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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI853
Library VH
(Link to library)
Clone ID VHI853
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHI853P
(Link to Original site)
Representative DNA sequence 
>VHI853 (VHI853Q) /CSM/VH/VHI8-C/VHI853Q.Seq.d/
ATATTAAAAAATGTCNTCANGTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGT
TTGGCAAAATGATCGTGTTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATC
TTATGTTGCATTTACTGATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGC
AATGAATCCAACCAATACAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGA
CNAAGAAGTTCAAAGTGATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGA
TAAGCCACATATTCAAGTTGAATTCCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAA
TCTCTTCAATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCA
TTAATAATGCTGTAATTACCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCA
AAGATGCTGGTACAATTTCAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTG
CTGCTATTGCTTATGGTTTAGAXXXXXXXXXXCTGATAATCAACCAGGTGTATTAATTCA
AGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACT
TTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGA
TGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGAT
TACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGA
TGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAA
ATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAA
AATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTT
AGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGC
AGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGC
sequence update 2002.10.25
Translated Amino Acid sequence 
ikkcxxvvli*vqhihvlvfgkmivlkllqmikvieplhlmlhlliqnv*lvmlqriklq
*iqpiqylmlnv*lvvnlvtkkfkvi*NIGHSKLFQRMVISHIFKLNSKGETKVFSPEEI
SSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNVQRIINEPTAA
AIAYGL---

---DNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVS
AEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTV
KNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSK
LYQEGGM
Translated Amino Acid sequence (All Frames) 
Frame A:
ilknvxxwy*frynifmcwclak*sc*nyck*sr**nhsilcciy*yrtfnw*cckessc
nesnqysi*c*tfnws*i**xrssk*yetlaiqsyskgw**atyss*ipkvklkfshqkk
slqwys*k*kkplklilvkpliml*lpfqlismivnvkqpkmlvqfqn*mfnvllmnqll
llllmv*---

---liinqvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhlmlmqmvs*my
qlkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnqrinwkimhsl
lkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp*kqlsiqs*v
nsikkevc

Frame B:
y*kmssxgidlgttyscvgvwqndrveiiandqgnrttpsyvaftdterligdaaknqva
mnptntvfdakrligrkfsdxevqsdmkhwpfkvipkdgdkphiqvefqr*n*sfltrrn
lfngtlknernr*slsw*nh**ccnyrsslfq**stssnqrcwynfkikcstyy**tncc
cyclwfr---

---**strcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi*c*ckwylkci
s*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*ike*igklcihc
*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*deslrsscqsnhe*
tlsrrry

Frame C:
ikkcxxvvli*vqhihvlvfgkmivlkllqmikvieplhlmlhlliqnv*lvmlqriklq
*iqpiqylmlnv*lvvnlvtkkfkvi*NIGHSKLFQRMVISHIFKLNSKGETKVFSPEEI
SSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNVQRIINEPTAA
AIAYGL---

---DNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTFDVDANGILNVS
AEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVESKNKLENYAFTV
KNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKALEAVVNPIMSK
LYQEGGM
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHQ260 (VHQ260Q) /CSM/VH/VHQ2-C/VHQ260Q.Seq.d/                       1100   0.0
VHQ240 (VHQ240Q) /CSM/VH/VHQ2-B/VHQ240Q.Seq.d/                       1100   0.0
VHP873 (VHP873Q) /CSM/VH/VHP8-D/VHP873Q.Seq.d/                       1100   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1100   0.0
VHP716 (VHP716Q) /CSM/VH/VHP7-A/VHP716Q.Seq.d/                       1100   0.0
VHP437 (VHP437Q) /CSM/VH/VHP4-B/VHP437Q.Seq.d/                       1100   0.0
VHP420 (VHP420Q) /CSM/VH/VHP4-A/VHP420Q.Seq.d/                       1100   0.0
VHP236 (VHP236Q) /CSM/VH/VHP2-B/VHP236Q.Seq.d/                       1100   0.0
VHP232 (VHP232Q) /CSM/VH/VHP2-B/VHP232Q.Seq.d/                       1100   0.0
VHP224 (VHP224Q) /CSM/VH/VHP2-A/VHP224Q.Seq.d/                       1100   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(X75263) D.discoideum (AX3) mRNA for heat shock cognate prot...  1100   0.0    1
(BJ446704) Dictyostelium discoideum cDNA clone:ddv63g16, 3' ...  1100   0.0    1
(BJ446664) Dictyostelium discoideum cDNA clone:ddv63o10, 3' ...  1100   0.0    1
(BJ446443) Dictyostelium discoideum cDNA clone:ddv62b20, 3' ...  1100   0.0    1
(BJ446275) Dictyostelium discoideum cDNA clone:ddv62p03, 3' ...  1100   0.0    1
(BJ446228) Dictyostelium discoideum cDNA clone:ddv62f05, 3' ...  1100   0.0    1
(BJ446038) Dictyostelium discoideum cDNA clone:ddv61o08, 3' ...  1100   0.0    1
(BJ446016) Dictyostelium discoideum cDNA clone:ddv61j10, 3' ...  1100   0.0    1
(BJ446000) Dictyostelium discoideum cDNA clone:ddv61g10, 3' ...  1100   0.0    1
(BJ445963) Dictyostelium discoideum cDNA clone:ddv61o06, 3' ...  1100   0.0    1
dna update 2008.11.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   332   2e-93
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   332   9e-93
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   253   5e-87
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   253   5e-87
AY495382_1(AY495382|pid:none) Trypanosoma rangeli isolate Tre he...   192   2e-79
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   219   3e-79
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   219   3e-79
(P11145) RecName: Full=Heat shock 70 kDa protein 4; AltName: Ful...   220   5e-79
EF442767_1(EF442767|pid:none) Trypanosoma rangeli isolate H14 cl...   227   9e-79
FJ168549_2(FJ168549|pid:none) Bodo saltans clone fosmid 16K02, c...   202   9e-79
protein update 2009. 7.14
PSORT 

 psg: 0.80  gvh: 0.33  alm: 0.48  top: 0.53  tms: 0.00  mit: 0.32  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 36.0 %: nuclear
	 16.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: peroxisomal

>> prediction for VHI853 is cyt
5' end seq. ID VHI853F
5' end seq. 
>VHI853F.Seq
ATATTAAAAAATGTCNTCANGTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGT
TTGGCAAAATGATCGTGTTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATC
TTATGTTGCATTTACTGATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGC
AATGAATCCAACCAATACAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGA
CNAAGAAGTTCAAAGTGATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGA
TAAGCCACATATTCAAGTTGAATTCCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAA
TCTCTTCAATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCA
TTAATAATGCTGTAATTACCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCA
AAGATGCTGGTACAATTTCAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTG
CTGCTATTGCTTATGGTTTAGANNNNNNNNNN
Length of 5' end seq. 572
3' end seq. ID VHI853Z
3' end seq. 
>VHI853Z.Seq
NNNNNNNNNNCTGATAATCAACCAGGTGTATTAATTCAAGTTTATGAAGGTGAACGTGCT
ATGACCAAAGATAATAATTTATTGGGTAAATTTGAACTTTCTGGTATTCCACCAGCACCA
AGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGATGCAAATGGTATCTTAAATGTA
TCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGATTACAATTACCAATGATAAAGGT
CGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGATGCAGAGAAATTCAAACAACAA
GATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAAATTGGAAAATTATGCATTCACT
GTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAAAATTTCAGATTCAGATAAATCA
ACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTTAGAATCAAATCAAACCGCAGAA
AAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGCAGTTGTCAATCCAATCATGAGT
AAACTCTATCAAGAAGGAGGTATGC
Length of 3' end seq. 565
Connected seq. ID VHI853P
Connected seq. 
>VHI853P.Seq
ATATTAAAAAATGTCNTCANGTGGTATTGATTTAGGTACAACATATTCATGTGTTGGTGT
TTGGCAAAATGATCGTGTTGAAATTATTGCAAATGATCAAGGTAATAGAACCACTCCATC
TTATGTTGCATTTACTGATACAGAACGTTTAATTGGTGATGCTGCAAAGAATCAAGTTGC
AATGAATCCAACCAATACAGTATTTGATGCTAAACGTTTAATTGGTCGTAAATTTAGTGA
CNAAGAAGTTCAAAGTGATATGAAACATTGGCCATTCAAAGTTATTCCAAAGGATGGTGA
TAAGCCACATATTCAAGTTGAATTCCAAAGGTGAAACTAAAGTTTTCTCACCAGAAGAAA
TCTCTTCAATGGTACTCTTAAAAATGAAAGAAACCGCTGAAGCTTATCTTGGTAAAACCA
TTAATAATGCTGTAATTACCGTTCCAGCTTATTTCAATGATAGTCAACGTCAAGCAACCA
AAGATGCTGGTACAATTTCAAAATTAAATGTTCAACGTATTATTAATGAACCAACTGCTG
CTGCTATTGCTTATGGTTTAGA----------CTGATAATCAACCAGGTGTATTAATTCA
AGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAACT
TTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTGA
TGCAAATGGTATCTTAAATGTATCAGCTGAAGATAAATCAACTGGTAACAAACAAAAGAT
TACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTGA
TGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATAA
ATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCAA
AATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGTT
AGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAGC
AGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGC
Length of connected seq. 1117
Full length Seq ID -
Full length Seq. -
Length of full length seq. -