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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI681
Library VH
(Link to library)
Clone ID VHI681
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15693-1
Original site URL
Representative seq. ID VHI681P
(Link to Original site)
Representative DNA sequence 
>VHI681 (VHI681Q) /CSM/VH/VHI6-D/VHI681Q.Seq.d/
ATAGATATTTTGGAAGGAGAGAGAAAATATAAAAGAAGAAAAAAAATGGATGTTAATCAA
ATTAGAAAAACTTTTATCGATTTTTTTAGAGAGAAATGTGAACATACATTTGTTCCATCA
TCAGCAGTAATTCCACATGATGATCCAACTTTATTATTTGCAAATGCAGGTATGAATCAA
TTTAAACCAATCTTTTTAGGACAAGTTAATCCAAAATCAGAACAAGCAAAATTGAAGAGA
GCAGTCAATAGTCAAAAATGTATTCGTGCAGGTGGTAAACATAATGATTTGGATGATGTA
GGTAAGGATACTTATCATCACACCTTCTTTGAGATGTTGGGTAATTGGTCATTTGGCAAT
TACTTTAAGAAGGAGGCAATCACTTGGGCATGGGAATTATTGACAGAGGTATACAAATTA
GATAAAGAACGTTTATATGTCACTTACTTTAGAGGTGACCCAGAGAAAGGATTGGAAGCA
GATCTCGAAGCAAAGAACCTTTGGTTGCAATATTTACCAGAGGAACGTGTGCTCCCATTC
GGTATGAAAGAGAACTTTTGGGAGATGGGTGACCAAGGTCCATGTGGTCCATXXXXXXXX
XXGTGGTGGTACACATTTGTCAAACACTAAACAAGCTGAACTCTTCACCATTACCTCTGA
AGAAACTTTGGGTGCTGGTGTACGTCGTATCGTCGCTGTCACTGGTTCTGAAGCTGCCTC
TGCTATAGAACTCAATAAAGAATTGGAAGTCAGATTCAATAATGCCCTCAAATTATCAGG
TTCAGAACTTGCCAAGGAAATCGTTTCCTTATTAGATCTTCTCAAGGTTGTCACCATTTC
AGCAAGTGTTCGTATGAATTTGGTTGAAACTTTGAAGGAAGTTCAAGCACTCCAAAGAAA
ACAAGTCAAAGAACAAGAAACCATCTTGGCTCAACAAGCTCAAACCTATTTAGAGAAAAC
CTCTGAAGAATTGGCTAAATCTCAACCAAAGGTTTTCGTAGATTTAGTAAACTTCAATTC
AAATACTCCTTTAATCACTGAAACCATTAAAAAAATTCAAACTAAATCACCAATGACTGC
AATCATGTTAATTAGTCCAGATGAAGAAAAAGGTAAAGTTACTTGCATTGGTATCGTTCC
AAAAGATTCTGAAATCTCAAAAACTTTAACTGCAAATGCTTGGGTTGTAAAGGTTACCGA
AGTTTTAGGTGGTAAAGGTGGTGGTAAAGTTGATGTTGCTCAAGGTGTTGGTTCTAAATT
AGATAAAATCGATGAAGCTATTCTCGTTTCAAGAGAATTTGCAAATGCAAACNATTCAAA
TTATCTTTATAAAAAA
sequence update 2002.10.25
Translated Amino Acid sequence 
IDILEGERKYKRRKKMDVNQIRKTFIDFFREKCEHTFVPSSAVIPHDDPTLLFANAGMNQ
FKPIFLGQVNPKSEQAKLKRAVNSQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGN
YFKKEAITWAWELLTEVYKLDKERLYVTYFRGDPEKGLEADLEAKNLWLQYLPEERVLPF
GMKENFWEMGDQGPCGP---

---GGTHLSNTKQAELFTITSEETLGAGVRRIVAVTGSEAASAIELNKELEVRFNNALKL
SGSELAKEIVSLLDLLKVVTISASVRMNLVETLKEVQALQRKQVKEQETILAQQAQTYLE
KTSEELAKSQPKVFVDLVNFNSNTPLITETIKKIQTKSPMTAIMLISPDEEKGKVTCIGI
VPKDSEISKTLTANAWVVKVTEVLGGKGGGKVDVAQGVGSKLDKIDEAILVSREFANANX
SNYLYKK
Translated Amino Acid sequence (All Frames) 
Frame A:
IDILEGERKYKRRKKMDVNQIRKTFIDFFREKCEHTFVPSSAVIPHDDPTLLFANAGMNQ
FKPIFLGQVNPKSEQAKLKRAVNSQKCIRAGGKHNDLDDVGKDTYHHTFFEMLGNWSFGN
YFKKEAITWAWELLTEVYKLDKERLYVTYFRGDPEKGLEADLEAKNLWLQYLPEERVLPF
GMKENFWEMGDQGPCGP---

---vvvhicqtlnklnssplplkklwvlvyvvsslslvlklpll*nsiknwksdsimpsn
yqvqnlprksfpy*ifsrlspfqqvfv*iwlkl*rkfkhskenksknkkpswlnklkpi*
rkplknwlnlnqrfs*i**tsiqill*slkplkkfklnhq*lqsc*lvqmkkkvkllalv
sfqkilksqkl*lqmlgl*rlpkf*vvkvvvklmllkvlvln*iksmklfsfqenlqmqt
iqiifik

Frame B:
*ifwkerenikeekkwmliklekllsiflernvnihlfhhqq*fhmmiqlyylqmqv*in
lnqsf*dkliqnqnkqn*reqsivknvfvqvvnimiwmm*vriliitpslrcwvighlai
tlrrrqslghgny*qrytn*iknvymsltlevtqrkdwkqiskqrtfgcniyqrnvcshs
v*krtfgrwvtkvhvvh---

---wwytfvkh*ts*tlhhyl*rnfgcwctsyrrchwf*sclcyrtq*rigsqiq*cpqi
irfrtcqgnrflirssqgchhfskcsyefg*nfegssstpkktsqrtrnhlgstssnlfr
enl*rig*istkgfrrfsklqfkysfnh*nh*knsn*itndcnhvn*sr*rkr*sylhwy
rskrf*nlknfnckclgckgyrsfrw*rww*s*ccsrcwf*ir*nr*sysrfkrickckx
fklsl*k

Frame C:
ryfgrreki*kkkkngc*sn*knfyrff*rem*tyicsiissnst**snfiickcryesi
*tnlfrts*skirtskieessq*skmyscrw*t**fg*cr*gylsshll*dvg*lviwql
l*eggnhlgmgiidrgiqir*rtfichll*r*prerigsrsrskeplvaiftrgtcapir
yerellgdg*prsmws---

---GGTHLSNTKQAELFTITSEETLGAGVRRIVAVTGSEAASAIELNKELEVRFNNALKL
SGSELAKEIVSLLDLLKVVTISASVRMNLVETLKEVQALQRKQVKEQETILAQQAQTYLE
KTSEELAKSQPKVFVDLVNFNSNTPLITETIKKIQTKSPMTAIMLISPDEEKGKVTCIGI
VPKDSEISKTLTANAWVVKVTEVLGGKGGGKVDVAQGVGSKLDKIDEAILVSREFANANX
SNYLYKK
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI681 (VHI681Q) /CSM/VH/VHI6-D/VHI681Q.Seq.d/                       2452   0.0
VHD392 (VHD392Q) /CSM/VH/VHD3-D/VHD392Q.Seq.d/                       2367   0.0
VHK630 (VHK630Q) /CSM/VH/VHK6-B/VHK630Q.Seq.d/                       1392   0.0
SHG234 (SHG234Q) /CSM/SH/SHG2-B/SHG234Q.Seq.d/                       1390   0.0
SHA383 (SHA383Q) /CSM/SH/SHA3-D/SHA383Q.Seq.d/                       1388   0.0
AHN845 (AHN845Q) /CSM/AH/AHN8-B/AHN845Q.Seq.d/                       1374   0.0
SHL816 (SHL816Q) /CSM/SH/SHL8-A/SHL816Q.Seq.d/                       1298   0.0
AHB873 (AHB873Q) /CSM/AH/AHB8-D/AHB873Q.Seq.d/                       1273   0.0
AHC193 (AHC193Q) /CSM/AH/AHC1-D/AHC193Q.Seq.d/                       1271   0.0
SHA215 (SHA215Q) /CSM/SH/SHA2-A/SHA215Q.Seq.d/                       1261   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ423300) Dictyostelium discoideum cDNA clone:ddv48a22, 5' ...  1001   0.0    3
(BJ419982) Dictyostelium discoideum cDNA clone:ddv37h23, 5' ...  1001   0.0    2
(BJ397108) Dictyostelium discoideum cDNA clone:dds45h06, 5' ...  1001   0.0    3
(BJ332266) Dictyostelium discoideum cDNA clone:dda38c18, 5' ...  1001   0.0    3
(BJ330331) Dictyostelium discoideum cDNA clone:dda31n02, 5' ...  1001   0.0    2
(AF188717) Dictyostelium discoideum alanyl-tRNA synthetase (...   993   0.0    3
(BJ391666) Dictyostelium discoideum cDNA clone:dds24c19, 5' ...   993   0.0    3
(BJ335055) Dictyostelium discoideum cDNA clone:dda48c21, 5' ...   991   0.0    3
(BJ331551) Dictyostelium discoideum cDNA clone:dda35i23, 5' ...   944   0.0    3
(BJ442064) Dictyostelium discoideum cDNA clone:ddv48a22, 3' ...   882   0.0    2
dna update 2008.11.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54Y20) RecName: Full=Alanyl-tRNA synthetase;          EC=6.1.1...   390   e-107
CP000497_274(CP000497|pid:none) Pichia stipitis CBS 6054 chromos...   292   2e-77
BX842632_19(BX842632|pid:none) Neurospora crassa DNA linkage gro...   292   2e-77
CP001330_129(CP001330|pid:none) Micromonas sp. RCC299 chromosome...   292   2e-77
CR954204_497(CR954204|pid:none) Ostreococcus tauri strain OTTH05...   288   2e-76
(P36428) RecName: Full=Alanyl-tRNA synthetase, mitochondrial;   ...   283   8e-75
AC007980_17(AC007980|pid:none) Arabidopsis thaliana chromosome I...   283   8e-75
AM502240_136(AM502240|pid:none) Leishmania infantum chromosome 22.    283   1e-74
(P21894) RecName: Full=Alanyl-tRNA synthetase, cytoplasmic;     ...   282   2e-74
CT005261_152(CT005261|pid:none) Leishmania major strain Friedlin...   281   4e-74
protein update 2009. 7.14
PSORT 

 psg: 0.23  gvh: 0.30  alm: 0.31  top: 0.67  tms: 0.07  mit: 0.19  mip: 0.00
 nuc: 0.08  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.06  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 1.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: nuclear
	 20.0 %: mitochondrial
	 20.0 %: cytoplasmic
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum
	  4.0 %: peroxisomal

>> prediction for VHI681 is nuc
5' end seq. ID VHI681F
5' end seq. 
>VHI681F.Seq
ATAGATATTTTGGAAGGAGAGAGAAAATATAAAAGAAGAAAAAAAATGGATGTTAATCAA
ATTAGAAAAACTTTTATCGATTTTTTTAGAGAGAAATGTGAACATACATTTGTTCCATCA
TCAGCAGTAATTCCACATGATGATCCAACTTTATTATTTGCAAATGCAGGTATGAATCAA
TTTAAACCAATCTTTTTAGGACAAGTTAATCCAAAATCAGAACAAGCAAAATTGAAGAGA
GCAGTCAATAGTCAAAAATGTATTCGTGCAGGTGGTAAACATAATGATTTGGATGATGTA
GGTAAGGATACTTATCATCACACCTTCTTTGAGATGTTGGGTAATTGGTCATTTGGCAAT
TACTTTAAGAAGGAGGCAATCACTTGGGCATGGGAATTATTGACAGAGGTATACAAATTA
GATAAAGAACGTTTATATGTCACTTACTTTAGAGGTGACCCAGAGAAAGGATTGGAAGCA
GATCTCGAAGCAAAGAACCTTTGGTTGCAATATTTACCAGAGGAACGTGTGCTCCCATTC
GGTATGAAAGAGAACTTTTGGGAGATGGGTGACCAAGGTCCATGTGGTCCATNNNNNNNN
NN
Length of 5' end seq. 602
3' end seq. ID VHI681Z
3' end seq. 
>VHI681Z.Seq
NNNNNNNNNNGTGGTGGTACACATTTGTCAAACACTAAACAAGCTGAACTCTTCACCATT
ACCTCTGAAGAAACTTTGGGTGCTGGTGTACGTCGTATCGTCGCTGTCACTGGTTCTGAA
GCTGCCTCTGCTATAGAACTCAATAAAGAATTGGAAGTCAGATTCAATAATGCCCTCAAA
TTATCAGGTTCAGAACTTGCCAAGGAAATCGTTTCCTTATTAGATCTTCTCAAGGTTGTC
ACCATTTCAGCAAGTGTTCGTATGAATTTGGTTGAAACTTTGAAGGAAGTTCAAGCACTC
CAAAGAAAACAAGTCAAAGAACAAGAAACCATCTTGGCTCAACAAGCTCAAACCTATTTA
GAGAAAACCTCTGAAGAATTGGCTAAATCTCAACCAAAGGTTTTCGTAGATTTAGTAAAC
TTCAATTCAAATACTCCTTTAATCACTGAAACCATTAAAAAAATTCAAACTAAATCACCA
ATGACTGCAATCATGTTAATTAGTCCAGATGAAGAAAAAGGTAAAGTTACTTGCATTGGT
ATCGTTCCAAAAGATTCTGAAATCTCAAAAACTTTAACTGCAAATGCTTGGGTTGTAAAG
GTTACCGAAGTTTTAGGTGGTAAAGGTGGTGGTAAAGTTGATGTTGCTCAAGGTGTTGGT
TCTAAATTAGATAAAATCGATGAAGCTATTCTCGTTTCAAGAGAATTTGCAAATGCAAAC
NATTCAAATTATCTTTATAAAAAA
Length of 3' end seq. 744
Connected seq. ID VHI681P
Connected seq. 
>VHI681P.Seq
ATAGATATTTTGGAAGGAGAGAGAAAATATAAAAGAAGAAAAAAAATGGATGTTAATCAA
ATTAGAAAAACTTTTATCGATTTTTTTAGAGAGAAATGTGAACATACATTTGTTCCATCA
TCAGCAGTAATTCCACATGATGATCCAACTTTATTATTTGCAAATGCAGGTATGAATCAA
TTTAAACCAATCTTTTTAGGACAAGTTAATCCAAAATCAGAACAAGCAAAATTGAAGAGA
GCAGTCAATAGTCAAAAATGTATTCGTGCAGGTGGTAAACATAATGATTTGGATGATGTA
GGTAAGGATACTTATCATCACACCTTCTTTGAGATGTTGGGTAATTGGTCATTTGGCAAT
TACTTTAAGAAGGAGGCAATCACTTGGGCATGGGAATTATTGACAGAGGTATACAAATTA
GATAAAGAACGTTTATATGTCACTTACTTTAGAGGTGACCCAGAGAAAGGATTGGAAGCA
GATCTCGAAGCAAAGAACCTTTGGTTGCAATATTTACCAGAGGAACGTGTGCTCCCATTC
GGTATGAAAGAGAACTTTTGGGAGATGGGTGACCAAGGTCCATGTGGTCCAT--------
--GTGGTGGTACACATTTGTCAAACACTAAACAAGCTGAACTCTTCACCATTACCTCTGA
AGAAACTTTGGGTGCTGGTGTACGTCGTATCGTCGCTGTCACTGGTTCTGAAGCTGCCTC
TGCTATAGAACTCAATAAAGAATTGGAAGTCAGATTCAATAATGCCCTCAAATTATCAGG
TTCAGAACTTGCCAAGGAAATCGTTTCCTTATTAGATCTTCTCAAGGTTGTCACCATTTC
AGCAAGTGTTCGTATGAATTTGGTTGAAACTTTGAAGGAAGTTCAAGCACTCCAAAGAAA
ACAAGTCAAAGAACAAGAAACCATCTTGGCTCAACAAGCTCAAACCTATTTAGAGAAAAC
CTCTGAAGAATTGGCTAAATCTCAACCAAAGGTTTTCGTAGATTTAGTAAACTTCAATTC
AAATACTCCTTTAATCACTGAAACCATTAAAAAAATTCAAACTAAATCACCAATGACTGC
AATCATGTTAATTAGTCCAGATGAAGAAAAAGGTAAAGTTACTTGCATTGGTATCGTTCC
AAAAGATTCTGAAATCTCAAAAACTTTAACTGCAAATGCTTGGGTTGTAAAGGTTACCGA
AGTTTTAGGTGGTAAAGGTGGTGGTAAAGTTGATGTTGCTCAAGGTGTTGGTTCTAAATT
AGATAAAATCGATGAAGCTATTCTCGTTTCAAGAGAATTTGCAAATGCAAACNATTCAAA
TTATCTTTATAAAAAA
Length of connected seq. 1326
Full length Seq ID -
Full length Seq. -
Length of full length seq. -