>VHI863 (VHI863Q) /CSM/VH/VHI8-C/VHI863Q.Seq.d/
XXXXXXXXXXCTCTTCGTTTCATTCGTAACTTTAAACAACAAGTCGATAGTGGTGTCATC
AGTTTGGATGAAAATAAATGGATTGCAATTGGTTCAAAGATTTTCACCTTTGTCAGTGAA
ACCATCAAAGCTTTGGCCGATATCAAATTGGCCGATCTTAGCTTTAGATTATACCTTCAA
GCACTCCAAACCTTTGACCACTGTGGTTTGGTCCCTCGTGTCAAAGAATTGGCCATCAAG
GCTCTCTTAATCTTCCAAGAAGATATCGCCGATTTCAAAGCTCAAGTTATGGCCCTTCAA
TTGTTAATCTCTACTCTCAATAGTTTAAGCATTCCAAATGAAGAGATCTATGAATCCTTG
GCCGCCCAAACCATTAAACAAGCTTCTCGTCTTCTCTTACCACAAGATCAAGCAAAACTC
ATCTCTACTTGTTCACATTTATTCTGGGTTGATAATCCTTCTCGTCAATATCAAAATCCA
GACTCTGTATTACAAGCTCTCAAAAAAGCTCTCTCCATTATCAGTAATGAATCGAGTCCT
GGTCTTGGTACATTTGTTGACATTTTGAATGAATGTCTCTTCTATTGTGATAAAGAAACT
GATGCTGTTCCAATTCAATTCGTTTCAGATTTGGTTGAATTAATTCGTACTACTCATGTT
AAAGAAGCTGATCCAGCATTACCATATTTACAAAATACTATCAAATACATTCAATCTCAA
AATTATAAAGGTATTTCAATTTAAA

---LRFIRNFKQQVDSGVISLDENKWIAIGSKIFTFVSETIKALADIKLADLSFRLYLQA
LQTFDHCGLVPRVKELAIKALLIFQEDIADFKAQVMALQLLISTLNSLSIPNEEIYESLA
AQTIKQASRLLLPQDQAKLISTCSHLFWVDNPSRQYQNPDSVLQALKKALSIISNESSPG
LGTFVDILNECLFYCDKETDAVPIQFVSDLVELIRTTHVKEADPALPYLQNTIKYIQSQN
YKGISI*

Frame A:
---ssfhs*l*ttsr*wchqfg*k*mdcnwfkdfhlcq*nhqsfgryqigrs*l*iipss
tpnl*plwfgpscqrighqgslnlprryrrfqsssygpsivnlysq*fkhsk*rdl*ilg
rpnh*tsfssslttrsskthlylftfilg**sfssisksrlcitssqksslhyq**iesw
swyic*hfe*mslll**rn*ccsnsirfrfg*insyysc*rs*ssitiftkyyqihsisk
l*ryfnl


Frame B:
---LRFIRNFKQQVDSGVISLDENKWIAIGSKIFTFVSETIKALADIKLADLSFRLYLQA
LQTFDHCGLVPRVKELAIKALLIFQEDIADFKAQVMALQLLISTLNSLSIPNEEIYESLA
AQTIKQASRLLLPQDQAKLISTCSHLFWVDNPSRQYQNPDSVLQALKKALSIISNESSPG
LGTFVDILNECLFYCDKETDAVPIQFVSDLVELIRTTHVKEADPALPYLQNTIKYIQSQN
YKGISI*


Frame C:
---fvsfvtlnnksivvssvwmkinglqlvqrfsplsvkpsklwpisnwpilaldytfkh
skplttvvwslvsknwpsrls*sskkispisklklwpfnc*sllsiv*afqmkrsmnpwp
pkplnkllvfsyhkikqnssllvhiysgliillvnikiqtlyyklskklsplsvmnrvlv
lvhlltf*mnvssivikklmlfqfnsfqiwln*fvllmlkkliqhyhiykilsntfnlki
ikvfqfk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI863 (VHI863Q) /CSM/VH/VHI8-C/VHI863Q.Seq.d/                       1457   0.0
VHK662 (VHK662Q) /CSM/VH/VHK6-C/VHK662Q.Seq.d/                       1449   0.0
VHI583 (VHI583Q) /CSM/VH/VHI5-D/VHI583Q.Seq.d/                       1449   0.0
AHA519 (AHA519Q) /CSM/AH/AHA5-A/AHA519Q.Seq.d/                       1449   0.0
VHH532 (VHH532Q) /CSM/VH/VHH5-B/VHH532Q.Seq.d/                       1433   0.0
CFF491 (CFF491Q) /CSM/CF/CFF4-D/CFF491Q.Seq.d/                       1433   0.0
AHH565 (AHH565Q) /CSM/AH/AHH5-C/AHH565Q.Seq.d/                       1431   0.0
AHO859 (AHO859Q) /CSM/AH/AHO8-C/AHO859Q.Seq.d/                       1423   0.0
AHJ343 (AHJ343Q) /CSM/AH/AHJ3-B/AHJ343Q.Seq.d/                       1417   0.0
VHJ813 (VHJ813Q) /CSM/VH/VHJ8-A/VHJ813Q.Seq.d/                       1390   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AL050350|AL050350.14 Human DNA sequence from clone RP1-261K5 on chromosome 6q21-22.1.    46   0.70   1
AQ304508|AQ304508.1 HS_2209_A2_F03_MR CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2209 Col=6 Row=K, genomic survey sequence.    46   0.70   1
AC115897|AC115897.4 Mus musculus clone RP24-444M16, WORKING DRAFT SEQUENCE, 9 ordered pieces.    36   1.7    5
BI512451|BI512451.1 BB160009A20B07.5 Bee Brain Normalized Library, BB16 Apis mellifera cDNA clone BB160009A20B07 5', mRNA sequence.    32   2.3    3
AC097504|AC097504.3 Homo sapiens BAC clone RP11-362F19 from 4, complete sequence.    44   2.8    1
BB534855|BB534855.2 Mus musculus 0 day neonate lung cDNA, RIKEN full-length enriched library, clone:E030040E10, 3' end partial sequence.    44   2.8    1
AC132330|AC132330.5 Mus musculus chromosome 14 clone RP24-213O22, complete sequence.    44   2.8    1
AC124767|AC124767.2 Mus musculus chromosome UNK clone RP23-206M12, WORKING DRAFT SEQUENCE, 3 unordered pieces.    44   2.8    1
AP003238|AP003238.4 Oryza sativa (japonica cultivar-group) genomic DNA, chromosome 1, PAC clone:P0401G10.    44   2.8    1
AC101215|AC101215.1 Mus musculus clone RP23-182K16, LOW-PASS SEQUENCE SAMPLING.    44   2.8    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54C24) RecName: Full=Vacuolar sorting protein 35;                   470   e-131
AC108906_23(AC108906|pid:none) Oryza sativa chromosome 3 BAC OSJ...   132   2e-29
AC149634_2(AC149634|pid:none) Medicago truncatula clone mth2-5i1...   132   2e-29
AM425621_1(AM425621|pid:none) Vitis vinifera contig VV78X235801....   130   3e-29
E84556(E84556)probable vacuolar sorting-associated protein [impo...   127   3e-28
BT008553_1(BT008553|pid:none) Arabidopsis thaliana clone U50265 ...   127   3e-28
BT061953_1(BT061953|pid:none) Zea mays full-length cDNA clone ZM...   126   8e-28
AB368490_1(AB368490|pid:none) Arabidopsis thaliana VPS35 mRNA fo...   123   7e-27
AK227620_1(AK227620|pid:none) Arabidopsis thaliana mRNA for vacu...   123   7e-27
(Q9EQH3) RecName: Full=Vacuolar protein sorting-associated prote...   122   9e-27

 psg: 0.68  gvh: 0.48  alm: 0.38  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 24.0 %: mitochondrial
	 24.0 %: cytoplasmic
	  4.0 %: cytoskeletal

>> prediction for VHI863 is nuc

>VHI863Z.Seq
NNNNNNNNNNCTCTTCGTTTCATTCGTAACTTTAAACAACAAGTCGATAGTGGTGTCATC
AGTTTGGATGAAAATAAATGGATTGCAATTGGTTCAAAGATTTTCACCTTTGTCAGTGAA
ACCATCAAAGCTTTGGCCGATATCAAATTGGCCGATCTTAGCTTTAGATTATACCTTCAA
GCACTCCAAACCTTTGACCACTGTGGTTTGGTCCCTCGTGTCAAAGAATTGGCCATCAAG
GCTCTCTTAATCTTCCAAGAAGATATCGCCGATTTCAAAGCTCAAGTTATGGCCCTTCAA
TTGTTAATCTCTACTCTCAATAGTTTAAGCATTCCAAATGAAGAGATCTATGAATCCTTG
GCCGCCCAAACCATTAAACAAGCTTCTCGTCTTCTCTTACCACAAGATCAAGCAAAACTC
ATCTCTACTTGTTCACATTTATTCTGGGTTGATAATCCTTCTCGTCAATATCAAAATCCA
GACTCTGTATTACAAGCTCTCAAAAAAGCTCTCTCCATTATCAGTAATGAATCGAGTCCT
GGTCTTGGTACATTTGTTGACATTTTGAATGAATGTCTCTTCTATTGTGATAAAGAAACT
GATGCTGTTCCAATTCAATTCGTTTCAGATTTGGTTGAATTAATTCGTACTACTCATGTT
AAAGAAGCTGATCCAGCATTACCATATTTACAAAATACTATCAAATACATTCAATCTCAA
AATTATAAAGGTATTTCAATTTAAA