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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI846
Library VH
(Link to library)
Clone ID VHI846
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16292-1|Contig-U16400-1
Original site URL
Representative seq. ID VHI846P
(Link to Original site)
Representative DNA sequence 
>VHI846 (VHI846Q) /CSM/VH/VHI8-B/VHI846Q.Seq.d/
AGGTGTGTTCACAAAAATAACAACAACACTAAAAAAAGTCTTCATTGTTTTTTACTTTTT
TTTTTAATTTTTAATTTTTTATTTAATTTAAACAATATATAAATCAATTAAAATAAACAC
TCACTCGATAGNGTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAANNNNTTTTNAAAAAAAAAXXXXXXXXXXAGGTCATGGGTGCCCATTGTGGTTAT
TTGGCTGCAATGAGTTCTTTAACCTCAGGTGCTGAACGTTCATATATAATGGAAAGAGGT
ATCACATTGAATACACTAACCAAGGATTTGGAAATGTTTGTTGAACGTTTCAAACGTGAA
CATAGAATTGGTCTAATTATTAAGAGTGAACTTGCTAGCAACACCTACTCAACTCATTTC
ATTTATTCACTCTTCAAAGAAGAAGGTAAACATTTATTCGATGTCCGTGAATCTATACTC
GGTCATTTACAACAAGGTGGTACTCCTTCTGCAATTGATCGTATCTTTTCAACTCGTTTA
ATGAATCATTATTATCAATTCTTGGAAAACGATCTCAAAGAACATGGTCATCTTCAAATG
AATGGTTGTATTGGTTTCATTGATGGTGGTATTCATTACACTCCAATGCAAGAAATGATC
GAAGAAATGTCTGATAAATTT
sequence update 2002.10.25
Translated Amino Acid sequence 
rcvhknnnntkkslhcfllfflifnflfnlnni*in*NKHSLDXVKEKKKKKKKKKKKKK
KKXXXKKK---

---VMGAHCGYLAAMSSLTSGAERSYIMERGITLNTLTKDLEMFVERFKREHRIGLIIKS
ELASNTYSTHFIYSLFKEEGKHLFDVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLE
NDLKEHGHLQMNGCIGFIDGGIHYTPMQEMIEEMSDKF
Translated Amino Acid sequence (All Frames) 
Frame A:
rcvhknnnntkkslhcfllfflifnflfnlnni*in*NKHSLDXVKEKKKKKKKKKKKKK
KKXXXKKK---

---rswvpivviwlq*vl*pqvlnvhi*wkevsh*ih*priwkcllnvsnvnielv*llr
vnllatptqlisfihsskkkvniysmsvnlysviynkvvlllqlivsfqlv**iiiinsw
ktisknmvifk*mvvlvslmvvfitlqckk*skkclin

Frame B:
gvftkitttlkkvfivfyfff*flifyli*tiyksikinthsix*kkkkkkkkkkkkkkk
kxxfxkk---

---ghgcplwlfgcneffnlrc*tfiyngkryhieytnqgfgnvc*tfqt*t*nwsny*e
*tc*qhllnsfhlftlqrrr*tfircp*iytrsfttrwysfcn*sylfnsfnesllsilg
krsqrtwsssnewlywfh*wwyslhsnarndrrnv**i

Frame C:
vcsqk*qqh*kksslfftfffnf*ffi*fkqyinqlk*tltr*xkrkkkkkkkkkkkkkk
kxxfxkk---

---VMGAHCGYLAAMSSLTSGAERSYIMERGITLNTLTKDLEMFVERFKREHRIGLIIKS
ELASNTYSTHFIYSLFKEEGKHLFDVRESILGHLQQGGTPSAIDRIFSTRLMNHYYQFLE
NDLKEHGHLQMNGCIGFIDGGIHYTPMQEMIEEMSDKF
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHD874 (VHD874Q) /CSM/VH/VHD8-D/VHD874Q.Seq.d/                        926   0.0
VHP708 (VHP708Q) /CSM/VH/VHP7-A/VHP708Q.Seq.d/                        926   0.0
VHP472 (VHP472Q) /CSM/VH/VHP4-C/VHP472Q.Seq.d/                        926   0.0
VHP463 (VHP463Q) /CSM/VH/VHP4-C/VHP463Q.Seq.d/                        926   0.0
VHO736 (VHO736Q) /CSM/VH/VHO7-B/VHO736Q.Seq.d/                        926   0.0
VHO650 (VHO650Q) /CSM/VH/VHO6-C/VHO650Q.Seq.d/                        926   0.0
VHN894 (VHN894Q) /CSM/VH/VHN8-D/VHN894Q.Seq.d/                        926   0.0
VHN560 (VHN560Q) /CSM/VH/VHN5-C/VHN560Q.Seq.d/                        926   0.0
VHM459 (VHM459Q) /CSM/VH/VHM4-C/VHM459Q.Seq.d/                        926   0.0
VHK413 (VHK413Q) /CSM/VH/VHK4-A/VHK413Q.Seq.d/                        926   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...   926   0.0    3
(X94340) D.discoideum mRNA for 6-phosphofructo-1-kinase.          926   0.0    1
(X89039) D.discoideum mRNA for 6-phosphofructo-1-kinase.          926   0.0    1
(BJ446273) Dictyostelium discoideum cDNA clone:ddv62p01, 3' ...   926   0.0    1
(BJ446121) Dictyostelium discoideum cDNA clone:ddv61p18, 3' ...   926   0.0    1
(BJ446110) Dictyostelium discoideum cDNA clone:ddv61n16, 3' ...   926   0.0    1
(BJ445746) Dictyostelium discoideum cDNA clone:ddv60c14, 3' ...   926   0.0    1
(BJ445699) Dictyostelium discoideum cDNA clone:ddv60h09, 3' ...   926   0.0    1
(BJ445248) Dictyostelium discoideum cDNA clone:ddv58l24, 3' ...   926   0.0    1
(BJ445146) Dictyostelium discoideum cDNA clone:ddv58g15, 3' ...   926   0.0    1
dna update 2008.11.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P90521) RecName: Full=6-phosphofructokinase;          Short=Pho...   317   2e-85
CP001337_2617(CP001337|pid:none) Chloroflexus aggregans DSM 9485...   132   1e-29
BC081652_1(BC081652|pid:none) Danio rerio phosphofructokinase, m...   118   1e-25
(Q867C9) RecName: Full=6-phosphofructokinase, muscle type;      ...   114   4e-24
DQ480147_1(DQ480147|pid:none) Sus scrofa muscle 6-phosphofructok...   112   8e-24
BC077756_1(BC077756|pid:none) Xenopus laevis MGC79063 protein, m...   112   8e-24
AY335614_1(AY335614|pid:none) Synthetic construct Homo sapiens p...   112   1e-23
CR858939_1(CR858939|pid:none) Pongo abelii mRNA; cDNA DKFZp468O1...   112   1e-23
(P08237) RecName: Full=6-phosphofructokinase, muscle type;      ...   112   1e-23
(Q0IIG5) RecName: Full=6-phosphofructokinase, muscle type;      ...   112   1e-23
protein update 2009. 7.14
PSORT 

 psg: 0.03  gvh: 0.62  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.25  mip: 0.00
 nuc: 0.74  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 80.0 %: nuclear
	 12.0 %: cytoskeletal
	  4.0 %: mitochondrial
	  4.0 %: cytoplasmic

>> prediction for VHI846 is nuc
5' end seq. ID VHI846F
5' end seq. 
>VHI846F.Seq
AGGTGTGTTCACAAAAATAACAACAACACTAAAAAAAGTCTTCATTGTTTTTTACTTTTT
TTTTTAATTTTTAATTTTTTATTTAATTTAAACAATATATAAATCAATTAAAATAAACAC
TCACTCGATAGNGTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAANNNNTTTTNAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 214
3' end seq. ID VHI846Z
3' end seq. 
>VHI846Z.Seq
NNNNNNNNNNAGGTCATGGGTGCCCATTGTGGTTATTTGGCTGCAATGAGTTCTTTAACC
TCAGGTGCTGAACGTTCATATATAATGGAAAGAGGTATCACATTGAATACACTAACCAAG
GATTTGGAAATGTTTGTTGAACGTTTCAAACGTGAACATAGAATTGGTCTAATTATTAAG
AGTGAACTTGCTAGCAACACCTACTCAACTCATTTCATTTATTCACTCTTCAAAGAAGAA
GGTAAACATTTATTCGATGTCCGTGAATCTATACTCGGTCATTTACAACAAGGTGGTACT
CCTTCTGCAATTGATCGTATCTTTTCAACTCGTTTAATGAATCATTATTATCAATTCTTG
GAAAACGATCTCAAAGAACATGGTCATCTTCAAATGAATGGTTGTATTGGTTTCATTGAT
GGTGGTATTCATTACACTCCAATGCAAGAAATGATCGAAGAAATGTCTGATAAATTT
Length of 3' end seq. 477
Connected seq. ID VHI846P
Connected seq. 
>VHI846P.Seq
AGGTGTGTTCACAAAAATAACAACAACACTAAAAAAAGTCTTCATTGTTTTTTACTTTTT
TTTTTAATTTTTAATTTTTTATTTAATTTAAACAATATATAAATCAATTAAAATAAACAC
TCACTCGATAGNGTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAANNNNTTTTNAAAAAAAAA----------AGGTCATGGGTGCCCATTGTGGTTAT
TTGGCTGCAATGAGTTCTTTAACCTCAGGTGCTGAACGTTCATATATAATGGAAAGAGGT
ATCACATTGAATACACTAACCAAGGATTTGGAAATGTTTGTTGAACGTTTCAAACGTGAA
CATAGAATTGGTCTAATTATTAAGAGTGAACTTGCTAGCAACACCTACTCAACTCATTTC
ATTTATTCACTCTTCAAAGAAGAAGGTAAACATTTATTCGATGTCCGTGAATCTATACTC
GGTCATTTACAACAAGGTGGTACTCCTTCTGCAATTGATCGTATCTTTTCAACTCGTTTA
ATGAATCATTATTATCAATTCTTGGAAAACGATCTCAAAGAACATGGTCATCTTCAAATG
AATGGTTGTATTGGTTTCATTGATGGTGGTATTCATTACACTCCAATGCAAGAAATGATC
GAAGAAATGTCTGATAAATTT
Length of connected seq. 671
Full length Seq ID -
Full length Seq. -
Length of full length seq. -