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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI649
Library VH
(Link to library)
Clone ID VHI649
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U13129-1
Original site URL
Representative seq. ID VHI649P
(Link to Original site)
Representative DNA sequence 
>VHI649 (VHI649Q) /CSM/VH/VHI6-C/VHI649Q.Seq.d/
AATATTATCTAAATATAGTAATAAAGGAATCTTTGGAGTAAAAGGAATGATCTCAGCAAT
GAGTGCAATTGGATTTTTAGGATTCTTAGTATGGGCTCATCACATGTACACTGTAGGATT
AGACGTGGATACACGAGCATATTTTACAGCAGCAACAATGATAATTGCAATCCCAACAGG
AATAAAAATTTTTAGTTGGTTAGCAACATTATGGGGAGGAGTAATAAAAATTACAACACC
AATGTTATTCGTAATAGGATTTTTAGTTCTATTTACAATAGGAGGATTAACAGGAGTAGT
ATTAGCAAATGGAGGATTAGATATTAGTTTACATGATACATATTATGTAGTAGCTCATTT
TCATTATGTATTATCAATGGGAGCAATATTTGCAATATTTGCAGGATATTATTATTATTA
TTCAATTATGAATAGTACAAGATTATTCGGAGTAGTCCGATATAATGAACAATTAGGAAG
AATACATTTCTGGACAATGTTTATTGGAGTAAATGTTACATTTTTTCCAATGCATTTCTT
AGGATTGGCAGGTATGCCACGTAGAATXXXXXXXXXXACAGCTATTGCAAAAATGGTTAT
AAGTATTTGTGTTATCTTTGCAGGTTATTATACATTATACTCTGCCTACGCAGAGTATAA
CCAAATTGTAGAACAAAATGCACAAGCTGTTCTAAGCGAAGTAACAAGTAGTAATATGCC
CGTACCGCCATTAGAGAGTTTAACTGATATTTTAGTAGCATCTGAAAAATTATCTCCGCA
AAGTAAAGTAAATTTTTTATTAGAGCATCAAACAAAGATAATCTCCTTACTAAAGGTTCA
GGGGGATACCTTATCTCCCGATCAAAAAGAGAATTTGAAGGAAATTGTAAATTTTTTAAC
AAAAAGAATAGAAGATACTAGGGAACAAGCTAATAAAATAATAAAATAGCAATGAAGGAC
TTTATTAAAGAATGTAACAAATACATTGACAATAAAAAAAAATAGTATAGATCAATAATA
AACTAATAGAAAAGATAAGGTATTAAGGGTATTCTACTCTTTTAATCTTTTAATATCTAA
TAGGATAGCAAGAATAGTAAAGAGATGAGGTGTAAAATATAAAAGTAAATGTACACCCTT
AATTAACGAAAAAGCTTTTAAATGGGAATTGTCAAACTAAAAACCTCGCCGTTCAGAGAA
AGAGAATGGTTGTTAACATT
sequence update 2002.10.25
Translated Amino Acid sequence 
ILSKYSNKGIFGVKGMISAMSAIGFLGFLVWAHHMYTVGLDVDTRAYFTAATMIIAIPTG
IKIFSWLATLWGGVIKITTPMLFVIGFLVLFTIGGLTGVVLANGGLDISLHDTYYVVAHF
HYVLSMGAIFAIFAGYYYYYSIMNSTRLFGVVRYNEQLGRIHFWTMFIGVNVTFFPMHFL
GLAGMPRR---

---TAIAKMVISICVIFAGYYTLYSAYAEYNQIVEQNAQAVLSEVTSSNMPVPPLESLTD
ILVASEKLSPQSKVNFLLEHQTKIISLLKVQGDTLSPDQKENLKEIVNFLTKRIEDTREQ
ANKIIK*q*rtllknvtntltikknsidq**tnrkdkvlrvfysfnllisnriarivkr*
gvkykskctplinekafkwelsn*kprrsekengc*h
Translated Amino Acid sequence (All Frames) 
Frame A:
nii*i***rnlwskrndlsnecnwifrilsmgsshvhcrirrgytsifyssnndncnpnr
nknf*lvsnimgrsnknyntnvirnrifssiynrrinrssiskwriry*ft*yilcsssf
slciingsnicnicrillllfnye*ykiirsspi**tirkntfldnvywskcyifsnafl
rigryat*n---

---TAIAKMVISICVIFAGYYTLYSAYAEYNQIVEQNAQAVLSEVTSSNMPVPPLESLTD
ILVASEKLSPQSKVNFLLEHQTKIISLLKVQGDTLSPDQKENLKEIVNFLTKRIEDTREQ
ANKIIK*q*rtllknvtntltikknsidq**tnrkdkvlrvfysfnllisnriarivkr*
gvkykskctplinekafkwelsn*kprrsekengc*h

Frame B:
ILSKYSNKGIFGVKGMISAMSAIGFLGFLVWAHHMYTVGLDVDTRAYFTAATMIIAIPTG
IKIFSWLATLWGGVIKITTPMLFVIGFLVLFTIGGLTGVVLANGGLDISLHDTYYVVAHF
HYVLSMGAIFAIFAGYYYYYSIMNSTRLFGVVRYNEQLGRIHFWTMFIGVNVTFFPMHFL
GLAGMPRR---

---qllqkwl*vfvlslqviihytlptqsitkl*nkmhklf*ak*qvvicpyrh*rv*li
f**hlknylrkvk*ify*sikqr*spy*rfrgipylpikkri*rkl*if*qke*kilgnk
lik**nsnegly*rm*qih*q*kkiv*innkliekiry*gystllif*ylig*qe**rde
v*nikvnvhp*ltkkllngncqtknlavqrkrmvvni

Frame C:
yylnivikesle*ke*sqq*vqldf*ds*yglitctl*d*twihehilqqqq**lqsqqe
*kflvg*qhygee**klqhqcys**df*fylq*ed*qe*y*qmed*ilvymihim**lif
imyyqweqylqylqdiiiiiql*ivqdyse*sdimnn*eeyisgqclle*mlhffqcis*
dwqvchve---

---syckngykylcylcrllyiilclrrv*pncrtkctscskrsnk**yartairefn*y
fssi*kiisak*skffirasnkdnlltkgsggylisrskrefegnckffnkknrry*gts
**nnkiamkdfikecnkyidnkkk*yrsiin**kr*gikgillf*sfni**dsknskemr
cki*k*mytln*rksf*mgivklktspfrerewllt
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI649 (VHI649Q) /CSM/VH/VHI6-C/VHI649Q.Seq.d/                       2305   0.0
VHB602 (VHB602Q) /CSM/VH/VHB6-A/VHB602Q.Seq.d/                       1221   0.0
VHN576 (VHN576Q) /CSM/VH/VHN5-D/VHN576Q.Seq.d/                       1207   0.0
VHJ852 (VHJ852Q) /CSM/VH/VHJ8-C/VHJ852Q.Seq.d/                       1205   0.0
VHP858 (VHP858Q) /CSM/VH/VHP8-C/VHP858Q.Seq.d/                       1181   0.0
MIT-GENOME (MIT-GENOMEQ) /CSM/OTHERS/MIT-GENOMEQ.Seq.d/              1158   0.0
VSE881 (VSE881Q) /CSM/VS/VSE8-D/VSE881Q.Seq.d/                       1150   0.0
SHH491 (SHH491Q) /CSM/SH/SHH4-D/SHH491Q.Seq.d/                       1128   0.0
VHB379 (VHB379Q) /CSM/VH/VHB3-D/VHB379Q.Seq.d/                       1065   0.0
VSK250 (VSK250Q) /CSM/VS/VSK2-C/VSK250Q.Seq.d/                        615   e-175
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ445221) Dictyostelium discoideum cDNA clone:ddv58g19, 3' ...   825   0.0    3
(BJ441986) Dictyostelium discoideum cDNA clone:ddv48a14, 3' ...   825   0.0    2
(BJ437357) Dictyostelium discoideum cDNA clone:ddv34c02, 3' ...   825   0.0    2
(BJ442660) Dictyostelium discoideum cDNA clone:ddv50h14, 3' ...   823   0.0    3
(BJ446379) Dictyostelium discoideum cDNA clone:ddv62d16, 3' ...   809   0.0    2
(AU263453) Dictyostelium discoideum vegetative cDNA clone:VS...   753   0.0    2
(BJ407519) Dictyostelium discoideum cDNA clone:dds38f24, 3' ...   739   0.0    2
(AB000109) Dictyostelium discoideum mitochondrial DNA, compl...   670   0.0    6
(BJ423223) Dictyostelium discoideum cDNA clone:ddv48a14, 5' ...   729   0.0    2
(X81884) D.discoideum mitochondrial cox1 gene and cox2 gene.      670   0.0    3
dna update 2008.11.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

X81884_2(X81884|pid:none) D.discoideum mitochondrial cox1 gene a...   349   1e-94
(O21042) RecName: Full=Cytochrome c oxidase subunit 1+2;        ...   349   1e-94
DQ336395_6(DQ336395|pid:none) Dictyostelium citrinum mitochondri...   341   4e-92
EU275726_6(EU275726|pid:none) Polysphondylium pallidum strain PN...   320   5e-86
EU275727_7(EU275727|pid:none) Dictyostelium fasciculatum strain ...   317   5e-85
AY700145_35(AY700145|pid:none) Polysphondylium pallidum mitochon...   316   1e-84
EU069530_1(EU069530|pid:none) Plantago spathulata cytochrome oxi...   266   1e-69
EU069526_1(EU069526|pid:none) Plantago raoulii cytochrome oxidas...   266   1e-69
EU069521_1(EU069521|pid:none) Plantago major cytochrome oxidase ...   266   1e-69
AY494079_25(AY494079|pid:none) Fucus vesiculosus mitochondrion, ...   265   2e-69
protein update 2009. 7.13
PSORT 

 psg: 0.83  gvh: 0.46  alm: 0.23  top: 0.27  tms: 0.33  mit: 0.27  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 1.00  m3b: 0.00  m_ : 0.00 

	 40.0 %: endoplasmic reticulum
	 20.0 %: mitochondrial
	 16.0 %: plasma membrane
	  8.0 %: extracellular, including cell wall
	  4.0 %: Golgi
	  4.0 %: nuclear
	  4.0 %: vesicles of secretory system
	  4.0 %: cytoplasmic

>> prediction for VHI649 is end
5' end seq. ID VHI649F
5' end seq. 
>VHI649F.Seq
AATATTATCTAAATATAGTAATAAAGGAATCTTTGGAGTAAAAGGAATGATCTCAGCAAT
GAGTGCAATTGGATTTTTAGGATTCTTAGTATGGGCTCATCACATGTACACTGTAGGATT
AGACGTGGATACACGAGCATATTTTACAGCAGCAACAATGATAATTGCAATCCCAACAGG
AATAAAAATTTTTAGTTGGTTAGCAACATTATGGGGAGGAGTAATAAAAATTACAACACC
AATGTTATTCGTAATAGGATTTTTAGTTCTATTTACAATAGGAGGATTAACAGGAGTAGT
ATTAGCAAATGGAGGATTAGATATTAGTTTACATGATACATATTATGTAGTAGCTCATTT
TCATTATGTATTATCAATGGGAGCAATATTTGCAATATTTGCAGGATATTATTATTATTA
TTCAATTATGAATAGTACAAGATTATTCGGAGTAGTCCGATATAATGAACAATTAGGAAG
AATACATTTCTGGACAATGTTTATTGGAGTAAATGTTACATTTTTTCCAATGCATTTCTT
AGGATTGGCAGGTATGCCACGTAGAATNNNNNNNNNN
Length of 5' end seq. 577
3' end seq. ID VHI649Z
3' end seq. 
>VHI649Z.Seq
NNNNNNNNNNACAGCTATTGCAAAAATGGTTATAAGTATTTGTGTTATCTTTGCAGGTTA
TTATACATTATACTCTGCCTACGCAGAGTATAACCAAATTGTAGAACAAAATGCACAAGC
TGTTCTAAGCGAAGTAACAAGTAGTAATATGCCCGTACCGCCATTAGAGAGTTTAACTGA
TATTTTAGTAGCATCTGAAAAATTATCTCCGCAAAGTAAAGTAAATTTTTTATTAGAGCA
TCAAACAAAGATAATCTCCTTACTAAAGGTTCAGGGGGATACCTTATCTCCCGATCAAAA
AGAGAATTTGAAGGAAATTGTAAATTTTTTAACAAAAAGAATAGAAGATACTAGGGAACA
AGCTAATAAAATAATAAAATAGCAATGAAGGACTTTATTAAAGAATGTAACAAATACATT
GACAATAAAAAAAAATAGTATAGATCAATAATAAACTAATAGAAAAGATAAGGTATTAAG
GGTATTCTACTCTTTTAATCTTTTAATATCTAATAGGATAGCAAGAATAGTAAAGAGATG
AGGTGTAAAATATAAAAGTAAATGTACACCCTTAATTAACGAAAAAGCTTTTAAATGGGA
ATTGTCAAACTAAAAACCTCGCCGTTCAGAGAAAGAGAATGGTTGTTAACATT
Length of 3' end seq. 653
Connected seq. ID VHI649P
Connected seq. 
>VHI649P.Seq
AATATTATCTAAATATAGTAATAAAGGAATCTTTGGAGTAAAAGGAATGATCTCAGCAAT
GAGTGCAATTGGATTTTTAGGATTCTTAGTATGGGCTCATCACATGTACACTGTAGGATT
AGACGTGGATACACGAGCATATTTTACAGCAGCAACAATGATAATTGCAATCCCAACAGG
AATAAAAATTTTTAGTTGGTTAGCAACATTATGGGGAGGAGTAATAAAAATTACAACACC
AATGTTATTCGTAATAGGATTTTTAGTTCTATTTACAATAGGAGGATTAACAGGAGTAGT
ATTAGCAAATGGAGGATTAGATATTAGTTTACATGATACATATTATGTAGTAGCTCATTT
TCATTATGTATTATCAATGGGAGCAATATTTGCAATATTTGCAGGATATTATTATTATTA
TTCAATTATGAATAGTACAAGATTATTCGGAGTAGTCCGATATAATGAACAATTAGGAAG
AATACATTTCTGGACAATGTTTATTGGAGTAAATGTTACATTTTTTCCAATGCATTTCTT
AGGATTGGCAGGTATGCCACGTAGAAT----------ACAGCTATTGCAAAAATGGTTAT
AAGTATTTGTGTTATCTTTGCAGGTTATTATACATTATACTCTGCCTACGCAGAGTATAA
CCAAATTGTAGAACAAAATGCACAAGCTGTTCTAAGCGAAGTAACAAGTAGTAATATGCC
CGTACCGCCATTAGAGAGTTTAACTGATATTTTAGTAGCATCTGAAAAATTATCTCCGCA
AAGTAAAGTAAATTTTTTATTAGAGCATCAAACAAAGATAATCTCCTTACTAAAGGTTCA
GGGGGATACCTTATCTCCCGATCAAAAAGAGAATTTGAAGGAAATTGTAAATTTTTTAAC
AAAAAGAATAGAAGATACTAGGGAACAAGCTAATAAAATAATAAAATAGCAATGAAGGAC
TTTATTAAAGAATGTAACAAATACATTGACAATAAAAAAAAATAGTATAGATCAATAATA
AACTAATAGAAAAGATAAGGTATTAAGGGTATTCTACTCTTTTAATCTTTTAATATCTAA
TAGGATAGCAAGAATAGTAAAGAGATGAGGTGTAAAATATAAAAGTAAATGTACACCCTT
AATTAACGAAAAAGCTTTTAAATGGGAATTGTCAAACTAAAAACCTCGCCGTTCAGAGAA
AGAGAATGGTTGTTAACATT
Length of connected seq. 1210
Full length Seq ID -
Full length Seq. -
Length of full length seq. -