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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
AFO259
Library AF
(Link to library)
Clone ID AFO259
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16382-1
Original site URL
Representative seq. ID AFO259E
(Link to Original site)
Representative DNA sequence 
>AFO259 (AFO259Q) /CSM/AF/AFO2-C/AFO259Q.Seq.d/
ATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAAA
AAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGGT
ATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTT
GGTCGTCCAAGACACACTGGNGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGGN
GATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTATT
GTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCGN
GTTGCTCCANAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCAAAT
AGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGCC
ATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGATTCA
GGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCACATGCTAT
TTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGA
ACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAAGAGAA
ATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCATCTGC
ATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAACGTTT
CCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTATTCA
TGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATATGG
TAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAATAA
AGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCACCAGAACG
NAAAAAC
sequence update 2001. 6. 9
Translated Amino Acid sequence 
lnnskdfnyililnk*tnkkknkkwmxkmfkl*llimvqvcvklvllvmmlhvlfshqll
vvqdtlxlwlvwvkrihmsxmklnqkevf*hlsiqlnmvlspigmiwkksgiilfimnfx
llxrniqfx*lklh*iqkqierk*LKSCLKLSTLQQCMLPFKLYYHSMPLVVPLVSLWIQ
VMVFPILYQFTKGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEK
LAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIH
ETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPER
KN
Translated Amino Acid sequence (All Frames) 
Frame A:
ikqlkrf*lyfnik*ink*kkk*kmdgedvqalvidngsgmckagfagddapravfpsiv
grprhtgvmvgmgqkdsyvgdeaqskrgiltlkypiehgivtnwddmekiwhhtfynelr
vapxehpvllteaplnpkanrekmtqimfetfntpamyvaiqavlslyasgrttgivmds
gdgvshtvpiyeglcittcyftfrfsws*sy*lhdenln*twllihnnc*krnr*ry*re
isircigf*s*nancsliicireii*itrwssyynw**tfplsrstfptiilgygvcwys
*nnl*fnhem*c*ys*rfiw*cciirwynyvpryc*sye*rinctctinnen*nycttrt
xk


Frame B:
lnnskdfnyililnk*tnkkknkkwmxkmfkl*llimvqvcvklvllvmmlhvlfshqll
vvqdtlxlwlvwvkrihmsxmklnqkevf*hlsiqlnmvlspigmiwkksgiilfimnfx
llxrniqfx*lklh*iqkqierk*LKSCLKLSTLQQCMLPFKLYYHSMPLVVPLVSLWIQ
VMVFPILYQFTKGYALPHAILRLDLAGRDLTDYMMKILTERGYSFTTTAEREIVRDIKEK
LAYVALDFEAEMQTAASSSALEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESAGIH
ETTYNSIMKCDVDIRKDLYGNVVLSGGTTMFPGIADRMNKELTALAPSTMKIKIIAPPER
KN


Frame C:
*ttqkiliif*y*inkqikkkikngwxrcssfsy**wfryv*swfcw**cstccfpincw
sskthwxygwygskgficrx*ssikkryfnt*vsn*twychqlg*ygknlasyfl**tsx
csxgtsssxn*ssiksksk*rendsnhv*nfqhssnvcchssciitlclwsyhwyrygfr
*wcfpyctnlrrvmhyhmlfyv*i*lvvillit**ks*lnvvthsqqllkeksleilkrn
*htlhwilklkcklqphhlh*rnhmnyqmvkllqlvmnvsvvqkhfsnhhswvwsllvfm
kqpiiqs*nvmlifvkiymvmlyyqvvqlcsqvlliv*ikn*lhlhhqq*klkllhhqnx
k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO259 (AFO259Q) /CSM/AF/AFO2-C/AFO259Q.Seq.d/                       1968   0.0
SFL261 (SFL261Q) /CSM/SF/SFL2-C/SFL261Q.Seq.d/                       1963   0.0
SFD790 (SFD790Q) /CSM/SF/SFD7-D/SFD790Q.Seq.d/                       1963   0.0
SFD319 (SFD319Q) /CSM/SF/SFD3-A/SFD319Q.Seq.d/                       1963   0.0
CHD357 (CHD357Q) /CSM/CH/CHD3-C/CHD357Q.Seq.d/                       1963   0.0
CFI221 (CFI221Q) /CSM/CF/CFI2-A/CFI221Q.Seq.d/                       1963   0.0
AFN507 (AFN507Q) /CSM/AF/AFN5-A/AFN507Q.Seq.d/                       1963   0.0
AFE346 (AFE346Q) /CSM/AF/AFE3-B/AFE346Q.Seq.d/                       1963   0.0
AFA723 (AFA723Q) /CSM/AF/AFA7-A/AFA723Q.Seq.d/                       1963   0.0
AFL134 (AFL134Q) /CSM/AF/AFL1-B/AFL134Q.Seq.d/                       1961   0.0
own update 2004.12.25
Homology vs DNA 

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

X03283|X03283.1 Dictyostelium discoideum gene for actin A3-S1.   825  0.0  2
AC116305|AC116305.2 Dictyostelium discoideum chromosome 2 map 1005175-1418323 strain AX4, complete sequence.   793  0.0  6
AC116956|AC116956.2 Dictyostelium discoideum chromosome 2 map 1418423-1684967 strain AX4, complete sequence.   728  0.0  6
AC115577|AC115577.2 Dictyostelium discoideum chromosome 2 map 4657875-4914984 strain AX4, complete sequence.   728  0.0  13
X03284|X03284.1 Dictyostelium discoideum gene for actin A3-S2.   706  0.0  2
X03282|X03282.1 dictyostelium discoideum gene for actin A12.     698  0.0  2
AC116986|AC116986.2 Dictyostelium discoideum chromosome 2 map 2234041-2567370 strain AX4, complete sequence.   674  0.0  6
M14146|M14146.1 D.discoideum actin 15 gene, complete cds.        611  0.0  2
AC115579|AC115579.2 Dictyostelium discoideum chromosome 2 map 4915084-5005461 strain AX4, complete sequence.   611  0.0  3
AC116957|AC116957.2 Dictyostelium discoideum chromosome 2 map 1685067-2090751 strain AX4, complete sequence.   603  0.0  5
dna update 2003. 9.19
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54H30) RecName: Full=Major actin; AltName: Full=Actin-1; AltNa...   347   0.0
(Q553U6) RecName: Full=Putative actin-22;  &AC116305_3(AC116305|...   347   0.0
B23412(B23412)actin 12 - slime mold (Dictyostelium discoideum)        341   0.0
ATDO(A93223;A92871;S67999;A03004)actin - slime mold (Dictyosteli...   345   0.0
(P02576) RecName: Full=Actin, plasmodial isoform;  &ATFY(A29044;...   345   0.0
X03282_1(X03282|pid:none) dictyostelium discoideum gene for acti...   339   0.0
(P07829) RecName: Full=Actin-3; AltName: Full=Actin-3-sub 1;          338   0.0
AY294152_1(AY294152|pid:none) Mayorella sp. JJP-2003 actin mRNA,...   339   0.0
AY294154_1(AY294154|pid:none) Thecamoeba similis actin mRNA, com...   338   0.0
AY294153_1(AY294153|pid:none) Platyamoeba placida actin mRNA, pa...   335   0.0
protein update 2009. 6.29
PSORT 

 psg: 0.82  gvh: 0.45  alm: 0.26  top: 0.53  tms: 0.07  mit: 0.41  mip: 0.10
 nuc: 0.00  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.38  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: mitochondrial
	 20.0 %: endoplasmic reticulum
	 16.0 %: Golgi
	 12.0 %: cytoplasmic
	 12.0 %: nuclear
	  8.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for AFO259 is mit
5' end seq. ID AFO259F
5' end seq. 
>AFO259F.Seq
ATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAAA
AAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGGT
ATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTT
GGTCGTCCAAGACACACTGGNGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGGN
GATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTATT
GTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCGN
GTTGCTCCANAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCAAAT
AGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGCC
ATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTAC----------
Length of 5' end seq. 518
3' end seq. ID AFO259Z
3' end seq. 
>AFO259Z.Seq
----------AATGTATGTTGCCATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTA
CCACTGGTATCGTTATGGATTCAGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAG
GGTTATGCATTACCACATGCTATTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGAT
TACATGATGAAAATCTTAACTGAACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAA
ATCGTTAGAGATATTAAAGAGAAATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATG
CAAACTGCAGCCTCATCATCTGCATTAGAGAAATCATATGAATTACCAGATGGTCAAGTT
ATTACAATTGGTAATGAACGTTTCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTG
GGTATGGAGTCTGCTGGTATTCATGAAACAACCTATAATTCAATCATGAAATGTGATGTT
GATATTCGTAAAGATTTATATGGTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCA
GGTATTGCTGATCGTATGAATAAAGAATTAACTGCACTTGCACCATCAACAATGAAAATT
AAAATTATTGCACCACCAGAACGNAAAAAC
Length of 3' end seq. 620
Connected seq. ID AFO259P
Connected seq. 
>AFO259P.Seq
ATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAAA
AAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGGT
ATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTT
GGTCGTCCAAGACACACTGGNGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGGN
GATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTATT
GTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCGN
GTTGCTCCANAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCAAAT
AGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGCC
ATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTAC----------AATGTATGTTGC
CATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGATTC
AGGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCACATGCTA
TTTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTG
AACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAAGAGA
AATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCATCTG
CATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAACGTT
TCCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTATTC
ATGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATATG
GTAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAATA
AAGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCACCAGAAC
GNAAAAAC
Length of connected seq. 1138
Full length Seq ID AFO259E
Full length Seq. 
>AFO259E.Seq
ATTAAACAACTCAAAAGATTTTAATTATATTTTAATATTAAATAAATAAACAAATAAAAA
AAAAAATAAAAAATGGATGGNGAAGATGTTCAAGCTTTAGTTATTGATAATGGTTCAGGT
ATGTGTAAAGCTGGTTTTGCTGGTGATGATGCTCCACGTGCTGTTTTCCCATCAATTGTT
GGTCGTCCAAGACACACTGGNGTTATGGTTGGTATGGGTCAAAAGGATTCATATGTCGGN
GATGAAGCTCAATCAAAAAGAGGTATTTTAACACTTAAGTATCCAATTGAACATGGTATT
GTCACCAATTGGGATGATATGGAAAAAATCTGGCATCATACTTTTTATAATGAACTTCGN
GTTGCTCCANAGGAACATCCAGTTCTNTTAACTGAAGCTCCATTAAATCCAAAAGCAAAT
AGAGAGAAAATGACTCAAATCATGTTTGAAACTTTCAACACTCCAGCAATGTATGTTGCC
ATTCAAGCTGTATTATCACTCTATGCCTCTGGTCGTACCACTGGTATCGTTATGGATTCA
GGTGATGGTGTTTCCCATACTGTACCAATTTACGAAGGGTTATGCATTACCACATGCTAT
TTTACGTTTAGATTTAGCTGGTCGTGATCTTACTGATTACATGATGAAAATCTTAACTGA
ACGTGGTTACTCATTCACAACAACTGCTGAAAGAGAAATCGTTAGAGATATTAAAGAGAA
ATTAGCATACGTTGCATTGGATTTTGAAGCTGAAATGCAAACTGCAGCCTCATCATCTGC
ATTAGAGAAATCATATGAATTACCAGATGGTCAAGTTATTACAATTGGTAATGAACGTTT
CCGTTGTCCAGAAGCACTTTTCCAACCATCATTCTTGGGTATGGAGTCTGCTGGTATTCA
TGAAACAACCTATAATTCAATCATGAAATGTGATGTTGATATTCGTAAAGATTTATATGG
TAATGTTGTATTATCAGGTGGTACAACTATGTTCCCAGGTATTGCTGATCGTATGAATAA
AGAATTAACTGCACTTGCACCATCAACAATGAAAATTAAAATTATTGCACCACCAGAACG
NAAAAAC
Length of full length seq. 1087