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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI580
Library VH
(Link to library)
Clone ID VHI580
Atlas ID -
NBRP ID
dictyBase ID
Link to Contig Contig-U12494-1
Original site URL
Representative seq. ID VHI580P
(Link to Original site)
Representative DNA sequence 
>VHI580 (VHI580Q) /CSM/VH/VHI5-D/VHI580Q.Seq.d/
AAATTGATGTGGTTTCTTCTATCTTAATTTTTTAAAATAAAAAGATTAAAATATTAAATT
AGGAAAATGCAGAGAGAATAGATTAATTTAATTAATTTGTGGTTGCTTTAGATTGAGAAG
AGTTATCACCATATGGACCACTATATGGATTTTTAGGGGTATCATCATAAGAATTCTCAC
GAGAACGACCTATACTACCAGATTGGTCAAATATTTGTCTTTCATAGTCATTATAGTTTT
CTGGAATATGAATTTCTAATGAACCGTCTGGTGAGGATCTAGTATACCAATAACCTTGTT
GTTGTGATAACCAATCTCTGTTTGTGTTGTAATTCTCATTGTTTTGATTATAATTTTGAT
TGTTTTCATTGGTTTGTCCATTTTGTGCTTCAAAGTATTCATTGTTTTCATTTACTTCAT
TTCCACCATTTTGCATTTCCATAACGTTATCTACATTGGTTGCTGAAAAGTTTGGACCTG
ATCCCCAACCTGCACCATATGCACTACGTTTATTTCCATGTACTGATCCAGTATTACTAT
GTGATGTTGAAGATGTGGTTGTTGAAGATGTGGCTGATCCACCAGTTGTACTTGACCCAC
TAGTATGATGTCCACTAGTTAAAATTGTAATTGAGGCAAATTTTGGTTCXXXXXXXXXXT
GATGCTGTTGATGTANAAGCANATCCCCCCAGTTGNACCAGGCCCCACCTGTAGATTGAT
GGGTTATTACCGGNTAGTTGGCAATTGTAATTGTNGNTAATGCTGGGGGTGGGTGAGCTT
GTGCTGCCTCCATTTCCCATTATTACCATTATTTACCGCCTGATGAGGTTGATGTTGAAG
CAGATCCACCAGTTGTACCTGCTCCACTTGTAGATTGATGGTTATTACCGCCAGTTGCAA
TTGTAATTGTTGCCAATGCTGGTTCTGGTGAACTTGTGCTGCCTCCGTTTCCATTATTGC
CTGTATTACCATTATTACCAGTATTACCATTATGTCCGGTATTACCGCCTGATGATGTTG
ATGTTGAAGCAGATCCACCTATTGTACCTGTTCCACCTGTAGATTGATGGTTATTGCCAC
CTGTTGCAATTGTAATTGTTGCTAATGCTGGTTCTGGCGAACTTGTGCTTCCTCCATTTC
CATTGTTGCCAGTATTACCGGTATTACCATTACCAGTATTACCATTGTTGCCTGTATTAC
CATTATTACCATTACCAGTATTACCAGTATTACCAGTATTACCGTTATTACCAGTATTAC
CATTATTACCAGTATTACCATTATTACCAGTATTACCAGTATTACCATTATTACCAGTAT
TACCATTATTACCAGTATTACCATTACTACCAGTATTACCATTGCCATTATTACCATTAC
CGTTATTCTTTGGCACACATCTCAATGTTTTTTTGACAACAATGAGTATTTAAACAACCA
TCATCTTGGGATAATTTGCAATCCATACCTTTACTTTCACAATCAAGTTGAGAACATGAT
GTAACTATAATAAAATTAAATATTATTAATAAAATCATTAACAATATTATTTAAG
sequence update 2002.10.25
Translated Amino Acid sequence 
idvvssilif*nkkikiln*enaerid*fn*fvvald*eelspygplygflgvss*efsr
erpilpdwsnicls*sl*fsgi*isnepsgedlvyq*pcccdnqslfvl*fslf*l*f*l
fslvcpfcaskyslfsftsfppfcisitlstlvaekfgpdpqpapyalrlfpctdpvllc
dvedvvvedvadppvvldplv*CPLVKIVIEANFG---

---MLLMXKXIPPVXPGPTCRLMGYYRXVGNCNCX*cwgwvslcclhfpllplfta**g*
c*srstsctcstcrlmvitascncnccqcwfw*tcaasvsiiacitiitsitimsgita*
*c*c*srstyctcstcrlmviatccncncc*cwfwrtcassisivasitgititsitiva
citiititsitsitsitvitsitiitsitiitsitsitiitsitiitsitittsitiaii
titvilwhtsqcffdnneylnnhhlgiicnpylyfhnqvenmm*l**n*illiksltilf
k
Translated Amino Acid sequence (All Frames) 
Frame A:
klmwflls*ffkikrlky*irkmqre*inlinlwll*ieksyhhmdhymdf*gyhhknsh
endlyyqigqifvfhshysfleyeflmnrlvri*ytnnlvvvitnlclccnshcfdynfd
cfhwfvhfvlqsihcfhllhfhhfafp*rylhwllksldlipnlhhmhyvyfhvliqyyy
vmlkmwllkmwlihqlylth*ydvh*lkl*lrqilv---

---*cc*cxsxspqlxqappvd*wvitg*laivivxnaggg*acaasishyyhylppdev
dveadppvvpaplvd*wllppvaivivanagsgelvlppfpllpvlpllpvlplcpvlpp
ddvdveadppivpvppvd*wllppvaivivanagsgelvlppfpllpvlpvlplpvlpll
pvlpllplpvlpvlpvlpllpvlpllpvlpllpvlpvlpllpvlpllpvlpllpvlplpl
lplplffgthlnvflttmsi*ttiilg*faihtftftiklrt*cnynkikyy**nh*qyy
l

Frame B:
n*cgffylnflk*kd*niklgkcrenrli*licgcfrlrrvitiwttiwifrgiiirilt
rttyttrlvkylsfiviivfwnmnf**tvw*gssipitlll**pisvcvvilivliiili
vfiglsilcfkvfivfiyfistilhfhnviyigc*kvwt*sptcticttfismy*ssitm
*c*rcgc*rcg*stsct*ptsmmsts*ncn*gkfwf---

---davdvxaxppsxtrphl*idgllpxswql*lxxmlgvgelvlppfpiitiiyrlmrl
mlkqihqlyllhl*idgyyrqlql*llpmlvlvnlcclrfhyclyyhyyqyyhyvryyrl
mmlmlkqihllylfhl*idgychllql*lllmlvlanlcflhfhccqyyryyhyqyyhcc
lyyhyyhyqyyqyyqyyryyqyyhyyqyyhyyqyyqyyhyyqyyhyyqyyhyyqyyhchy
yhyryslahismff*qq*vfkqpsswdnlqsipllsqss*ehdvtiiklniinkiinnii
*

Frame C:
idvvssilif*nkkikiln*enaerid*fn*fvvald*eelspygplygflgvss*efsr
erpilpdwsnicls*sl*fsgi*isnepsgedlvyq*pcccdnqslfvl*fslf*l*f*l
fslvcpfcaskyslfsftsfppfcisitlstlvaekfgpdpqpapyalrlfpctdpvllc
dvedvvvedvadppvvldplv*CPLVKIVIEANFG---

---MLLMXKXIPPVXPGPTCRLMGYYRXVGNCNCX*cwgwvslcclhfpllplfta**g*
c*srstsctcstcrlmvitascncnccqcwfw*tcaasvsiiacitiitsitimsgita*
*c*c*srstyctcstcrlmviatccncncc*cwfwrtcassisivasitgititsitiva
citiititsitsitsitvitsitiitsitiitsitsitiitsitiitsitittsitiaii
titvilwhtsqcffdnneylnnhhlgiicnpylyfhnqvenmm*l**n*illiksltilf
k
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI580 (VHI580Q) /CSM/VH/VHI5-D/VHI580Q.Seq.d/                       1998   0.0
SSH603 (SSH603Q) /CSM/SS/SSH6-A/SSH603Q.Seq.d/                         48   4e-04
SSM412 (SSM412Q) /CSM/SS/SSM4-A/SSM412Q.Seq.d/                         46   0.002
SSH269 (SSH269Q) /CSM/SS/SSH2-C/SSH269Q.Seq.d/                         46   0.002
VHL886 (VHL886Q) /CSM/VH/VHL8-D/VHL886Q.Seq.d/                         42   0.025
SSH622 (SSH622Q) /CSM/SS/SSH6-A/SSH622Q.Seq.d/                         42   0.025
SSA347 (SSA347Q) /CSM/SS/SSA3-B/SSA347Q.Seq.d/                         40   0.100
CFG851 (CFG851Q) /CSM/CF/CFG8-C/CFG851Q.Seq.d/                         40   0.100
AHN271 (AHN271Q) /CSM/AH/AHN2-C/AHN271Q.Seq.d/                         40   0.100
VFK380 (VFK380Q) /CSM/VF/VFK3-D/VFK380Q.Seq.d/                         38   0.39
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ442135) Dictyostelium discoideum cDNA clone:ddv48o19, 3' ...   940   0.0    3
(BJ423365) Dictyostelium discoideum cDNA clone:ddv48o19, 5' ...   523   0.0    3
(AY428796) Dictyostelium discoideum DIF insensitive mutant A...    52   0.008  3
(AF482758) Cowpox virus strain Brighton Red, complete genome.      52   0.065  1
(EJ025038) 1095454016918 Global-Ocean-Sampling_GS-26-01-01-1...    52   0.065  1
(CW922586) EDCAT58TR A. castellanii, 6-8 kb library from tot...    52   0.065  1
(ER580667) 1093015837467 Global-Ocean-Sampling_GS-36-01-01-2...    40   0.19   2
(EL428367) CHCM7649.b1_B18.ab1 CHC(LMS) Texas blueweed Helia...    40   0.19   2
(AC217121) Zea mays chromosome 4 clone CH201-484A20; ZMMBBc0...    50   0.26   1
(U00038) Caenorhabditis elegans cosmid T21D11, complete sequ...    48   1.0    1
dna update 2008.11.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q04503) RecName: Full=Prespore protein Dp87; Flags: Precursor;        37   1.5
D13973_1(D13973|pid:none) Dictyostelium discoideum gene for Dp87...    37   1.5
protein update 2009. 3.11
PSORT 

 psg: 0.90  gvh: 0.56  alm: 0.36  top: 0.40  tms: 0.00  mit: 0.29  mip: 0.00
 nuc: 0.00  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 40.0 %: cytoplasmic
	 28.0 %: nuclear
	 16.0 %: extracellular, including cell wall
	 12.0 %: mitochondrial
	  4.0 %: vacuolar

>> prediction for VHI580 is cyt
5' end seq. ID VHI580F
5' end seq. 
>VHI580F.Seq
AAATTGATGTGGTTTCTTCTATCTTAATTTTTTAAAATAAAAAGATTAAAATATTAAATT
AGGAAAATGCAGAGAGAATAGATTAATTTAATTAATTTGTGGTTGCTTTAGATTGAGAAG
AGTTATCACCATATGGACCACTATATGGATTTTTAGGGGTATCATCATAAGAATTCTCAC
GAGAACGACCTATACTACCAGATTGGTCAAATATTTGTCTTTCATAGTCATTATAGTTTT
CTGGAATATGAATTTCTAATGAACCGTCTGGTGAGGATCTAGTATACCAATAACCTTGTT
GTTGTGATAACCAATCTCTGTTTGTGTTGTAATTCTCATTGTTTTGATTATAATTTTGAT
TGTTTTCATTGGTTTGTCCATTTTGTGCTTCAAAGTATTCATTGTTTTCATTTACTTCAT
TTCCACCATTTTGCATTTCCATAACGTTATCTACATTGGTTGCTGAAAAGTTTGGACCTG
ATCCCCAACCTGCACCATATGCACTACGTTTATTTCCATGTACTGATCCAGTATTACTAT
GTGATGTTGAAGATGTGGTTGTTGAAGATGTGGCTGATCCACCAGTTGTACTTGACCCAC
TAGTATGATGTCCACTAGTTAAAATTGTAATTGAGGCAAATTTTGGTTCNNNNNNNNNN
Length of 5' end seq. 659
3' end seq. ID VHI580Z
3' end seq. 
>VHI580Z.Seq
NNNNNNNNNNTGATGCTGTTGATGTANAAGCANATCCCCCCAGTTGNACCAGGCCCCACC
TGTAGATTGATGGGTTATTACCGGNTAGTTGGCAATTGTAATTGTNGNTAATGCTGGGGG
TGGGTGAGCTTGTGCTGCCTCCATTTCCCATTATTACCATTATTTACCGCCTGATGAGGT
TGATGTTGAAGCAGATCCACCAGTTGTACCTGCTCCACTTGTAGATTGATGGTTATTACC
GCCAGTTGCAATTGTAATTGTTGCCAATGCTGGTTCTGGTGAACTTGTGCTGCCTCCGTT
TCCATTATTGCCTGTATTACCATTATTACCAGTATTACCATTATGTCCGGTATTACCGCC
TGATGATGTTGATGTTGAAGCAGATCCACCTATTGTACCTGTTCCACCTGTAGATTGATG
GTTATTGCCACCTGTTGCAATTGTAATTGTTGCTAATGCTGGTTCTGGCGAACTTGTGCT
TCCTCCATTTCCATTGTTGCCAGTATTACCGGTATTACCATTACCAGTATTACCATTGTT
GCCTGTATTACCATTATTACCATTACCAGTATTACCAGTATTACCAGTATTACCGTTATT
ACCAGTATTACCATTATTACCAGTATTACCATTATTACCAGTATTACCAGTATTACCATT
ATTACCAGTATTACCATTATTACCAGTATTACCATTACTACCAGTATTACCATTGCCATT
ATTACCATTACCGTTATTCTTTGGCACACATCTCAATGTTTTTTTGACAACAATGAGTAT
TTAAACAACCATCATCTTGGGATAATTTGCAATCCATACCTTTACTTTCACAATCAAGTT
GAGAACATGATGTAACTATAATAAAATTAAATATTATTAATAAAATCATTAACAATATTA
TTTAAG
Length of 3' end seq. 906
Connected seq. ID VHI580P
Connected seq. 
>VHI580P.Seq
AAATTGATGTGGTTTCTTCTATCTTAATTTTTTAAAATAAAAAGATTAAAATATTAAATT
AGGAAAATGCAGAGAGAATAGATTAATTTAATTAATTTGTGGTTGCTTTAGATTGAGAAG
AGTTATCACCATATGGACCACTATATGGATTTTTAGGGGTATCATCATAAGAATTCTCAC
GAGAACGACCTATACTACCAGATTGGTCAAATATTTGTCTTTCATAGTCATTATAGTTTT
CTGGAATATGAATTTCTAATGAACCGTCTGGTGAGGATCTAGTATACCAATAACCTTGTT
GTTGTGATAACCAATCTCTGTTTGTGTTGTAATTCTCATTGTTTTGATTATAATTTTGAT
TGTTTTCATTGGTTTGTCCATTTTGTGCTTCAAAGTATTCATTGTTTTCATTTACTTCAT
TTCCACCATTTTGCATTTCCATAACGTTATCTACATTGGTTGCTGAAAAGTTTGGACCTG
ATCCCCAACCTGCACCATATGCACTACGTTTATTTCCATGTACTGATCCAGTATTACTAT
GTGATGTTGAAGATGTGGTTGTTGAAGATGTGGCTGATCCACCAGTTGTACTTGACCCAC
TAGTATGATGTCCACTAGTTAAAATTGTAATTGAGGCAAATTTTGGTTC----------T
GATGCTGTTGATGTANAAGCANATCCCCCCAGTTGNACCAGGCCCCACCTGTAGATTGAT
GGGTTATTACCGGNTAGTTGGCAATTGTAATTGTNGNTAATGCTGGGGGTGGGTGAGCTT
GTGCTGCCTCCATTTCCCATTATTACCATTATTTACCGCCTGATGAGGTTGATGTTGAAG
CAGATCCACCAGTTGTACCTGCTCCACTTGTAGATTGATGGTTATTACCGCCAGTTGCAA
TTGTAATTGTTGCCAATGCTGGTTCTGGTGAACTTGTGCTGCCTCCGTTTCCATTATTGC
CTGTATTACCATTATTACCAGTATTACCATTATGTCCGGTATTACCGCCTGATGATGTTG
ATGTTGAAGCAGATCCACCTATTGTACCTGTTCCACCTGTAGATTGATGGTTATTGCCAC
CTGTTGCAATTGTAATTGTTGCTAATGCTGGTTCTGGCGAACTTGTGCTTCCTCCATTTC
CATTGTTGCCAGTATTACCGGTATTACCATTACCAGTATTACCATTGTTGCCTGTATTAC
CATTATTACCATTACCAGTATTACCAGTATTACCAGTATTACCGTTATTACCAGTATTAC
CATTATTACCAGTATTACCATTATTACCAGTATTACCAGTATTACCATTATTACCAGTAT
TACCATTATTACCAGTATTACCATTACTACCAGTATTACCATTGCCATTATTACCATTAC
CGTTATTCTTTGGCACACATCTCAATGTTTTTTTGACAACAATGAGTATTTAAACAACCA
TCATCTTGGGATAATTTGCAATCCATACCTTTACTTTCACAATCAAGTTGAGAACATGAT
GTAACTATAATAAAATTAAATATTATTAATAAAATCATTAACAATATTATTTAAG
Length of connected seq. 1545
Full length Seq ID -
Full length Seq. -
Length of full length seq. -