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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI617
Library VH
(Link to library)
Clone ID VHI617
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16251-1
Original site URL
Representative seq. ID VHI617P
(Link to Original site)
Representative DNA sequence 
>VHI617 (VHI617Q) /CSM/VH/VHI6-A/VHI617Q.Seq.d/
TGCAAGCCATTCTTTAAGTTCACTAGCTATTACTCATCAAGATAAAATTGTATTAAAACC
TGAAAATATTTCATTCAATGAAGCTGCTGCCGTTTGTGTAGTTTATGCAACAAGTTATTA
TTCAATTTTCCATATTGGTGCATTTATGGCCGATAAAGAATCAATCCTAGTTCATTCTGC
AACTGGTGGTGTTGGTTTAGCAACTTTAAATCTTTTAAAATGGAAGACAAATCAATTGAA
AAAACATGGCAACTCTGAAATTAGTAATGATGCTTCAATTTATGCTACAGTGGGTTCTAA
AGAAAAAGTCGATTATTTACAAGAGAAATATGGTGATTTAATAACTGCAATTTACAATAG
TAGAGATACTGAATATTGCGATGAAATTAAACAACAATCAGCACAAGGAGGTGTAGATTT
AATTTTAAATACACTCTCTGGTGATTATTTATCTGCAAATTTCCGTTCACTTTCCCAAGT
TGGTAGAATTATGGATTTATCAGTCACTCAATTGGTTGAAAATGATTCATTAGATTTTTC
AAACTTTAAGTATCATGTTACTTACAGTACAATCGATTTAGAAAGAGCTACAACATATAA
TTCAAAAATCGTXXXXXXXXXXGAAATTAGAAAACTTTATTCTCTTTTCATCAATTGGTG
CTATCATTGGTGGTTCAAAACAATGTGCATATAGTAGTGCTAATTTAGTATTGGATTCAT
TATCAAACTATAGAAAGAGTATTGGTCTTGCTTCAACTTCAATTAATTGGGGTGGTTTGG
ATGCTGGTGGTGTTGCCGCCACTGATAAATCAGTCGCAAGTTTCTTAGAGGGTCAAGGTA
TTTTATTGGTTTCTTTATCAAAGATTTTAGGTTGTCTTGATTCAGTTTTCCAACCATCTA
ATTCCCATTTATCAAACTTTATGCTTTCAAGTTTCAATATTGATAATCTTTTATCTTCTG
CACCTCAAATGAAGAGAAAAATGGGTCACCACCTAACAAATTATAAAACTTCATCTGCTA
GCTCTGATGATTCATTGGGTGATTCTAGTTCTACTCAAGCAAAAGTCATTTCTACAATCT
CTGAATTATTATCAATTCATCCATCAAAATTAAATTTAGATACAAGATTAAAAGACTATG
GTATTGATTCTTTATTAACAGTACAATTAAAGAATTGGATAGATAAAGAATTTACAAAGA
ATTTATTCACTCATTTACAATTATCATCAAGTAGTATTAATAGTATCATTCAGAGAATTA
GTTCAAAATCAACTTCAACATCTACTCCCAATCCAACTAACACCACAAAACAAACCGCAA
CAACAAAAACATAAGTAGTTCAAACAACTCCAACTACATT
sequence update 2002.10.25
Translated Amino Acid sequence 
ASHSLSSLAITHQDKIVLKPENISFNEAAAVCVVYATSYYSIFHIGAFMADKESILVHSA
TGGVGLATLNLLKWKTNQLKKHGNSEISNDASIYATVGSKEKVDYLQEKYGDLITAIYNS
RDTEYCDEIKQQSAQGGVDLILNTLSGDYLSANFRSLSQVGRIMDLSVTQLVENDSLDFS
NFKYHVTYSTIDLERATTYNSKI---

---KLENFILFSSIGAIIGGSKQCAYSSANLVLDSLSNYRKSIGLASTSINWGGLDAGGV
AATDKSVASFLEGQGILLVSLSKILGCLDSVFQPSNSHLSNFMLSSFNIDNLLSSAPQMK
RKMGHHLTNYKTSSASSDDSLGDSSSTQAKVISTISELLSIHPSKLNLDTRLKDYGIDSL
LTVQLKNWIDKEFTKNLFTHLQLSSSSINSIIQRISSKSTSTSTPNPTNTTKQTATTKT*
vvqttptt
Translated Amino Acid sequence (All Frames) 
Frame A:
ckpffkftsyyssr*ncikt*kyfiq*sccrlcslcnkllfnfpywciygr*rinpssfc
nwwcwfsnfksfkmedksiektwql*n***cfnlcysgf*rksrlftreiw*fnncnlq*
*ry*ilr*n*ttistrrcrfnfkytlw*lfickfpftfpsw*nygfishsig*k*firff
kl*vscylqynrfrksyni*fknr---

---eirklyslfinwcyhwwfktmci**c*fsigfiikl*keywscfnfn*lgwfgcwwc
crh**isrkflrgsryfigffikdfrls*fsfpti*fpfiklyafkfqy**sfifctsne
ekngsppnkl*nfic*l**fig*f*fysskshfynl*iiinssikikfrykikrlwy*ff
instikeldr*riykefihsftiiik*y**yhsen*fkinfniysqsn*hhktnrnnkni
sssnnsnyi

Frame B:
ASHSLSSLAITHQDKIVLKPENISFNEAAAVCVVYATSYYSIFHIGAFMADKESILVHSA
TGGVGLATLNLLKWKTNQLKKHGNSEISNDASIYATVGSKEKVDYLQEKYGDLITAIYNS
RDTEYCDEIKQQSAQGGVDLILNTLSGDYLSANFRSLSQVGRIMDLSVTQLVENDSLDFS
NFKYHVTYSTIDLERATTYNSKI---

---KLENFILFSSIGAIIGGSKQCAYSSANLVLDSLSNYRKSIGLASTSINWGGLDAGGV
AATDKSVASFLEGQGILLVSLSKILGCLDSVFQPSNSHLSNFMLSSFNIDNLLSSAPQMK
RKMGHHLTNYKTSSASSDDSLGDSSSTQAKVISTISELLSIHPSKLNLDTRLKDYGIDSL
LTVQLKNWIDKEFTKNLFTHLQLSSSSINSIIQRISSKSTSTSTPNPTNTTKQTATTKT*
vvqttptt

Frame C:
qail*vh*lllikikly*nlkifhsmkllpfv*fmqqviiqfsilvhlwpiknqs*filq
lvvlv*ql*if*ngrqin*knmatlklvmmlqfmlqwvlkkksiiykrnmvi**lqftiv
eilniamklnnnqhkev*i*f*ihslviiylqisvhfpklvelwiyqslnwlkmih*ifq
tlsimlltvqsi*kelqhiiqks---

---n*ktlfsfhqlvlslvvqnnvhivvli*ywihyqtiervlvllqlqligvvwmlvvl
pplinqsqvs*rvkvfywflyqrf*vvliqfsnhlipiyqtlcfqvsiliifyllhlk*r
ekwvtt*qiiklhllalmihwvilvllkqksflqslnyyqfihqn*i*iqd*ktmvlily
*qyn*rig*iknlqriysliynyhqvvlivsfrelvqnqlqhllpiqltpqnkpqqqkhk
*fkqlqlh
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI617 (VHI617Q) /CSM/VH/VHI6-A/VHI617Q.Seq.d/                       2637   0.0
VHO568 (VHO568Q) /CSM/VH/VHO5-C/VHO568Q.Seq.d/                       1463   0.0
VHI485 (VHI485Q) /CSM/VH/VHI4-D/VHI485Q.Seq.d/                       1463   0.0
VHH639 (VHH639Q) /CSM/VH/VHH6-B/VHH639Q.Seq.d/                       1463   0.0
VHD848 (VHD848Q) /CSM/VH/VHD8-B/VHD848Q.Seq.d/                       1463   0.0
VHB382 (VHB382Q) /CSM/VH/VHB3-D/VHB382Q.Seq.d/                       1463   0.0
VHB174 (VHB174Q) /CSM/VH/VHB1-D/VHB174Q.Seq.d/                       1463   0.0
VHB109 (VHB109Q) /CSM/VH/VHB1-A/VHB109Q.Seq.d/                       1463   0.0
VHA188 (VHA188Q) /CSM/VH/VHA1-D/VHA188Q.Seq.d/                       1463   0.0
VHK683 (VHK683Q) /CSM/VH/VHK6-D/VHK683Q.Seq.d/                       1461   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(AC116305) Dictyostelium discoideum chromosome 2 map 1005175...  1281   0.0    1
(BJ445768) Dictyostelium discoideum cDNA clone:ddv60g17, 3' ...  1281   0.0    1
(BJ444858) Dictyostelium discoideum cDNA clone:ddv57l17, 3' ...  1281   0.0    1
(BJ443397) Dictyostelium discoideum cDNA clone:ddv52e22, 3' ...  1281   0.0    1
(BJ441827) Dictyostelium discoideum cDNA clone:ddv48a06, 3' ...  1281   0.0    1
(BJ441787) Dictyostelium discoideum cDNA clone:ddv47j22, 3' ...  1281   0.0    1
(BJ441341) Dictyostelium discoideum cDNA clone:ddv46m10, 3' ...  1281   0.0    1
(BJ438786) Dictyostelium discoideum cDNA clone:ddv38p12, 3' ...  1281   0.0    1
(BJ438461) Dictyostelium discoideum cDNA clone:ddv37c01, 3' ...  1281   0.0    1
(BJ437384) Dictyostelium discoideum cDNA clone:ddv34h05, 3' ...  1281   0.0    1
dna update 2008.11.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q869W9) RecName: Full=Probable polyketide synthase 16;         ...   399   e-109
(Q869X2) RecName: Full=Probable polyketide synthase 17;         ...   379   e-103
AC117075_22(AC117075|pid:none) Dictyostelium discoideum chromoso...   176   2e-42
(Q54B49) RecName: Full=Probable polyketide synthase 45;         ...   166   2e-39
(Q54TW0) RecName: Full=Probable polyketide synthase 18;         ...   164   9e-39
(Q54ED6) RecName: Full=Probable polyketide synthase 41;         ...   162   3e-38
AC116963_19(AC116963|pid:none) Dictyostelium discoideum chromoso...   160   1e-37
(Q55DM7) RecName: Full=Probable polyketide synthase 2;          ...   157   8e-37
AC116963_16(AC116963|pid:none) Dictyostelium discoideum chromoso...   150   8e-35
(Q54FN7) RecName: Full=Probable polyketide synthase 33;         ...   150   1e-34
protein update 2009. 7.13
PSORT 

 psg: 0.89  gvh: 0.42  alm: 0.31  top: 0.50  tms: 0.07  mit: 0.22  mip: 0.00
 nuc: 0.04  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.02  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 16.0 %: mitochondrial
	 16.0 %: nuclear
	  8.0 %: Golgi
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHI617 is end
5' end seq. ID VHI617F
5' end seq. 
>VHI617F.Seq
TGCAAGCCATTCTTTAAGTTCACTAGCTATTACTCATCAAGATAAAATTGTATTAAAACC
TGAAAATATTTCATTCAATGAAGCTGCTGCCGTTTGTGTAGTTTATGCAACAAGTTATTA
TTCAATTTTCCATATTGGTGCATTTATGGCCGATAAAGAATCAATCCTAGTTCATTCTGC
AACTGGTGGTGTTGGTTTAGCAACTTTAAATCTTTTAAAATGGAAGACAAATCAATTGAA
AAAACATGGCAACTCTGAAATTAGTAATGATGCTTCAATTTATGCTACAGTGGGTTCTAA
AGAAAAAGTCGATTATTTACAAGAGAAATATGGTGATTTAATAACTGCAATTTACAATAG
TAGAGATACTGAATATTGCGATGAAATTAAACAACAATCAGCACAAGGAGGTGTAGATTT
AATTTTAAATACACTCTCTGGTGATTATTTATCTGCAAATTTCCGTTCACTTTCCCAAGT
TGGTAGAATTATGGATTTATCAGTCACTCAATTGGTTGAAAATGATTCATTAGATTTTTC
AAACTTTAAGTATCATGTTACTTACAGTACAATCGATTTAGAAAGAGCTACAACATATAA
TTCAAAAATCGTNNNNNNNNNN
Length of 5' end seq. 622
3' end seq. ID VHI617Z
3' end seq. 
>VHI617Z.Seq
NNNNNNNNNNGAAATTAGAAAACTTTATTCTCTTTTCATCAATTGGTGCTATCATTGGTG
GTTCAAAACAATGTGCATATAGTAGTGCTAATTTAGTATTGGATTCATTATCAAACTATA
GAAAGAGTATTGGTCTTGCTTCAACTTCAATTAATTGGGGTGGTTTGGATGCTGGTGGTG
TTGCCGCCACTGATAAATCAGTCGCAAGTTTCTTAGAGGGTCAAGGTATTTTATTGGTTT
CTTTATCAAAGATTTTAGGTTGTCTTGATTCAGTTTTCCAACCATCTAATTCCCATTTAT
CAAACTTTATGCTTTCAAGTTTCAATATTGATAATCTTTTATCTTCTGCACCTCAAATGA
AGAGAAAAATGGGTCACCACCTAACAAATTATAAAACTTCATCTGCTAGCTCTGATGATT
CATTGGGTGATTCTAGTTCTACTCAAGCAAAAGTCATTTCTACAATCTCTGAATTATTAT
CAATTCATCCATCAAAATTAAATTTAGATACAAGATTAAAAGACTATGGTATTGATTCTT
TATTAACAGTACAATTAAAGAATTGGATAGATAAAGAATTTACAAAGAATTTATTCACTC
ATTTACAATTATCATCAAGTAGTATTAATAGTATCATTCAGAGAATTAGTTCAAAATCAA
CTTCAACATCTACTCCCAATCCAACTAACACCACAAAACAAACCGCAACAACAAAAACAT
AAGTAGTTCAAACAACTCCAACTACATT
Length of 3' end seq. 748
Connected seq. ID VHI617P
Connected seq. 
>VHI617P.Seq
TGCAAGCCATTCTTTAAGTTCACTAGCTATTACTCATCAAGATAAAATTGTATTAAAACC
TGAAAATATTTCATTCAATGAAGCTGCTGCCGTTTGTGTAGTTTATGCAACAAGTTATTA
TTCAATTTTCCATATTGGTGCATTTATGGCCGATAAAGAATCAATCCTAGTTCATTCTGC
AACTGGTGGTGTTGGTTTAGCAACTTTAAATCTTTTAAAATGGAAGACAAATCAATTGAA
AAAACATGGCAACTCTGAAATTAGTAATGATGCTTCAATTTATGCTACAGTGGGTTCTAA
AGAAAAAGTCGATTATTTACAAGAGAAATATGGTGATTTAATAACTGCAATTTACAATAG
TAGAGATACTGAATATTGCGATGAAATTAAACAACAATCAGCACAAGGAGGTGTAGATTT
AATTTTAAATACACTCTCTGGTGATTATTTATCTGCAAATTTCCGTTCACTTTCCCAAGT
TGGTAGAATTATGGATTTATCAGTCACTCAATTGGTTGAAAATGATTCATTAGATTTTTC
AAACTTTAAGTATCATGTTACTTACAGTACAATCGATTTAGAAAGAGCTACAACATATAA
TTCAAAAATCGT----------GAAATTAGAAAACTTTATTCTCTTTTCATCAATTGGTG
CTATCATTGGTGGTTCAAAACAATGTGCATATAGTAGTGCTAATTTAGTATTGGATTCAT
TATCAAACTATAGAAAGAGTATTGGTCTTGCTTCAACTTCAATTAATTGGGGTGGTTTGG
ATGCTGGTGGTGTTGCCGCCACTGATAAATCAGTCGCAAGTTTCTTAGAGGGTCAAGGTA
TTTTATTGGTTTCTTTATCAAAGATTTTAGGTTGTCTTGATTCAGTTTTCCAACCATCTA
ATTCCCATTTATCAAACTTTATGCTTTCAAGTTTCAATATTGATAATCTTTTATCTTCTG
CACCTCAAATGAAGAGAAAAATGGGTCACCACCTAACAAATTATAAAACTTCATCTGCTA
GCTCTGATGATTCATTGGGTGATTCTAGTTCTACTCAAGCAAAAGTCATTTCTACAATCT
CTGAATTATTATCAATTCATCCATCAAAATTAAATTTAGATACAAGATTAAAAGACTATG
GTATTGATTCTTTATTAACAGTACAATTAAAGAATTGGATAGATAAAGAATTTACAAAGA
ATTTATTCACTCATTTACAATTATCATCAAGTAGTATTAATAGTATCATTCAGAGAATTA
GTTCAAAATCAACTTCAACATCTACTCCCAATCCAACTAACACCACAAAACAAACCGCAA
CAACAAAAACATAAGTAGTTCAAACAACTCCAACTACATT
Length of connected seq. 1350
Full length Seq ID -
Full length Seq. -
Length of full length seq. -