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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI548
Library VH
(Link to library)
Clone ID VHI548
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16292-1|Contig-U16400-1
Original site URL
Representative seq. ID VHI548P
(Link to Original site)
Representative DNA sequence 
>VHI548 (VHI548Q) /CSM/VH/VHI5-B/VHI548Q.Seq.d/
AATTTAAACAATATATAAATCAATTAAAATAAACACTCACTCGATAGTGTAAAAGAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTNAAAAAAAAAX
XXXXXXXXXAAGTGATACCTCTTTGAATAACACATTGGATGCATTAGATAAAATCAAACA
ATCAGCAGTGGCATCACGTCGTTTATTCGTCGTGGAGGTCATGGGTGCCCATTGTGGTTA
TTTGGCTGCAATGAGTTCTTTAACCTCAGGTGCTGAACGTTCATATATAATGGAAAGAGG
TATCACATTGAATACACTAACCAAGGATTTGGAAATGTTTGTTGAACGTTTCAAACGTGA
ACATAGAATTGGTCTAATTATTAAGAGTGAACTTGCTAGCAACACCTACTCAACTCATTT
CATTTATTCACTCTTCAAAGAAGAAGGTAAACATTTATTCGATGTCCGTGAATCTATACT
CGGTCATTTACAACAAGGTGGTACTCCTTCTGCAATTGATCGTATCTTTTCAACTCGTTT
AATGAATCATTATTATCAATTCTTGGAAAACGATCTCAAAGAACATGGTCATCTTCAAAT
GAATGGTTGTATTGGTTTCATTGATGGTGGTATTCATTACACTCCAATGCAAGAAATGAT
CGAAGAAATGTCTGATAAATTTAGACGTCCTCGTTCCCAATGGTGGATGGATTTGGTTGA
AACCTCTCAAAATATCTCTGTTTTCCT
sequence update 2002.10.25
Translated Amino Acid sequence 
nlnni*in*NKHSLDSVKEKKKKKKKKKKKKKKXXFXKK---

---SDTSLNNTLDALDKIKQSAVASRRLFVVEVMGAHCGYLAAMSSLTSGAERSYIMERG
ITLNTLTKDLEMFVERFKREHRIGLIIKSELASNTYSTHFIYSLFKEEGKHLFDVRESIL
GHLQQGGTPSAIDRIFSTRLMNHYYQFLENDLKEHGHLQMNGCIGFIDGGIHYTPMQEMI
EEMSDKFRRPRSQWWMDLVETSQNISVF
Translated Amino Acid sequence (All Frames) 
Frame A:
nlnni*in*NKHSLDSVKEKKKKKKKKKKKKKKXXFXKK---

---k*ylfe*higcir*nqtissgitsfirrgghgcplwlfgcneffnlrc*tfiyngkr
yhieytnqgfgnvc*tfqt*t*nwsny*e*tc*qhllnsfhlftlqrrr*tfircp*iyt
rsfttrwysfcn*sylfnsfnesllsilgkrsqrtwsssnewlywfh*wwyslhsnarnd
rrnv**i*tssfpmvdgfg*nlskylcfp

Frame B:
i*tiyksikinthsiv*kkkkkkkkkkkkkkkkxffxkk---

---SDTSLNNTLDALDKIKQSAVASRRLFVVEVMGAHCGYLAAMSSLTSGAERSYIMERG
ITLNTLTKDLEMFVERFKREHRIGLIIKSELASNTYSTHFIYSLFKEEGKHLFDVRESIL
GHLQQGGTPSAIDRIFSTRLMNHYYQFLENDLKEHGHLQMNGCIGFIDGGIHYTPMQEMI
EEMSDKFRRPRSQWWMDLVETSQNISVF

Frame C:
fkqyinqlk*tltr*ckrkkkkkkkkkkkkkkkxfxkkk---

---vipl*ithwmh*iksnnqqwhhvvysswrswvpivviwlq*vl*pqvlnvhi*wkev
sh*ih*priwkcllnvsnvnielv*llrvnllatptqlisfihsskkkvniysmsvnlys
viynkvvlllqlivsfqlv**iiiinswktisknmvifk*mvvlvslmvvfitlqckk*s
kkclinldvlvpnggwiwlkplkislfs
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI548 (VHI548Q) /CSM/VH/VHI5-B/VHI548Q.Seq.d/                       1225   0.0
VHO736 (VHO736Q) /CSM/VH/VHO7-B/VHO736Q.Seq.d/                       1223   0.0
VHI735 (VHI735Q) /CSM/VH/VHI7-B/VHI735Q.Seq.d/                       1223   0.0
VHC779 (VHC779Q) /CSM/VH/VHC7-D/VHC779Q.Seq.d/                       1223   0.0
VHB407 (VHB407Q) /CSM/VH/VHB4-A/VHB407Q.Seq.d/                       1223   0.0
SHA235 (SHA235Q) /CSM/SH/SHA2-B/SHA235Q.Seq.d/                       1223   0.0
CHL203 (CHL203Q) /CSM/CH/CHL2-A/CHL203Q.Seq.d/                       1223   0.0
AHO632 (AHO632Q) /CSM/AH/AHO6-B/AHO632Q.Seq.d/                       1223   0.0
AHH678 (AHH678Q) /CSM/AH/AHH6-D/AHH678Q.Seq.d/                       1223   0.0
VHA389 (VHA389Q) /CSM/VH/VHA3-D/VHA389Q.Seq.d/                       1217   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441982) Dictyostelium discoideum cDNA clone:ddv48o11, 3' ...  1225   0.0    1
(X94340) D.discoideum mRNA for 6-phosphofructo-1-kinase.         1223   0.0    1
(X89039) D.discoideum mRNA for 6-phosphofructo-1-kinase.         1223   0.0    1
(AC116957) Dictyostelium discoideum chromosome 2 map 1685067...  1223   0.0    1
(BJ445699) Dictyostelium discoideum cDNA clone:ddv60h09, 3' ...  1223   0.0    1
(BJ441935) Dictyostelium discoideum cDNA clone:ddv48f09, 3' ...  1223   0.0    1
(BJ438274) Dictyostelium discoideum cDNA clone:ddv36n19, 3' ...  1223   0.0    1
(BJ437095) Dictyostelium discoideum cDNA clone:ddv33n02, 3' ...  1223   0.0    1
(BJ403174) Dictyostelium discoideum cDNA clone:dds24e10, 3' ...  1223   0.0    1
(BJ382228) Dictyostelium discoideum cDNA clone:ddc44e02, 3' ...  1223   0.0    1
dna update 2008.11.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(P90521) RecName: Full=6-phosphofructokinase;          Short=Pho...   414   e-114
CP001337_2617(CP001337|pid:none) Chloroflexus aggregans DSM 9485...   186   9e-46
AE017283_2204(AE017283|pid:none) Propionibacterium acnes KPA1712...   161   2e-38
BC081652_1(BC081652|pid:none) Danio rerio phosphofructokinase, m...   160   4e-38
(Q867C9) RecName: Full=6-phosphofructokinase, muscle type;      ...   153   5e-36
BC057710_1(BC057710|pid:none) Xenopus laevis hypothetical protei...   153   5e-36
AY335614_1(AY335614|pid:none) Synthetic construct Homo sapiens p...   152   1e-35
CR858939_1(CR858939|pid:none) Pongo abelii mRNA; cDNA DKFZp468O1...   152   1e-35
DQ480147_1(DQ480147|pid:none) Sus scrofa muscle 6-phosphofructok...   152   1e-35
(P08237) RecName: Full=6-phosphofructokinase, muscle type;      ...   152   1e-35
protein update 2009. 7.13
PSORT 

 psg: 0.03  gvh: 0.31  alm: 0.36  top: 0.53  tms: 0.00  mit: 0.27  mip: 0.00
 nuc: 0.48  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 76.0 %: nuclear
	 12.0 %: cytoskeletal
	 12.0 %: cytoplasmic

>> prediction for VHI548 is nuc
5' end seq. ID VHI548F
5' end seq. 
>VHI548F.Seq
AATTTAAACAATATATAAATCAATTAAAATAAACACTCACTCGATAGTGTAAAAGAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTNAAAAAAAAAN
NNNNNNNNN
Length of 5' end seq. 129
3' end seq. ID VHI548Z
3' end seq. 
>VHI548Z.Seq
NNNNNNNNNNAAGTGATACCTCTTTGAATAACACATTGGATGCATTAGATAAAATCAAAC
AATCAGCAGTGGCATCACGTCGTTTATTCGTCGTGGAGGTCATGGGTGCCCATTGTGGTT
ATTTGGCTGCAATGAGTTCTTTAACCTCAGGTGCTGAACGTTCATATATAATGGAAAGAG
GTATCACATTGAATACACTAACCAAGGATTTGGAAATGTTTGTTGAACGTTTCAAACGTG
AACATAGAATTGGTCTAATTATTAAGAGTGAACTTGCTAGCAACACCTACTCAACTCATT
TCATTTATTCACTCTTCAAAGAAGAAGGTAAACATTTATTCGATGTCCGTGAATCTATAC
TCGGTCATTTACAACAAGGTGGTACTCCTTCTGCAATTGATCGTATCTTTTCAACTCGTT
TAATGAATCATTATTATCAATTCTTGGAAAACGATCTCAAAGAACATGGTCATCTTCAAA
TGAATGGTTGTATTGGTTTCATTGATGGTGGTATTCATTACACTCCAATGCAAGAAATGA
TCGAAGAAATGTCTGATAAATTTAGACGTCCTCGTTCCCAATGGTGGATGGATTTGGTTG
AAACCTCTCAAAATATCTCTGTTTTCCT
Length of 3' end seq. 628
Connected seq. ID VHI548P
Connected seq. 
>VHI548P.Seq
AATTTAAACAATATATAAATCAATTAAAATAAACACTCACTCGATAGTGTAAAAGAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAANNTTTTTTNAAAAAAAAA-
---------AAGTGATACCTCTTTGAATAACACATTGGATGCATTAGATAAAATCAAACA
ATCAGCAGTGGCATCACGTCGTTTATTCGTCGTGGAGGTCATGGGTGCCCATTGTGGTTA
TTTGGCTGCAATGAGTTCTTTAACCTCAGGTGCTGAACGTTCATATATAATGGAAAGAGG
TATCACATTGAATACACTAACCAAGGATTTGGAAATGTTTGTTGAACGTTTCAAACGTGA
ACATAGAATTGGTCTAATTATTAAGAGTGAACTTGCTAGCAACACCTACTCAACTCATTT
CATTTATTCACTCTTCAAAGAAGAAGGTAAACATTTATTCGATGTCCGTGAATCTATACT
CGGTCATTTACAACAAGGTGGTACTCCTTCTGCAATTGATCGTATCTTTTCAACTCGTTT
AATGAATCATTATTATCAATTCTTGGAAAACGATCTCAAAGAACATGGTCATCTTCAAAT
GAATGGTTGTATTGGTTTCATTGATGGTGGTATTCATTACACTCCAATGCAAGAAATGAT
CGAAGAAATGTCTGATAAATTTAGACGTCCTCGTTCCCAATGGTGGATGGATTTGGTTGA
AACCTCTCAAAATATCTCTGTTTTCCT
Length of connected seq. 737
Full length Seq ID -
Full length Seq. -
Length of full length seq. -