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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHJ144
Library VH
(Link to library)
Clone ID VHJ144
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16490-1
Original site URL
Representative seq. ID VHJ144P
(Link to Original site)
Representative DNA sequence 
>VHJ144 (VHJ144Q) /CSM/VH/VHJ1-B/VHJ144Q.Seq.d/
ATATATATTCAATCTGAATCATTTTTTTTTATTTTTCATTTCAAAAAAATTACATTTCAA
TAATTCAAATTCATATTAAAATGTTTGGTTTTATATATAGAGACCCTTCTCCAGCACCTC
AAGGTAAAATTAGAGATGGTTCAAAAGACCCAAAAACACCAGGTGGTGGTGGTGGTGGTG
GTGGTGGTATTTCTCCAAATGGTGGTGCTCCCCTTGGTGGTAAAGGATTCTCAATGTAGT
TTTAACTTTATTTTGTTACAATTCGATAATTATTATTAAATCAAATAAAATTATTATATT
TGGTTCATCTTCTATTTTAAAAAAAAAAXXXXXXXXXXATATATATTCAATCTGAATCAT
TTTTTTTTATTTTTCATTTCAAAAAAATTACATTTCAATAATTCAAATTCATATTAAAAT
GTTTGGTTTTATATATAGAGACCCTTCTCCAGCACCTCAAGGTAAAATTAGAGATGGTTC
AAAAGACCCAAAAACACCAGGTGGTGGTGGTGGTGGTGGTGGTGGTATTTCTCCAAATGG
TGGTGCTCCCCTTGGTGGTAAAGGATTCTCAATGTAGTTTTAACTTTATTTTGTTACAAT
TCGATAATTATTATTAATCAAATAAAATTATTATATTGGGTTCATC
sequence update 2002.10.25
Translated Amino Acid sequence 
yifnlnhfflffiskklhfnnsnsy*nvwfyi*rpfsstsr*n*rwfkrpkntrwwwwww
wwyfskwwcspww*RILNVVLTLFCYNSIIIIKSNKIIIFGSSSILKKK---

---YIFNLNHFFLFFISKKLHFNNSNSY*nvwfyi*rpfsstsr*n*rwfkrpkntrwww
wwwwwyfskwwcspww*rilnvvltlfcynsiiiinqikllywvh
Translated Amino Acid sequence (All Frames) 
Frame A:
iyiqsesfffifhfkkitfq*fkfilkclvlyietllqhlkvklemvqktqkhqvvvvvv
vvvflqmvvlplvvkdsqcsfnfillqfdnyy*ik*nyyiwfifyfkkk---

---iyiqsesfffifhfkkitfq*fkfilkclvlyietllqhlkvklemvqktqkhqvvv
vvvvvvflqmvvlplvvkdsqcsfnfillqfdnyy*snkiiilgs

Frame B:
yifnlnhfflffiskklhfnnsnsy*nvwfyi*rpfsstsr*n*rwfkrpkntrwwwwww
wwyfskwwcspww*RILNVVLTLFCYNSIIIIKSNKIIIFGSSSILKKK---

---YIFNLNHFFLFFISKKLHFNNSNSY*nvwfyi*rpfsstsr*n*rwfkrpkntrwww
wwwwwyfskwwcspww*rilnvvltlfcynsiiiinqikllywvh

Frame C:
iysi*iiffyfsfqknyisiiqihikmfgfiyrdpspapqgkirdgskdpktpggggggg
ggispnggaplggkgfsm*f*lyfvtir*lllnqikllylvhllf*kk---

---iysi*iiffyfsfqknyisiiqihikmfgfiyrdpspapqgkirdgskdpktpgggg
gggggispnggaplggkgfsm*f*lyfvtir*lllik*nyyigfi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSK358 (VSK358Q) /CSM/VS/VSK3-C/VSK358Q.Seq.d/                        258   9e-68
VSK330 (VSK330Q) /CSM/VS/VSK3-B/VSK330Q.Seq.d/                        258   9e-68
VSJ152 (VSJ152Q) /CSM/VS/VSJ1-C/VSJ152Q.Seq.d/                        258   9e-68
VSI808 (VSI808Q) /CSM/VS/VSI8-A/VSI808Q.Seq.d/                        258   9e-68
VSI734 (VSI734Q) /CSM/VS/VSI7-B/VSI734Q.Seq.d/                        258   9e-68
VSH249 (VSH249Q) /CSM/VS/VSH2-C/VSH249Q.Seq.d/                        258   9e-68
VSG802 (VSG802Q) /CSM/VS/VSG8-A/VSG802Q.Seq.d/                        258   9e-68
VSG716 (VSG716Q) /CSM/VS/VSG7-A/VSG716Q.Seq.d/                        258   9e-68
VSG430 (VSG430Q) /CSM/VS/VSG4-B/VSG430Q.Seq.d/                        258   9e-68
VSG252 (VSG252Q) /CSM/VS/VSG2-C/VSG252Q.Seq.d/                        258   9e-68
own update 2003. 1. 1
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ423474) Dictyostelium discoideum cDNA clone:ddv49g11, 5' ...   222   e-110  3
(AU284533) Dictyostelium discoideum gamete cDNA clone:FC-BB1...   222   e-106  3
(C24323) Dictyostelium discoideum gamete cDNA, clone FC-AQ17.     222   e-106  3
(AU269159) Dictyostelium discoideum vegetative cDNA clone:VS...   222   e-106  3
(AU269259) Dictyostelium discoideum vegetative cDNA clone:VS...   222   e-105  3
(AU265926) Dictyostelium discoideum vegetative cDNA clone:VS...   222   e-104  2
(BJ421858) Dictyostelium discoideum cDNA clone:ddv44g03, 5' ...   222   e-104  2
(BJ423598) Dictyostelium discoideum cDNA clone:ddv49c23, 5' ...   222   e-104  2
(BJ423484) Dictyostelium discoideum cDNA clone:ddv49i11, 5' ...   222   e-104  2
(AU265923) Dictyostelium discoideum vegetative cDNA clone:VS...   222   e-104  2
dna update 2008.11.16
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(B0G125) RecName: Full=Uncharacterized protein DDB_G0295473;           62   2e-08
protein update 2009. 3.12
PSORT 

 psg: 0.93  gvh: 0.42  alm: 0.26  top: 0.60  tms: 0.07  mit: 0.52  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	  8.0 %: Golgi
	  8.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHJ144 is nuc
5' end seq. ID VHJ144F
5' end seq. 
>VHJ144F.Seq
ATATATATTCAATCTGAATCATTTTTTTTTATTTTTCATTTCAAAAAAATTACATTTCAA
TAATTCAAATTCATATTAAAATGTTTGGTTTTATATATAGAGACCCTTCTCCAGCACCTC
AAGGTAAAATTAGAGATGGTTCAAAAGACCCAAAAACACCAGGTGGTGGTGGTGGTGGTG
GTGGTGGTATTTCTCCAAATGGTGGTGCTCCCCTTGGTGGTAAAGGATTCTCAATGTAGT
TTTAACTTTATTTTGTTACAATTCGATAATTATTATTAAATCAAATAAAATTATTATATT
TGGTTCATCTTCTATTTTAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 338
3' end seq. ID VHJ144Z
3' end seq. 
>VHJ144Z.Seq
NNNNNNNNNNATATATATTCAATCTGAATCATTTTTTTTTATTTTTCATTTCAAAAAAAT
TACATTTCAATAATTCAAATTCATATTAAAATGTTTGGTTTTATATATAGAGACCCTTCT
CCAGCACCTCAAGGTAAAATTAGAGATGGTTCAAAAGACCCAAAAACACCAGGTGGTGGT
GGTGGTGGTGGTGGTGGTATTTCTCCAAATGGTGGTGCTCCCCTTGGTGGTAAAGGATTC
TCAATGTAGTTTTAACTTTATTTTGTTACAATTCGATAATTATTATTAATCAAATAAAAT
TATTATATTGGGTTCATC
Length of 3' end seq. 318
Connected seq. ID VHJ144P
Connected seq. 
>VHJ144P.Seq
ATATATATTCAATCTGAATCATTTTTTTTTATTTTTCATTTCAAAAAAATTACATTTCAA
TAATTCAAATTCATATTAAAATGTTTGGTTTTATATATAGAGACCCTTCTCCAGCACCTC
AAGGTAAAATTAGAGATGGTTCAAAAGACCCAAAAACACCAGGTGGTGGTGGTGGTGGTG
GTGGTGGTATTTCTCCAAATGGTGGTGCTCCCCTTGGTGGTAAAGGATTCTCAATGTAGT
TTTAACTTTATTTTGTTACAATTCGATAATTATTATTAAATCAAATAAAATTATTATATT
TGGTTCATCTTCTATTTTAAAAAAAAAA----------ATATATATTCAATCTGAATCAT
TTTTTTTTATTTTTCATTTCAAAAAAATTACATTTCAATAATTCAAATTCATATTAAAAT
GTTTGGTTTTATATATAGAGACCCTTCTCCAGCACCTCAAGGTAAAATTAGAGATGGTTC
AAAAGACCCAAAAACACCAGGTGGTGGTGGTGGTGGTGGTGGTGGTATTTCTCCAAATGG
TGGTGCTCCCCTTGGTGGTAAAGGATTCTCAATGTAGTTTTAACTTTATTTTGTTACAAT
TCGATAATTATTATTAATCAAATAAAATTATTATATTGGGTTCATC
Length of connected seq. 636
Full length Seq ID -
Full length Seq. -
Length of full length seq. -