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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI738
Library VH
(Link to library)
Clone ID VHI738
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig -
Original site URL
Representative seq. ID VHI738P
(Link to Original site)
Representative DNA sequence 
>VHI738 (VHI738Q) /CSM/VH/VHI7-B/VHI738Q.Seq.d/
GTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTG
ATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTATTG
CAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAACGTT
TAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATG
CTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAACATT
GGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCAAAG
GTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAAAATGAAAG
AAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGTTCCAGCTT
ATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAAATTAAATG
TTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAGXXXXXXXXX
XTGTAAGAAAACTCCAACCCTTCTCTACATACTCTGATAATCAACCAGGTGTATTAATTC
AAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAAC
TTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTG
ATGCAAATGGTATCTTAAATGTATTAGCTGAAGATAAATCAACTGGTAACAAACAAAAGA
TTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTG
ATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATA
AATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCA
AAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGT
TAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAG
CAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTG
GTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAAT
TAGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAA
TAAGTTAGTAAAATCAATAAAGCTTCTCATATATAGTACACA
sequence update 2002.10.25
Translated Amino Acid sequence 
*nksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTERL
IGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKG
ETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNV
QRIINEPTAAAIAYGL---

---VRKLQPFSTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTF
DVDANGILNVLAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVES
KNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKA
LEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*
kiis**nq*sfsyivh
Translated Amino Acid sequence (All Frames) 
Frame A:
vkirvnik*lnikkchqlvli*vqhihvlvfgkmivlkllqmikvieplhlmlhlliqnv
*lvmlqriklq*iqpiqylmlnv*lvvnlvtkkfkvi*nighsklfqrmvishifklnsk
vklkfshqkkslqwys*k*kkplklilvkpliml*lpfqlismivnvkqpkmlvqfqn*m
fnvllmnqllllllmv*---

---ckktptllyil**strcinssl*r*tcydqr**fig*i*tfwyststkrctts*syi
*c*ckwylkcis*r*inw*qtkdynyq**rssfkrrn*kngc*creiqttr*ttkrsc*i
ke*igklcihc*kfn*r*kscsqnfrfr*iny*i*n*kctqmvriksnrrkg*i*r*des
lrsscqsnhe*tlsrrryatrwwyarwyvk*ftkefkqqsrrirlteffrgkhlyqidfl
knnklvksiklliyst

Frame B:
lk*e*ilnn*ilknvinwy*frynifmcwclak*sc*nyck*sr**nhsilcciy*yrtf
nw*cckesscnesnqysi*c*tfnws*i**qrssk*yetlaiqsyskgw**atyss*iqr
*n*sfltrrnlfngtlknernr*slsw*nh**ccnyrsslfq**stssnqrcwynfkikc
styy**tncccyclwf---

---VRKLQPFSTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQVEVTF
DVDANGILNVLAEDKSTGNKQKITITNDKGRLSKEEIEKMVADAEKFKQQDEQQKDRVES
KNKLENYAFTVKNSIKDEKVAAKISDSDKSTIESETESVLKWLESNQTAEKDEYEDKMKA
LEAVVNPIMSKLYQEGGMPQGGGMPGGMSNDSPKSSNNKVDELD*rnfseenisik*is*
kiis**nq*sfsyivh

Frame C:
*nksky*iiky*KMSSIGIDLGTTYSCVGVWQNDRVEIIANDQGNRTTPSYVAFTDTERL
IGDAAKNQVAMNPTNTVFDAKRLIGRKFSDKEVQSDMKHWPFKVIPKDGDKPHIQVEFKG
ETKVFSPEEISSMVLLKMKETAEAYLGKTINNAVITVPAYFNDSQRQATKDAGTISKLNV
QRIINEPTAAAIAYGL---

---*ensnpslhtliinqvy*fkfmkvnvl*pkiiiywvnlnflvfhqhqevyhklklhl
mlmqmvs*my*lkinqlvtnkrlqlpmikvvfqkkklkkwllmqrnsnnkmnnkkivlnq
rinwkimhsllkiqlkmkklqpkfqiqinqllnlklkvysng*nqikpqkrmnmkir*kp
*kqlsiqs*vnsikkevchkvvvcqvvcqmihqrvqttk*tn*idgifqrktslsnrflk
k**vskinkashi*yt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI738 (VHI738Q) /CSM/VH/VHI7-B/VHI738Q.Seq.d/                       2555   0.0
VHN681 (VHN681Q) /CSM/VH/VHN6-D/VHN681Q.Seq.d/                       2533   0.0
VHP719 (VHP719Q) /CSM/VH/VHP7-A/VHP719Q.Seq.d/                       1495   0.0
CHF190 (CHF190Q) /CSM/CH/CHF1-D/CHF190Q.Seq.d/                       1495   0.0
VHK721 (VHK721Q) /CSM/VH/VHK7-A/VHK721Q.Seq.d/                       1493   0.0
VHD443 (VHD443Q) /CSM/VH/VHD4-B/VHD443Q.Seq.d/                       1493   0.0
VHA371 (VHA371Q) /CSM/VH/VHA3-C/VHA371Q.Seq.d/                       1493   0.0
VHH695 (VHH695Q) /CSM/VH/VHH6-D/VHH695Q.Seq.d/                       1491   0.0
CHI295 (CHI295Q) /CSM/CH/CHI2-D/CHI295Q.Seq.d/                       1491   0.0
AHL860 (AHL860Q) /CSM/AH/AHL8-C/AHL860Q.Seq.d/                       1487   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441966) Dictyostelium discoideum cDNA clone:ddv48l09, 3' ...  1509   0.0    1
(BJ446228) Dictyostelium discoideum cDNA clone:ddv62f05, 3' ...  1495   0.0    1
(BJ378765) Dictyostelium discoideum cDNA clone:ddc32c23, 3' ...  1495   0.0    1
(BJ443174) Dictyostelium discoideum cDNA clone:ddv52j05, 3' ...  1493   0.0    1
(BJ438567) Dictyostelium discoideum cDNA clone:ddv37f12, 3' ...  1493   0.0    1
(BJ436609) Dictyostelium discoideum cDNA clone:ddv31n17, 3' ...  1493   0.0    1
(BJ441509) Dictyostelium discoideum cDNA clone:ddv46m24, 3' ...  1491   0.0    1
(BJ380671) Dictyostelium discoideum cDNA clone:ddc38m24, 3' ...  1491   0.0    1
(BJ445191) Dictyostelium discoideum cDNA clone:ddv58a22, 3' ...  1487   0.0    1
(BJ349049) Dictyostelium discoideum cDNA clone:dda35p05, 3' ...  1487   0.0    1
dna update 2008.11.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AK097824_1(AK097824|pid:none) Homo sapiens cDNA FLJ40505 fis, cl...   368   e-100
(P36415) RecName: Full=Heat shock cognate 70 kDa protein 1;     ...   363   6e-99
S37394(S37394) dnaK-type molecular chaperone hsc70 - slime mold ...   363   6e-99
(Q557E0) RecName: Full=Heat shock cognate 70 kDa protein 2;     ...   338   3e-91
AF025951_1(AF025951|pid:none) Dictyostelium discoideum heat-shoc...   338   3e-91
(Q54BE0) RecName: Full=Heat shock cognate 70 kDa protein 3;           320   1e-85
U71151_1(U71151|pid:none) Pneumocystis carinii heat shock protei...   315   2e-84
AY495382_1(AY495382|pid:none) Trypanosoma rangeli isolate Tre he...   315   2e-84
U80967_1(U80967|pid:none) Pneumocystis carinii f. sp. carinii he...   315   2e-84
(P11145) RecName: Full=Heat shock 70 kDa protein 4; AltName: Ful...   313   7e-84
protein update 2009. 7.14
PSORT 

 psg: 0.87  gvh: 0.35  alm: 0.45  top: 0.27  tms: 0.00  mit: 0.19  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 52.0 %: cytoplasmic
	 32.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHI738 is cyt
5' end seq. ID VHI738F
5' end seq. 
>VHI738F.Seq
GTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTG
ATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTATTG
CAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAACGTT
TAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATG
CTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAACATT
GGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCAAAG
GTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAAAATGAAAG
AAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGTTCCAGCTT
ATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAAATTAAATG
TTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAGNNNNNNNNN
N
Length of 5' end seq. 601
3' end seq. ID VHI738Z
3' end seq. 
>VHI738Z.Seq
NNNNNNNNNNTGTAAGAAAACTCCAACCCTTCTCTACATACTCTGATAATCAACCAGGTG
TATTAATTCAAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTA
AATTTGAACTTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACAT
TTGATGTTGATGCAAATGGTATCTTAAATGTATTAGCTGAAGATAAATCAACTGGTAACA
AACAAAAGATTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAA
TGGTTGCTGATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAAT
CAAAGAATAAATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAG
TTGCAGCCAAAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTAC
TCAAATGGTTAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAG
CCTTAGAAGCAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCAC
AAGGTGGTGGTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAG
TAGACGAATTAGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTT
AAAAAATAATAAGTTAGTAAAATCAATAAAGCTTCTCATATATAGTACACA
Length of 3' end seq. 771
Connected seq. ID VHI738P
Connected seq. 
>VHI738P.Seq
GTTAAAATAAGAGTAAATATTAAATAATTAAATATTAAAAAATGTCATCAATTGGTATTG
ATTTAGGTACAACATATTCATGTGTTGGTGTTTGGCAAAATGATCGTGTTGAAATTATTG
CAAATGATCAAGGTAATAGAACCACTCCATCTTATGTTGCATTTACTGATACAGAACGTT
TAATTGGTGATGCTGCAAAGAATCAAGTTGCAATGAATCCAACCAATACAGTATTTGATG
CTAAACGTTTAATTGGTCGTAAATTTAGTGACAAAGAAGTTCAAAGTGATATGAAACATT
GGCCATTCAAAGTTATTCCAAAGGATGGTGATAAGCCACATATTCAAGTTGAATTCAAAG
GTGAAACTAAAGTTTTCTCACCAGAAGAAATCTCTTCAATGGTACTCTTAAAAATGAAAG
AAACCGCTGAAGCTTATCTTGGTAAAACCATTAATAATGCTGTAATTACCGTTCCAGCTT
ATTTCAATGATAGTCAACGTCAAGCAACCAAAGATGCTGGTACAATTTCAAAATTAAATG
TTCAACGTATTATTAATGAACCAACTGCTGCTGCTATTGCTTATGGTTTAG---------
-TGTAAGAAAACTCCAACCCTTCTCTACATACTCTGATAATCAACCAGGTGTATTAATTC
AAGTTTATGAAGGTGAACGTGCTATGACCAAAGATAATAATTTATTGGGTAAATTTGAAC
TTTCTGGTATTCCACCAGCACCAAGAGGTGTACCACAAGTTGAAGTTACATTTGATGTTG
ATGCAAATGGTATCTTAAATGTATTAGCTGAAGATAAATCAACTGGTAACAAACAAAAGA
TTACAATTACCAATGATAAAGGTCGTCTTTCAAAAGAAGAAATTGAAAAAATGGTTGCTG
ATGCAGAGAAATTCAAACAACAAGATGAACAACAAAAAGATCGTGTTGAATCAAAGAATA
AATTGGAAAATTATGCATTCACTGTTAAAAATTCAATTAAAGATGAAAAAGTTGCAGCCA
AAATTTCAGATTCAGATAAATCAACTATTGAATCTGAAACTGAAAGTGTACTCAAATGGT
TAGAATCAAATCAAACCGCAGAAAAGGATGAATATGAAGATAAGATGAAAGCCTTAGAAG
CAGTTGTCAATCCAATCATGAGTAAACTCTATCAAGAAGGAGGTATGCCACAAGGTGGTG
GTATGCCAGGTGGTATGTCAAATGATTCACCAAAGAGTTCAAACAACAAAGTAGACGAAT
TAGATTGACGGAATTTTTCAGAGGAAAACATCTCTATCAAATAGATTTCTTAAAAAATAA
TAAGTTAGTAAAATCAATAAAGCTTCTCATATATAGTACACA
Length of connected seq. 1352
Full length Seq ID -
Full length Seq. -
Length of full length seq. -