>VHJ109 (VHJ109Q) /CSM/VH/VHJ1-A/VHJ109Q.Seq.d/
XXXXXXXXXXCTAATAATAATAATAATAATAATAGTCNAAATAATAATAATAATAATAAT
AATAATAATAATAATAATAATAGTAATAACCAATTTGAAAATATGATAAATTTAAAGAGT
CAAAATAGTTTTCAAAATATAAAAGAAGTTAAATTATTTAATAATAATATTGAAAGAGAG
ATGTATGAGAATTTAGCAGAGTTATATTCAATCATTAAAGTAACAGAACATTTAGAGAAA
GCATATATTCGTGATGATGTTTCACCAAAAGATTATACAACAGCATGTTCAAAATTAATT
GCACAATTTAAATCATCTCAAACACTCTTAAAGGATCAAGTTTCAAATGTTGGTCAATTC
ATGAAAGATTATGATTTAAATTGTAAAGCAGCTTTTGATAGATTAGTTATAAAAGGTTTT
CCATCAACATTAGAACATAATACAAATGAATCTTCAACTGATTCTGCAATGGCAAAAAAT
GTTGCTGAAGCAGTACAATTATTTATTACAACAATGGATTCAATTAGATTAAAACTTGTA
TCAGTTGATGGTATTTATCCACTTTTAAGTGATTTAATGGAAAGCTTAAATAAAAATCAA
T

---NNNNNNNSXNNNNNNNNNNNNNNSNNQFENMINLKSQNSFQNIKEVKLFNNNIEREM
YENLAELYSIIKVTEHLEKAYIRDDVSPKDYTTACSKLIAQFKSSQTLLKDQVSNVGQFM
KDYDLNCKAAFDRLVIKGFPSTLEHNTNESSTDSAMAKNVAEAVQLFITTMDSIRLKLVS
VDGIYPLLSDLMESLNKNQ

Frame A:
---********sk****************pi*kydkfkesk*fskykrs*ii***y*krd
v*efsrvifnh*snrtfresiys**cftkrlynsmfkincti*iisntlkgssfkcwsih
erl*fkl*ssf**isykrfsinirt*yk*ifn*fcngkkcc*sstiiyynngfn*iktci
s*wylstfk*fngklk*ks


Frame B:
---NNNNNNNSXNNNNNNNNNNNNNNSNNQFENMINLKSQNSFQNIKEVKLFNNNIEREM
YENLAELYSIIKVTEHLEKAYIRDDVSPKDYTTACSKLIAQFKSSQTLLKDQVSNVGQFM
KDYDLNCKAAFDRLVIKGFPSTLEHNTNESSTDSAMAKNVAEAVQLFITTMDSIRLKLVS
VDGIYPLLSDLMESLNKNQ


Frame C:
---iiiiiiivxiiiiiiiiiiiiiivitnlki**i*rvkivfki*kklnyliiilkerc
mri*qsyiqslk*qni*rkhifvmmfhqkiiqqhvqn*lhnlnhlkhs*rikfqmlvns*
kimi*ivkqllid*l*kvfhqh*niiqmnlqlilqwqkmllkqynyllqqwiqld*nlyq
lmvfihf*vi*wka*ikin

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHJ109 (VHJ109Q) /CSM/VH/VHJ1-A/VHJ109Q.Seq.d/                       1015   0.0
SSC151 (SSC151Q) /CSM/SS/SSC1-C/SSC151Q.Seq.d/                        985   0.0
AFL135 (AFL135Q) /CSM/AF/AFL1-B/AFL135Q.Seq.d/                        801   0.0
SSJ440 (SSJ440Q) /CSM/SS/SSJ4-B/SSJ440Q.Seq.d/                        581   e-165
SSM315 (SSM315Q) /CSM/SS/SSM3-A/SSM315Q.Seq.d/                         40   0.038
SSH341 (SSH341Q) /CSM/SS/SSH3-B/SSH341Q.Seq.d/                         40   0.038
FC-IC0814 (FC-IC0814Q) /CSM/FC-IC/FC-IC0814Q.Seq.d/                    40   0.038
VHA572 (VHA572Q) /CSM/VH/VHA5-C/VHA572Q.Seq.d/                         38   0.15
SFK809 (SFK809Q) /CSM/SF/SFK8-A/SFK809Q.Seq.d/                         38   0.15
VSK328 (VSK328Q) /CSM/VS/VSK3-B/VSK328Q.Seq.d/                         36   0.59

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.    34   6e-07  16
AP003193|AP003193.2 Clostridium perfringens str. 13 DNA, complete genome, section 9/10.    34   6e-05  12
AE014825|AE014825.1 Plasmodium falciparum 3D7 chromosome 14 section 10 of 13 of the complete sequence.    36   1e-04  12
AC116977|AC116977.2 Dictyostelium discoideum chromosome 2 map 5515173-5817331 strain AX4, complete sequence.    38   2e-04  14
AE014842|AE014842.1 Plasmodium falciparum 3D7 chromosome 11 section 7 of 8 of the complete sequence.    32   2e-04  13
AC012392|AC012392.1 Genomic Sequence For Arabidopsis thaliana clone C17L7, Chromosome IV, complete sequence.    52   2e-04  10
AB063521|AB063521.2 Wigglesworthia brevipalpis DNA, complete genome, section 1/2.    32   6e-04  14
AL844509|AL844509.1 Plasmodium falciparum chromosome 13.           34   0.001  20
AP004170|AP004170.1 Mycoplasma penetrans genomic DNA, complete sequence, section 1/5.    30   0.001  14
BX000443|BX000443.5 Zebrafish DNA sequence from clone DKEY-5K18 in linkage group 17.    38   0.002  7

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54NF1) RecName: Full=Vacuolar protein sorting-associated prote...   333   2e-90
AF162444_5(AF162444|pid:none) Arabidopsis thaliana BAC T32N4.  &...   163   4e-39
AM456840_1(AM456840|pid:none) Vitis vinifera contig VV78X128867....   159   7e-38
EF145612_1(EF145612|pid:none) Populus trichocarpa clone WS0112_A...   158   1e-37
AK342379_1(AK342379|pid:none) Acyrthosiphon pisum ACYPI009739 mR...   125   7e-28
EU969156_1(EU969156|pid:none) Zea mays clone 326458 VPS28-like p...   122   6e-27
AY813327_1(AY813327|pid:none) Schistosoma japonicum SJCHGC01553 ...   118   1e-25
AY813217_1(AY813217|pid:none) Schistosoma japonicum clone SJCHGC...   118   1e-25
AE017341_644(AE017341|pid:none) Cryptococcus neoformans var. neo...   117   3e-25
BT077377_1(BT077377|pid:none) Lepeophtheirus salmonis Pacific fo...   116   4e-25

 psg: 0.51  gvh: 0.21  alm: 0.44  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 44.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  8.0 %: cytoskeletal

>> prediction for VHJ109 is cyt

>VHJ109Z.Seq
NNNNNNNNNNCTAATAATAATAATAATAATAATAGTCNAAATAATAATAATAATAATAAT
AATAATAATAATAATAATAATAGTAATAACCAATTTGAAAATATGATAAATTTAAAGAGT
CAAAATAGTTTTCAAAATATAAAAGAAGTTAAATTATTTAATAATAATATTGAAAGAGAG
ATGTATGAGAATTTAGCAGAGTTATATTCAATCATTAAAGTAACAGAACATTTAGAGAAA
GCATATATTCGTGATGATGTTTCACCAAAAGATTATACAACAGCATGTTCAAAATTAATT
GCACAATTTAAATCATCTCAAACACTCTTAAAGGATCAAGTTTCAAATGTTGGTCAATTC
ATGAAAGATTATGATTTAAATTGTAAAGCAGCTTTTGATAGATTAGTTATAAAAGGTTTT
CCATCAACATTAGAACATAATACAAATGAATCTTCAACTGATTCTGCAATGGCAAAAAAT
GTTGCTGAAGCAGTACAATTATTTATTACAACAATGGATTCAATTAGATTAAAACTTGTA
TCAGTTGATGGTATTTATCCACTTTTAAGTGATTTAATGGAAAGCTTAAATAAAAATCAA
T