>AFO274 (AFO274Q) /CSM/AF/AFO2-D/AFO274Q.Seq.d/
ACTGTTGGCCTATGGCATATAATANAGGAAGAGGTAGAGGAAGAACAAAAAGAAGAAGGA
GCAACTATTTATTTATTTATTTAAACAACAACAACAAAAACAATAGCAATAACAATAGCA
ATAGCAACTACTATAGAAAAAAAAAAXXXXXXXXXX

CWPMAYNXGRGRGRTKRRRSNYLFIYLNNNNKNNSNNNSNSNYYRKK---

Frame A:
tvglwhiixeeveeeqkeegatiylfi*ttttktiaitiaiattiekk---


Frame B:
llaygi*xrkr*rknkkkkeqlfiylfkqqqqkq*q*q*q*qll*kkk---


Frame C:
CWPMAYNXGRGRGRTKRRRSNYLFIYLNNNNKNNSNNNSNSNYYRKK---

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO274 (AFO274Q) /CSM/AF/AFO2-D/AFO274Q.Seq.d/                        157   6e-38
VSH168 (VSH168Q) /CSM/VS/VSH1-C/VSH168Q.Seq.d/                        127   5e-29
AFN840 (AFN840Q) /CSM/AF/AFN8-B/AFN840Q.Seq.d/                        127   5e-29
AFI660 (AFI660Q) /CSM/AF/AFI6-C/AFI660Q.Seq.d/                        127   5e-29
AFB342 (AFB342Q) /CSM/AF/AFB3-B/AFB342Q.Seq.d/                        127   5e-29
CFJ684 (CFJ684Q) /CSM/CF/CFJ6-D/CFJ684Q.Seq.d/                        121   3e-27
SFK501 (SFK501Q) /CSM/SF/SFK5-A/SFK501Q.Seq.d/                        115   2e-25
AFC706 (AFC706Q) /CSM/AF/AFC7-A/AFC706Q.Seq.d/                         92   3e-18
VSD184 (VSD184Q) /CSM/VS/VSD1-D/VSD184Q.Seq.d/                         80   1e-14
VSJ479 (VSJ479Q) /CSM/VS/VSJ4-D/VSJ479Q.Seq.d/                         76   2e-13

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AC012149|AC012149.11 Homo sapiens chromosome 3 clone RP11-119E13, WORKING DRAFT SEQUENCE, 9 unordered pieces.    46  3e-05  2
AC005005|AC005005.1 Homo sapiens PAC clone RP3-412A9 from 22, complete sequence.    48  9e-05  2
AC026612|AC026612.3 Homo sapiens chromosome 3 clone RP11-717C3 map 3, WORKING DRAFT SEQUENCE, 50 unordered pieces.    46  1e-04  2
AC106532|AC106532.4 Rattus norvegicus clone CH230-136K8, WORKING DRAFT SEQUENCE, 1 ordered piece.    50  2e-04  2
AC097423|AC097423.6 Rattus norvegicus clone CH230-45B14, *** SEQUENCING IN PROGRESS ***, 5 unordered pieces.    50  2e-04  2
AC068766|AC068766.13 Homo sapiens chromosome 3 clone RP11-84J20, WORKING DRAFT SEQUENCE, 58 unordered pieces.    50  2e-04  2
AC140203|AC140203.1 Mus musculus chromosome UNK clone RP24-334J15, WORKING DRAFT SEQUENCE, 7 unordered pieces.    48  4e-04  2
AC134852|AC134852.2 Mus musculus chromosome UNK clone RP24-248B6, WORKING DRAFT SEQUENCE, 11 unordered pieces.    48  5e-04  2
AC069393|AC069393.8 Homo sapiens chromosome 7 clone RP11-585K18, WORKING DRAFT SEQUENCE, 14 unordered pieces.    42  0.001  2
AC133552|AC133552.3 Homo sapiens chromosome 16 clone RP11-266L9, complete sequence.    46  0.001  2

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 67,514,869

 psg: 0.19  gvh: 0.34  alm: 0.79  top: 0.53  tms: 0.00  mit: 0.62  mip: 0.00
 nuc: 0.16  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: nuclear
	 32.0 %: cytoplasmic
	 16.0 %: mitochondrial
	  4.0 %: cytoskeletal

>> prediction for AFO274 is nuc

>AFO274F.Seq
ACTGTTGGCCTATGGCATATAATANAGGAAGAGGTAGAGGAAGAACAAAAAGAAGAAGGA
GCAACTATTTATTTATTTATTTAAACAACAACAACAAAAACAATAGCAATAACAATAGCA
ATAGCAACTACTATAGAAAAAAAAAA----------