>AFO256 (AFO256Q) /CSM/AF/AFO2-C/AFO256Q.Seq.d/
XXXXXXXXXXTANATTTTTTTTTAAAATCTTAANATTTAAANAAAAAAAAAAAAATCATT
AAATTTTAAAAGTAAAAATTGTTGTATTTATATAAATATATATATTTAATATTGTAATAA
TAATAAAAAAA

---iff*nlxi*XKKKKSLNFKSKNCCIYINIYI*ycnnnkk

Frame A:
---xfffkilxfkxkkknh*ilkvkivvfi*iyifniviiikk


Frame B:
---xfflks*xlxkkkkiikf*k*kllylykyiylil****k


Frame C:
---iff*nlxi*XKKKKSLNFKSKNCCIYINIYI*ycnnnkk

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AFO256 (AFO256Q) /CSM/AF/AFO2-C/AFO256Q.Seq.d/                         50   8e-06
SLK675 (SLK675Q) /CSM/SL/SLK6-D/SLK675Q.Seq.d/                         42   0.002
SLI863 (SLI863Q) /CSM/SL/SLI8-C/SLI863Q.Seq.d/                         42   0.002
SHJ355 (SHJ355Q) /CSM/SH/SHJ3-C/SHJ355Q.Seq.d/                         42   0.002
SHJ174 (SHJ174Q) /CSM/SH/SHJ1-D/SHJ174Q.Seq.d/                         42   0.002
SHD121 (SHD121Q) /CSM/SH/SHD1-A/SHD121Q.Seq.d/                         42   0.002
SHC305 (SHC305Q) /CSM/SH/SHC3-A/SHC305Q.Seq.d/                         42   0.002
SFL313 (SFL313Q) /CSM/SF/SFL3-A/SFL313Q.Seq.d/                         42   0.002
SFJ180 (SFJ180Q) /CSM/SF/SFJ1-D/SFJ180Q.Seq.d/                         42   0.002
CHQ177 (CHQ177Q) /CSM/CH/CHQ1-D/CHQ177Q.Seq.d/                         42   0.002

                                                               Score     E
Sequences producing significant alignments:                    (bits)  Value  N

AZ549565|AZ549565.1 ENTGD95TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42  1.5  1
AZ528446|AZ528446.1 ENTCI79TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42  1.5  1
AZ688547|AZ688547.1 ENTIN90TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42  1.5  1
BH148090|BH148090.1 ENTQM09TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42  1.5  1
BH131557|BH131557.1 ENTOL83TF Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42  1.5  1
AZ545075|AZ545075.1 ENTEW90TR Entamoeba histolytica Sheared DNA Entamoeba histolytica genomic, DNA sequence.    42  1.5  1
AL035686|AL035686.15 Human DNA sequence from clone RP5-965N20 on chromosome 20q13.13-13.2 Contains ESTs, STSs and GSSs.    40  5.8  1
BX510917|BX510917.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone CH211-250O15.    40  5.8  1
BX510366|BX510366.3 Zebrafish DNA sequence *** SEQUENCING IN PROGRESS *** from clone DKEY-189P24.    40  5.8  1
BB247290|BB247290.2 Mus musculus 7 days neonate cerebellum cDNA, RIKEN full-length enriched library, clone:A730018G13, 3' end partial sequence.    40  5.8  1

 ***** No hits found ******

Lambda     K      H
   0.318    0.134    0.401

Gapped
Lambda     K      H
   0.267   0.0410    0.140

Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 3039690
Number of Hits to DB: 14,257,166

 psg: 0.84  gvh: 0.02  alm: 0.54  top: 0.53  tms: 0.00  mit: 0.37  mip: 0.00
 nuc: 0.16  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: nuclear
	 20.0 %: cytoplasmic
	  8.0 %: mitochondrial
	  4.0 %: cytoskeletal
	  4.0 %: Golgi

>> prediction for AFO256 is nuc

>AFO256Z.Seq
----------TANATTTTTTTTTAAAATCTTAANATTTAAANAAAAAAAAAAAAATCATT
AAATTTTAAAAGTAAAAATTGTTGTATTTATATAAATATATATATTTAATATTGTAATAA
TAATAAAAAAA