>VHI843 (VHI843Q) /CSM/VH/VHI8-B/VHI843Q.Seq.d/
XXXXXXXXXXTGGAAATGCAAAAGAGAATANCCAACTATCGTCCATCATGGAATTTANCC
GTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGAT
CCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTA
AGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGT
GTTGGCCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCAT
GATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCA
TTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAA
GGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACT
CTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAA
ATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTT
TCAACAATTACAATCGCTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCA
AATTTATCAAGATTAAAACGGAAAACTGTTGATGGTAAAAAC

---EMQKRIXNYRPSWNLXVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLR
DGQLDMMMHRRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPL
LPMFTTTQQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQI
DGQDSQDPQTITLDLSTIFSTITIASATEMNLTGVQKLSNLSRLKRKTVDGKN

Frame A:
---gnakenxqlssimefxrgstnqw*lctcecyclhsrs*siitihhch**kswmcivk
rwsigyddassyfer*wswcwptne*vnsnryn**idfp*yfklcsislsscctlfitsi
tpnvynhstifkrlefsisrclltidfsltftkwfkdsnstmvr*sr*yhtfty*kylsn
*wsrftrstnyhfrsfnylfnnynrfcn*nefnwcskiikfikiktenc*w*k


Frame B:
---EMQKRIXNYRPSWNLXVVQPTSGNYVPVNAIAYIQDPNQSLQFTIVTDRSRGCASLR
DGQLDMMMHRRTLKDDGRGVGQPMNESTQIVTTSKLIFHDISSYAQSHYRPAALSLSHPL
LPMFTTTQQSSNDWNSQYQGVYSPLTSASPLPNGLKIQTLQWLDNQDNTILLRIENIYQI
DGQDSQDPQTITLDLSTIFSTITIASATEMNLTGVQKLSNLSRLKRKTVDGKN


Frame C:
---kckrexptivhhgixpwfnqpvvimyl*mllptfkilinhynsplslievvdvhr*e
mvnwi**civvl*kmmvvvlanq*msqlkslqlvn*fsmifqvmlnliivllhslyhihy
sqclqplnnlqtigilnikvfthh*lqphlyqmv*rfklyng*iikiipyfyvlkifikl
mvkihkihklsl*ifqlsfqqlqsllqlk*i*lvfknyqiyqd*ngkllmvk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI843 (VHI843Q) /CSM/VH/VHI8-B/VHI843Q.Seq.d/                       1360   0.0
VHQ167 (VHQ167Q) /CSM/VH/VHQ1-C/VHQ167Q.Seq.d/                       1336   0.0
VHP443 (VHP443Q) /CSM/VH/VHP4-B/VHP443Q.Seq.d/                       1336   0.0
VHP436 (VHP436Q) /CSM/VH/VHP4-B/VHP436Q.Seq.d/                       1336   0.0
VHK690 (VHK690Q) /CSM/VH/VHK6-D/VHK690Q.Seq.d/                       1336   0.0
VHC754 (VHC754Q) /CSM/VH/VHC7-C/VHC754Q.Seq.d/                       1336   0.0
VHB462 (VHB462Q) /CSM/VH/VHB4-C/VHB462Q.Seq.d/                       1336   0.0
VHB425 (VHB425Q) /CSM/VH/VHB4-B/VHB425Q.Seq.d/                       1336   0.0
VHB391 (VHB391Q) /CSM/VH/VHB3-D/VHB391Q.Seq.d/                       1336   0.0
VHB333 (VHB333Q) /CSM/VH/VHB3-B/VHB333Q.Seq.d/                       1336   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

M82822|M82822.1 Dictyostelium discoideum alpha-mannosidase (manA) gene, complete cds.  1312   0.0    1
AC116984|AC116984.2 Dictyostelium discoideum chromosome 2 map 2567470-3108875 strain AX4, complete sequence.    36   0.003  14
AC115612|AC115612.2 Dictyostelium discoideum chromosome 2 map 6245135-6357017 strain AX4, complete sequence.    32   0.004  9
AC117076|AC117076.2 Dictyostelium discoideum chromosome 2 map 5862124-6045772 strain AX4, complete sequence.    38   0.083  9
BX572092|BX572092.1 Prochlorococcus marinus MED4 complete genome; segment 3/5.    32   0.13   12
AL161912|AL161912.15 Human DNA sequence from clone RP11-403N16 on chromosome 9 Contains a novel pseudogene.    34   0.32   4
AP000962|AP000962.2 Homo sapiens genomic DNA, chromosome 21q21.1-q21.2, clone:B821P16, LL56-APP region.    40   0.42   4
BS000028|BS000028.1 Pan troglodytes chromosome 22 clone:PTB-087F19, map 22, complete sequences.    40   0.66   5
AC094695|AC094695.10 Rattus norvegicus clone CH230-5F2, *** SEQUENCING IN PROGRESS ***, 3 unordered pieces.    36   0.68   5
AC114417|AC114417.4 Mus musculus clone RP23-356L3, WORKING DRAFT SEQUENCE, 9 ordered pieces.    36   1.4    5

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

M82822_1(M82822|pid:none) Dictyostelium discoideum alpha-mannosi...   398   e-110
(P34098) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   398   e-110
(Q29451) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   110   4e-23
AK068415_1(AK068415|pid:none) Oryza sativa Japonica Group cDNA c...   106   6e-22
BT036325_1(BT036325|pid:none) Zea mays full-length cDNA clone ZM...   106   6e-22
AY099704_1(AY099704|pid:none) Arabidopsis thaliana alpha-mannosi...   102   1e-20
AB005230_20(AB005230|pid:none) Arabidopsis thaliana genomic DNA,...   102   1e-20
AY036155_1(AY036155|pid:none) Cavia porcellus lysosomal alpha-ma...   102   1e-20
(Q8VHC8) RecName: Full=Lysosomal alpha-mannosidase;          Sho...   102   1e-20
AK291572_1(AK291572|pid:none) Homo sapiens cDNA FLJ76867 complet...   100   4e-20

 psg: 0.81  gvh: 0.50  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.35  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 32.0 %: cytoplasmic
	 32.0 %: mitochondrial
	 20.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: cytoskeletal
	  4.0 %: endoplasmic reticulum

>> prediction for VHI843 is cyt

>VHI843Z.Seq
NNNNNNNNNNTGGAAATGCAAAAGAGAATANCCAACTATCGTCCATCATGGAATTTANCC
GTGGTTCAACCAACCAGTGGTAATTATGTACCTGTGAATGCTATTGCCTACATTCAAGAT
CCTAATCAATCATTACAATTCACCATTGTCACTGATAGAAGTCGTGGATGTGCATCGTTA
AGAGATGGTCAATTGGATATGATGATGCATCGTCGTACTTTGAAAGATGATGGTCGTGGT
GTTGGCCAACCAATGAATGAGTCAACTCAAATCGTTACAACTAGTAAATTGATTTTCCAT
GATATTTCAAGTTATGCTCAATCTCATTATCGTCCTGCTGCACTCTCTTTATCACATCCA
TTACTCCCAATGTTTACAACCACTCAACAATCTTCAAACGATTGGAATTCTCAATATCAA
GGTGTTTACTCACCATTGACTTCAGCCTCACCTTTACCAAATGGTTTAAAGATTCAAACT
CTACAATGGTTAGATAATCAAGATAATACCATACTTTTACGTATTGAAAATATTTATCAA
ATTGATGGTCAAGATTCACAAGATCCACAAACTATCACTTTAGATCTTTCAACTATCTTT
TCAACAATTACAATCGCTTCTGCAACTGAAATGAATTTAACTGGTGTTCAAAAATTATCA
AATTTATCAAGATTAAAACGGAAAACTGTTGATGGTAAAAAC