>VHJ154 (VHJ154Q) /CSM/VH/VHJ1-C/VHJ154Q.Seq.d/
XXXXXXXXXXGTTATGTCATTGAATACTTTGGAGAATGATATGATCAATTTCTATAATCT
CCTCCCAACCTATTTAAAAGGCCCAAATAATCAATCGGATTGGATGATCAATACATTGGA
ACAAGCTCAATCCAATGGTGAAAAGGTTTTAATCATTGGTCATATTCCATGCACTGTTAA
AAGTGCTTCAACCGATGGTTGGTGTGCAATGTATGAACAAGTGGTAGGTCAATTCTCTGA
TGTTATCATTGATCAATTGTATGGCCATACTCATTACGATCAATTTTCAGTATTCTCCGA
TGTTGCAACTCATACAATTCCAACCGGTATGAATTATATCGTTCCATCATTAACAACCTA
TCAAAATCATGAACCTGGTTATCGTATTTATCAATTTGATTATTCAACTAATCAAATTGT
AAACTACTATCAATATCATGCAAACATTACTGAAGCAAATGAAACTGGTGCCTTAAATTT
CCAATTGACTTATAGTGCTAAAGAACTCTATAATATGGATGATCTTTCACCTACCTCTTG
GACAAAAGTTGCCAATCAAATGAAAACTAATTCAACAATGTTTAATAGTTATTTCGAAAA
TCTTAGTAGTTCACCAATTAAANAATACAANACNTCAAGCTTGTCAAA

---MSLNTLENDMINFYNLLPTYLKGPNNQSDWMINTLEQAQSNGEKVLIIGHIPCTVKS
ASTDGWCAMYEQVVGQFSDVIIDQLYGHTHYDQFSVFSDVATHTIPTGMNYIVPSLTTYQ
NHEPGYRIYQFDYSTNQIVNYYQYHANITEANETGALNFQLTYSAKELYNMDDLSPTSWT
KVANQMKTNSTMFNSYFENLSSSPIKXYXTSSLS

Frame A:
---lch*ilwrmi*sisiissqpi*kaqiinrig*sihwnklnpmvkrf*slvifhallk
vlqpmvgvqcmnkw*vnslmlslincmailitinfqyspmlqliqfqpv*iisfhh*qpi
kimnlvivfinliiqlikl*ttinimqtllkqmklvp*isn*livlknsiiwmifhlplg
qklpik*kliqqcliviskilvvhqlxntxxqacq


Frame B:
---yvieyfge*ydqfl*sppnlfkrpk*siglddqyigtssiqw*kgfnhwsysmhc*k
cfnrwlvcnv*tsgrsil*cyh*sivwpyslrsifsilrccnsynsnryelyrsiinnls
ks*twlsylsi*lfn*snckllsisckhy*sk*nwclkfpidl*c*rtl*yg*sftylld
kscqsnen*fnnv**lfrks**ftn*xiqxxklvk


Frame C:
---MSLNTLENDMINFYNLLPTYLKGPNNQSDWMINTLEQAQSNGEKVLIIGHIPCTVKS
ASTDGWCAMYEQVVGQFSDVIIDQLYGHTHYDQFSVFSDVATHTIPTGMNYIVPSLTTYQ
NHEPGYRIYQFDYSTNQIVNYYQYHANITEANETGALNFQLTYSAKELYNMDDLSPTSWT
KVANQMKTNSTMFNSYFENLSSSPIKXYXTSSLS

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHJ154 (VHJ154Q) /CSM/VH/VHJ1-C/VHJ154Q.Seq.d/                       1247   0.0
VHC519 (VHC519Q) /CSM/VH/VHC5-A/VHC519Q.Seq.d/                       1207   0.0
VHB601 (VHB601Q) /CSM/VH/VHB6-A/VHB601Q.Seq.d/                       1207   0.0
VFL234 (VFL234Q) /CSM/VF/VFL2-B/VFL234Q.Seq.d/                       1199   0.0
VHG223 (VHG223Q) /CSM/VH/VHG2-A/VHG223Q.Seq.d/                       1088   0.0
VFH473 (VFH473Q) /CSM/VF/VFH4-D/VFH473Q.Seq.d/                        997   0.0
SLK658 (SLK658Q) /CSM/SL/SLK6-C/SLK658Q.Seq.d/                        333   2e-90
VHG422 (VHG422Q) /CSM/VH/VHG4-A/VHG422Q.Seq.d/                        329   3e-89
SSI722 (SSI722Q) /CSM/SS/SSI7-A/SSI722Q.Seq.d/                         36   0.64
SLE618 (SLE618Q) /CSM/SL/SLE6-A/SLE618Q.Seq.d/                         36   0.64

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

BI514779|BI514779.1 BB160016A20G02.5 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160016A20G02 5', mRNA sequence.    46   0.61   1
AC012462|AC012462.5 Homo sapiens BAC clone RP11-566P21 from 2, complete sequence.    38   1.8    4
AP004824|AP004824.1 Staphylococcus aureus subsp. aureus MW2 DNA, complete genome, strain:MW2, section 3/10.    36   1.8    8
BX897714|BX897714.1 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-218L23.    38   2.0    8
AC111505|AC111505.5 Rattus norvegicus clone CH230-131N9, WORKING DRAFT SEQUENCE, 2 unordered pieces.    44   2.4    1
AC126851|AC126851.4 Rattus norvegicus clone CH230-22D14, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    44   2.4    1
BH546841|BH546841.1 BOGWT92TR BOGW Brassica oleracea genomic clone BOGWT92, DNA sequence.    44   2.4    1
AL161589|AL161589.2 Arabidopsis thaliana DNA chromosome 4, contig fragment No. 85.    32   4.2    2
Z99708|Z99708.1 Arabidopsis thaliana DNA chromosome 4, ESSA I AP2 contig fragment  No. 2.    32   4.2    2
AP001990|AP001990.2 Homo sapiens genomic DNA, chromosome 11q clone:RP11-790L8, WORKING DRAFT SEQUENCE, 11 unordered pieces.    36   5.9    6

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q23498) RecName: Full=Sphingomyelin phosphodiesterase 2;       ...   124   3e-27
(Q0VD19) RecName: Full=Sphingomyelin phosphodiesterase;         ...   124   3e-27
A39825(S27009;S36357;A39825;JX0276;S06957;I55567;A42801;) sphing...   123   5e-27
X63600_1(X63600|pid:none) H.sapiens genes for acid sphingomyelin...   123   5e-27
AK292388_1(AK292388|pid:none) Homo sapiens cDNA FLJ77600 complet...   123   5e-27
X59960_1(X59960|pid:none) H.sapiens mRNA for sphingomyelinase.        122   7e-27
(P17405) RecName: Full=Sphingomyelin phosphodiesterase;         ...   122   7e-27
M81780_2(M81780|pid:none) Homo sapiens acid sphingomyelinase (SM...   122   7e-27
BC011304_1(BC011304|pid:none) Mus musculus sphingomyelin phospho...   120   3e-26
AY261860_1(AY261860|pid:none) Ciona intestinalis sphingomyelin p...   120   3e-26

 psg: 0.75  gvh: 0.40  alm: 0.47  top: 0.53  tms: 0.00  mit: 0.23  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: endoplasmic reticulum

>> prediction for VHJ154 is cyt

>VHJ154Z.Seq
NNNNNNNNNNGTTATGTCATTGAATACTTTGGAGAATGATATGATCAATTTCTATAATCT
CCTCCCAACCTATTTAAAAGGCCCAAATAATCAATCGGATTGGATGATCAATACATTGGA
ACAAGCTCAATCCAATGGTGAAAAGGTTTTAATCATTGGTCATATTCCATGCACTGTTAA
AAGTGCTTCAACCGATGGTTGGTGTGCAATGTATGAACAAGTGGTAGGTCAATTCTCTGA
TGTTATCATTGATCAATTGTATGGCCATACTCATTACGATCAATTTTCAGTATTCTCCGA
TGTTGCAACTCATACAATTCCAACCGGTATGAATTATATCGTTCCATCATTAACAACCTA
TCAAAATCATGAACCTGGTTATCGTATTTATCAATTTGATTATTCAACTAATCAAATTGT
AAACTACTATCAATATCATGCAAACATTACTGAAGCAAATGAAACTGGTGCCTTAAATTT
CCAATTGACTTATAGTGCTAAAGAACTCTATAATATGGATGATCTTTCACCTACCTCTTG
GACAAAAGTTGCCAATCAAATGAAAACTAATTCAACAATGTTTAATAGTTATTTCGAAAA
TCTTAGTAGTTCACCAATTAAANAATACAANACNTCAAGCTTGTCAAA