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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI729
Library VH
(Link to library)
Clone ID VHI729
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11503-1
Original site URL
Representative seq. ID VHI729E
(Link to Original site)
Representative DNA sequence 
>VHI729 (VHI729Q) /CSM/VH/VHI7-B/VHI729Q.Seq.d/
ATCAAAAAAAACAGTTTTCTTTGTAAATTTATTAATATATGATTAGAAAATCAAATAATT
TTTCAATTTTGTTTGCAATATTTTTAAAAATTGTTTTTGTGGTTTCAGCGCCGCTATGCC
CAAATTCAACGATTTTATTAAATTACAACATCCTAACGGTTTATAATTCATCAGAAGGAT
GTGGTTTTAATAACGAGCCGATATGCACATCACTCAAAGATGCTGTTAGCAGAGCATTTT
TATTAATATCAAATAATAGTCGTGTTTGTATTGGTATCATTGGTAATATAAATGTAACAT
CAGAACAAATTACATTGGGTAATTATTGTGGTGCCTTGTGGATCACTTCAGAAAATATCA
ATAATGAAAATAATAATTATNCAATAATCGATGGATCAAGCTCAACCCAACCATTTCTGA
CATATGAACCAGAAGAAGAAAATAACCAAACTCAATCATGTTCAAGATGGCTCTTCTTTC
AATATATGAATTTCACAAGTTGGGATTGGAAATAATTCAGATGTGAATTTATCAAAAAAA
AGCACTAAATTAAATAACATTTATTGTGGTTGTGAAGAATGTAAATATTTTTCTTATATA
TGGTTCTCAATTTATTCCCATCGTTAACCACACTTGCATTGATCCGACACTGACACCGAC
ACCAACAACCTCAACAACATCTACTACATTTGCTTCCACAACATCAACCACAACTACTTC
AACAACATCAAACGCATCAGCTGGAACAACATCAACTACTTCAGCTGCAACAACACCAAC
CACATCAGCTTTAACAACATCAACTACAACTACAACATCAACTACAACAACTATAACTAC
CTCAACTACATTACCATCAACATCAACAACAACAAGTCCAATTGATAGTAAGACTAGGAA
TATTATCATTGTTACAGTTATTTTGGGCTTGATCTATAGCATGTGGTAGACTATGAACGT
CAATTGAAGATTATGCGGATAGGTAACTCAACTTCANATCTTATATCGTTTT
sequence update 2002.10.27
Translated Amino Acid sequence 
qkkqfsl*iy*YMIRKSNNFSILFAIFLKIVFVVSAPLCPNSTILLNYNILTVYNSSEGC
GFNNEPICTSLKDAVSRAFLLISNNSRVCIGIIGNINVTSEQITLGNYCGALWITSENIN
NENNNYXIIDGSSSTQPFLTYEPEEENNQTQSCSRWLFFQYMNFTSWDWK*frcefikkk
h*ik*hllwl*rm*ifflymvlnlfpslttlalirh*hrhqqpqqhllhllpqhqpqllq
qhqthqleqhqllqlqqhqphql*qhqlqlqhqlqql*lpqlhyhqhqqqqvqlivrlgi
lsllqlfwa*siacgrl*tsiedyadr*lnfxsyiv
Translated Amino Acid sequence (All Frames) 
Frame A:
ikknsflckfini*lenqiifqfclqyf*klflwfqrryaqiqrfy*itts*rfiihqkd
vvlitsryahhskmllaehfy*yqiivvfvlvslvi*m*hqnklhwviivvpcgslqkis
imkiiixq*smdqaqpnhf*hmnqkkkitklnhvqdgssfni*isqvgignnsdvnlskk
stklnniycgceeckyfsyiwfsiyshr*phlh*sdtdtdtnnlnniyyicfhninhnyf
nnikriswnninyfscnntnhisfnninynyninynnynylnyitininnnksn***d*e
yyhcysyfgldl*hvvdyerqlkimrignstsxlisf


Frame B:
skktvffvnlliyd*kik*ffnfvcnifkncfcgfsaampkfndfiklqhpngl*firrm
wf**radmhitqrcc*qsifinik**sclywyhw*ykcnirtnyig*llwclvdhfrkyq
**k**lxnnrwiklnptisdi*trrrk*pnsimfkmallsiyefhklgleiiqm*iyqkk
aln*itfivvvknvnifliygsqfipivnhtcidptltptpttsttsttfasttstttts
ttsnasagttsttsaattpttsalttstttttsttttittsttlpststttspidsktrn
iiivtvilgliysmw*tmnvn*rlcg*vtqlxilyrf


Frame C:
qkkqfsl*iy*YMIRKSNNFSILFAIFLKIVFVVSAPLCPNSTILLNYNILTVYNSSEGC
GFNNEPICTSLKDAVSRAFLLISNNSRVCIGIIGNINVTSEQITLGNYCGALWITSENIN
NENNNYXIIDGSSSTQPFLTYEPEEENNQTQSCSRWLFFQYMNFTSWDWK*frcefikkk
h*ik*hllwl*rm*ifflymvlnlfpslttlalirh*hrhqqpqqhllhllpqhqpqllq
qhqthqleqhqllqlqqhqphql*qhqlqlqhqlqql*lpqlhyhqhqqqqvqlivrlgi
lsllqlfwa*siacgrl*tsiedyadr*lnfxsyiv
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI729 (VHI729Q) /CSM/VH/VHI7-B/VHI729Q.Seq.d/                       1255   0.0
AHK839 (AHK839Q) /CSM/AH/AHK8-B/AHK839Q.Seq.d/                       1239   0.0
AHF753 (AHF753Q) /CSM/AH/AHF7-C/AHF753Q.Seq.d/                       1239   0.0
VHE495 (VHE495Q) /CSM/VH/VHE4-D/VHE495Q.Seq.d/                       1233   0.0
SHC657 (SHC657Q) /CSM/SH/SHC6-C/SHC657Q.Seq.d/                       1233   0.0
AHK570 (AHK570Q) /CSM/AH/AHK5-C/AHK570Q.Seq.d/                       1233   0.0
AHA119 (AHA119Q) /CSM/AH/AHA1-A/AHA119Q.Seq.d/                       1233   0.0
SHD533 (SHD533Q) /CSM/SH/SHD5-B/SHD533Q.Seq.d/                       1231   0.0
AHG336 (AHG336Q) /CSM/AH/AHG3-B/AHG336Q.Seq.d/                       1231   0.0
AHH235 (AHH235Q) /CSM/AH/AHH2-B/AHH235Q.Seq.d/                       1227   0.0
own update 2004.12.25
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ355279) Dictyostelium discoideum cDNA clone:dda56e12, 3' ...   355   0.0    7
(BJ354136) Dictyostelium discoideum cDNA clone:dda52k17, 3' ...   355   0.0    7
(BJ351996) Dictyostelium discoideum cDNA clone:dda45e10, 3' ...   355   0.0    7
(BJ349210) Dictyostelium discoideum cDNA clone:dda35b24, 3' ...   355   0.0    7
(BJ403573) Dictyostelium discoideum cDNA clone:dds25f18, 3' ...   343   0.0    6
(BJ446682) Dictyostelium discoideum cDNA clone:ddv63c14, 3' ...   355   0.0    6
(BJ354074) Dictyostelium discoideum cDNA clone:dda52n10, 3' ...   355   0.0    7
(BJ356493) Dictyostelium discoideum cDNA clone:dda60a18, 3' ...   355   0.0    7
(BJ441955) Dictyostelium discoideum cDNA clone:ddv48j07, 3' ...   448   0.0    4
(BJ352678) Dictyostelium discoideum cDNA clone:dda47e16, 3' ...   355   0.0    6
dna update 2008.11.15
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AF020283_1(AF020283|pid:none) Dictyostelium discoideum DG2044 ge...    62   3e-08
CP000766_763(CP000766|pid:none) Rickettsia rickettsii str. Iowa,...    37   1.4
CP000084_528(CP000084|pid:none) Candidatus Pelagibacter ubique H...    36   1.9
AE006914_723(AE006914|pid:none) Rickettsia conorii str. Malish 7...    35   5.4
CP001227_577(CP001227|pid:none) Rickettsia peacockii str. Rustic...    35   5.4
protein update 2009. 7.14
PSORT 

 psg: 0.99  gvh: 0.48  alm: 0.21  top: 0.27  tms: 0.07  mit: 0.47  mip: 0.02
 nuc: 0.00  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.45  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 48.0 %: mitochondrial
	 20.0 %: Golgi
	 12.0 %: nuclear
	  8.0 %: endoplasmic reticulum
	  4.0 %: cytoplasmic
	  4.0 %: vacuolar
	  4.0 %: extracellular, including cell wall

>> prediction for VHI729 is mit
5' end seq. ID VHI729F
5' end seq. 
>VHI729F.Seq
ATCAAAAAAAACAGTTTTCTTTGTAAATTTATTAATATATGATTAGAAAATCAAATAATT
TTTCAATTTTGTTTGCAATATTTTTAAAAATTGTTTTTGTGGTTTCAGCGCCGCTATGCC
CAAATTCAACGATTTTATTAAATTACAACATCCTAACGGTTTATAATTCATCAGAAGGAT
GTGGTTTTAATAACGAGCCGATATGCACATCACTCAAAGATGCTGTTAGCAGAGCATTTT
TATTAATATCAAATAATAGTCGTGTTTGTATTGGTATCATTGGTAATATAAATGTAACAT
CAGAACAAATTACATTGGGTAATTATTGTGGTGCCTTGTGGATCACTTCAGAAAATATCA
ATAATGAAAATAATAATTATACAATAATCGATGGATCAAGCTCAACCCANCCNTNNNNNN
NNNN
Length of 5' end seq. 424
3' end seq. ID VHI729Z
3' end seq. 
>VHI729Z.Seq
NNNNNNNNNNGATCACTTCAGAAAATATCAATAATGAAAATAATAATTATCCAATAATCG
AGGATCAAGCTCAACCCAACCATTTCTGACATATGAACCAGAAGAAGAAAATAACCAAAC
TCAATCATGTTCAAGATGGCTCTTCTTTCAATATATGAATTTCACAAGTTGGGATTGGAA
ATAATTCAGATGTGAATTTATCAAAAAAAAGCACTAAATTAAATAACATTTATTGTGGTT
GTGAAGAATGTAAATATTTTTCTTATATATGGTTCTCAATTTATTCCCATCGTTAACCAC
ACTTGCATTGATCCGACACTGACACCGACACCAACAACCTCAACAACATCTACTACATTT
GCTTCCACAACATCAACCACAACTACTTCAACAACATCAAACGCATCAGCTGGAACAACA
TCAACTACTTCAGCTGCAACAACACCAACCACATCAGCTTTAACAACATCAACTACAACT
ACAACATCAACTACAACAACTATAACTACCTCAACTACATTACCATCAACATCAACAACA
ACAAGTCCAATTGATAGTAAGACTAGGAATATTATCATTGTTACAGTTATTTTGGGCTTG
ATCTATAGCATGTGGTAGACTATGAACGTCAATTGAAGATTATGCGGATAGGTAACTCAA
CTTCANATCTTATATCGTTTT
Length of 3' end seq. 681
Connected seq. ID VHI729P
Connected seq. 
>VHI729P.Seq
ATCAAAAAAAACAGTTTTCTTTGTAAATTTATTAATATATGATTAGAAAATCAAATAATT
TTTCAATTTTGTTTGCAATATTTTTAAAAATTGTTTTTGTGGTTTCAGCGCCGCTATGCC
CAAATTCAACGATTTTATTAAATTACAACATCCTAACGGTTTATAATTCATCAGAAGGAT
GTGGTTTTAATAACGAGCCGATATGCACATCACTCAAAGATGCTGTTAGCAGAGCATTTT
TATTAATATCAAATAATAGTCGTGTTTGTATTGGTATCATTGGTAATATAAATGTAACAT
CAGAACAAATTACATTGGGTAATTATTGTGGTGCCTTGTGGATCACTTCAGAAAATATCA
ATAATGAAAATAATAATTATACAATAATCGATGGATCAAGCTCAACCCANCCNT------
----GATCACTTCAGAAAATATCAATAATGAAAATAATAATTATCCAATAATCGAGGATC
AAGCTCAACCCAACCATTTCTGACATATGAACCAGAAGAAGAAAATAACCAAACTCAATC
ATGTTCAAGATGGCTCTTCTTTCAATATATGAATTTCACAAGTTGGGATTGGAAATAATT
CAGATGTGAATTTATCAAAAAAAAGCACTAAATTAAATAACATTTATTGTGGTTGTGAAG
AATGTAAATATTTTTCTTATATATGGTTCTCAATTTATTCCCATCGTTAACCACACTTGC
ATTGATCCGACACTGACACCGACACCAACAACCTCAACAACATCTACTACATTTGCTTCC
ACAACATCAACCACAACTACTTCAACAACATCAAACGCATCAGCTGGAACAACATCAACT
ACTTCAGCTGCAACAACACCAACCACATCAGCTTTAACAACATCAACTACAACTACAACA
TCAACTACAACAACTATAACTACCTCAACTACATTACCATCAACATCAACAACAACAAGT
CCAATTGATAGTAAGACTAGGAATATTATCATTGTTACAGTTATTTTGGGCTTGATCTAT
AGCATGTGGTAGACTATGAACGTCAATTGAAGATTATGCGGATAGGTAACTCAACTTCAN
ATCTTATATCGTTTT
Length of connected seq. 1085
Full length Seq ID VHI729E
Full length Seq. 
>VHI729E.Seq
ATCAAAAAAAACAGTTTTCTTTGTAAATTTATTAATATATGATTAGAAAATCAAATAATT
TTTCAATTTTGTTTGCAATATTTTTAAAAATTGTTTTTGTGGTTTCAGCGCCGCTATGCC
CAAATTCAACGATTTTATTAAATTACAACATCCTAACGGTTTATAATTCATCAGAAGGAT
GTGGTTTTAATAACGAGCCGATATGCACATCACTCAAAGATGCTGTTAGCAGAGCATTTT
TATTAATATCAAATAATAGTCGTGTTTGTATTGGTATCATTGGTAATATAAATGTAACAT
CAGAACAAATTACATTGGGTAATTATTGTGGTGCCTTGTGGATCACTTCAGAAAATATCA
ATAATGAAAATAATAATTATNCAATAATCGATGGATCAAGCTCAACCCAACCATTTCTGA
CATATGAACCAGAAGAAGAAAATAACCAAACTCAATCATGTTCAAGATGGCTCTTCTTTC
AATATATGAATTTCACAAGTTGGGATTGGAAATAATTCAGATGTGAATTTATCAAAAAAA
AGCACTAAATTAAATAACATTTATTGTGGTTGTGAAGAATGTAAATATTTTTCTTATATA
TGGTTCTCAATTTATTCCCATCGTTAACCACACTTGCATTGATCCGACACTGACACCGAC
ACCAACAACCTCAACAACATCTACTACATTTGCTTCCACAACATCAACCACAACTACTTC
AACAACATCAAACGCATCAGCTGGAACAACATCAACTACTTCAGCTGCAACAACACCAAC
CACATCAGCTTTAACAACATCAACTACAACTACAACATCAACTACAACAACTATAACTAC
CTCAACTACATTACCATCAACATCAACAACAACAAGTCCAATTGATAGTAAGACTAGGAA
TATTATCATTGTTACAGTTATTTTGGGCTTGATCTATAGCATGTGGTAGACTATGAACGT
CAATTGAAGATTATGCGGATAGGTAACTCAACTTCANATCTTATATCGTTTT
Length of full length seq. 1012