NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI594
Library VH
(Link to library)
Clone ID VHI594
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U12745-1|Contig-U15636-1
Original site URL
Representative seq. ID VHI594P
(Link to Original site)
Representative DNA sequence 
>VHI594 (VHI594Q) /CSM/VH/VHI5-D/VHI594Q.Seq.d/
AGTCATCACTTTACATCTCTCATTATTAATTCTACCTTATAATAAATATTAAATTATAGT
AATAAAAGAGAATTAATAAATTGTGTATTTGTTTGTGGGTGTATATGTGTAATTTTATAT
ATTAAAAAAAAAAXXXXXXXXXXTCACCAATCAATTGTAATCCAAATGAATTAAATTGGC
CATTACTTACAACTACAAAATCAGTTTNTGATGTTATGGGTGAAAATAGATTTGGTGTTG
AACAATCAACTTCAACTCCAACAGGTGATTGGGAAAGTGATGAAGATATTTTCAGTGAAG
GTAAATCACAACAACAATCATCACAACAACAACAACAACAACAACAAAAAGGTGATTGGG
AAGAAGATATTTTAATTGGAGATGGTAATAATGGTGGTGGTGATGATGGTGGTTGGGAAC
GTGATGATTTGAAAGGTTTAGAAAAAATTGGTACAGATGGATTTAATAATAAACAAAATG
ATCATGTTGCATTATTTGTACCACCACAACCAGGTCCATCATTCTCAATGATTTGGGCAA
GAAATTCACAATTTGCAGTTGATCATATTGCAGCAGGTAGTTTTGAAAGTGCAATGAATA
TTTTAAATAGTCAAATTGGTGCAGTCAATTTCGATCCAATCAAATCAATGTTTATGAATA
TCTTTATGGCTACT
sequence update 2002.10.25
Translated Amino Acid sequence 
shhftsliinstl**ILNYSNKRELINCVFVCGCICVILYIKKK---

---SPINCNPNELNWPLLTTTKSVXDVMGENRFGVEQSTSTPTGDWESDEDIFSEGKSQQ
QSSQQQQQQQQKGDWEEDILIGDGNNGGGDDGGWERDDLKGLEKIGTDGFNNKQNDHVAL
FVPPQPGPSFSMIWARNSQFAVDHIAAGSFESAMNILNSQIGAVNFDPIKSMFMNIFMAT
Translated Amino Acid sequence (All Frames) 
Frame A:
shhftsliinstl**ILNYSNKRELINCVFVCGCICVILYIKKK---

---SPINCNPNELNWPLLTTTKSVXDVMGENRFGVEQSTSTPTGDWESDEDIFSEGKSQQ
QSSQQQQQQQQKGDWEEDILIGDGNNGGGDDGGWERDDLKGLEKIGTDGFNNKQNDHVAL
FVPPQPGPSFSMIWARNSQFAVDHIAAGSFESAMNILNSQIGAVNFDPIKSMFMNIFMAT

Frame B:
vitlhlsllilpynky*iiviken**ivylfvgvyv*fyilkkk---

---hqsiviqmn*ighylqlqnqfxmlwvkidlvlnnqlqlqqvigkvmkifsvkvnhnn
nhhnnnnnnnkkvigkkif*lemvimvvvmmvvgnvmi*kv*kklvqmdliinkmimlhy
lyhhnqvhhsq*fgqeihnlqliilqqvvlkvq*if*ivklvqsisiqsnqcl*islwl

Frame C:
sslyishy*fyliinikl***krinklciclwvymcnfiy*kk---

---tnql*sk*iklaitynykisx*cyg*k*iwc*tinfnsnr*lgk**ryfq*r*ittt
iittttttttkr*lgrryfnwrw**www**wwlgt**ferfrknwyrwi***tk*sccii
cttttrsiilndlgkkftics*sycsr*f*kcneyfk*snwcsqfrsnqinvyeylygy
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI594 (VHI594Q) /CSM/VH/VHI5-D/VHI594Q.Seq.d/                        648   0.0
AFH715 (AFH715Q) /CSM/AF/AFH7-A/AFH715Q.Seq.d/                        636   0.0
AHO512 (AHO512Q) /CSM/AH/AHO5-A/AHO512Q.Seq.d/                        617   e-176
AHP260 (AHP260Q) /CSM/AH/AHP2-C/AHP260Q.Seq.d/                        478   e-134
VHM655 (VHM655Q) /CSM/VH/VHM6-C/VHM655Q.Seq.d/                        303   2e-81
AHO260 (AHO260Q) /CSM/AH/AHO2-C/AHO260Q.Seq.d/                        280   3e-74
VHE696 (VHE696Q) /CSM/VH/VHE6-D/VHE696Q.Seq.d/                        244   1e-63
SHL466 (SHL466Q) /CSM/SH/SHL4-C/SHL466Q.Seq.d/                        244   1e-63
SHK794 (SHK794Q) /CSM/SH/SHK7-D/SHK794Q.Seq.d/                        244   1e-63
SHH307 (SHH307Q) /CSM/SH/SHH3-A/SHH307Q.Seq.d/                        244   1e-63
own update 2004. 5.26
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442117) Dictyostelium discoideum cDNA clone:ddv48k23, 3' ...   648   0.0    2
(BJ356359) Dictyostelium discoideum cDNA clone:dda60g03, 3' ...   617   0.0    2
(BJ343468) Dictyostelium discoideum cDNA clone:dda16n03, 3' ...   599   e-167  1
(BJ356829) Dictyostelium discoideum cDNA clone:dda61g16, 3' ...   478   e-130  1
(BJ444528) Dictyostelium discoideum cDNA clone:ddv56m14, 3' ...   236   4e-76  2
(BJ356199) Dictyostelium discoideum cDNA clone:dda59g16, 3' ...   280   6e-71  1
(BJ423346) Dictyostelium discoideum cDNA clone:ddv48k23, 5' ...    82   3e-17  2
(BJ420948) Dictyostelium discoideum cDNA clone:ddv40o24, 5' ...    82   3e-17  2
(BJ335774) Dictyostelium discoideum cDNA clone:dda51c13, 5' ...    82   3e-17  2
(BJ397499) Dictyostelium discoideum cDNA clone:dds46d18, 5' ...    82   3e-17  2
dna update 2008.11.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55FR9) RecName: Full=Coatomer subunit alpha; AltName: Full=Alp...   305   1e-81
AK043479_1(AK043479|pid:none) Mus musculus 7 days neonate cerebe...    77   5e-13
(Q8CIE6) RecName: Full=Coatomer subunit alpha; AltName: Full=Alp...    77   5e-13
BC082785_1(BC082785|pid:none) Mus musculus coatomer protein comp...    77   5e-13
AK032071_1(AK032071|pid:none) Mus musculus adult male medulla ob...    77   6e-13
(Q96WV5) RecName: Full=Putative coatomer subunit alpha; AltName:...    76   8e-13
AC149637_4(AC149637|pid:none) Medicago truncatula clone mth2-181...    75   1e-12
BC169090_1(BC169090|pid:none) Rattus norvegicus coatomer protein...    75   1e-12
AC146745_23(AC146745|pid:none) Medicago truncatula clone mth2-53...    75   1e-12
AB385218_1(AB385218|pid:none) Synthetic construct DNA, clone: pF...    75   1e-12
protein update 2009. 7.13
PSORT 

 psg: 0.95  gvh: 0.56  alm: 0.18  top: 0.53  tms: 0.07  mit: 0.28  mip: 0.03
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.50  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 28.0 %: endoplasmic reticulum
	 20.0 %: cytoplasmic
	 16.0 %: Golgi
	 16.0 %: nuclear
	 12.0 %: mitochondrial
	  4.0 %: plasma membrane
	  4.0 %: vesicles of secretory system

>> prediction for VHI594 is end
5' end seq. ID VHI594F
5' end seq. 
>VHI594F.Seq
AGTCATCACTTTACATCTCTCATTATTAATTCTACCTTATAATAAATATTAAATTATAGT
AATAAAAGAGAATTAATAAATTGTGTATTTGTTTGTGGGTGTATATGTGTAATTTTATAT
ATTAAAAAAAAAANNNNNNNNNN
Length of 5' end seq. 143
3' end seq. ID VHI594Z
3' end seq. 
>VHI594Z.Seq
NNNNNNNNNNTCACCAATCAATTGTAATCCAAATGAATTAAATTGGCCATTACTTACAAC
TACAAAATCAGTTTNTGATGTTATGGGTGAAAATAGATTTGGTGTTGAACAATCAACTTC
AACTCCAACAGGTGATTGGGAAAGTGATGAAGATATTTTCAGTGAAGGTAAATCACAACA
ACAATCATCACAACAACAACAACAACAACAACAAAAAGGTGATTGGGAAGAAGATATTTT
AATTGGAGATGGTAATAATGGTGGTGGTGATGATGGTGGTTGGGAACGTGATGATTTGAA
AGGTTTAGAAAAAATTGGTACAGATGGATTTAATAATAAACAAAATGATCATGTTGCATT
ATTTGTACCACCACAACCAGGTCCATCATTCTCAATGATTTGGGCAAGAAATTCACAATT
TGCAGTTGATCATATTGCAGCAGGTAGTTTTGAAAGTGCAATGAATATTTTAAATAGTCA
AATTGGTGCAGTCAATTTCGATCCAATCAAATCAATGTTTATGAATATCTTTATGGCTAC
T
Length of 3' end seq. 541
Connected seq. ID VHI594P
Connected seq. 
>VHI594P.Seq
AGTCATCACTTTACATCTCTCATTATTAATTCTACCTTATAATAAATATTAAATTATAGT
AATAAAAGAGAATTAATAAATTGTGTATTTGTTTGTGGGTGTATATGTGTAATTTTATAT
ATTAAAAAAAAAA----------TCACCAATCAATTGTAATCCAAATGAATTAAATTGGC
CATTACTTACAACTACAAAATCAGTTTNTGATGTTATGGGTGAAAATAGATTTGGTGTTG
AACAATCAACTTCAACTCCAACAGGTGATTGGGAAAGTGATGAAGATATTTTCAGTGAAG
GTAAATCACAACAACAATCATCACAACAACAACAACAACAACAACAAAAAGGTGATTGGG
AAGAAGATATTTTAATTGGAGATGGTAATAATGGTGGTGGTGATGATGGTGGTTGGGAAC
GTGATGATTTGAAAGGTTTAGAAAAAATTGGTACAGATGGATTTAATAATAAACAAAATG
ATCATGTTGCATTATTTGTACCACCACAACCAGGTCCATCATTCTCAATGATTTGGGCAA
GAAATTCACAATTTGCAGTTGATCATATTGCAGCAGGTAGTTTTGAAAGTGCAATGAATA
TTTTAAATAGTCAAATTGGTGCAGTCAATTTCGATCCAATCAAATCAATGTTTATGAATA
TCTTTATGGCTACT
Length of connected seq. 664
Full length Seq ID -
Full length Seq. -
Length of full length seq. -