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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI769
Library VH
(Link to library)
Clone ID VHI769
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16260-1
Original site URL
Representative seq. ID VHI769P
(Link to Original site)
Representative DNA sequence 
>VHI769 (VHI769Q) /CSM/VH/VHI7-C/VHI769Q.Seq.d/
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTAXXX
XXXXXXXAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCC
CACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTT
GTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTA
GCCCCAGAAATCCCAGATCTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCAC
TTGAGTTTTCAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVG---

---AHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGL
APEIPDLTNSTKNSKQFKSHLSFQ
Translated Amino Acid sequence (All Frames) 
Frame A:
nsrvqkk*stkwlisqsikfvplwtvvkiseicllllmsitvkplyqil*skelvllpik
flvi*dichvvlmnksvvsplnlhlyhftskcqrktnyqlvvlhtnf*silsipqvtlis
vqk*llpsvllmvl*slsiaskvfaykpklyyvkpslnvsnqsysstksivsy*nsn*tl
kklislsvellnpsmsllv---

---kliyqy*npsvspliyvptlpvklshnvssiiglqlvlstkirkplksl*pperekv
*pqksqi*qiprktlnnlsrt*vfk

Frame B:
tleckkskvqng*fhnrsnschygps*kyqkyvcycscrsr*nhfirffnpkswyycr*s
fw*yeiyvmsc**trawyhh*ificitslrnakgrqitswlyftrifnqsyrfprsr*fq
frsnccppcy*wcfsryrlrrrclrtnrncitssrr*tyqtslirqqsrsflirtpikh*
rslslfp*sy*irqchcw---

---ssftsirilrfhr*ftfphcrssfptmclrslgfnwccqqr*esh*srfshqkekrf
sprnprsdkfhekl*ti*valefs

Frame C:
LSSAKKVKYKMVNFTIDQIRAIMDRRENIRNMSVIAHVDHGKTTLSDSLIQRAGIIADKV
SGDMRYMSCRADEQERGITIKSSSVSLHFEMPKEDKLPAGCTSHEFLINLIDSPGHVDFS
SEVTAALRVTDGALVVIDCVEGVCVQTETVLRQAVAERIKPVLFVNKVDRFLLELQLNTE
EAYLSFRRAIESVNVIVG---

---AHLPVLESFGFTADLRSHTAGQAFPQCVFDHWASIGVVNKDKKATEVALATRKRKGL
APEIPDLTNSTKNSKQFKSHLSFQ
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            1144   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1144   0.0
VHP874 (VHP874Q) /CSM/modify/h12/F/treated/VHP874Q.Seq.d/            1144   0.0
VHG874 (VHG874Q) /CSM/VH/VHG8-D/VHG874Q.Seq.d/                       1144   0.0
VHQ476 (VHQ476Q) /CSM/VH/VHQ4-D/VHQ476Q.Seq.d/                       1144   0.0
VHQ473 (VHQ473Q) /CSM/VH/VHQ4-D/VHQ473Q.Seq.d/                       1144   0.0
VHQ469 (VHQ469Q) /CSM/VH/VHQ4-C/VHQ469Q.Seq.d/                       1144   0.0
VHQ463 (VHQ463Q) /CSM/VH/VHQ4-C/VHQ463Q.Seq.d/                       1144   0.0
VHQ453 (VHQ453Q) /CSM/VH/VHQ4-C/VHQ453Q.Seq.d/                       1144   0.0
VHQ452 (VHQ452Q) /CSM/VH/VHQ4-C/VHQ452Q.Seq.d/                       1144   0.0
own update 2009. 4. 4
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ427897) Dictyostelium discoideum cDNA clone:ddv63p23, 5' ...  1144   0.0    1
(BJ427892) Dictyostelium discoideum cDNA clone:ddv63o22, 5' ...  1144   0.0    1
(BJ427891) Dictyostelium discoideum cDNA clone:ddv63o21, 5' ...  1144   0.0    1
(BJ427888) Dictyostelium discoideum cDNA clone:ddv63n23, 5' ...  1144   0.0    1
(BJ427875) Dictyostelium discoideum cDNA clone:ddv63k24, 5' ...  1144   0.0    1
(BJ427874) Dictyostelium discoideum cDNA clone:ddv63k23, 5' ...  1144   0.0    1
(BJ427873) Dictyostelium discoideum cDNA clone:ddv63k22, 5' ...  1144   0.0    1
(BJ427872) Dictyostelium discoideum cDNA clone:ddv63k21, 5' ...  1144   0.0    1
(BJ427858) Dictyostelium discoideum cDNA clone:ddv63h20, 5' ...  1144   0.0    1
(BJ427848) Dictyostelium discoideum cDNA clone:ddv63f21, 5' ...  1144   0.0    1
dna update 2008.11.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AM270011_29(AM270011|pid:none) Aspergillus niger contig An02c016...   264   2e-69
AF248644_1(AF248644|pid:none) Filobasidiella neoformans var. neo...   258   1e-67
AF083031_87(AF083031|pid:none) Guillardia theta nucleomorph chro...   256   5e-67
(Q6FYA7) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   5e-67
AY026074_1(AY026074|pid:none) Monosiga brevicollis elongation fa...   256   7e-67
BC089730_1(BC089730|pid:none) Xenopus tropicalis MGC108369 prote...   256   9e-67
(Q874B9) RecName: Full=Elongation factor 2;          Short=EF-2;...   256   9e-67
DQ357217_1(DQ357217|pid:none) Leishmania braziliensis elongation...   254   2e-66
AM494972_26(AM494972|pid:none) Leishmania braziliensis chromosom...   254   2e-66
CT005272_19(CT005272|pid:none) Leishmania major strain Friedlin,...   254   3e-66
protein update 2009. 7.14
PSORT 

 psg: 0.71  gvh: 0.38  alm: 0.34  top: 0.53  tms: 0.00  mit: 0.30  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: vesicles of secretory system

>> prediction for VHI769 is cyt
5' end seq. ID VHI769F
5' end seq. 
>VHI769F.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTANNN
NNNNNNN
Length of 5' end seq. 607
3' end seq. ID VHI769Z
3' end seq. 
>VHI769Z.Seq
NNNNNNNNNNAAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGT
TCCCACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGT
GTTGTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGT
TTAGCCCCAGAAATCCCAGATCTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCG
CACTTGAGTTTTCAAA
Length of 3' end seq. 256
Connected seq. ID VHI769P
Connected seq. 
>VHI769P.Seq
AACTCTCGAGTGCAAAAAAAGTAAAGTACAAAATGGTTAATTTCACAATCGATCAAATTC
GTGCCATTATGGACCGTCGTGAAAATATCAGAAATATGTCTGTTATTGCTCATGTCGATC
ACGGTAAAACCACTTTATCAGATTCTTTAATCCAAAGAGCTGGTATTATTGCCGATAAAG
TTTCTGGTGATATGAGATATATGTCATGTCGTGCTGATGAACAAGAGCGTGGTATCACCA
TTAAATCTTCATCTGTATCACTTCACTTCGAAATGCCAAAGGAAGACAAATTACCAGCTG
GTTGTACTTCACACGAATTTTTAATCAATCTTATCGATTCCCCAGGTCACGTTGATTTCA
GTTCAGAAGTAACTGCTGCCCTCCGTGTTACTGATGGTGCTTTAGTCGTTATCGATTGCG
TCGAAGGTGTTTGCGTACAAACCGAAACTGTATTACGTCAAGCCGTCGCTGAACGTATCA
AACCAGTCTTATTCGTCAACAAAGTCGATCGTTTCTTATTAGAACTCCAATTAAACACTG
AAGAAGCTTATCTCTCTTTCCGTAGAGCTATTGAATCCGTCAATGTCATTGTTGGTA---
-------AAGCTCATTTACCAGTATTAGAATCCTTCGGTTTCACCGCTGATTTACGTTCC
CACACTGCCGGTCAAGCTTTCCCACAATGTGTCTTCGATCATTGGGCTTCAATTGGTGTT
GTCAACAAAGATAAGAAAGCCACTGAAGTCGCTTTAGCCACCAGAAAGAGAAAAGGTTTA
GCCCCAGAAATCCCAGATCTGACAAATTCCACGAAAAACTCTAAACAATTTAAGTCGCAC
TTGAGTTTTCAAA
Length of connected seq. 843
Full length Seq ID -
Full length Seq. -
Length of full length seq. -