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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHJ195
Library VH
(Link to library)
Clone ID VHJ195
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11049-1
Original site URL
Representative seq. ID VHJ195P
(Link to Original site)
Representative DNA sequence 
>VHJ195 (VHJ195Q) /CSM/VH/VHJ1-D/VHJ195Q.Seq.d/
AAAACATAAAGACACATTAAAACAAACAAATAATATTTAATAATGTCACTTGTTGTATCT
TTATCATCACCAACAGCAGAAGAATGGTCAAATGAAAATTCAAGAGAAATTCATTACACC
AACGGATCTTTAGGTTGGAGTGAATGTAATCGTTCAGTCGAACCAATTGGTGAAGAGTTT
GAATTAGTTTGGAGTAAAGTCAAAAAGGGTGATGTCGAAATCATTGGCTTGGTTAGTGCC
ACTTCAGATGACAAGAAATCTCAAGATTCAAATGATGATGACAACATTGACGAATTAAAA
GAAAACTATAAAAATAATCATAAAGAGTGGACTAAAACTGGTAAAGATGTTAATCATTAC
AAAATCATGGGTTTAGATCATTTACGTTGGAGAGCTACCGATAATGATATTAAATTAGCA
TATAAAAAAATGATTTTAGTTGTTCATCCAGATAAAAATCAAGATTTAGGTGGTAATGAT
GAAGCATTTAAAGCATTAGTTAAATCAAATAATATTTTAAGTGATATTAAAAAGAGAAGA
GCATACGATTCAACCGATCCATTTGATGATGATATTCCAACAGCCGATGAXXXXXXXXXX
GCGAGCTCACCAAAGAAATGGAAACCAAAAATAATGTCACCGATAAGAAAGCAGTTTTCC
TCGTCCAATTGAACGAAATTCAAGAGAAACAAAGAGAGGCCGACAGACTCTATCAAGAGC
AAAGAAAAGCATTACTCAACAATAAGAAAGAAGCTAAACCAGAGAGACCATGGACTGAAG
ATGAACTCCATCTCTTGGCTAAAGCCATCCAAAAGTTCCCACCAGGTGTTACCAAACGTT
GGGAATCCGTTGCATCTTGTATTCCAACTCGTTCTTTGAAAGACGTTATCGCCAAAGCTA
AAACCTCACAACCAACCCAACAACAATCTTTCTCAAAACCAGAAGCTGCTCCATCAGGTT
CCGATTATGATAAATTAAAAGCCAAAGTTGGTCATATGGAAATTAAATCTGAACTCAGTT
CAAAAGTTGACCTCGGTCAATATCCAGTAGGTACCACTTCAAATGGCACTGAATCAACCA
CTACCGCCACCGCTCCATCAACTGTCGACACCGCCAAACCAGCCGCTAAAAAAGAAACTA
CCACTACCACTGCCTCTGTTGAATGGACACCAGAAGAACAAAAACTCTTGGAAGAAGCTT
TACAAAAAGTCGATAAAAATGCTGAAGATCGTTGGGATCAAATCGCAGCTCGTCTCGGTA
CTAAATCTAAAAAAGATTGTGNTGNNNGTAAACAAAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
kt*rhiktnk*YLIMSLVVSLSSPTAEEWSNENSREIHYTNGSLGWSECNRSVEPIGEEF
ELVWSKVKKGDVEIIGLVSATSDDKKSQDSNDDDNIDELKENYKNNHKEWTKTGKDVNHY
KIMGLDHLRWRATDNDIKLAYKKMILVVHPDKNQDLGGNDEAFKALVKSNNILSDIKKRR
AYDSTDPFDDDIPTAD---

---ELTKEMETKNNVTDKKAVFLVQLNEIQEKQREADRLYQEQRKALLNNKKEAKPERPW
TEDELHLLAKAIQKFPPGVTKRWESVASCIPTRSLKDVIAKAKTSQPTQQQSFSKPEAAP
SGSDYDKLKAKVGHMEIKSELSSKVDLGQYPVGTTSNGTESTTTATAPSTVDTAKPAAKK
ETTTTTASVEWTPEEQKLLEEALQKVDKNAEDRWDQIAARLGTKSKKDCXXXKQN
Translated Amino Acid sequence (All Frames) 
Frame A:
kt*rhiktnk*YLIMSLVVSLSSPTAEEWSNENSREIHYTNGSLGWSECNRSVEPIGEEF
ELVWSKVKKGDVEIIGLVSATSDDKKSQDSNDDDNIDELKENYKNNHKEWTKTGKDVNHY
KIMGLDHLRWRATDNDIKLAYKKMILVVHPDKNQDLGGNDEAFKALVKSNNILSDIKKRR
AYDSTDPFDDDIPTAD---

---asspkkwkpkimspirkqfsssn*tkfkrnkerptdsikskekhystirkklnqrdh
glkmnsiswlkpsksshqvlpnvgnplhlvfqlvl*ktlspklkphnqpnnnlsqnqkll
hqvpimin*kpklviwklnlnsvqkltsvniq*vplqmalnqplppplhqlstppnqplk
kklplplpllnghqknknswkklykksikmlkivgiksqlvsvlnlkkivxxvnki

Frame B:
khkdtlkqtnni**chllylyhhqqqkngqmkiqekfitptdl*vgvnvivqsnqlvksl
n*fgvkskrvmskslawlvplqmtrnlkiqmmmttltn*kktikiiiksglklvkmliit
kswv*iiyvgelpimiln*hikk*f*lfiqikiki*vvmmkhlkh*lnqiif*vilkree
htiqpihlmmifqqpm---

---rahqrngnqk*chr*essfprpiernsretkrgrqtlsrakksitqq*ers*tretm
d*r*tpslg*shpkvptrcyqtlgircilysnsfferryrqs*nlttnpttiflktrscs
irfrl**iksqswsygn*i*tqfks*prsissryhfkwh*inhyrhrsincrhrqtsr*k
rnyhyhclc*mdtrrtktlgrsftksr*kc*rslgsnrsssry*i*krlxxx*tk

Frame C:
nikth*nkqiifnnvtccifiitnsrrmvk*kfkrnslhqrifrle*m*sfsrtnw*rv*
isle*sqkg*crnhwlg*chfr*qeisrfk***qh*rikrkl*k*s*rvd*nw*rc*slq
nhgfrsftlesyr**y*isi*kndfscssr*ksrfrw***si*sis*ik*yfk*y*keks
irfnrsi***ysnsr*---

---ELTKEMETKNNVTDKKAVFLVQLNEIQEKQREADRLYQEQRKALLNNKKEAKPERPW
TEDELHLLAKAIQKFPPGVTKRWESVASCIPTRSLKDVIAKAKTSQPTQQQSFSKPEAAP
SGSDYDKLKAKVGHMEIKSELSSKVDLGQYPVGTTSNGTESTTTATAPSTVDTAKPAAKK
ETTTTTASVEWTPEEQKLLEEALQKVDKNAEDRWDQIAARLGTKSKKDCXXXKQN
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHJ195 (VHJ195Q) /CSM/VH/VHJ1-D/VHJ195Q.Seq.d/                       2450   0.0
AFN547 (AFN547Q) /CSM/AF/AFN5-B/AFN547Q.Seq.d/                       1342   0.0
SLE172 (SLE172Q) /CSM/SL/SLE1-C/SLE172Q.Seq.d/                       1102   0.0
SLD492 (SLD492Q) /CSM/SL/SLD4-D/SLD492Q.Seq.d/                        815   0.0
SLD569 (SLD569Q) /CSM/SL/SLD5-C/SLD569Q.Seq.d/                        583   e-165
SLA508 (SLA508Q) /CSM/SL/SLA5-A/SLA508Q.Seq.d/                        367   e-100
SSF704 (SSF704Q) /CSM/SS/SSF7-A/SSF704Q.Seq.d/                         42   0.021
AFC244 (AFC244Q) /CSM/AF/AFC2-B/AFC244Q.Seq.d/                         42   0.021
VSK435 (VSK435Q) /CSM/VS/VSK4-B/VSK435Q.Seq.d/                         40   0.083
VFI629 (VFI629Q) /CSM/VF/VFI6-B/VFI629Q.Seq.d/                         40   0.083
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442455) Dictyostelium discoideum cDNA clone:ddv49m23, 3' ...  1360   0.0    1
(BJ345667) Dictyostelium discoideum cDNA clone:dda28m11, 3' ...  1340   0.0    1
(AU034677) Dictyostelium discoideum slug cDNA, clone SLE172.     1102   0.0    1
(BJ423645) Dictyostelium discoideum cDNA clone:ddv49m23, 5' ...   839   0.0    2
(BJ328502) Dictyostelium discoideum cDNA clone:dda28m11, 5' ...   839   0.0    2
(AU052538) Dictyostelium discoideum slug cDNA, clone SLD492.      815   0.0    1
(AU061226) Dictyostelium discoideum slug cDNA, clone SLD569.      337   e-164  2
(C83939) Dictyostelium discoideum slug cDNA, clone SLA508.        359   2e-94  1
(AU052597) Dictyostelium discoideum slug cDNA, clone SLD569.      113   2e-20  1
(AU061182) Dictyostelium discoideum slug cDNA, clone SLD492.       60   2e-04  1
dna update 2008.11.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

BC067568_1(BC067568|pid:none) Danio rerio DnaJ (Hsp40) homolog, ...    78   6e-13
BC068855_1(BC068855|pid:none) Xenopus laevis hypothetical protei...    78   8e-13
AE014296_3674(AE014296|pid:none) Drosophila melanogaster chromos...    77   1e-12
BC062703_1(BC062703|pid:none) Homo sapiens DnaJ (Hsp40) homolog,...    75   5e-12
AF118853_1(AF118853|pid:none) Rattus norvegicus gliosarcoma-rela...    75   5e-12
(Q1RMH9) RecName: Full=DnaJ homolog subfamily C member 2; AltNam...    75   5e-12
(Q99543) RecName: Full=DnaJ homolog subfamily C member 2; AltNam...    75   5e-12
AY322162_1(AY322162|pid:none) Rattus norvegicus zuotin related f...    75   5e-12
BC139751_1(BC139751|pid:none) Homo sapiens DnaJ (Hsp40) homolog,...    75   5e-12
(Q7TQ20) RecName: Full=DnaJ homolog subfamily C member 2; AltNam...    75   5e-12
protein update 2009. 7.14
PSORT 

 psg: 0.95  gvh: 0.59  alm: 0.47  top: 0.30  tms: 0.00  mit: 0.31  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 36.0 %: extracellular, including cell wall
	 28.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: Golgi
	  4.0 %: mitochondrial
	  4.0 %: endoplasmic reticulum

>> prediction for VHJ195 is exc
5' end seq. ID VHJ195F
5' end seq. 
>VHJ195F.Seq
AAAACATAAAGACACATTAAAACAAACAAATAATATTTAATAATGTCACTTGTTGTATCT
TTATCATCACCAACAGCAGAAGAATGGTCAAATGAAAATTCAAGAGAAATTCATTACACC
AACGGATCTTTAGGTTGGAGTGAATGTAATCGTTCAGTCGAACCAATTGGTGAAGAGTTT
GAATTAGTTTGGAGTAAAGTCAAAAAGGGTGATGTCGAAATCATTGGCTTGGTTAGTGCC
ACTTCAGATGACAAGAAATCTCAAGATTCAAATGATGATGACAACATTGACGAATTAAAA
GAAAACTATAAAAATAATCATAAAGAGTGGACTAAAACTGGTAAAGATGTTAATCATTAC
AAAATCATGGGTTTAGATCATTTACGTTGGAGAGCTACCGATAATGATATTAAATTAGCA
TATAAAAAAATGATTTTAGTTGTTCATCCAGATAAAAATCAAGATTTAGGTGGTAATGAT
GAAGCATTTAAAGCATTAGTTAAATCAAATAATATTTTAAGTGATATTAAAAAGAGAAGA
GCATACGATTCAACCGATCCATTTGATGATGATATTCCAACAGCCGATGANNNNNNNNNN
Length of 5' end seq. 600
3' end seq. ID VHJ195Z
3' end seq. 
>VHJ195Z.Seq
NNNNNNNNNNGCGAGCTCACCAAAGAAATGGAAACCAAAAATAATGTCACCGATAAGAAA
GCAGTTTTCCTCGTCCAATTGAACGAAATTCAAGAGAAACAAAGAGAGGCCGACAGACTC
TATCAAGAGCAAAGAAAAGCATTACTCAACAATAAGAAAGAAGCTAAACCAGAGAGACCA
TGGACTGAAGATGAACTCCATCTCTTGGCTAAAGCCATCCAAAAGTTCCCACCAGGTGTT
ACCAAACGTTGGGAATCCGTTGCATCTTGTATTCCAACTCGTTCTTTGAAAGACGTTATC
GCCAAAGCTAAAACCTCACAACCAACCCAACAACAATCTTTCTCAAAACCAGAAGCTGCT
CCATCAGGTTCCGATTATGATAAATTAAAAGCCAAAGTTGGTCATATGGAAATTAAATCT
GAACTCAGTTCAAAAGTTGACCTCGGTCAATATCCAGTAGGTACCACTTCAAATGGCACT
GAATCAACCACTACCGCCACCGCTCCATCAACTGTCGACACCGCCAAACCAGCCGCTAAA
AAAGAAACTACCACTACCACTGCCTCTGTTGAATGGACACCAGAAGAACAAAAACTCTTG
GAAGAAGCTTTACAAAAAGTCGATAAAAATGCTGAAGATCGTTGGGATCAAATCGCAGCT
CGTCTCGGTACTAAATCTAAAAAAGATTGTGNTGNNNGTAAACAAAATA
Length of 3' end seq. 709
Connected seq. ID VHJ195P
Connected seq. 
>VHJ195P.Seq
AAAACATAAAGACACATTAAAACAAACAAATAATATTTAATAATGTCACTTGTTGTATCT
TTATCATCACCAACAGCAGAAGAATGGTCAAATGAAAATTCAAGAGAAATTCATTACACC
AACGGATCTTTAGGTTGGAGTGAATGTAATCGTTCAGTCGAACCAATTGGTGAAGAGTTT
GAATTAGTTTGGAGTAAAGTCAAAAAGGGTGATGTCGAAATCATTGGCTTGGTTAGTGCC
ACTTCAGATGACAAGAAATCTCAAGATTCAAATGATGATGACAACATTGACGAATTAAAA
GAAAACTATAAAAATAATCATAAAGAGTGGACTAAAACTGGTAAAGATGTTAATCATTAC
AAAATCATGGGTTTAGATCATTTACGTTGGAGAGCTACCGATAATGATATTAAATTAGCA
TATAAAAAAATGATTTTAGTTGTTCATCCAGATAAAAATCAAGATTTAGGTGGTAATGAT
GAAGCATTTAAAGCATTAGTTAAATCAAATAATATTTTAAGTGATATTAAAAAGAGAAGA
GCATACGATTCAACCGATCCATTTGATGATGATATTCCAACAGCCGATGA----------
GCGAGCTCACCAAAGAAATGGAAACCAAAAATAATGTCACCGATAAGAAAGCAGTTTTCC
TCGTCCAATTGAACGAAATTCAAGAGAAACAAAGAGAGGCCGACAGACTCTATCAAGAGC
AAAGAAAAGCATTACTCAACAATAAGAAAGAAGCTAAACCAGAGAGACCATGGACTGAAG
ATGAACTCCATCTCTTGGCTAAAGCCATCCAAAAGTTCCCACCAGGTGTTACCAAACGTT
GGGAATCCGTTGCATCTTGTATTCCAACTCGTTCTTTGAAAGACGTTATCGCCAAAGCTA
AAACCTCACAACCAACCCAACAACAATCTTTCTCAAAACCAGAAGCTGCTCCATCAGGTT
CCGATTATGATAAATTAAAAGCCAAAGTTGGTCATATGGAAATTAAATCTGAACTCAGTT
CAAAAGTTGACCTCGGTCAATATCCAGTAGGTACCACTTCAAATGGCACTGAATCAACCA
CTACCGCCACCGCTCCATCAACTGTCGACACCGCCAAACCAGCCGCTAAAAAAGAAACTA
CCACTACCACTGCCTCTGTTGAATGGACACCAGAAGAACAAAAACTCTTGGAAGAAGCTT
TACAAAAAGTCGATAAAAATGCTGAAGATCGTTGGGATCAAATCGCAGCTCGTCTCGGTA
CTAAATCTAAAAAAGATTGTGNTGNNNGTAAACAAAATA
Length of connected seq. 1289
Full length Seq ID -
Full length Seq. -
Length of full length seq. -