>VHI643 (VHI643Q) /CSM/VH/VHI6-B/VHI643Q.Seq.d/
XXXXXXXXXXACACTCAACAAGCTGGTGGTGATGNTGAAGATGATCAATGTGATACCATT
TTATCACAACTTCCACAACCAAGTGAAATGGCTGGTTACAAAATTANCTCTATTCAATTT
GAAAAGGATGATGATACCAATCATCATATCGATTTCATTACCGCTACNTCAAATTTAAGA
GCTACCAACTATGCAATCAGTCCAGCTGATAAACATAAGACCAAAGGTATCGCCGGTAAA
ATCATTCCAGCTTTAGTTACCACTACTGCCGTAGNTGCTGGTTTCGTTTGTATCGAATTG
ATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATAAGAGCACCTTTATGAATTTAGGT
ATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCCCAAAGAACAAAATTCGTGAAGGC
TGGACTTGGACACTTTGGGATCGTTTCGATGTTGATGGTGATATCACTCTCAAGGAGTTT
TTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTTCAATGCTCTCTTGTAAAGTTACA
CTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAGAGGAAAGATTAAAGATGAAAATC
TCACAACTCTATGAAACCCTCTCAAAGAAACCATTACCAAAAGATAAGAAATATTTATTA
CTGAAATTTGTTGCAA

---TQQAGGDXEDDQCDTILSQLPQPSEMAGYKIXSIQFEKDDDTNHHIDFITATSNLRA
TNYAISPADKHKTKGIAGKIIPALVTTTAVXAGFVCIELIKVIQNKALEKYKSTFMNLGI
PFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLFEKKHGLDISMLSCKVTL
LYALFTDKKTKEERLKMKISQLYETLSKKPLPKDKKYLLLKFVA

Frame A:
---hstsww*x*r*sm*yhfittsttk*ngwlqnxlysi*kg**yqssyrfhyryxkfks
yqlcnqss**t*dqryrr*nhssfsyhycrxcwfrlyrid*sysk*sfgei*ehlyefry
pilwfr*tncrpkeqns*rldldtlgsfrc*w*yhsqgvfrsl*ketwfgyfnall*syt
ficslh**en*rgkikdenlttl*nplketitkr*eifiteicc


Frame B:
---TQQAGGDXEDDQCDTILSQLPQPSEMAGYKIXSIQFEKDDDTNHHIDFITATSNLRA
TNYAISPADKHKTKGIAGKIIPALVTTTAVXAGFVCIELIKVIQNKALEKYKSTFMNLGI
PFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLFEKKHGLDISMLSCKVTL
LYALFTDKKTKEERLKMKISQLYETLSKKPLPKDKKYLLLKFVA


Frame C:
---lnklvvmxkminvipfyhnfhnqvkwlvtklxlfnlkrmmipiiisislplxqi*el
ptmqsvqlinirpkvspvksfql*lpllp*xlvsfvsn*lklfkiklwrnirapl*i*vs
hslvslnqlppqrtkfvkaglghfgivsmlmvislsrsf*islkrnmvwifqcslvklhf
ymlsslirklkrkd*r*kshnsmkpsqrnhyqkirniyy*nllq

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHP750 (VHP750Q) /CSM/VH/VHP7-C/VHP750Q.Seq.d/                       1296   0.0
VHP205 (VHP205Q) /CSM/VH/VHP2-A/VHP205Q.Seq.d/                       1296   0.0
VHN723 (VHN723Q) /CSM/VH/VHN7-A/VHN723Q.Seq.d/                       1296   0.0
VHI643 (VHI643Q) /CSM/VH/VHI6-B/VHI643Q.Seq.d/                       1296   0.0
VHB744 (VHB744Q) /CSM/VH/VHB7-B/VHB744Q.Seq.d/                       1296   0.0
VHA328 (VHA328Q) /CSM/VH/VHA3-B/VHA328Q.Seq.d/                       1296   0.0
SHK573 (SHK573Q) /CSM/SH/SHK5-D/SHK573Q.Seq.d/                       1296   0.0
SHD849 (SHD849Q) /CSM/SH/SHD8-C/SHD849Q.Seq.d/                       1296   0.0
SHC572 (SHC572Q) /CSM/SH/SHC5-C/SHC572Q.Seq.d/                       1296   0.0
SHC430 (SHC430Q) /CSM/SH/SHC4-B/SHC430Q.Seq.d/                       1296   0.0

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

AZ930776|AZ930776.1 474.dhz58e03.s1 Saccharomyces unisporus NRRL Y-1556 Saccharomycesunisporus genomic clone 474.dhz58e03.s1, genomic survey sequence.    68   2e-12  2
CB933531|CB933531.1 ri31b11.y1 Meloidogyne chitwoodi egg pAMP1 v1 Meloidogyne chitwoodi cDNA 5' similar to TR:Q27481 Q27481 C47E12.5 PROTEIN. [1] ;, mRNA sequence.    58   2e-04  1
BZ303839|BZ303839.1 KD2618.q1 Kluyveromyces delphensis Random Genomic Library Kluyveromyces delphensis genomic clone KD2618, DNA sequence.    48   3e-04  2
BH154952|BH154952.1 ENTTP11TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   0.002  2
BH163825|BH163825.1 ENTSW62TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   0.002  2
BH151170|BH151170.1 ENTPM35TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   0.002  2
BH158699|BH158699.1 ENTQX37TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   0.002  2
AZ686606|AZ686606.1 ENTIE44TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    52   0.002  2
BG799138|BG799138.1 fp18f06.y1 zebrafish gridded kidney Danio rerio cDNA clone 4729883 5' similar to SW:UBAX_MOUSE P31253 UBIQUITIN-ACTIVATING ENZYME E1 X ;, mRNA sequence.    34   0.005  3
AL403306|AL403306.1 T3 end of clone AT0AA007G10 of library AT0AA from strain CBS 4311 of Saccharomyces servazzii.    44   0.009  2

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55C16) RecName: Full=Ubiquitin-like modifier-activating enzyme...   403   e-111
EF468345_1(EF468345|pid:none) Pisum sativum ubiquitin activating...   230   3e-59
AE017341_129(AE017341|pid:none) Cryptococcus neoformans var. neo...   219   8e-56
CR382138_755(CR382138|pid:none) Debaryomyces hansenii strain CBS...   216   7e-55
FN392321_874(FN392321|pid:none) Pichia pastoris GS115 chromosome...   210   4e-53
CP000496_820(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   209   5e-53
AB035495_1(AB035495|pid:none) Carassius auratus mRNA for ubiquit...   208   1e-52
AE016819_666(AE016819|pid:none) Ashbya gossypii (= Eremothecium ...   207   2e-52
(P22515) RecName: Full=Ubiquitin-activating enzyme E1 1;  &S3804...   207   2e-52
BC047256_1(BC047256|pid:none) Xenopus laevis ubiquitin-like modi...   205   9e-52

 psg: 0.75  gvh: 0.42  alm: 0.31  top: 0.43  tms: 0.07  mit: 0.19  mip: 0.00
 nuc: 0.09  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: cytoplasmic
	 24.0 %: nuclear
	 16.0 %: endoplasmic reticulum
	  8.0 %: cytoskeletal
	  8.0 %: mitochondrial
	  4.0 %: peroxisomal
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHI643 is cyt

>VHI643Z.Seq
NNNNNNNNNNACACTCAACAAGCTGGTGGTGATGNTGAAGATGATCAATGTGATACCATT
TTATCACAACTTCCACAACCAAGTGAAATGGCTGGTTACAAAATTANCTCTATTCAATTT
GAAAAGGATGATGATACCAATCATCATATCGATTTCATTACCGCTACNTCAAATTTAAGA
GCTACCAACTATGCAATCAGTCCAGCTGATAAACATAAGACCAAAGGTATCGCCGGTAAA
ATCATTCCAGCTTTAGTTACCACTACTGCCGTAGNTGCTGGTTTCGTTTGTATCGAATTG
ATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATAAGAGCACCTTTATGAATTTAGGT
ATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCCCAAAGAACAAAATTCGTGAAGGC
TGGACTTGGACACTTTGGGATCGTTTCGATGTTGATGGTGATATCACTCTCAAGGAGTTT
TTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTTCAATGCTCTCTTGTAAAGTTACA
CTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAGAGGAAAGATTAAAGATGAAAATC
TCACAACTCTATGAAACCCTCTCAAAGAAACCATTACCAAAAGATAAGAAATATTTATTA
CTGAAATTTGTTGCAA