NBDC - アーカイブトップ - ヘルプ
データベースの説明 | データ項目の説明 | 利用許諾 | ヘルプ
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
NBDC - LSDB Archive
Description of this DB | Description of data items | Download | License
Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI571
Library VH
(Link to library)
Clone ID VHI571
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16439-1
Original site URL
Representative seq. ID VHI571P
(Link to Original site)
Representative DNA sequence 
>VHI571 (VHI571Q) /CSM/VH/VHI5-C/VHI571Q.Seq.d/
CATTATCTCATGAAACAATGAAAAAAATTACATCAACTTCAGTTTTAGTTGTAGGTTTAC
AAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTGTTAAATCTGTTACAT
TGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAATTTTATTTTTCACCAG
AACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAGTTGTAGATTTAAATA
ATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAATTCTTAAAGAAATTCA
ATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAGTGAATGAATTTTGTC
ATGCAAATAAAATTCATTTCATTAGTGTTGAAACCAGAGGTGTTTTTGGTCAATTATTCA
ATGATTTTGGTGAACAATTTACAATCACCGATACAAATGGTGAGAATCCAAATGCTTATA
TGATCAGTTCAATCAGTCAAGATAAAGAAGGTATTGTAACCGTTGTTGAAGAACAAAAAT
TACAACTTTTAGATGGTGATCTTGTTACATTCAAAGAAGTCAATGGTATGTCAGCTCTTA
ATGATCTTCCAXXXXXXXXXXAACCAGCTCCATCTTCAAACACTCAACAAGCTGGTGGTG
ATGCTGAAGATGATCAATGTGATACCATTTTATCNCAACTTCCACAACCAAGTGAAATGG
CTGGTTACAAAATTAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCG
ATTTCATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATA
AACATAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCG
TAGTTGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGA
AATATAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTG
CCGCCCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATG
TTGATGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGG
ATATTTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAA
CTAAAGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAAC
CATTACCAAAAGATAAGAAATATT
sequence update 2002.10.25
Translated Amino Acid sequence 
LSHETMKKITSTSVLVVGLQGLGIEIVKDLSLAGVKSVTLYDKELVEIKDLSSQFYFSPE
QVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPLALQLKVNEFCH
ANKIHFISVETRGVFGQLFNDFGEQFTITDTNGENPNAYMISSISQDKEGIVTVVEEQKL
QLLDGDLVTFKEVNGMSALNDLP---

---PAPSSNTQQAGGDAEDDQCDTILSQLPQPSEMAGYKINSIQFEKDDDTNHHIDFITA
TSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVVAGFVCIELIKVIQNKALEKYKST
FMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLFEKKHGLDISML
SCKVTLLYALFTDKKTKEERLKMKISQLYETLSKKPLPKDKKY
Translated Amino Acid sequence (All Frames) 
Frame A:
hylmkq*kklhqlqf*l*vykvlvlkllki*v*qvlnllhcmikn*lklkicllnfifhq
nklvklveqmhvskkl*i*iimlelmyimen*vmns*rnsml*y*pinh*hynsk*mnfv
mqikfislvlkpevflvnysmilvnnlqspiqmvriqmli*svqsvkikkvl*pllknkn
ynf*mvillhskksmvcqllmif---

---nqlhlqtlnklvvmlkminvipfyxnfhnqvkwlvtkltlfnlkrmmipiiisislp
lpqi*elptmqsvqlinirpkvspvksfql*lpllp*llvsfvsn*lklfkiklwrnira
pl*i*vshslvslnqlppqrtkfvkaglghfgivsmlmvislsrsf*islkrnmvwifqc
slvklhfymlsslirklkrkd*r*kshnsmkpsqrnhyqkirni

Frame B:
iis*nneknyinfsfscrftrswy*nc*rfkfsrc*icyiv**ris*n*rfvfsilfftr
tsw*sw*srcmfpkscrfk*lc*n*ct*wrik**ilkeiqccsisqstisittqse*ils
ck*nsfh*c*nqrcfwsiiq*fw*tiynhrykw*eskclydqfnqsr*rrycnrc*rtki
ttfrw*scyiqrsqwyvss**ss---

---tssifkhstsww*c*r*sm*yhfixtsttk*ngwlqn*lysi*kg**yqssyrfhyr
ylkfksyqlcnqss**t*dqryrr*nhssfsyhycrscwfrlyrid*sysk*sfgei*eh
lyefrypilwfr*tncrpkeqns*rldldtlgsfrc*w*yhsqgvfrsl*ketwfgyfna
ll*sytficslh**en*rgkikdenlttl*nplketitkr*ei

Frame C:
LSHETMKKITSTSVLVVGLQGLGIEIVKDLSLAGVKSVTLYDKELVEIKDLSSQFYFSPE
QVGKVGRADACFQKVVDLNNYVRIDVHNGELSDEFLKKFNVVVLANQPLALQLKVNEFCH
ANKIHFISVETRGVFGQLFNDFGEQFTITDTNGENPNAYMISSISQDKEGIVTVVEEQKL
QLLDGDLVTFKEVNGMSALNDLP---

---PAPSSNTQQAGGDAEDDQCDTILSQLPQPSEMAGYKINSIQFEKDDDTNHHIDFITA
TSNLRATNYAISPADKHKTKGIAGKIIPALVTTTAVVAGFVCIELIKVIQNKALEKYKST
FMNLGIPFFGFVEPIAAPKNKIREGWTWTLWDRFDVDGDITLKEFLDLFEKKHGLDISML
SCKVTLLYALFTDKKTKEERLKMKISQLYETLSKKPLPKDKKY
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI571 (VHI571Q) /CSM/VH/VHI5-C/VHI571Q.Seq.d/                       2438   0.0
VHP750 (VHP750Q) /CSM/VH/VHP7-C/VHP750Q.Seq.d/                       1308   0.0
VHO562 (VHO562Q) /CSM/VH/VHO5-C/VHO562Q.Seq.d/                       1308   0.0
VHN723 (VHN723Q) /CSM/VH/VHN7-A/VHN723Q.Seq.d/                       1308   0.0
VHM844 (VHM844Q) /CSM/VH/VHM8-B/VHM844Q.Seq.d/                       1308   0.0
VHI150 (VHI150Q) /CSM/VH/VHI1-C/VHI150Q.Seq.d/                       1308   0.0
VHB744 (VHB744Q) /CSM/VH/VHB7-B/VHB744Q.Seq.d/                       1308   0.0
VHA440 (VHA440Q) /CSM/VH/VHA4-B/VHA440Q.Seq.d/                       1308   0.0
VHA328 (VHA328Q) /CSM/VH/VHA3-B/VHA328Q.Seq.d/                       1308   0.0
VHA239 (VHA239Q) /CSM/VH/VHA2-B/VHA239Q.Seq.d/                       1308   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ446376) Dictyostelium discoideum cDNA clone:ddv62d13, 3' ...  1308   0.0    1
(BJ445783) Dictyostelium discoideum cDNA clone:ddv60k15, 3' ...  1308   0.0    1
(BJ445022) Dictyostelium discoideum cDNA clone:ddv58n05, 3' ...  1308   0.0    1
(BJ444564) Dictyostelium discoideum cDNA clone:ddv56d20, 3' ...  1308   0.0    1
(BJ442044) Dictyostelium discoideum cDNA clone:ddv48m17, 3' ...  1308   0.0    1
(BJ441686) Dictyostelium discoideum cDNA clone:ddv47c13, 3' ...  1308   0.0    1
(BJ437464) Dictyostelium discoideum cDNA clone:ddv34h11, 3' ...  1308   0.0    1
(BJ436536) Dictyostelium discoideum cDNA clone:ddv31p10, 3' ...  1308   0.0    1
(BJ436527) Dictyostelium discoideum cDNA clone:ddv31o07, 3' ...  1308   0.0    1
(BJ436518) Dictyostelium discoideum cDNA clone:ddv31m10, 3' ...  1308   0.0    1
dna update 2008.11.14
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q55C16) RecName: Full=Ubiquitin-like modifier-activating enzyme...   448   e-124
EF468345_1(EF468345|pid:none) Pisum sativum ubiquitin activating...   239   2e-61
CR382138_755(CR382138|pid:none) Debaryomyces hansenii strain CBS...   231   3e-59
AE017341_129(AE017341|pid:none) Cryptococcus neoformans var. neo...   229   2e-58
BX908809_46(BX908809|pid:none) Neurospora crassa DNA linkage gro...   224   5e-57
CP000496_820(CP000496|pid:none) Pichia stipitis CBS 6054 chromos...   224   7e-57
FN392321_874(FN392321|pid:none) Pichia pastoris GS115 chromosome...   223   1e-56
(P22515) RecName: Full=Ubiquitin-activating enzyme E1 1;  &S3804...   219   1e-55
AB035495_1(AB035495|pid:none) Carassius auratus mRNA for ubiquit...   217   6e-55
AM270347_41(AM270347|pid:none) Aspergillus niger contig An15c020...   216   1e-54
protein update 2009. 7.13
PSORT 

 psg: 0.92  gvh: 0.52  alm: 0.27  top: 0.43  tms: 0.07  mit: 0.28  mip: 0.00
 nuc: 0.03  erl: 0.00  erm: 0.60  pox: 0.00  px2: 0.50  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 1.00  tyr: 0.00  leu: 0.02  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 1.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 0.00 

	 32.0 %: mitochondrial
	 20.0 %: cytoplasmic
	 16.0 %: nuclear
	 12.0 %: endoplasmic reticulum
	  8.0 %: cytoskeletal
	  8.0 %: Golgi
	  4.0 %: vacuolar

>> prediction for VHI571 is mit
5' end seq. ID VHI571F
5' end seq. 
>VHI571F.Seq
CATTATCTCATGAAACAATGAAAAAAATTACATCAACTTCAGTTTTAGTTGTAGGTTTAC
AAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTGTTAAATCTGTTACAT
TGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAATTTTATTTTTCACCAG
AACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAGTTGTAGATTTAAATA
ATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAATTCTTAAAGAAATTCA
ATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAGTGAATGAATTTTGTC
ATGCAAATAAAATTCATTTCATTAGTGTTGAAACCAGAGGTGTTTTTGGTCAATTATTCA
ATGATTTTGGTGAACAATTTACAATCACCGATACAAATGGTGAGAATCCAAATGCTTATA
TGATCAGTTCAATCAGTCAAGATAAAGAAGGTATTGTAACCGTTGTTGAAGAACAAAAAT
TACAACTTTTAGATGGTGATCTTGTTACATTCAAAGAAGTCAATGGTATGTCAGCTCTTA
ATGATCTTCCANNNNNNNNNN
Length of 5' end seq. 621
3' end seq. ID VHI571Z
3' end seq. 
>VHI571Z.Seq
NNNNNNNNNNAACCAGCTCCATCTTCAAACACTCAACAAGCTGGTGGTGATGCTGAAGAT
GATCAATGTGATACCATTTTATCNCAACTTCCACAACCAAGTGAAATGGCTGGTTACAAA
ATTAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCGATTTCATTACC
GCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATAAACATAAGACC
AAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCGTAGTTGCTGGT
TTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGAAATATAAGAGC
ACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTGCCGCCCCAAAG
AACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATGTTGATGGTGAT
ATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGGATATTTCAATG
CTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAACTAAAGAGGAA
AGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAACCATTACCAAAA
GATAAGAAATATT
Length of 3' end seq. 673
Connected seq. ID VHI571P
Connected seq. 
>VHI571P.Seq
CATTATCTCATGAAACAATGAAAAAAATTACATCAACTTCAGTTTTAGTTGTAGGTTTAC
AAGGTCTTGGTATTGAAATTGTTAAAGATTTAAGTTTAGCAGGTGTTAAATCTGTTACAT
TGTATGATAAAGAATTAGTTGAAATTAAAGATTTGTCTTCTCAATTTTATTTTTCACCAG
AACAAGTTGGTAAAGTTGGTAGAGCAGATGCATGTTTCCAAAAAGTTGTAGATTTAAATA
ATTATGTTAGAATTGATGTACATAATGGAGAATTAAGTGATGAATTCTTAAAGAAATTCA
ATGTTGTAGTATTAGCCAATCAACCATTAGCATTACAACTCAAAGTGAATGAATTTTGTC
ATGCAAATAAAATTCATTTCATTAGTGTTGAAACCAGAGGTGTTTTTGGTCAATTATTCA
ATGATTTTGGTGAACAATTTACAATCACCGATACAAATGGTGAGAATCCAAATGCTTATA
TGATCAGTTCAATCAGTCAAGATAAAGAAGGTATTGTAACCGTTGTTGAAGAACAAAAAT
TACAACTTTTAGATGGTGATCTTGTTACATTCAAAGAAGTCAATGGTATGTCAGCTCTTA
ATGATCTTCCA----------AACCAGCTCCATCTTCAAACACTCAACAAGCTGGTGGTG
ATGCTGAAGATGATCAATGTGATACCATTTTATCNCAACTTCCACAACCAAGTGAAATGG
CTGGTTACAAAATTAACTCTATTCAATTTGAAAAGGATGATGATACCAATCATCATATCG
ATTTCATTACCGCTACCTCAAATTTAAGAGCTACCAACTATGCAATCAGTCCAGCTGATA
AACATAAGACCAAAGGTATCGCCGGTAAAATCATTCCAGCTTTAGTTACCACTACTGCCG
TAGTTGCTGGTTTCGTTTGTATCGAATTGATTAAAGTTATTCAAAATAAAGCTTTGGAGA
AATATAAGAGCACCTTTATGAATTTAGGTATCCCATTCTTTGGTTTCGTTGAACCAATTG
CCGCCCCAAAGAACAAAATTCGTGAAGGCTGGACTTGGACACTTTGGGATCGTTTCGATG
TTGATGGTGATATCACTCTCAAGGAGTTTTTAGATCTCTTTGAAAAGAAACATGGTTTGG
ATATTTCAATGCTCTCTTGTAAAGTTACACTTTTATATGCTCTCTTCACTGATAAGAAAA
CTAAAGAGGAAAGATTAAAGATGAAAATCTCACAACTCTATGAAACCCTCTCAAAGAAAC
CATTACCAAAAGATAAGAAATATT
Length of connected seq. 1274
Full length Seq ID -
Full length Seq. -
Length of full length seq. -