>VHI858 (VHI858Q) /CSM/VH/VHI8-C/VHI858Q.Seq.d/
ATTATTTTATTTTATAAGCTTTGAAGAAAATGAGAGAATTTTTAAAAGATGATTATGATG
TAATAGTTTGTGGTGGTGGTCCAGTTGGTTTGGCTACTGCATACAGATGTGCCAAAGCTG
GAAAGAAAGTACTTTGTCTCGAAAAATCAGTATTCTTTAATGGAGGTGGTAGTAGTGGTG
ATGTTGTTAGAATGTTACGTACAATGTACACTGAAGATTATATGGCCGATTTAGCACATG
AAACCCTTGGACTCTGGAAAGAACTTGGTGATGATGCTGGTGAAGGTGATTTAGTTTGGA
TGACAGGTCTCCTAAATTTTGGTGATCCAAATTATGGTGCAGGTGGTCCAGAAGGTACTT
TATTAGGACCAATTCCAAATTTAGAGAGATTGGGTATGCAATACAAAGTGTTAACTGCTC
AAGAAATTATGGAAGAGTATCCATTTAGAAACATTCCATCCAATCACCAAGGTGTTTTTG
CTCCAGACAATGGTGTTATCAATTTACCATTGGTATTACGTTCTCTTTACAAGTTATGTT
TACAATATGGTTGTAAAATGGTAAGCCATGXXXXXXXXXX

yfil*ALKKMREFLKDDYDVIVCGGGPVGLATAYRCAKAGKKVLCLEKSVFFNGGGSSGD
VVRMLRTMYTEDYMADLAHETLGLWKELGDDAGEGDLVWMTGLLNFGDPNYGAGGPEGTL
LGPIPNLERLGMQYKVLTAQEIMEEYPFRNIPSNHQGVFAPDNGVINLPLVLRSLYKLCL
QYGCKMVSH---

Frame A:
iilfykl*rk*enf*kmimm**fvvvvqlvwllhtdvpklerkyfvsknqyslmevvvvv
mllecyvqctlkiiwpi*hmkpldsgknlvmmlvkvi*fg*qvs*ilviqimvqvvqkvl
y*dqfqi*rdwvcntkc*llkklwksihletfhpitkvfllqtmvlsiyhwyyvlftsyv
ynmvvkw*a---


Frame B:
lfyfisfeenerifkr*l*cnslwwwsswfgyciqmcqswkestlsrkisil*wrw**w*
cc*nvtynvh*rlygrfst*npwtlertw**cw*r*fslddrspkfw*sklwcrwsrryf
irtnskfreigyaiqsvncsrnygrvsi*khsiqsprcfcsrqwcyqftigitfslqvmf
tiwl*ngkp---


Frame C:
yfil*ALKKMREFLKDDYDVIVCGGGPVGLATAYRCAKAGKKVLCLEKSVFFNGGGSSGD
VVRMLRTMYTEDYMADLAHETLGLWKELGDDAGEGDLVWMTGLLNFGDPNYGAGGPEGTL
LGPIPNLERLGMQYKVLTAQEIMEEYPFRNIPSNHQGVFAPDNGVINLPLVLRSLYKLCL
QYGCKMVSH---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ418 (VHQ418Q) /CSM/VH/VHQ4-A/VHQ418Q.Seq.d/                       1130   0.0
VHL890 (VHL890Q) /CSM/VH/VHL8-D/VHL890Q.Seq.d/                       1130   0.0
VHK414 (VHK414Q) /CSM/VH/VHK4-A/VHK414Q.Seq.d/                       1130   0.0
VHI858 (VHI858Q) /CSM/VH/VHI8-C/VHI858Q.Seq.d/                       1130   0.0
VHB454 (VHB454Q) /CSM/VH/VHB4-C/VHB454Q.Seq.d/                       1130   0.0
VHB252 (VHB252Q) /CSM/VH/VHB2-C/VHB252Q.Seq.d/                       1130   0.0
AHF386 (AHF386Q) /CSM/AH/AHF3-D/AHF386Q.Seq.d/                       1130   0.0
VHK762 (VHK762Q) /CSM/VH/VHK7-C/VHK762Q.Seq.d/                       1128   0.0
VHH234 (VHH234Q) /CSM/VH/VHH2-B/VHH234Q.Seq.d/                       1128   0.0
SHL131 (SHL131Q) /CSM/SH/SHL1-B/SHL131Q.Seq.d/                       1128   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ427626) Dictyostelium discoideum cDNA clone:ddv63d06, 5' ...  1128   0.0    1
(BJ425148) Dictyostelium discoideum cDNA clone:ddv54d24, 5' ...  1128   0.0    1
(BJ424503) Dictyostelium discoideum cDNA clone:ddv52l15, 5' ...  1128   0.0    1
(BJ424024) Dictyostelium discoideum cDNA clone:ddv51l04, 5' ...  1128   0.0    1
(BJ423238) Dictyostelium discoideum cDNA clone:ddv48d16, 5' ...  1128   0.0    1
(BJ418686) Dictyostelium discoideum cDNA clone:ddv33l14, 5' ...  1128   0.0    1
(BJ418663) Dictyostelium discoideum cDNA clone:ddv33g14, 5' ...  1128   0.0    1
(BJ397466) Dictyostelium discoideum cDNA clone:dds46m07, 5' ...  1128   0.0    1
(BJ333225) Dictyostelium discoideum cDNA clone:dda41l21, 5' ...  1128   0.0    1
(BJ422225) Dictyostelium discoideum cDNA clone:ddv45c10, 5' ...  1126   0.0    1

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

CU638744_413(CU638744|pid:none) Podospora anserina genomic DNA c...   206   5e-52
Y18574_4(Y18574|pid:none) Streptomyces tendae strain Tue901, nik...   109   5e-23
AM286415_1545(AM286415|pid:none) Yersinia enterocolitica subsp. ...    80   5e-14
CP001291_5186(CP001291|pid:none) Cyanothece sp. PCC 7424, comple...    78   2e-13
(Q29RU9) RecName: Full=Peroxisomal sarcosine oxidase;          S...    77   3e-13
CT978603_2294(CT978603|pid:none) Synechococcus sp. RCC307 genomi...    77   4e-13
AY892312_1(AY892312|pid:none) Synthetic construct Homo sapiens c...    76   6e-13
CR457155_1(CR457155|pid:none) Homo sapiens full open reading fra...    76   6e-13
AF134593_1(AF134593|pid:none) Homo sapiens L-pipecolic acid oxid...    76   6e-13
AX882278_1(AX882278|pid:none) Sequence 17183 from Patent EP10746...    76   6e-13

 psg: 0.67  gvh: 0.49  alm: 0.40  top: 0.53  tms: 0.00  mit: 0.26  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 40.0 %: nuclear
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHI858 is cyt

>VHI858F.Seq
ATTATTTTATTTTATAAGCTTTGAAGAAAATGAGAGAATTTTTAAAAGATGATTATGATG
TAATAGTTTGTGGTGGTGGTCCAGTTGGTTTGGCTACTGCATACAGATGTGCCAAAGCTG
GAAAGAAAGTACTTTGTCTCGAAAAATCAGTATTCTTTAATGGAGGTGGTAGTAGTGGTG
ATGTTGTTAGAATGTTACGTACAATGTACACTGAAGATTATATGGCCGATTTAGCACATG
AAACCCTTGGACTCTGGAAAGAACTTGGTGATGATGCTGGTGAAGGTGATTTAGTTTGGA
TGACAGGTCTCCTAAATTTTGGTGATCCAAATTATGGTGCAGGTGGTCCAGAAGGTACTT
TATTAGGACCAATTCCAAATTTAGAGAGATTGGGTATGCAATACAAAGTGTTAACTGCTC
AAGAAATTATGGAAGAGTATCCATTTAGAAACATTCCATCCAATCACCAAGGTGTTTTTG
CTCCAGACAATGGTGTTATCAATTTACCATTGGTATTACGTTCTCTTTACAAGTTATGTT
TACAATATGGTTGTAAAATGGTAAGCCATGNNNNNNNNNN