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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI778
Library VH
(Link to library)
Clone ID VHI778
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12694-1
Original site URL
Representative seq. ID VHI778P
(Link to Original site)
Representative DNA sequence 
>VHI778 (VHI778Q) /CSM/VH/VHI7-D/VHI778Q.Seq.d/
AAATATATAAATCAACCATTATATTATATATATATATATAATTTTTTTTTAATATTATTT
TTTTTAAAAAAAAAGAATGATTAATTCTTTTAAAAAATTAATTATTCTTATTTCATTGGN
GATTATTTTACTTTCATCGAATAATATTTTCATAGATTCATTTAAAATTCCAGTTAATCA
AAAAAATGTTAAATCAAGNGGNGATTCATCATATCAAATAGGTGCCGGTATTTATGATAT
CACTGGNGCAAGCGCTGAAGTTAATTTAATGGGTTATGCTAATCCACTTCAAGTNGGNGC
AGGTATTCATTTTCGTCAAAGAGCACGTGCCTTTGTTTTTGTANATTCAAATGGTAATAG
ANCTGTATATGTTAGTNCTGATTCATGTATGATTTTCCAAGAAGTTAAAATTCATGTGGT
TGAATTGCTACAANATATTTTTGGACCNAATGTTTATXXXXXXXXXXGTAGAAGATTAAG
AAATAGTGTTAGAGAAATCATTGGGGAATCAATTGAAAATCCAATTGTTTTAATTGCTGG
TCTTTCCAATACTTATTCTGGTTATATAGCAACATTTGAAGAATATCAAGTTCAAAGATA
TGAGGGTGCATCAACTGTTTTTGGTCCACATACTCTTGGTTCATATATGCAAGAGTTTGG
AAAACTTGCACAATCAATTGTTGATGGAACAACAGTTCCTGCAGGTCCAACTCCAAGAAA
TTTAACAGGTCACACACTTTTCTTTTTACCACCAGTAATTGTAGATGCCGCACCAGATTT
TGATGATTTTGGTGAAGTTTCAATTGATGTCAATCTTAATTATTCAGTAAATGAAACAGT
ATCATGTGTTTTTTATGGTGGTAATCCTAGAAATGATTTTATGATTGAATCATCATTCTT
ATCAGTTGATTTATTGACAGGTACAGATCAATGGACAACAGTTTTAGATGATGGTGATTG
GGATACAAAATTCAAATGGAAAATGCACGATTTAGGTTTTTCTTTAATTACTATAGAATG
GGTTATTGCTCCAGATACAACACCAGGTACTTATAGAATAACTCATAGTGGTTTTGCTAA
AAAGAATCCTTTCTCTTCAAATTTAACTCCTTATCAAGGTATCTCAAGAAATTTTGTAGT
TCAATAAAGTACTTTATATTTTAGATTATAAATAAATAAAT
sequence update 2002.10.25
Translated Amino Acid sequence 
ni*inhyiiyiyiiff*yyff*KKRMINSFKKLIILISLXIILLSSNNIFIDSFKIPVNQ
KNVKSXGDSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVXSNGNR
XVYVSXDSCMIFQEVKIHVVELLQXIFGPNVY---

---RRLRNSVREIIGESIENPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLG
SYMQEFGKLAQSIVDGTTVPAGPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVNLN
YSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMHDLGF
SLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVVQ*stlyfrl*ink
Translated Amino Acid sequence (All Frames) 
Frame A:
kyinqplyyiyiynfflilfflkkknd*ff*kinysyfigdyftfie*yfhrfi*nss*s
kkc*ikxxfiisnrcryl*yhwxkr*s*fnglc*stssxxrysfsskstclcfcxfkw**
xcic*x*fmydfprs*nscg*iatxyfwtxcl---

---ved*eivlekslgnqlkiqlf*llvfpililvi*qhlknikfkdmrvhqlflvhill
vhickslenlhnqllmeqqflqvqlqei*qvthfsfyhq*l*mphqilmilvkfqlmsil
iiq*mkqyhvffmvvilemil*lnhhsyqliy*qvqingqqf*mmvigiqnsngkcti*v
fl*ll*nglllqiqhqvlie*livvllkrilslqi*llikvsqeil*fnkvlyildyk*i
n

Frame B:
ni*inhyiiyiyiiff*yyff*KKRMINSFKKLIILISLXIILLSSNNIFIDSFKIPVNQ
KNVKSXGDSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVXSNGNR
XVYVSXDSCMIFQEVKIHVVELLQXIFGPNVY---

---*kikk*c*rnhwgin*ksncfncwsfqylfwlysni*rissski*gcincfwstysw
fiyarvwktctinc*wnnsscrsnskkfnrshtflfttsncrcrtrf**fw*sfn*cqs*
lfsk*nsimcflww*s*k*fyd*iiilis*fidryrsmdnsfr*w*lgykiqmenarfrf
ffnyyrmgycsryntryl*nns*wfc*kesflfkfnslsrylkkfcssikyfif*iink*

Frame C:
iykstiilyiyi*fffniiffkkke*lillkn*lflfhwxlfyfhriifs*ihlkfqlik
kmlnqxxihhik*vpvfmislxqalkli*wvmlihfkxxqvfifvkehvplflxiqmvix
lymlvlihv*fskklkfmwlncyxifldxmf---

---RRLRNSVREIIGESIENPIVLIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLG
SYMQEFGKLAQSIVDGTTVPAGPTPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVNLN
YSVNETVSCVFYGGNPRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMHDLGF
SLITIEWVIAPDTTPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVVQ*stlyfrl*ink
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI778 (VHI778Q) /CSM/VH/VHI7-D/VHI778Q.Seq.d/                       2028   0.0
CHS327 (CHS327Q) /CSM/CH/CHS3-B/CHS327Q.Seq.d/                       1415   0.0
AFM442 (AFM442Q) /CSM/AF/AFM4-B/AFM442Q.Seq.d/                       1411   0.0
AHF544 (AHF544Q) /CSM/AH/AHF5-B/AHF544Q.Seq.d/                       1402   0.0
AHK614 (AHK614Q) /CSM/AH/AHK6-A/AHK614Q.Seq.d/                       1394   0.0
AHM195 (AHM195Q) /CSM/AH/AHM1-D/AHM195Q.Seq.d/                       1392   0.0
VHC444 (VHC444Q) /CSM/VH/VHC4-B/VHC444Q.Seq.d/                       1384   0.0
AHM667 (AHM667Q) /CSM/AH/AHM6-C/AHM667Q.Seq.d/                       1384   0.0
AFE563 (AFE563Q) /CSM/AF/AFE5-C/AFE563Q.Seq.d/                       1382   0.0
VHJ717 (VHJ717Q) /CSM/VH/VHJ7-A/VHJ717Q.Seq.d/                       1380   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442119) Dictyostelium discoideum cDNA clone:ddv48l19, 3' ...  1413   0.0    1
(BJ386453) Dictyostelium discoideum cDNA clone:ddc58f07, 3' ...  1413   0.0    1
(BJ346852) Dictyostelium discoideum cDNA clone:dda25d12, 3' ...  1411   0.0    1
(BJ355159) Dictyostelium discoideum cDNA clone:dda55m23, 3' ...  1390   0.0    1
(BJ437778) Dictyostelium discoideum cDNA clone:ddv35h12, 3' ...  1382   0.0    1
(BJ351182) Dictyostelium discoideum cDNA clone:dda42g11, 3' ...  1380   0.0    2
(BJ353985) Dictyostelium discoideum cDNA clone:dda52k04, 3' ...  1372   0.0    2
(BJ442479) Dictyostelium discoideum cDNA clone:ddv50b05, 3' ...  1350   0.0    2
(BJ355358) Dictyostelium discoideum cDNA clone:dda56e18, 3' ...  1350   0.0    2
(BJ339855) Dictyostelium discoideum cDNA clone:dda10m15, 3' ...  1350   0.0    2
dna update 2008.11.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55G11) RecName: Full=Neutral ceramidase B;          Short=N-CD...   464   e-129
U82513_1(U82513|pid:none) Dictyostelium discoideum random slug c...   350   4e-95
EU200974_1(EU200974|pid:none) Triticum aestivum neutral ceramida...   184   5e-45
CP000777_2353(CP000777|pid:none) Leptospira biflexa serovar Pato...   180   1e-43
(Q304B9) RecName: Full=Neutral ceramidase;          Short=N-CDas...   177   5e-43
AP002819_24(AP002819|pid:none) Oryza sativa Japonica Group genom...   177   5e-43
AK099625_1(AK099625|pid:none) Oryza sativa Japonica Group cDNA c...   177   5e-43
AC022464_28(AC022464|pid:none) Genomic sequence for Arabidopsis ...   176   2e-42
AB016885_17(AB016885|pid:none) Arabidopsis thaliana genomic DNA,...   169   1e-40
BT004471_1(BT004471|pid:none) Drosophila melanogaster SD07768 fu...   167   7e-40
protein update 2009. 7.14
PSORT 

 psg: 0.95  gvh: 0.62  alm: 0.27  top: 0.27  tms: 0.07  mit: 0.42  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: mitochondrial
	 24.0 %: Golgi
	 16.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for VHI778 is mit
5' end seq. ID VHI778F
5' end seq. 
>VHI778F.Seq
AAATATATAAATCAACCATTATATTATATATATATATATAATTTTTTTTTAATATTATTT
TTTTTAAAAAAAAAGAATGATTAATTCTTTTAAAAAATTAATTATTCTTATTTCATTGGN
GATTATTTTACTTTCATCGAATAATATTTTCATAGATTCATTTAAAATTCCAGTTAATCA
AAAAAATGTTAAATCAAGNGGNGATTCATCATATCAAATAGGTGCCGGTATTTATGATAT
CACTGGNGCAAGCGCTGAAGTTAATTTAATGGGTTATGCTAATCCACTTCAAGTNGGNGC
AGGTATTCATTTTCGTCAAAGAGCACGTGCCTTTGTTTTTGTANATTCAAATGGTAATAG
ANCTGTATATGTTAGTNCTGATTCATGTATGATTTTCCAAGAAGTTAAAATTCATGTGGT
TGAATTGCTACAANATATTTTTGGACCNAATGTTTATNNNNNNNNNN
Length of 5' end seq. 467
3' end seq. ID VHI778Z
3' end seq. 
>VHI778Z.Seq
NNNNNNNNNNGTAGAAGATTAAGAAATAGTGTTAGAGAAATCATTGGGGAATCAATTGAA
AATCCAATTGTTTTAATTGCTGGTCTTTCCAATACTTATTCTGGTTATATAGCAACATTT
GAAGAATATCAAGTTCAAAGATATGAGGGTGCATCAACTGTTTTTGGTCCACATACTCTT
GGTTCATATATGCAAGAGTTTGGAAAACTTGCACAATCAATTGTTGATGGAACAACAGTT
CCTGCAGGTCCAACTCCAAGAAATTTAACAGGTCACACACTTTTCTTTTTACCACCAGTA
ATTGTAGATGCCGCACCAGATTTTGATGATTTTGGTGAAGTTTCAATTGATGTCAATCTT
AATTATTCAGTAAATGAAACAGTATCATGTGTTTTTTATGGTGGTAATCCTAGAAATGAT
TTTATGATTGAATCATCATTCTTATCAGTTGATTTATTGACAGGTACAGATCAATGGACA
ACAGTTTTAGATGATGGTGATTGGGATACAAAATTCAAATGGAAAATGCACGATTTAGGT
TTTTCTTTAATTACTATAGAATGGGTTATTGCTCCAGATACAACACCAGGTACTTATAGA
ATAACTCATAGTGGTTTTGCTAAAAAGAATCCTTTCTCTTCAAATTTAACTCCTTATCAA
GGTATCTCAAGAAATTTTGTAGTTCAATAAAGTACTTTATATTTTAGATTATAAATAAAT
AAAT
Length of 3' end seq. 724
Connected seq. ID VHI778P
Connected seq. 
>VHI778P.Seq
AAATATATAAATCAACCATTATATTATATATATATATATAATTTTTTTTTAATATTATTT
TTTTTAAAAAAAAAGAATGATTAATTCTTTTAAAAAATTAATTATTCTTATTTCATTGGN
GATTATTTTACTTTCATCGAATAATATTTTCATAGATTCATTTAAAATTCCAGTTAATCA
AAAAAATGTTAAATCAAGNGGNGATTCATCATATCAAATAGGTGCCGGTATTTATGATAT
CACTGGNGCAAGCGCTGAAGTTAATTTAATGGGTTATGCTAATCCACTTCAAGTNGGNGC
AGGTATTCATTTTCGTCAAAGAGCACGTGCCTTTGTTTTTGTANATTCAAATGGTAATAG
ANCTGTATATGTTAGTNCTGATTCATGTATGATTTTCCAAGAAGTTAAAATTCATGTGGT
TGAATTGCTACAANATATTTTTGGACCNAATGTTTAT----------GTAGAAGATTAAG
AAATAGTGTTAGAGAAATCATTGGGGAATCAATTGAAAATCCAATTGTTTTAATTGCTGG
TCTTTCCAATACTTATTCTGGTTATATAGCAACATTTGAAGAATATCAAGTTCAAAGATA
TGAGGGTGCATCAACTGTTTTTGGTCCACATACTCTTGGTTCATATATGCAAGAGTTTGG
AAAACTTGCACAATCAATTGTTGATGGAACAACAGTTCCTGCAGGTCCAACTCCAAGAAA
TTTAACAGGTCACACACTTTTCTTTTTACCACCAGTAATTGTAGATGCCGCACCAGATTT
TGATGATTTTGGTGAAGTTTCAATTGATGTCAATCTTAATTATTCAGTAAATGAAACAGT
ATCATGTGTTTTTTATGGTGGTAATCCTAGAAATGATTTTATGATTGAATCATCATTCTT
ATCAGTTGATTTATTGACAGGTACAGATCAATGGACAACAGTTTTAGATGATGGTGATTG
GGATACAAAATTCAAATGGAAAATGCACGATTTAGGTTTTTCTTTAATTACTATAGAATG
GGTTATTGCTCCAGATACAACACCAGGTACTTATAGAATAACTCATAGTGGTTTTGCTAA
AAAGAATCCTTTCTCTTCAAATTTAACTCCTTATCAAGGTATCTCAAGAAATTTTGTAGT
TCAATAAAGTACTTTATATTTTAGATTATAAATAAATAAAT
Length of connected seq. 1171
Full length Seq ID -
Full length Seq. -
Length of full length seq. -