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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO280
Library AF
(Link to library)
Clone ID AFO280
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13986-1
Original site URL
Representative seq. ID AFO280E
(Link to Original site)
Representative DNA sequence 
>AFO280 (AFO280Q) /CSM/AF/AFO2-D/AFO280Q.Seq.d/
AATTTAATACAGCATATAATTCATTCATACATTTAATCAAACAATGAATTCTACTCGTAA
TATCATTTCATTAGTTCGTCGTTATTCAACTTCAAGAAGTGTTAAAATCGCTTCACATGG
TTCACCATCAACTGCTTTAAAGATTGAAAATGAAAATATTACAGATAAAATTTCAAATAA
AGATGTTTTAGTTGAAATGTTACATGCACCAATTAATCCAGCAGATTTAAATATTATTCA
AGGTACTTATGGTACAAATGTTCAAGTTGGAGGTGTAGCAGGTATGGAAGGTGTTGGTGT
TGTTAAAAAGGTTGGTAGTGGTGTTACAGGATTAAAAGAGAATGATTTAGTAGTACCATC
AATGAAACAACACTTTGGATCATGGAGAAGTAAAGGTGTTTGGAGTGAACAACAATTATT
TAAAGTACCATCAGACATTCCAACCGAATACTTATCCACCATTTCAATCAATCCAACCAC
TGCCTATTTGTTATTGAACGATTTCGTAAAGTTACAACAAGGCGATGTCATCATTCNNAA
CNGCATCCAACTCAATGGTTGGTTTGTCAGTCATTCAATTGGCAAAGGCACGTGGTATCA
AAACCATCAATGTCATTCAGTGACGGTTCAGAATTCGAAGACAACGTCCAACGTCTCAAG
CAGTTGGGTGG
sequence update 2001. 6. 9
Translated Amino Acid sequence 
i*ysi*FIHTFNQTMNSTRNIISLVRRYSTSRSVKIASHGSPSTALKIENENITDKISNK
DVLVEMLHAPINPADLNIIQGTYGTNVQVGGVAGMEGVGVVKKVGSGVTGLKENDLVVPS
MKQHFGSWRSKGVWSEQQLFKVPSDIPTEYLSTISINPTTAYLLLNDFVKLQQGDVIIXN
XIQLNGWFVSHSIGKGTWYQNHQCHSVTVQNSKTTSNVSSSWV
Translated Amino Acid sequence (All Frames) 
Frame A:
nliqhiihsyi*snnefys*yhfissslfnfkkc*nrftwftincfkd*k*kyyr*nfk*
rcfs*nvtctn*ssrfkyysrylwykcsswrcsrygrcwcc*kgw*wcyrikre*fssti
nettlwimek*rcle*ttii*stirhsnrilihhfnqsnhclfvierfrkvttrrchhsx
xhptqwlvcqsfnwqrhvvskpsmsfsdgsefednvqrlkqlg


Frame B:
i*ysi*FIHTFNQTMNSTRNIISLVRRYSTSRSVKIASHGSPSTALKIENENITDKISNK
DVLVEMLHAPINPADLNIIQGTYGTNVQVGGVAGMEGVGVVKKVGSGVTGLKENDLVVPS
MKQHFGSWRSKGVWSEQQLFKVPSDIPTEYLSTISINPTTAYLLLNDFVKLQQGDVIIXN
XIQLNGWFVSHSIGKGTWYQNHQCHSVTVQNSKTTSNVSSSWV


Frame C:
fntaynsfihlikq*illvisfh*fvviqlqevlkslhmvhhqll*rlkmkilqikfqik
mf*lkcymhqliqqi*ilfkvlmvqmfklev*qvwkvlvllkrlvvvlqd*krmi**yhq
*nntldhgevkvfgvnnnylkyhqtfqpntyppfqsiqplpicy*tis*synkamssfxt
asnsmvglsviqlakargiktinviq*rfrirrqrptsqavgw
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO280 (AFO280Q) /CSM/AF/AFO2-D/AFO280Q.Seq.d/                       1312   0.0
VFN664 (VFN664Q) /CSM/VF/VFN6-C/VFN664Q.Seq.d/                       1291   0.0
VFK669 (VFK669Q) /CSM/VF/VFK6-C/VFK669Q.Seq.d/                       1287   0.0
VFI824 (VFI824Q) /CSM/VF/VFI8-A/VFI824Q.Seq.d/                       1287   0.0
VFF748 (VFF748Q) /CSM/VF/VFF7-B/VFF748Q.Seq.d/                       1287   0.0
VFF332 (VFF332Q) /CSM/VF/VFF3-B/VFF332Q.Seq.d/                       1287   0.0
VHK580 (VHK580Q) /CSM/VH/VHK5-D/VHK580Q.Seq.d/                       1277   0.0
VFO582 (VFO582Q) /CSM/VF/VFO5-D/VFO582Q.Seq.d/                       1277   0.0
VFL162 (VFL162Q) /CSM/VF/VFL1-C/VFL162Q.Seq.d/                       1271   0.0
VFA309 (VFA309Q) /CSM/VF/VFA3-A/VFA309Q.Seq.d/                       1267   0.0
own update 2003. 5.17
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

BX470264|BX470264.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-252F13.    38  0.37  4
AL445473|AL445473.7 Human DNA sequence from clone RP11-214M7 on chromosome 1.    36  0.50  4
BF059813|T26323 hypothetical protein W09H1.5 - Caenorhabditis elegans, mRNA sequence.    46  0.56  1
CA958218|CA958218.1 px57a01.y1 Haemonchus contortus intestine SL2 TOPO v1 Haemonchus contortus cDNA 5' similar to TR:O45903 O45903 W09H1.5 PROTEIN. [1] ;, mRNA sequence.    46  0.56  1
CA957710|CA957710.1 px56a01.y1 Haemonchus contortus intestine SL2 TOPO v1 Haemonchus contortus cDNA 5' similar to TR:O45903 O45903 W09H1.5 PROTEIN. [1] ;, mRNA sequence.    46  0.56  1
U43375|U43375.1 Caenorhabditis elegans cosmid K09C4.    46  0.56  1
AC122687|AC122687.2 Homo sapiens 12 BAC RP13-501J24 (Roswell Park Cancer Institute Human BAC Library) complete sequence.    38  1.2  2
AC116982|AC116982.2 Dictyostelium discoideum chromosome 2 map 3622643-3879522 strain AX4, complete sequence.    34  1.3  7
AC115599|AC115599.2 Dictyostelium discoideum chromosome 2 map 4229098-4354721 strain AX4, complete sequence.    36  1.4  6
AE016967|AE016967.1 Mycoplasma gallisepticum strain R section 1 of 4 of the complete genome.    42  1.5  6
dna update 2003. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q9Z311) RecName: Full=Trans-2-enoyl-CoA reductase, mitochondria...    99   8e-24
AF151821_1(AF151821|pid:none) Homo sapiens CGI-63 protein mRNA, ...   100   1e-23
BC111152_1(BC111152|pid:none) Bos taurus mitochondrial trans-2-e...    97   1e-23
CR761275_1(CR761275|pid:none) Xenopus tropicalis finished cDNA, ...   101   2e-23
CR456703_1(CR456703|pid:none) Homo sapiens full open reading fra...    99   2e-23
AL590729_2(AL590729|pid:none) Human DNA sequence from clone RP11...    99   3e-23
AK300889_1(AK300889|pid:none) Homo sapiens cDNA FLJ56723 complet...    96   2e-22
BT047630_1(BT047630|pid:none) Salmo salar clone ssal-evf-523-019...    92   7e-22
AC135230_7(AC135230|pid:none) Medicago truncatula clone mth2-23e...    85   8e-19
(Q8LCU7) RecName: Full=Probable trans-2-enoyl-CoA reductase, mit...    81   2e-17
protein update 2009. 6.29
PSORT 

 psg: 0.76  gvh: 0.41  alm: 0.46  top: 0.53  tms: 0.00  mit: 0.58  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 84.0 %: mitochondrial
	 12.0 %: cytoplasmic
	  4.0 %: nuclear

>> prediction for AFO280 is mit
5' end seq. ID AFO280F
5' end seq. 
>AFO280F.Seq
AATTTAATACAGCATATAATTCATTCATACATTTAATCAAACAATGAATTCTACTCGTAA
TATCATTTCATTAGTTCGTCGTTATTCAACTTCAAGAAGTGTTAAAATCGCTTCACATGG
TTCACCATCAACTGCTTTAAAGATTGAAAATGAAAATATTACAGATAAAATTTCAAATAA
AGATGTTTTAGTTGAAATGTTACATGCACCAATTAATCCAGCAGATTTAAATATTATTCA
AGGTACTTATGGTACAAATGTTCAAGTTGGAGGTGTAGCAGGTATGGAAGGTGTTGGTGT
TGTTAAAAAGGTTGGTAGTGGTGTTACAGGATTAAAAGAGAATGATTTAGTAGTACCATC
AATGAAACAACACTTTGGATCATGGAGAAGTAAAGGTGTTTGGAGTGAACAACAATTATT
TAAAGTACCATCAGACATTCCAACCGAATACTTATCCACCATTTCAATCAATCCAACCAC
TGCCTATTTGTTATTGAACGATTTCGTAAAGTTACAACAAGGCGATGTCATCATTCAAAA
CGCATCCAACTCAATGGTTGGTTTGTCAGTCATTCAATTGGCAAAGGCACGTGGTATCAA
AACCATCAATGTCATTCGTGACGGTTCAGAATTCGAA----------
Length of 5' end seq. 637
3' end seq. ID AFO280Z
3' end seq. 
>AFO280Z.Seq
----------ATGTCATCATTCGGNACNGCATCCANCTCAATGGTTGGTTTGTCAGTCAT
TCAATTGGCAAAGGCACGTGGTATCANANCCATCAATGTCATTCAGTGACGGTTCAGAAT
TCGAAGACAACGTCCAACGTCTCAAGCAGTTGGGTGG
Length of 3' end seq. 147
Connected seq. ID AFO280P
Connected seq. 
>AFO280P.Seq
AATTTAATACAGCATATAATTCATTCATACATTTAATCAAACAATGAATTCTACTCGTAA
TATCATTTCATTAGTTCGTCGTTATTCAACTTCAAGAAGTGTTAAAATCGCTTCACATGG
TTCACCATCAACTGCTTTAAAGATTGAAAATGAAAATATTACAGATAAAATTTCAAATAA
AGATGTTTTAGTTGAAATGTTACATGCACCAATTAATCCAGCAGATTTAAATATTATTCA
AGGTACTTATGGTACAAATGTTCAAGTTGGAGGTGTAGCAGGTATGGAAGGTGTTGGTGT
TGTTAAAAAGGTTGGTAGTGGTGTTACAGGATTAAAAGAGAATGATTTAGTAGTACCATC
AATGAAACAACACTTTGGATCATGGAGAAGTAAAGGTGTTTGGAGTGAACAACAATTATT
TAAAGTACCATCAGACATTCCAACCGAATACTTATCCACCATTTCAATCAATCCAACCAC
TGCCTATTTGTTATTGAACGATTTCGTAAAGTTACAACAAGGCGATGTCATCATTCAAAA
CGCATCCAACTCAATGGTTGGTTTGTCAGTCATTCAATTGGCAAAGGCACGTGGTATCAA
AACCATCAATGTCATTCGTGACGGTTCAGAATTCGAA----------ATGTCATCATTCG
GNACNGCATCCANCTCAATGGTTGGTTTGTCAGTCATTCAATTGGCAAAGGCACGTGGTA
TCANANCCATCAATGTCATTCAGTGACGGTTCAGAATTCGAAGACAACGTCCAACGTCTC
AAGCAGTTGGGTGG
Length of connected seq. 784
Full length Seq ID AFO280E
Full length Seq. 
>AFO280E.Seq
AATTTAATACAGCATATAATTCATTCATACATTTAATCAAACAATGAATTCTACTCGTAA
TATCATTTCATTAGTTCGTCGTTATTCAACTTCAAGAAGTGTTAAAATCGCTTCACATGG
TTCACCATCAACTGCTTTAAAGATTGAAAATGAAAATATTACAGATAAAATTTCAAATAA
AGATGTTTTAGTTGAAATGTTACATGCACCAATTAATCCAGCAGATTTAAATATTATTCA
AGGTACTTATGGTACAAATGTTCAAGTTGGAGGTGTAGCAGGTATGGAAGGTGTTGGTGT
TGTTAAAAAGGTTGGTAGTGGTGTTACAGGATTAAAAGAGAATGATTTAGTAGTACCATC
AATGAAACAACACTTTGGATCATGGAGAAGTAAAGGTGTTTGGAGTGAACAACAATTATT
TAAAGTACCATCAGACATTCCAACCGAATACTTATCCACCATTTCAATCAATCCAACCAC
TGCCTATTTGTTATTGAACGATTTCGTAAAGTTACAACAAGGCGATGTCATCATTCNNAA
CNGCATCCAACTCAATGGTTGGTTTGTCAGTCATTCAATTGGCAAAGGCACGTGGTATCA
AAACCATCAATGTCATTCAGTGACGGTTCAGAATTCGAAGACAACGTCCAACGTCTCAAG
CAGTTGGGTGG
Length of full length seq. 671