NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI879
Library VH
(Link to library)
Clone ID VHI879
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHI879P
(Link to Original site)
Representative DNA sequence 
>VHI879 (VHI879Q) /CSM/VH/VHI8-D/VHI879Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
XXXXXXXXXXCTCGTCAGTGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTC
TTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAG
AGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGG
TGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCC
AACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGG
TATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGG
TTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGC
TGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGC
TTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAA
GAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAAC
AATTTAAGTCGCACTTTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAATTTTAAAA
AAAAAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGN---

---LVSVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGG
GQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIG
SPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRK
RKGLAPEIPDLDKFHEKL*ti*val*vfknsnqi*kkf*kkn
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllvt---

---LVSVTKGVQYLNEIKDSFVGAFQWATKEGVVCDENMRGIRFNLYDVTLHTDAIHRGG
GQIIPTARRVLYAAELTASPTXLEPXYLVEITAPENAIGGIYSVLNRRRGIVIGEERRIG
SPLFSVKAHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRK
RKGLAPEIPDLDKFHEKL*ti*val*vfknsnqi*kkf*kkn

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw*---

---ssvspkvfni*mklkilslvlsngppkkvlfvmkt*evsvsifmmspstlmpstevv
vkssqllvvysmlln*lppqlx*nqxt**kllpqktllvvftlysivvevlllvknvvsv
physlskliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkplksl*pper
ekv*pqksqiltnstknskqfkshfefskivikskknfkkki

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVGN---

---rqchqrcsife*n*rffrwcfpmghqrrccl**kherypfqsl*chpph*chpqrww
snhpncssctlcc*inclpnxxrtnxlsrnycprkrywwyllctqss*rycyw*rtsyrf
piilcqssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*srfshqke
krfsprnprs*qiprktlnnlsrtlsfqk**snlkkilkkk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI879 (VHI879Q) /CSM/VH/VHI8-D/VHI879Q.Seq.d/                       2349   0.0
VHP832 (VHP832Q) /CSM/VH/VHP8-B/VHP832Q.Seq.d/                       1231   0.0
VHN502 (VHN502Q) /CSM/VH/VHN5-A/VHN502Q.Seq.d/                       1231   0.0
VHJ652 (VHJ652Q) /CSM/VH/VHJ6-C/VHJ652Q.Seq.d/                       1231   0.0
VHH827 (VHH827Q) /CSM/VH/VHH8-B/VHH827Q.Seq.d/                       1231   0.0
VHE120 (VHE120Q) /CSM/VH/VHE1-A/VHE120Q.Seq.d/                       1231   0.0
VHD321 (VHD321Q) /CSM/VH/VHD3-A/VHD321Q.Seq.d/                       1231   0.0
VHD214 (VHD214Q) /CSM/VH/VHD2-A/VHD214Q.Seq.d/                       1231   0.0
VHB829 (VHB829Q) /CSM/VH/VHB8-B/VHB829Q.Seq.d/                       1231   0.0
VHH874 (VHH874Q) /CSM/VH/VHH8-D/VHH874Q.Seq.d/                       1225   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442130) Dictyostelium discoideum cDNA clone:ddv48n20, 3' ...  1239   0.0    1
(BJ446358) Dictyostelium discoideum cDNA clone:ddv62p08, 3' ...  1231   0.0    1
(BJ444961) Dictyostelium discoideum cDNA clone:ddv58c01, 3' ...  1231   0.0    1
(BJ442655) Dictyostelium discoideum cDNA clone:ddv50g14, 3' ...  1231   0.0    1
(BJ441306) Dictyostelium discoideum cDNA clone:ddv46f08, 3' ...  1231   0.0    1
(BJ438985) Dictyostelium discoideum cDNA clone:ddv39g05, 3' ...  1231   0.0    1
(BJ438509) Dictyostelium discoideum cDNA clone:ddv37k04, 3' ...  1231   0.0    1
(BJ438505) Dictyostelium discoideum cDNA clone:ddv37j05, 3' ...  1231   0.0    1
(BJ437469) Dictyostelium discoideum cDNA clone:ddv34j08, 3' ...  1231   0.0    1
(BJ436477) Dictyostelium discoideum cDNA clone:ddv31e10, 3' ...  1231   0.0    1
dna update 2008.11.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   263   8e-69
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   258   2e-67
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   258   2e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   9e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   256   1e-66
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   2e-66
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   2e-66
AY391422_1(AY391422|pid:none) Danio rerio eukaryotic translation...   254   6e-66
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   253   8e-66
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   253   8e-66
protein update 2009. 7.14
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 36.0 %: nuclear
	  4.0 %: extracellular, including cell wall
	  4.0 %: cytoskeletal
	  4.0 %: vacuolar

>> prediction for VHI879 is cyt
5' end seq. ID VHI879F
5' end seq. 
>VHI879F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
NNNNNNNNNN
Length of 5' end seq. 610
3' end seq. ID VHI879Z
3' end seq. 
>VHI879Z.Seq
NNNNNNNNNNCTCGTCAGTGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTC
TTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAG
AGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGG
TGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCC
AACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGG
TATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGG
TTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGC
TGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGC
TTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAA
GAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAAC
AATTTAAGTCGCACTTTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAATTTTAAAA
AAAAAATA
Length of 3' end seq. 668
Connected seq. ID VHI879P
Connected seq. 
>VHI879P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAACA
----------CTCGTCAGTGTCACCAAAGGTGTTCAATATTTGAATGAAATTAAAGATTC
TTTCGTTGGTGCTTTCCAATGGGCCACCAAAGAAGGTGTTGTTTGTGATGAAAACATGAG
AGGTATCCGTTTCAATCTTTATGATGTCACCCTCCACACTGATGCCATCCACAGAGGTGG
TGGTCAAATCATCCCAACTGCTCGTCGTGTACTCTATGCTGCTGAATTAACTGCCTCCCC
AACTNTNTTAGAACCAATNTACTTAGTAGAAATTACTGCCCCAGAAAACGCTATTGGTGG
TATTTACTCTGTACTCAATCGTCGTAGAGGTATTGTTATTGGTGAAGAACGTCGTATCGG
TTCCCCATTATTCTCTGTCAAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGC
TGATTTACGTTCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGC
TTCAATTGGTGTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAA
GAGAAAAGGTTTAGCCCCAGAAATCCCAGATCTTGACAAATTCCACGAAAAACTCTAAAC
AATTTAAGTCGCACTTTGAGTTTTCAAAAATAGTAATCAAATCTAAAAAAAATTTTAAAA
AAAAAATA
Length of connected seq. 1258
Full length Seq ID -
Full length Seq. -
Length of full length seq. -