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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHJ190
Library VH
(Link to library)
Clone ID VHJ190
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16490-1
Original site URL
Representative seq. ID VHJ190P
(Link to Original site)
Representative DNA sequence 
>VHJ190 (VHJ190Q) /CSM/VH/VHJ1-D/VHJ190Q.Seq.d/
ATTTTTTCAAAAAAATTACATTTCAATAATTCAAATTCATATTAAAATGTTTGGTTTTAT
ATATAGAGACCCTTCTCCAGCACCTCAAGGTAAAATTAGAGATGGTTCAAAAGACCCAAA
AACACCAGGTGGTGGTGGTGGTGGTGGTGGTGGTATTTCTCCAAATGGTGGTGCTCCCCT
TGGTGGTAAAGGATTCTCAATGTAGTTTTAACTTTATTTTGTTACAATTCGATAATTATT
ATTAAATCAAATAAAATTATTATATTTGGTTCATCTTCTATTTTAAAAAAAAAAXXXXXX
XXXXATTTTTTCAAAAAAATTACATTTCAATAATTCAAATTCATATTAAAATGTTTGGTT
TTATATATAGAGACCCTTCTCCAGCACCTCAAGGTAAAATTAGAGATGGTTCAAAAGACC
CAAAAACACCAGGTGGTGGTGGTGGTGGTGGTGGTGGTATTTCTCCAAATGGTGGTGCTC
CCCTTGGTGGTAAAGGATTCTCAATGTAGTTTTAACTTTATTTTGTTACAATTCGATAAT
TATTATTAAATCAAATAAAATTATTA
sequence update 2002.10.25
Translated Amino Acid sequence 
ifskklhfnnsnsy*nvwfyi*rpfsstsr*n*rwfkrpkntrwwwwwwwwyfskwwcsp
ww*RILNVVLTLFCYNSIIIIKSNKIIIFGSSSILKKK---

---FFQKNYISIIQIHIKMFGFIYRDPSPAPQGKIRDGSKDPKTPGGGGGGGGGISPNGG
APLGGKGFSM*f*lyfvtir*lllnqikll
Translated Amino Acid sequence (All Frames) 
Frame A:
ifskklhfnnsnsy*nvwfyi*rpfsstsr*n*rwfkrpkntrwwwwwwwwyfskwwcsp
ww*RILNVVLTLFCYNSIIIIKSNKIIIFGSSSILKKK---

---ifskklhfnnsnsy*nvwfyi*rpfsstsr*n*rwfkrpkntrwwwwwwwwyfskww
cspww*rilnvvltlfcynsiiiiksnkii

Frame B:
ffqknyisiiqihikmfgfiyrdpspapqgkirdgskdpktpgggggggggispnggapl
ggkgfsm*f*lyfvtir*lllnqikllylvhllf*kk---

---FFQKNYISIIQIHIKMFGFIYRDPSPAPQGKIRDGSKDPKTPGGGGGGGGGISPNGG
APLGGKGFSM*f*lyfvtir*lllnqikll

Frame C:
ffkkitfq*fkfilkclvlyietllqhlkvklemvqktqkhqvvvvvvvvvflqmvvlpl
vvkdsqcsfnfillqfdnyy*ik*nyyiwfifyfkkk---

---ffkkitfq*fkfilkclvlyietllqhlkvklemvqktqkhqvvvvvvvvvflqmvv
lplvvkdsqcsfnfillqfdnyy*ik*ny
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK358 (VSK358Q) /CSM/VS/VSK3-C/VSK358Q.Seq.d/                        258   8e-68
VSK330 (VSK330Q) /CSM/VS/VSK3-B/VSK330Q.Seq.d/                        258   8e-68
VSJ152 (VSJ152Q) /CSM/VS/VSJ1-C/VSJ152Q.Seq.d/                        258   8e-68
VSI808 (VSI808Q) /CSM/VS/VSI8-A/VSI808Q.Seq.d/                        258   8e-68
VSI734 (VSI734Q) /CSM/VS/VSI7-B/VSI734Q.Seq.d/                        258   8e-68
VSH249 (VSH249Q) /CSM/VS/VSH2-C/VSH249Q.Seq.d/                        258   8e-68
VSG802 (VSG802Q) /CSM/VS/VSG8-A/VSG802Q.Seq.d/                        258   8e-68
VSG716 (VSG716Q) /CSM/VS/VSG7-A/VSG716Q.Seq.d/                        258   8e-68
VSG430 (VSG430Q) /CSM/VS/VSG4-B/VSG430Q.Seq.d/                        258   8e-68
VSG252 (VSG252Q) /CSM/VS/VSG2-C/VSG252Q.Seq.d/                        258   8e-68
own update 2003. 1. 1
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AU265926) Dictyostelium discoideum vegetative cDNA clone:VS...   222   e-104  2
(BJ421858) Dictyostelium discoideum cDNA clone:ddv44g03, 5' ...   222   e-104  2
(BJ423598) Dictyostelium discoideum cDNA clone:ddv49c23, 5' ...   222   e-104  2
(BJ423484) Dictyostelium discoideum cDNA clone:ddv49i11, 5' ...   222   e-104  2
(AU265923) Dictyostelium discoideum vegetative cDNA clone:VS...   222   e-104  2
(AU284533) Dictyostelium discoideum gamete cDNA clone:FC-BB1...   222   e-104  2
(C24323) Dictyostelium discoideum gamete cDNA, clone FC-AQ17.     222   e-104  2
(BJ416769) Dictyostelium discoideum cDNA clone:ddv27p06, 5' ...   222   e-104  2
(AU266822) Dictyostelium discoideum vegetative cDNA clone:VS...   222   e-104  2
(AU269259) Dictyostelium discoideum vegetative cDNA clone:VS...   222   e-104  2
dna update 2008.11.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(B0G125) RecName: Full=Uncharacterized protein DDB_G0295473;           62   1e-08
protein update 2009. 3.12
PSORT 

 psg: 0.93  gvh: 0.42  alm: 0.26  top: 0.60  tms: 0.07  mit: 0.43  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: cytoplasmic
	 24.0 %: mitochondrial
	 24.0 %: nuclear
	  8.0 %: plasma membrane
	  8.0 %: endoplasmic reticulum
	  4.0 %: vesicles of secretory system
	  4.0 %: peroxisomal

>> prediction for VHJ190 is cyt
5' end seq. ID VHJ190F
5' end seq. 
>VHJ190F.Seq
ATTTTTTCAAAAAAATTACATTTCAATAATTCAAATTCATATTAAAATGTTTGGTTTTAT
ATATAGAGACCCTTCTCCAGCACCTCAAGGTAAAATTAGAGATGGTTCAAAAGACCCAAA
AACACCAGGTGGTGGTGGTGGTGGTGGTGGTGGTATTTCTCCAAATGGTGGTGCTCCCCT
TGGTGGTAAAGGATTCTCAATGTAGTTTTAACTTTATTTTGTTACAATTCGATAATTATT
ATTAAATCAAATAAAATTATTATATTTGGTTCATCTTCTATTTTAAAAAAAAAANNNNNN
NNNN
Length of 5' end seq. 304
3' end seq. ID VHJ190Z
3' end seq. 
>VHJ190Z.Seq
NNNNNNNNNNATTTTTTCAAAAAAATTACATTTCAATAATTCAAATTCATATTAAAATGT
TTGGTTTTATATATAGAGACCCTTCTCCAGCACCTCAAGGTAAAATTAGAGATGGTTCAA
AAGACCCAAAAACACCAGGTGGTGGTGGTGGTGGTGGTGGTGGTATTTCTCCAAATGGTG
GTGCTCCCCTTGGTGGTAAAGGATTCTCAATGTAGTTTTAACTTTATTTTGTTACAATTC
GATAATTATTATTAAATCAAATAAAATTATTA
Length of 3' end seq. 272
Connected seq. ID VHJ190P
Connected seq. 
>VHJ190P.Seq
ATTTTTTCAAAAAAATTACATTTCAATAATTCAAATTCATATTAAAATGTTTGGTTTTAT
ATATAGAGACCCTTCTCCAGCACCTCAAGGTAAAATTAGAGATGGTTCAAAAGACCCAAA
AACACCAGGTGGTGGTGGTGGTGGTGGTGGTGGTATTTCTCCAAATGGTGGTGCTCCCCT
TGGTGGTAAAGGATTCTCAATGTAGTTTTAACTTTATTTTGTTACAATTCGATAATTATT
ATTAAATCAAATAAAATTATTATATTTGGTTCATCTTCTATTTTAAAAAAAAAA------
----ATTTTTTCAAAAAAATTACATTTCAATAATTCAAATTCATATTAAAATGTTTGGTT
TTATATATAGAGACCCTTCTCCAGCACCTCAAGGTAAAATTAGAGATGGTTCAAAAGACC
CAAAAACACCAGGTGGTGGTGGTGGTGGTGGTGGTGGTATTTCTCCAAATGGTGGTGCTC
CCCTTGGTGGTAAAGGATTCTCAATGTAGTTTTAACTTTATTTTGTTACAATTCGATAAT
TATTATTAAATCAAATAAAATTATTA
Length of connected seq. 556
Full length Seq ID -
Full length Seq. -
Length of full length seq. -