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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI856
Library VH
(Link to library)
Clone ID VHI856
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16306-1
Original site URL
Representative seq. ID VHI856P
(Link to Original site)
Representative DNA sequence 
>VHI856 (VHI856Q) /CSM/VH/VHI8-C/VHI856Q.Seq.d/
TGGCCTACTGGAAAAAAACAAATCAAATTTTATTTAAATAATATAAAAAAATAAATAATA
TAATATAAAAAATGATATCATCTCCAATTACCTCATGTACTGAGGCAATTGAAATGATCT
CAAAACTTCCACAAGAAGTATTTGAAAAAAGTACAAGAACAGCAACAAAGAAATTATTAG
CATCAATTGGTTTAGTGACAATTGGTACATATTTAGTATATGCACTTCCATGGTATTTGT
TACCAATTGGATGGATATTTATGGGTACAGCATGTTGTGGATTATTCTCTGTTGGGTATG
CATGTGGTAATGATTTATTTTTCAAGAATAAATTTGTTAACTATGTAGTTGGAACAATTT
GTATGTTACCATTAATGTATCCATTAGCCTATTGGAAGAAAAGAATTGAAAAGAAAGCAA
CAGCAAGTCGTGATTTCGTTCGTGAGATTGCAAGTGGTAATTTTTGGTTCCTCAGTTCAT
TACTTCAATGGTTATCATTAAATTTCAGTTTCAAATTCTCCAAATCAATGATGGTCTCAG
TCTCATTCCTTTATGTATTCATCGCATTATTCTTCCCAATTATGACCTACAGTATGGGCA
TTTGGGGTTTATTCAAGTACTATTTGATCCCATTGTTTGTGTXXXXXXXXXXATGGTATT
CATATGTCNGCTTACGATCCAGGTAAGTGGNTAATCAATTTCCTTAGTTGGTTTGGTTTC
GCCTATGAATTAAAGAGATTCCCAAGTGAATTATTCGCAAAAGGTANAATTCAAATGCAA
GAGANACCCATCAAAAAATTACGTGATCAACTCTATTGGGGTAAACCATTGGATCAATTA
CCATCATTCACTCGTGCTCAAGTTANAGACNNANGTCAAAAAGAAAAGAANCAACTCTTA
ATCAT
sequence update 2002.10.25
Translated Amino Acid sequence 
aywkktnqilfk*ykkinnii*KMISSPITSCTEAIEMISKLPQEVFEKSTRTATKKLLA
SIGLVTIGTYLVYALPWYLLPIGWIFMGTACCGLFSVGYACGNDLFFKNKFVNYVVGTIC
MLPLMYPLAYWKKRIEKKATASRDFVREIASGNFWFLSSLLQWLSLNFSFKFSKSMMVSV
SFLYVFIALFFPIMTYSMGIWGLFKYYLIPLFV---

---GIHMSAYDPGKWXINFLSWFGFAYELKRFPSELFAKGXIQMQEXPIKKLRDQLYWGK
PLDQLPSFTRAQVXDXXQKEKXQLLI
Translated Amino Acid sequence (All Frames) 
Frame A:
wptgkkqikfylnnikk*ii*ykk*yhlqlphvlrqlk*sqnfhkkylkkvqeqqqrny*
hqlv**qlvhi*ymhfhgicyqldgylwvqhvvdysllgmhvvmiyfsrinlltm*leqf
vcyh*cih*pigrkelkrkqqqvvisfvrlqvvifgssvhyfngyh*isvsnspnq*wsq
shsfmysshyssql*ptvwafgvyssti*shclc---

---mvficxltiqvsg*sislvglvspmn*rdsqvnysqkvxfkckrxpsknyvinsigv
nhwinyhhslvlklxtxvkkkrxns*s

Frame B:
glleknksnfi*ii*knk*ynikndiisnylmy*gn*ndlktstrsi*kkyknsnkeiis
inwfsdnwyifsictsmvfvtnwmdiygysmlwiilcwvcmw**fifqe*ic*lcswnnl
yvtinvsislleekn*kesnsks*frs*dckw*flvpqfitsmviikfqfqilqindgls
liplcihriilpnydlqyghlgfiqvlfdpivc---

---wysyvxlrsr*vxnqfp*lvwfrl*ikeipk*iirkrxnsnarxthqkit*stllg*
tigsitiihscssxrxxskrkextlnh

Frame C:
aywkktnqilfk*ykkinnii*KMISSPITSCTEAIEMISKLPQEVFEKSTRTATKKLLA
SIGLVTIGTYLVYALPWYLLPIGWIFMGTACCGLFSVGYACGNDLFFKNKFVNYVVGTIC
MLPLMYPLAYWKKRIEKKATASRDFVREIASGNFWFLSSLLQWLSLNFSFKFSKSMMVSV
SFLYVFIALFFPIMTYSMGIWGLFKYYLIPLFV---

---GIHMSAYDPGKWXINFLSWFGFAYELKRFPSELFAKGXIQMQEXPIKKLRDQLYWGK
PLDQLPSFTRAQVXDXXQKEKXQLLI
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI856 (VHI856Q) /CSM/VH/VHI8-C/VHI856Q.Seq.d/                       1538   0.0
VHP116 (VHP116Q) /CSM/VH/VHP1-A/VHP116Q.Seq.d/                       1130   0.0
VHM540 (VHM540Q) /CSM/VH/VHM5-B/VHM540Q.Seq.d/                       1130   0.0
VHD420 (VHD420Q) /CSM/VH/VHD4-A/VHD420Q.Seq.d/                       1130   0.0
VHB877 (VHB877Q) /CSM/VH/VHB8-D/VHB877Q.Seq.d/                       1130   0.0
SFI848 (SFI848Q) /CSM/SF/SFI8-B/SFI848Q.Seq.d/                       1130   0.0
VHK569 (VHK569Q) /CSM/VH/VHK5-C/VHK569Q.Seq.d/                       1124   0.0
VHH775 (VHH775Q) /CSM/VH/VHH7-D/VHH775Q.Seq.d/                       1122   0.0
VHD238 (VHD238Q) /CSM/VH/VHD2-B/VHD238Q.Seq.d/                       1122   0.0
AFG849 (AFG849Q) /CSM/AF/AFG8-C/AFG849Q.Seq.d/                       1122   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ427075) Dictyostelium discoideum cDNA clone:ddv61o03, 5' ...  1128   0.0    1
(BJ425665) Dictyostelium discoideum cDNA clone:ddv56o09, 5' ...  1128   0.0    1
(BJ423293) Dictyostelium discoideum cDNA clone:ddv48p14, 5' ...  1128   0.0    1
(BJ419754) Dictyostelium discoideum cDNA clone:ddv37h06, 5' ...  1128   0.0    1
(BJ419048) Dictyostelium discoideum cDNA clone:ddv34j20, 5' ...  1128   0.0    1
(BJ389365) Dictyostelium discoideum cDNA clone:dds18p12, 5' ...  1128   0.0    1
(BJ424493) Dictyostelium discoideum cDNA clone:ddv52i17, 5' ...  1122   0.0    1
(BJ422710) Dictyostelium discoideum cDNA clone:ddv46f19, 5' ...  1120   0.0    1
(BJ419838) Dictyostelium discoideum cDNA clone:ddv37k10, 5' ...  1120   0.0    1
(BJ325436) Dictyostelium discoideum cDNA clone:dda14b14, 5' ...  1120   0.0    1
dna update 2008.11.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

U09503_1(U09503|pid:none) Arabidopsis thaliana Columbia chloropl...    93   1e-17
AF229392_1(AF229392|pid:none) Arabidopsis thaliana cultivar Colu...    93   1e-17
AY647956_1(AY647956|pid:none) Arabidopsis lyrata subsp. petraea ...    93   1e-17
DQ904566_1(DQ904566|pid:none) Descurainia sophia omega-6 fatty a...    93   1e-17
AH2005(AH2005) phosphatidylcholine desaturase [imported] - Nosto...    92   3e-17
CP000117_4184(CP000117|pid:none) Anabaena variabilis ATCC 29413,...    92   3e-17
AF229389_1(AF229389|pid:none) Brassica oleracea cultivar Rapid C...    92   3e-17
AF229390_1(AF229390|pid:none) Brassica rapa cultivar R500 omega ...    91   4e-17
AF229391_1(AF229391|pid:none) Brassica napus cultivar Stellar om...    91   5e-17
AY642535_1(AY642535|pid:none) Brassica napus omega-6 desaturase ...    91   5e-17
protein update 2009. 7.14
PSORT 

 psg: 0.88  gvh: 0.50  alm: 0.24  top: 0.50  tms: 0.07  mit: 0.27  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.10  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 24.0 %: nuclear
	 24.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHI856 is nuc
5' end seq. ID VHI856F
5' end seq. 
>VHI856F.Seq
TGGCCTACTGGAAAAAAACAAATCAAATTTTATTTAAATAATATAAAAAAATAAATAATA
TAATATAAAAAATGATATCATCTCCAATTACCTCATGTACTGAGGCAATTGAAATGATCT
CAAAACTTCCACAAGAAGTATTTGAAAAAAGTACAAGAACAGCAACAAAGAAATTATTAG
CATCAATTGGTTTAGTGACAATTGGTACATATTTAGTATATGCACTTCCATGGTATTTGT
TACCAATTGGATGGATATTTATGGGTACAGCATGTTGTGGATTATTCTCTGTTGGGTATG
CATGTGGTAATGATTTATTTTTCAAGAATAAATTTGTTAACTATGTAGTTGGAACAATTT
GTATGTTACCATTAATGTATCCATTAGCCTATTGGAAGAAAAGAATTGAAAAGAAAGCAA
CAGCAAGTCGTGATTTCGTTCGTGAGATTGCAAGTGGTAATTTTTGGTTCCTCAGTTCAT
TACTTCAATGGTTATCATTAAATTTCAGTTTCAAATTCTCCAAATCAATGATGGTCTCAG
TCTCATTCCTTTATGTATTCATCGCATTATTCTTCCCAATTATGACCTACAGTATGGGCA
TTTGGGGTTTATTCAAGTACTATTTGATCCCATTGTTTGTGTNNNNNNNNNN
Length of 5' end seq. 652
3' end seq. ID VHI856Z
3' end seq. 
>VHI856Z.Seq
NNNNNNNNNNATGGTATTCATATGTCNGCTTACGATCCAGGTAAGTGGNTAATCAATTTC
CTTAGTTGGTTTGGTTTCGCCTATGAATTAAAGAGATTCCCAAGTGAATTATTCGCAAAA
GGTANAATTCAAATGCAAGAGANACCCATCAAAAAATTACGTGATCAACTCTATTGGGGT
AAACCATTGGATCAATTACCATCATTCACTCGTGCTCAAGTTANAGACNNANGTCAAAAA
GAAAAGAANCAACTCTTAATCAT
Length of 3' end seq. 263
Connected seq. ID VHI856P
Connected seq. 
>VHI856P.Seq
TGGCCTACTGGAAAAAAACAAATCAAATTTTATTTAAATAATATAAAAAAATAAATAATA
TAATATAAAAAATGATATCATCTCCAATTACCTCATGTACTGAGGCAATTGAAATGATCT
CAAAACTTCCACAAGAAGTATTTGAAAAAAGTACAAGAACAGCAACAAAGAAATTATTAG
CATCAATTGGTTTAGTGACAATTGGTACATATTTAGTATATGCACTTCCATGGTATTTGT
TACCAATTGGATGGATATTTATGGGTACAGCATGTTGTGGATTATTCTCTGTTGGGTATG
CATGTGGTAATGATTTATTTTTCAAGAATAAATTTGTTAACTATGTAGTTGGAACAATTT
GTATGTTACCATTAATGTATCCATTAGCCTATTGGAAGAAAAGAATTGAAAAGAAAGCAA
CAGCAAGTCGTGATTTCGTTCGTGAGATTGCAAGTGGTAATTTTTGGTTCCTCAGTTCAT
TACTTCAATGGTTATCATTAAATTTCAGTTTCAAATTCTCCAAATCAATGATGGTCTCAG
TCTCATTCCTTTATGTATTCATCGCATTATTCTTCCCAATTATGACCTACAGTATGGGCA
TTTGGGGTTTATTCAAGTACTATTTGATCCCATTGTTTGTGT----------ATGGTATT
CATATGTCNGCTTACGATCCAGGTAAGTGGNTAATCAATTTCCTTAGTTGGTTTGGTTTC
GCCTATGAATTAAAGAGATTCCCAAGTGAATTATTCGCAAAAGGTANAATTCAAATGCAA
GAGANACCCATCAAAAAATTACGTGATCAACTCTATTGGGGTAAACCATTGGATCAATTA
CCATCATTCACTCGTGCTCAAGTTANAGACNNANGTCAAAAAGAAAAGAANCAACTCTTA
ATCAT
Length of connected seq. 895
Full length Seq ID -
Full length Seq. -
Length of full length seq. -