>AFO278 (AFO278Q) /CSM/AF/AFO2-D/AFO278Q.Seq.d/
XXXXXXXXXXGTAAANAAANNANNNGNCCTCNNAAAGTNTNATNCNAATGGTGTAGTTTC
NAATCATTTGCATGCAAATGTTAAGGTTGGCGATAAAGTTTTACTCTCCCCTCCAGNTGG
TGACTATGTAGTCGNCCAATTATCAAGCAATCCAATCCTTTTGGTTAGTGGTGGTGTTGG
TATCACTCCATTATTAAGTATGGCAAAGGCAACTTTAGCTAAACAACCAGAAAGAGAAGT
CACCTTTGTTCACTCTTCAAAGAACAAACAATATCAACCATTTGCAAATGAATTATCCCA
ATTGGAAAAATCAAATAAAGTAAAGGTATCAACTGTTCATTCTGAAACTGATGGTCAAAT
CACCAAAGATAAATTAGAAAAATTCATCAATCCTTCTCAAATTAAAGATACTAAAGTTTT
CATCTGTGGTCCAGTATCTTTTATGTCTGCAATTAATAAACAATTAATTGAATTAGGTTA
TCCAA

---XKXXXLXKXXXNGVVSNHLHANVKVGDKVLLSPPXGDYVVXQLSSNPILLVSGGVGI
TPLLSMAKATLAKQPEREVTFVHSSKNKQYQPFANELSQLEKSNKVKVSTVHSETDGQIT
KDKLEKFINPSQIKDTKVFICGPVSFMSAINKQLIELGYP

Frame A:
---*xxxxxsxsxxxmv*fxiicmqmlrlaikfysplqxvtm*sxnyqaiqsfwlvvvlv
slhy*vwqrql*lnnqkeksplftlqrtnninhlqmnypnwknqik*ryqlfilklmvks
pkin*knssillklkilkfssvvqyllclqlinn*ln*viq


Frame B:
---kxxxxpxkvxxxwcsfxsfackc*gwr*sftlpsxw*lcsrpiikqsnpfg*wwcwy
hsiikygkgnfs*ttrkrshlcslfkeqtistick*iipigkik*skgincsf*n*wsnh
qr*irkihqsfsn*ry*sfhlwssifyvcn**tin*irls


Frame C:
---XKXXXLXKXXXNGVVSNHLHANVKVGDKVLLSPPXGDYVVXQLSSNPILLVSGGVGI
TPLLSMAKATLAKQPEREVTFVHSSKNKQYQPFANELSQLEKSNKVKVSTVHSETDGQIT
KDKLEKFINPSQIKDTKVFICGPVSFMSAINKQLIELGYP

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VSH228 (VSH228Q) /CSM/VS/VSH2-B/VSH228Q.Seq.d/                        854   0.0
VSA808 (VSA808Q) /CSM/VS/VSA8-A/VSA808Q.Seq.d/                        854   0.0
VSA370 (VSA370Q) /CSM/VS/VSA3-C/VSA370Q.Seq.d/                        854   0.0
VFF225 (VFF225Q) /CSM/VF/VFF2-B/VFF225Q.Seq.d/                        854   0.0
SLA171 (SLA171Q) /CSM/SL/SLA1-C/SLA171Q.Seq.d/                        854   0.0
FCL-AA17 (FCL-AA17Q) /CSM/FCL/FCL-AA/FCL-AA17Q.Seq.d/                 854   0.0
AFO278 (AFO278Q) /CSM/AF/AFO2-D/AFO278Q.Seq.d/                        854   0.0
SLC715 (SLC715Q) /CSM/SL/SLC7-A/SLC715Q.Seq.d/                        846   0.0
VSI227 (VSI227Q) /CSM/VS/VSI2-B/VSI227Q.Seq.d/                        841   0.0
AFM604 (AFM604Q) /CSM/AF/AFM6-A/AFM604Q.Seq.d/                        841   0.0

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AB025583|AB025583.1 Dictyostelium discoideum DdFHa mRNA for flavohemoglobin, complete cds.   854  0.0  1
AL773569|AL773569.1 Homo sapiens chromosome 21 from PAC RPCI-1 247E2 map  21q22.2,D21S349-MX1.    32  0.61  6
BM093255|BM093255.1 saj06h02.y1 Gm-c1065 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1065-9939 5', mRNA sequence.    42  0.76  2
BE329911|BE329911.1 so71b06.y1 Gm-c1040 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1040-1068 5', mRNA sequence.    42  0.78  2
BI424033|BI424033.1 sah65a10.y1 Gm-c1049 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1049-3092 5', mRNA sequence.    42  0.81  2
BU548483|BU548483.1 GM880017A20D01 Gm-r1088 Glycine max cDNA clone Gm-r1088-6290 3', mRNA sequence.    42  1.0  2
BH493220|BH493220.1 BOHKH44TF BOHK Brassica oleracea genomic clone BOHKH44, DNA sequence.    44  1.6  1
Z33348|Z33348.1 M.capricolum DNA for CONTIG MCAAJ.                34  2.3  2
BE802815|BE802815.1 sr45a02.y1 Gm-c1051 Glycine max cDNA clone GENOME SYSTEMS CLONE ID: Gm-c1051-963 5', mRNA sequence.    40  2.6  2
AE001432|AE001432.1 Plasmodium falciparum chromosome 2, section 69 of 73 of the complete sequence.    36  3.6  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AB025584_1(AB025584|pid:none) Dictyostelium discoideum DdFHb mRN...   150   1e-35
AP009484_684(AP009484|pid:none) Macrococcus caseolyticus JCSC540...   103   3e-21
BX294154_248(BX294154|pid:none) Rhodopirellula baltica SH 1 comp...    98   8e-20
(Q7UIY1) RecName: Full=Flavohemoprotein; AltName: Full=Hemoglobi...    98   8e-20
CP000903_1350(CP000903|pid:none) Bacillus weihenstephanensis KBA...    96   3e-19
AM398681_1804(AM398681|pid:none) Flavobacterium psychrophilum JI...    96   5e-19
CP001186_1337(CP001186|pid:none) Bacillus cereus G9842, complete...    96   5e-19
CP000789_86(CP000789|pid:none) Vibrio harveyi ATCC BAA-1116 chro...    96   5e-19
CP000764_1120(CP000764|pid:none) Bacillus cereus subsp. cytotoxi...    95   9e-19
(Q81FW4) RecName: Full=Flavohemoprotein; AltName: Full=Hemoglobi...    94   1e-18

 psg: 0.78  gvh: 0.44  alm: 0.32  top: 0.60  tms: 0.07  mit: 0.24  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.09  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: nuclear
	 28.0 %: cytoplasmic
	 12.0 %: mitochondrial
	  8.0 %: Golgi
	  4.0 %: vesicles of secretory system

>> prediction for AFO278 is nuc

>AFO278Z.Seq
----------GTAAANAAANNANNNGNCCTCNNAAAGTNTNATNCNAATGGTGTAGTTTC
NAATCATTTGCATGCAAATGTTAAGGTTGGCGATAAAGTTTTACTCTCCCCTCCAGNTGG
TGACTATGTAGTCGNCCAATTATCAAGCAATCCAATCCTTTTGGTTAGTGGTGGTGTTGG
TATCACTCCATTATTAAGTATGGCAAAGGCAACTTTAGCTAAACAACCAGAAAGAGAAGT
CACCTTTGTTCACTCTTCAAAGAACAAACAATATCAACCATTTGCAAATGAATTATCCCA
ATTGGAAAAATCAAATAAAGTAAAGGTATCAACTGTTCATTCTGAAACTGATGGTCAAAT
CACCAAAGATAAATTAGAAAAATTCATCAATCCTTCTCAAATTAAAGATACTAAAGTTTT
CATCTGTGGTCCAGTATCTTTTATGTCTGCAATTAATAAACAATTAATTGAATTAGGTTA
TCCAA