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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI656
Library VH
(Link to library)
Clone ID VHI656
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U11181-1
Original site URL
Representative seq. ID VHI656P
(Link to Original site)
Representative DNA sequence 
>VHI656 (VHI656Q) /CSM/VH/VHI6-C/VHI656Q.Seq.d/
GAGACATAAAAGATGGAAAGTTTAAGTGAACAAGAAATAAAGTTTAGAGAAGATACAACT
AAAGTTTACAGGGAGTTATTATCAATTCCAACAGTCAACAACGCATTTTTTACAAACAAT
GATGAAAGTAATGAAAATCAAACATCAGCATCAATCATTGTATCTCAAATTGATATCACC
AATAAGAAAACTAAATTTTATATGTCACAAAAAACAATTTTAAAAGATTCATCATCATCA
TCAAAGAAAAACCAAGTAATTTCATCAGCAATTCAACAAGAATTAGCAACTTCAATCATA
TCAGTATCACCATCACATAAGAAAGTTTTAACAATTAAAGAGAACCCTTCAACTGAATTC
GAATTCTCTTTTGATATTACTGATGGTTCACATTTAATTACTTCAATTACTTCAAAAGAT
ATTCATAAAAAAATTTTAAATGATGAATGGTTTGGTAGTTTTTCATGGTCACCATGTGAG
AAATATGTAGCATTTATTGCAGATTCAAAATTAAATGTAACAGGATTTTTTGATAAGGAA
CCAAAAGATAAAGCTATTGGTGATCAATTTGTATTTAGAGAXXXXXXXXXXAATTTAGAT
GTTGGTTATGTTTCAACAATCACTTATTTACTCTCATTGGGTTATAATATTATAATTCCA
AATTATAGAGGTTCAACAGGTTATGGTAAAGATTTCGTAGATTGTTTACCAGGTTATATT
GGTGATTTGGATGTTGACGATTGTGTTCAAGCAATTAATTACACACTCGATAAAATCGAT
AATACAATCGATAGAAATAAAATTGGTGTTATTGGTGGTAGTCATGGTGGTTTCTTATCA
GCACATCTAAGTAGATTCCCAATTGTCAAAACTTCAATCATGAGAAATCCTGTAATCGAT
ATTCCATCAATGTCAACCTTATCCGATATTCCAGATTGGTGTTTCTTTGAAGCTGGTATT
AATCTCTCTGATCCAACCTCTCAATATCATACATTACCATCAATGGAAGAAATTGAAAAA
ATGAGAAAATGTTCACCAAGTTTCCATATTGAAAACATCAAAATTCCATCATTATTAGCT
TTAGGTGATTCAGATCTTCGTGTTCCACCTTCTCAAGGTTTATTATATTATCGTATGTTA
AGAGAAAGAGATGTACCAACAAAATGTTTAATGTATCCAAAAACTGGTCATAGTCTTGAT
TCAATTGATGCTCGTTTAGATCAATGGGTTAATATTTCATTATGGTTAAAAAAATATTTA
AATTAAAATTTTTAAAAAAAATTACAACAATTACACCACCACCACCACCACTACCACCAC
CATATGGTAAAATAATATTATTAAATAAAAACATTTTATCTTNCTTTNTTATA
sequence update 2002.10.25
Translated Amino Acid sequence 
et*KMESLSEQEIKFREDTTKVYRELLSIPTVNNAFFTNNDESNENQTSASIIVSQIDIT
NKKTKFYMSQKTILKDSSSSSKKNQVISSAIQQELATSIISVSPSHKKVLTIKENPSTEF
EFSFDITDGSHLITSITSKDIHKKILNDEWFGSFSWSPCEKYVAFIADSKLNVTGFFDKE
PKDKAIGDQFVFR---

---NLDVGYVSTITYLLSLGYNIIIPNYRGSTGYGKDFVDCLPGYIGDLDVDDCVQAINY
TLDKIDNTIDRNKIGVIGGSHGGFLSAHLSRFPIVKTSIMRNPVIDIPSMSTLSDIPDWC
FFEAGINLSDPTSQYHTLPSMEEIEKMRKCSPSFHIENIKIPSLLALGDSDLRVPPSQGL
LYYRMLRERDVPTKCLMYPKTGHSLDSIDARLDQWVNISLWLKKYLN*nf*kklqqlhhh
hhhyhhhmvk*yy*iktfylxxl
Translated Amino Acid sequence (All Frames) 
Frame A:
et*KMESLSEQEIKFREDTTKVYRELLSIPTVNNAFFTNNDESNENQTSASIIVSQIDIT
NKKTKFYMSQKTILKDSSSSSKKNQVISSAIQQELATSIISVSPSHKKVLTIKENPSTEF
EFSFDITDGSHLITSITSKDIHKKILNDEWFGSFSWSPCEKYVAFIADSKLNVTGFFDKE
PKDKAIGDQFVFR---

---NLDVGYVSTITYLLSLGYNIIIPNYRGSTGYGKDFVDCLPGYIGDLDVDDCVQAINY
TLDKIDNTIDRNKIGVIGGSHGGFLSAHLSRFPIVKTSIMRNPVIDIPSMSTLSDIPDWC
FFEAGINLSDPTSQYHTLPSMEEIEKMRKCSPSFHIENIKIPSLLALGDSDLRVPPSQGL
LYYRMLRERDVPTKCLMYPKTGHSLDSIDARLDQWVNISLWLKKYLN*nf*kklqqlhhh
hhhyhhhmvk*yy*iktfylxxl

Frame B:
rhkrwkv*vnkk*slekiqlkftgsyyqfqqstthflqtmmkvmkikhqhqslylklisp
irklnfichkkqf*kihhhhqrktk*fhqqfnkn*qlqsyqyhhhirkf*qlkrtlqlns
nsllillmvhi*llqllqkifikkf*mmnglvvfhghhvrnm*hllqiqn*m*qdflirn
qkikllvinlyle---

---i*mlvmfqqsliyshwviil*fqiievqqvmvkis*ivyqvilviwmltivfkqlit
hsiksiiqsieiklvllvvvmvvsyqhi*vdsqlsklqs*eil*sifhqcqpypifqigv
slklvlisliqplniihyhqwkklkk*envhqvsilktskfhhy*l*viqifvfhllkvy
yiivc*ekemyqqnv*ciqklvivliqlmlv*inglifhyg*kni*ikifkknynnyttt
ttttttiw*nniik*khfixlxy

Frame C:
dikdgkfk*trnkv*rryn*slqgviinsnsqqrifykq**k**ksnisinhcisn*yhq
*en*ilyvtknnfkrfiiiikekpsnfisnstrisnfnhisitit*esfnn*repfn*ir
ilf*yy*wftfnyfnyfkrys*knfk**mvw*ffmvtm*eicsiycrfkikcnrif**gt
kr*syw*sici*r---

---frcwlcfnnhlftligl*yynskl*rfnrlw*rfrrlftrlyw*fgc*rlcssn*lh
tr*nr*ynr*k*nwcyww*swwflistsk*ipncqnfnhekscnrysinvnlirysrlvf
l*swy*sl*snlsisyitingrn*knekmftkfpy*khqnsiiisfr*frsscstfsrfi
ilsyvkrkrctnkmfnvsknws*s*fn*csfrsmg*yfimvkkifklkflkkittitppp
pplpppygkiillnknilsxfxi
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI656 (VHI656Q) /CSM/VH/VHI6-C/VHI656Q.Seq.d/                       2369   0.0
VHO634 (VHO634Q) /CSM/VH/VHO6-B/VHO634Q.Seq.d/                       1298   0.0
VHO522 (VHO522Q) /CSM/VH/VHO5-A/VHO522Q.Seq.d/                       1298   0.0
VHB485 (VHB485Q) /CSM/VH/VHB4-D/VHB485Q.Seq.d/                       1298   0.0
VHB191 (VHB191Q) /CSM/VH/VHB1-D/VHB191Q.Seq.d/                       1298   0.0
AHQ746 (AHQ746Q) /CSM/AH/AHQ7-B/AHQ746Q.Seq.d/                       1292   0.0
CHI527 (CHI527Q) /CSM/CH/CHI5-B/CHI527Q.Seq.d/                       1291   0.0
CHQ849 (CHQ849Q) /CSM/CH/CHQ8-C/CHQ849Q.Seq.d/                       1285   0.0
VHP838 (VHP838Q) /CSM/VH/VHP8-B/VHP838Q.Seq.d/                       1283   0.0
VHA708 (VHA708Q) /CSM/VH/VHA7-A/VHA708Q.Seq.d/                       1279   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445675) Dictyostelium discoideum cDNA clone:ddv60c10, 3' ...  1296   0.0    1
(BJ445636) Dictyostelium discoideum cDNA clone:ddv60k05, 3' ...  1296   0.0    1
(BJ442051) Dictyostelium discoideum cDNA clone:ddv48o14, 3' ...  1296   0.0    1
(BJ437311) Dictyostelium discoideum cDNA clone:ddv33j22, 3' ...  1296   0.0    1
(BJ437292) Dictyostelium discoideum cDNA clone:ddv33e23, 3' ...  1296   0.0    1
(BJ357697) Dictyostelium discoideum cDNA clone:dda64l11, 3' ...  1291   0.0    1
(BJ380778) Dictyostelium discoideum cDNA clone:ddc39e07, 3' ...  1289   0.0    1
(BJ385598) Dictyostelium discoideum cDNA clone:ddc55b14, 3' ...  1283   0.0    1
(BJ446337) Dictyostelium discoideum cDNA clone:ddv62l10, 3' ...  1281   0.0    1
(BJ436781) Dictyostelium discoideum cDNA clone:ddv32p01, 3' ...  1277   0.0    1
dna update 2008.11.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P13676) RecName: Full=Acylamino-acid-releasing enzyme;         ...   161   5e-38
BC034199_1(BC034199|pid:none) Mus musculus acylpeptide hydrolase...   159   2e-37
BC026594_1(BC026594|pid:none) Mus musculus acylpeptide hydrolase...   159   2e-37
(Q8R146) RecName: Full=Acylamino-acid-releasing enzyme;         ...   159   2e-37
EU972122_1(EU972122|pid:none) Zea mays clone 375508 acylamino-ac...   157   7e-37
(P19205) RecName: Full=Acylamino-acid-releasing enzyme;         ...   157   7e-37
D38441_1(D38441|pid:none) Human mRNA for acylamino acid-releasin...   156   1e-36
AF100669_11(AF100669|pid:none) Caenorhabditis elegans cosmid R11...   156   2e-36
T33751(T33751)hypothetical protein R11E3.8 - Caenorhabditis eleg...   156   2e-36
(P80227) RecName: Full=Acylamino-acid-releasing enzyme;         ...   154   7e-36
protein update 2009. 7.13
PSORT 

 psg: 0.75  gvh: 0.26  alm: 0.39  top: 0.53  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.40  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 36.0 %: nuclear
	  8.0 %: vacuolar
	  4.0 %: mitochondrial
	  4.0 %: peroxisomal

>> prediction for VHI656 is cyt
5' end seq. ID VHI656F
5' end seq. 
>VHI656F.Seq
GAGACATAAAAGATGGAAAGTTTAAGTGAACAAGAAATAAAGTTTAGAGAAGATACAACT
AAAGTTTACAGGGAGTTATTATCAATTCCAACAGTCAACAACGCATTTTTTACAAACAAT
GATGAAAGTAATGAAAATCAAACATCAGCATCAATCATTGTATCTCAAATTGATATCACC
AATAAGAAAACTAAATTTTATATGTCACAAAAAACAATTTTAAAAGATTCATCATCATCA
TCAAAGAAAAACCAAGTAATTTCATCAGCAATTCAACAAGAATTAGCAACTTCAATCATA
TCAGTATCACCATCACATAAGAAAGTTTTAACAATTAAAGAGAACCCTTCAACTGAATTC
GAATTCTCTTTTGATATTACTGATGGTTCACATTTAATTACTTCAATTACTTCAAAAGAT
ATTCATAAAAAAATTTTAAATGATGAATGGTTTGGTAGTTTTTCATGGTCACCATGTGAG
AAATATGTAGCATTTATTGCAGATTCAAAATTAAATGTAACAGGATTTTTTGATAAGGAA
CCAAAAGATAAAGCTATTGGTGATCAATTTGTATTTAGAGANNNNNNNNNN
Length of 5' end seq. 591
3' end seq. ID VHI656Z
3' end seq. 
>VHI656Z.Seq
NNNNNNNNNNAATTTAGATGTTGGTTATGTTTCAACAATCACTTATTTACTCTCATTGGG
TTATAATATTATAATTCCAAATTATAGAGGTTCAACAGGTTATGGTAAAGATTTCGTAGA
TTGTTTACCAGGTTATATTGGTGATTTGGATGTTGACGATTGTGTTCAAGCAATTAATTA
CACACTCGATAAAATCGATAATACAATCGATAGAAATAAAATTGGTGTTATTGGTGGTAG
TCATGGTGGTTTCTTATCAGCACATCTAAGTAGATTCCCAATTGTCAAAACTTCAATCAT
GAGAAATCCTGTAATCGATATTCCATCAATGTCAACCTTATCCGATATTCCAGATTGGTG
TTTCTTTGAAGCTGGTATTAATCTCTCTGATCCAACCTCTCAATATCATACATTACCATC
AATGGAAGAAATTGAAAAAATGAGAAAATGTTCACCAAGTTTCCATATTGAAAACATCAA
AATTCCATCATTATTAGCTTTAGGTGATTCAGATCTTCGTGTTCCACCTTCTCAAGGTTT
ATTATATTATCGTATGTTAAGAGAAAGAGATGTACCAACAAAATGTTTAATGTATCCAAA
AACTGGTCATAGTCTTGATTCAATTGATGCTCGTTTAGATCAATGGGTTAATATTTCATT
ATGGTTAAAAAAATATTTAAATTAAAATTTTTAAAAAAAATTACAACAATTACACCACCA
CCACCACCACTACCACCACCATATGGTAAAATAATATTATTAAATAAAAACATTTTATCT
TNCTTTNTTATA
Length of 3' end seq. 792
Connected seq. ID VHI656P
Connected seq. 
>VHI656P.Seq
GAGACATAAAAGATGGAAAGTTTAAGTGAACAAGAAATAAAGTTTAGAGAAGATACAACT
AAAGTTTACAGGGAGTTATTATCAATTCCAACAGTCAACAACGCATTTTTTACAAACAAT
GATGAAAGTAATGAAAATCAAACATCAGCATCAATCATTGTATCTCAAATTGATATCACC
AATAAGAAAACTAAATTTTATATGTCACAAAAAACAATTTTAAAAGATTCATCATCATCA
TCAAAGAAAAACCAAGTAATTTCATCAGCAATTCAACAAGAATTAGCAACTTCAATCATA
TCAGTATCACCATCACATAAGAAAGTTTTAACAATTAAAGAGAACCCTTCAACTGAATTC
GAATTCTCTTTTGATATTACTGATGGTTCACATTTAATTACTTCAATTACTTCAAAAGAT
ATTCATAAAAAAATTTTAAATGATGAATGGTTTGGTAGTTTTTCATGGTCACCATGTGAG
AAATATGTAGCATTTATTGCAGATTCAAAATTAAATGTAACAGGATTTTTTGATAAGGAA
CCAAAAGATAAAGCTATTGGTGATCAATTTGTATTTAGAGA----------AATTTAGAT
GTTGGTTATGTTTCAACAATCACTTATTTACTCTCATTGGGTTATAATATTATAATTCCA
AATTATAGAGGTTCAACAGGTTATGGTAAAGATTTCGTAGATTGTTTACCAGGTTATATT
GGTGATTTGGATGTTGACGATTGTGTTCAAGCAATTAATTACACACTCGATAAAATCGAT
AATACAATCGATAGAAATAAAATTGGTGTTATTGGTGGTAGTCATGGTGGTTTCTTATCA
GCACATCTAAGTAGATTCCCAATTGTCAAAACTTCAATCATGAGAAATCCTGTAATCGAT
ATTCCATCAATGTCAACCTTATCCGATATTCCAGATTGGTGTTTCTTTGAAGCTGGTATT
AATCTCTCTGATCCAACCTCTCAATATCATACATTACCATCAATGGAAGAAATTGAAAAA
ATGAGAAAATGTTCACCAAGTTTCCATATTGAAAACATCAAAATTCCATCATTATTAGCT
TTAGGTGATTCAGATCTTCGTGTTCCACCTTCTCAAGGTTTATTATATTATCGTATGTTA
AGAGAAAGAGATGTACCAACAAAATGTTTAATGTATCCAAAAACTGGTCATAGTCTTGAT
TCAATTGATGCTCGTTTAGATCAATGGGTTAATATTTCATTATGGTTAAAAAAATATTTA
AATTAAAATTTTTAAAAAAAATTACAACAATTACACCACCACCACCACCACTACCACCAC
CATATGGTAAAATAATATTATTAAATAAAAACATTTTATCTTNCTTTNTTATA
Length of connected seq. 1363
Full length Seq ID -
Full length Seq. -
Length of full length seq. -