AFJ620Q) /CSM/AF/AFJ6-A/AFJ620Q.Seq.d/                       1342   0.0
AFO841 (AFO841Q) /CSM/AF/AFO8-B/AFO841Q.Seq.d/                       1338   0.0
SFH339 (SFH339Q) /CSM/SF/SFH3-B/SFH339Q.Seq.d/                       1330   0.0
AFN685 (AFN685Q) /CSM/AF/AFN6-D/AFN685Q.Seq.d/                       1330   0.0
AFC510 (AFC510Q) /CSM/AF/AFC5-A/AFC510Q.Seq.d/                       1322   0.0
VFE233 (VFE233Q) /CSM/VF/VFE2-B/VFE233Q.Seq.d/                       1318   0.0
SSI448 (SSI448Q) /CSM/SS/SSI4-B/SSI448Q.Seq.d/                       1318   0.0
SFE744 (SFE744Q) /CSM/SF/SFE7-B/SFE744Q.Seq.d/                       1318   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X80474|X80474.1 N.frontalis enolase gene.                         64  3e-74  12
Y17298|Y17298.1 Cunninghamella elegans mRNA for enolase.          86  1e-72  11
BD183626|BD183626.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76  4e-61  10
BD183625|BD183625.1 Material for diagnosing malaria and immune antigen for suppressing growth of plasmodium.    76  4e-61  10
AB026051|AB026051.1 Plasmodium falciparum mRNA for enolase, complete cds.    76  4e-61  10
U00152|U00152.1 Plasmodium falciparum enolase gene complete cds.    76  4e-59  9
BM165201|BM165201.1 EST567724 PyBS Plasmodium yoelii yoelii cDNA clone PYCMI87 5' end, mRNA sequence.    92  2e-52  5
BM395755|BM395755.1 5009-0-10-H08.t.1 Chilcoat/Turkewitz cDNA (large fraction) Tetrahymena thermophila cDNA, mRNA sequence.   121  8e-49  5
AF348930|AF348930.1 Tetrahymena bergeri enolase (eno) gene, partial cds.    66  2e-46  6
AX619982|AX619982.1 Sequence 2945 from Patent WO02094868.         50  5e-46  10
dna update 2003. 7.24
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54RK5) RecName: Full=Enolase A;          EC=4.2.1.11; AltName:...   748   0.0
EF137905_1(EF137905|pid:none) Helianthus annuus plastid enolase ...   555   e-156
AC016662_10(AC016662|pid:none) Arabidopsis thaliana chromosome 1...   545   e-153
AK243111_1(AK243111|pid:none) Oryza sativa Japonica Group cDNA, ...   535   e-150
EU964132_1(EU964132|pid:none) Zea mays clone 276124 enolase mRNA...   533   e-150
DQ497988_1(DQ497988|pid:none) Polypterus senegalus enolase B mRN...   533   e-150
BC096516_1(BC096516|pid:none) Xenopus tropicalis hypothetical pr...   532   e-150
BC045082_1(BC045082|pid:none) Xenopus laevis enolase 3, beta mus...   531   e-149
(P26300) RecName: Full=Enolase;          EC=4.2.1.11; AltName: F...   530   e-149
T12341(T12341) phosphopyruvate hydratase (EC 4.2.1.11) - common ...   529   e-149
protein update 2009. 3.27
PSORT 

 psg: 0.93  gvh: 0.46  alm: 0.33  top: 0.60  tms: 0.00  mit: 0.34  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for AFB673 is cyt
5' end seq. ID AFB673F
5' end seq. 
>AFB673F.Seq
ACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAA
ATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCT
TTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATC
AACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAA
AGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAA
ATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAA
TAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGG
TGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAAT
GAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATT
AGCTATGCAAGAATTTATGATTTTACCA----------
Length of 5' end seq. 568
3' end seq. ID AFB673Z
3' end seq. 
>AFB673Z.Seq
----------GTCAAGATGCTATCAATGTTGGTGATGAAGGTGGTTTCGCTCCACCAATT
CAAAGCAACAAAGAAGGTCTTGAACTCTTAAAACTTGCCATTGAAAAAGCTGGTTACACA
GGTTTAGTTAAAATTGGTATGGATTGTGCTGCCTCTGAATTCAAAGTTGAAAATGGTTAC
GATCTTGACTTCAAAACTAAAAACAATGACGGTTCAGCTGTTATCTCTGGTGAAAAATTA
GGTGATCTCTACAGAGAATTCATTAAAGAATATCCAATCATTTCAATTGAAGATCCATTT
GATCAAGATGATTGGGAATCATACACTAAATTAACAGCATCAGTTGATATTCAAATTGTT
GGTGATGATTTACTTGTTACCAACCCAGAAAGAATTAAGACTGGTATTGAAAAGAAAGCA
TGTAATGCTCTTTTATTAAAAGTTAACCAAATTGGTTCAGTCACTGAATCAATTCGTGCC
GCTCTTGACTCAAAGAATGCATCATGGGGTGTCATGGTTAGCCATCGTTCAGGTGAAACT
GAAGATACCTTCATTGCTGATTTAGTTGTTGGTTTAGGTACTGGTCAAATTAAAACTGGT
GCTCCATGTAGATCAGAAAGATTAGCCAAATACAATCAACTTGTTAGAATTAACGAAGAG
TTAGGTAAAACCATAACTATGCGGTTNACTTTCCGTAA
Length of 3' end seq. 688
Connected seq. ID AFB673P
Connected seq. 
>AFB673P.Seq
ACTTTATTTTTTTTTATCTCTATCTATATATATATCATTTTTTAAAATGAGCGTCATCAA
ATCAATTAAAGCCAGAGAAATTTTAGATTCACGTGGTAACCCAACTGTTGAAGTTGACCT
TTACACCGAAAAAGATGGTGTTGTTTCATCATTCAGAGCTGCAGTTCCATCAGGTGCATC
AACTGGTATTTATGAAGCTGTTGAATTAAGAGATGGTGATAAATCAAGATACTTAGGTAA
AGGTGTACTCAAAGCAATCAAAAACATTCTCGAAGTCATTCAACCAGCCGTCATTGGTAA
ATCAGTTTCCGATCAAGCTGCTATCGATAAATTAATGATCGATCTCGATGGTACCCCAAA
TAAAGGTAAATTAGGTGCCAACGCTATCCTTGCAGTCAGTTTAGCTGTTTGCAGAGCTGG
TGCTGCTGATAGAAATTTACCACTTTACAAATATATCTCAGAAATTGCAGGTACTAAAAT
GAGATTACCAGTTCCAGCATTCAATGTTATCAATGGTGGTTCACATGCAGGTAATAAATT
AGCTATGCAAGAATTTATGATTTTACCA----------GTCAAGATGCTATCAATGTTGG
TGATGAAGGTGGTTTCGCTCCACCAATTCAAAGCAACAAAGAAGGTCTTGAACTCTTAAA
ACTTGCCATTGAAAAAGCTGGTTACACAGGTTTAGTTAAAATTGGTATGGATTGTGCTGC
CTCTGAATTCAAAGTTGAAAATGGTTACGATCTTGACTTCAAAACTAAAAACAATGACGG
TTCAGCTGTTATCTCTGGTGAAAAATTAGGTGATCTCTACAGAGAATTCATTAAAGAATA
TCCAATCATTTCAATTGAAGATCCATTTGATCAAGATGATTGGGAATCATACACTAAATT
AACAGCATCAGTTGATATTCAAATTGTTGGTGATGATTTACTTGTTACCAACCCAGAAAG
AATTAAGACTGGTATTGAAAAGAAAGCATGTAATGCTCTTTTATTAAAAGTTAACCAAAT
TGGTTCAGTCACTGAATCAATTCGTGCCGCTCTTGACTCAAAGAATGCATCATGGGGTGT
CATGGTTAGCCATCGTTCAGGTGAAACTGAAGATACCTTCATTGCTGATTTAGTTGTTGG
TTTAGGTACTGGTCAAATTAAAACTGGTGCTCCATGTAGATCAGAAAGATTAGCCAAATA
CAATCAACTTGTTAGAATTAACGAAGAGTTAGGTAAAACCATAACTATGCGGTTNACTTT
CCGTAA
Length of connected seq. 1256
Full length Seq ID -
Full length Seq. -
Length of full length seq. -