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Dicty_cDB
Contig List | Clone List | cDNA Library List
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI547
Library VH
(Link to library)
Clone ID VHI547
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U16423-1
Original site URL
Representative seq. ID VHI547P
(Link to Original site)
Representative DNA sequence 
>VHI547 (VHI547Q) /CSM/VH/VHI5-B/VHI547Q.Seq.d/
AATTTTNTCCCGCGGATAAATTTTTTTAAAGAATTAGTCATTTTATGCCAATTGGAAATA
ACAAAGATAACGATGGAACTACCCTGAATATCTCAGGGTTAACCATCTCTGATAAGAGTT
CGTCAACTTCATCAAACAACAACACAACAACAACAACAACACAAACTAGTGAACCATATG
TCCCACCATCTCGTAGAAACAAGATGAATAATAGTTCAGGTTCATCAAGAGACGATTATC
CATCACCATCTGACCGTGATAATCGTGATTCACCATTCAATCGTGACCGTCGTGACGATG
GTTACAGAGGTGGTAACAATTTTAGGGAAAATAACTCTAATAACAACAACAACAGTAGAG
ATAACTACAAAAGTCCATCATTCTCAAGAAACAATAATAGTAATGGTGGTGGTAGTAGTA
GTGGTAGCGGTGGTTGGGATAATGGTCCAAGAGAACAAGGCCCACCAAGAGGTAATAGTT
ACTCTTATTCACCAAATAATTCATTCTCTCGTGGTGGTAATCAAGGTGGTTATGGTAATA
GAGGCGGAAGTGGCAGTAGTTTTGGTAAACCAGCCAATAAAAATGATCGTXXXXXXXXXX
TTTGTCTCATTTTCGTTGAAACTAAACGTTCTTGTGATACTCTTACCTACTTTTTAAATC
AAAGAAATTTCCCAACTACCTGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAATG
CACTTCAATCTTTCCGTTCTTTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCAA
GAGGTCTCCACATCGGTAATGTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATAC
ATATCTACGTTCATCGTATTGGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATTT
CCTTCTTCAATGAAAAGAATAAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCTT
CAAATCAAGATATTCCAGATTGGTTTGAAAAGATGGTACACAATCTCCGTATGTCTAAAG
GTCCATCAAACTCAAAATCAAATAGTCCATTCAACAAATCTTACAATAGTCATCACAATA
GAGATGATAACCGTGGTGGTTATGGTGGTGGTGGTGGTGGTAATGGAGGTTACAGTAACA
ACCGTAATGATAGAAGAGAAGATAAATCAGATTACTCAATGCATCACCCATACTTTAGCA
ACAATGGTGGTTCATATAATTAAACATTCACCTCATGTCATTCGTTTAATAAAAAATAAT
AATCCATTTNATTCAATA
sequence update 2002.10.25
Translated Amino Acid sequence 
fxpadkff*RISHFMPIGNNKDNDGTTLNISGLTISDKSSSTSSNNNTTTTTTQTSEPYV
PPSRRNKMNNSSGSSRDDYPSPSDRDNRDSPFNRDRRDDGYRGGNNFRENNSNNNNNSRD
NYKSPSFSRNNNSNGGGSSSGSGGWDNGPREQGPPRGNSYSYSPNNSFSRGGNQGGYGNR
GGSGSSFGKPANKNDR---

---CLIFVETKRSCDTLTYFLNQRNFPTTCIHGDLTQPERENALQSFRSFATPFLVATDI
ASRGLHIGNVNLVINFDLPTDIHIYVHRIGRTGRAGKKGLAISFFNEKNKPVGAELLKLM
KASNQDIPDWFEKMVHNLRMSKGPSNSKSNSPFNKSYNSHHNRDDNRGGYGGGGGGNGGY
SNNRNDRREDKSDYSMHHPYFSNNGGSYN*tftschsfnkk**sixfn
Translated Amino Acid sequence (All Frames) 
Frame A:
nfxprinffkelvilcqleitkitmelp*isqg*pslirvrqlhqtttqqqqqhklvnhm
shhlvetr*iivqvhqetiihhhltviivihhsivtvvtmvtevvtilgkitlittttve
ittkvhhsqetiivmvvvvvvvavvgimvqenkahqevivtlihqiihslvvvikvvmvi
eaevavvlvnqpikmi---

---fvsfslklnvlvillptf*ikeisqlpvfmvi*lnqkekmhfnlsvllqlhs**qli
lhqevstsvmliwlsistclptyistfivlvepeeqekkv*qfpssmkrinqlvlnf*n*
*klqikifqiglkrwytisvclkvhqtqnqivhstnltivitiemitvvvmvvvvvvmev
tvttvmieekinqitqcithtlatmvvhiikhsphvirliknnnpfxsi

Frame B:
ixsrg*iflkn*sfyanwk*qr*rwnypeylrvnhl**efvnfikqqhnnnnntn**tic
ptis*kqde**frfikrrlsiti*p**s*ftiqs*ps*rwlqrw*qf*gk*l**qqqq*r
*lqksiilkkq***www***w*rwlg*wskrtrptkr**lllftk*filsww*srwlw**
rrkwq*fw*tsq*k*s---

---lshfr*n*tfl*ysyllfkskkfpnylysw*fnstrkrkctsifpffcnsilssn*y
cikrsphr*c*fgyqfrlayrhtylrssyw*nrksrkkrfsnfllq*ke*tswc*tfein
esfksrysrlv*kdgtqspyv*rsiklkik*siqqilq*ssq*r**pwwlwwwwww*wrl
q*qp***krr*irllnaspil*qqwwfi*lnihlmsfv**kiiihxiq

Frame C:
fxpadkff*RISHFMPIGNNKDNDGTTLNISGLTISDKSSSTSSNNNTTTTTTQTSEPYV
PPSRRNKMNNSSGSSRDDYPSPSDRDNRDSPFNRDRRDDGYRGGNNFRENNSNNNNNSRD
NYKSPSFSRNNNSNGGGSSSGSGGWDNGPREQGPPRGNSYSYSPNNSFSRGGNQGGYGNR
GGSGSSFGKPANKNDR---

---CLIFVETKRSCDTLTYFLNQRNFPTTCIHGDLTQPERENALQSFRSFATPFLVATDI
ASRGLHIGNVNLVINFDLPTDIHIYVHRIGRTGRAGKKGLAISFFNEKNKPVGAELLKLM
KASNQDIPDWFEKMVHNLRMSKGPSNSKSNSPFNKSYNSHHNRDDNRGGYGGGGGGNGGY
SNNRNDRREDKSDYSMHHPYFSNNGGSYN*tftschsfnkk**sixfn
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI547 (VHI547Q) /CSM/VH/VHI5-B/VHI547Q.Seq.d/                       1735   0.0
VHQ404 (VHQ404Q) /CSM/VH/VHQ4-A/VHQ404Q.Seq.d/                        934   0.0
VHP588 (VHP588Q) /CSM/VH/VHP5-D/VHP588Q.Seq.d/                        934   0.0
VHP118 (VHP118Q) /CSM/VH/VHP1-A/VHP118Q.Seq.d/                        934   0.0
VHI263 (VHI263Q) /CSM/VH/VHI2-C/VHI263Q.Seq.d/                        934   0.0
CHL756 (CHL756Q) /CSM/CH/CHL7-C/CHL756Q.Seq.d/                        934   0.0
CHH489 (CHH489Q) /CSM/CH/CHH4-D/CHH489Q.Seq.d/                        934   0.0
CHF867 (CHF867Q) /CSM/CH/CHF8-C/CHF867Q.Seq.d/                        934   0.0
CHF591 (CHF591Q) /CSM/CH/CHF5-D/CHF591Q.Seq.d/                        934   0.0
AFC476 (AFC476Q) /CSM/AF/AFC4-D/AFC476Q.Seq.d/                        934   0.0
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ423208) Dictyostelium discoideum cDNA clone:ddv48m11, 5' ...   841   0.0    3
(BJ419288) Dictyostelium discoideum cDNA clone:ddv35j16, 5' ...   841   0.0    3
(BJ358087) Dictyostelium discoideum cDNA clone:dda65p14, 3' ...   726   0.0    5
(BJ446556) Dictyostelium discoideum cDNA clone:ddv63h02, 3' ...   555   0.0    3
(BJ446510) Dictyostelium discoideum cDNA clone:ddv62o21, 3' ...   555   0.0    3
(BJ445908) Dictyostelium discoideum cDNA clone:ddv61c05, 3' ...   555   0.0    3
(BJ441972) Dictyostelium discoideum cDNA clone:ddv48m11, 3' ...   555   0.0    3
(BJ441733) Dictyostelium discoideum cDNA clone:ddv47m16, 3' ...   555   0.0    3
(BJ385892) Dictyostelium discoideum cDNA clone:ddc56a18, 3' ...   555   0.0    3
(BJ382734) Dictyostelium discoideum cDNA clone:ddc45p13, 3' ...   555   0.0    3
dna update 2008.11.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

(Q54QS3) RecName: Full=Probable ATP-dependent RNA helicase ddx3;...   319   2e-85
(Q6Z4K6) RecName: Full=DEAD-box ATP-dependent RNA helicase 52B; ...   161   4e-38
BT085991_1(BT085991|pid:none) Zea mays full-length cDNA clone ZM...   157   6e-37
(Q75HJ0) RecName: Full=DEAD-box ATP-dependent RNA helicase 37;  ...   157   8e-37
FN357514_12(FN357514|pid:none) Schistosoma mansoni genome sequen...   157   8e-37
EU953399_1(EU953399|pid:none) Zea mays clone 1406878 unknown mRNA.    157   1e-36
(Q2R1M8) RecName: Full=DEAD-box ATP-dependent RNA helicase 52C; ...   155   4e-36
AY062446_1(AY062446|pid:none) Arabidopsis thaliana ATP-dependent...   154   5e-36
AM048814_1(AM048814|pid:none) Platynereis dumerilii mRNA for PL1...   154   7e-36
AM444101_1(AM444101|pid:none) Vitis vinifera contig VV78X103110....   154   9e-36
protein update 2009. 7.13
PSORT 

 psg: 0.88  gvh: 0.39  alm: 0.46  top: 0.30  tms: 0.00  mit: 0.22  mip: 0.00
 nuc: 0.11  erl: 0.00  erm: 0.00  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 48.0 %: cytoplasmic
	 44.0 %: nuclear
	  4.0 %: vesicles of secretory system
	  4.0 %: endoplasmic reticulum

>> prediction for VHI547 is cyt
5' end seq. ID VHI547F
5' end seq. 
>VHI547F.Seq
AATTTTNTCCCGCGGATAAATTTTTTTAAAGAATTAGTCATTTTATGCCAATTGGAAATA
ACAAAGATAACGATGGAACTACCCTGAATATCTCAGGGTTAACCATCTCTGATAAGAGTT
CGTCAACTTCATCAAACAACAACACAACAACAACAACAACACAAACTAGTGAACCATATG
TCCCACCATCTCGTAGAAACAAGATGAATAATAGTTCAGGTTCATCAAGAGACGATTATC
CATCACCATCTGACCGTGATAATCGTGATTCACCATTCAATCGTGACCGTCGTGACGATG
GTTACAGAGGTGGTAACAATTTTAGGGAAAATAACTCTAATAACAACAACAACAGTAGAG
ATAACTACAAAAGTCCATCATTCTCAAGAAACAATAATAGTAATGGTGGTGGTAGTAGTA
GTGGTAGCGGTGGTTGGGATAATGGTCCAAGAGAACAAGGCCCACCAAGAGGTAATAGTT
ACTCTTATTCACCAAATAATTCATTCTCTCGTGGTGGTAATCAAGGTGGTTATGGTAATA
GAGGCGGAAGTGGCAGTAGTTTTGGTAAACCAGCCAATAAAAATGATCGTNNNNNNNNNN
Length of 5' end seq. 600
3' end seq. ID VHI547Z
3' end seq. 
>VHI547Z.Seq
NNNNNNNNNNTTTGTCTCATTTTCGTTGAAACTAAACGTTCTTGTGATACTCTTACCTAC
TTTTTAAATCAAAGAAATTTCCCAACTACCTGTATTCATGGTGATTTAACTCAACCAGAA
AGAGAAAATGCACTTCAATCTTTCCGTTCTTTTGCAACTCCATTCTTAGTAGCAACTGAT
ATTGCATCAAGAGGTCTCCACATCGGTAATGTTAATTTGGTTATCAATTTCGACTTGCCT
ACCGACATACATATCTACGTTCATCGTATTGGTAGAACCGGAAGAGCAGGAAAAAAAGGT
TTAGCAATTTCCTTCTTCAATGAAAAGAATAAACCAGTTGGTGCTGAACTTTTGAAATTA
ATGAAAGCTTCAAATCAAGATATTCCAGATTGGTTTGAAAAGATGGTACACAATCTCCGT
ATGTCTAAAGGTCCATCAAACTCAAAATCAAATAGTCCATTCAACAAATCTTACAATAGT
CATCACAATAGAGATGATAACCGTGGTGGTTATGGTGGTGGTGGTGGTGGTAATGGAGGT
TACAGTAACAACCGTAATGATAGAAGAGAAGATAAATCAGATTACTCAATGCATCACCCA
TACTTTAGCAACAATGGTGGTTCATATAATTAAACATTCACCTCATGTCATTCGTTTAAT
AAAAAATAATAATCCATTTNATTCAATA
Length of 3' end seq. 688
Connected seq. ID VHI547P
Connected seq. 
>VHI547P.Seq
AATTTTNTCCCGCGGATAAATTTTTTTAAAGAATTAGTCATTTTATGCCAATTGGAAATA
ACAAAGATAACGATGGAACTACCCTGAATATCTCAGGGTTAACCATCTCTGATAAGAGTT
CGTCAACTTCATCAAACAACAACACAACAACAACAACAACACAAACTAGTGAACCATATG
TCCCACCATCTCGTAGAAACAAGATGAATAATAGTTCAGGTTCATCAAGAGACGATTATC
CATCACCATCTGACCGTGATAATCGTGATTCACCATTCAATCGTGACCGTCGTGACGATG
GTTACAGAGGTGGTAACAATTTTAGGGAAAATAACTCTAATAACAACAACAACAGTAGAG
ATAACTACAAAAGTCCATCATTCTCAAGAAACAATAATAGTAATGGTGGTGGTAGTAGTA
GTGGTAGCGGTGGTTGGGATAATGGTCCAAGAGAACAAGGCCCACCAAGAGGTAATAGTT
ACTCTTATTCACCAAATAATTCATTCTCTCGTGGTGGTAATCAAGGTGGTTATGGTAATA
GAGGCGGAAGTGGCAGTAGTTTTGGTAAACCAGCCAATAAAAATGATCGT----------
TTTGTCTCATTTTCGTTGAAACTAAACGTTCTTGTGATACTCTTACCTACTTTTTAAATC
AAAGAAATTTCCCAACTACCTGTATTCATGGTGATTTAACTCAACCAGAAAGAGAAAATG
CACTTCAATCTTTCCGTTCTTTTGCAACTCCATTCTTAGTAGCAACTGATATTGCATCAA
GAGGTCTCCACATCGGTAATGTTAATTTGGTTATCAATTTCGACTTGCCTACCGACATAC
ATATCTACGTTCATCGTATTGGTAGAACCGGAAGAGCAGGAAAAAAAGGTTTAGCAATTT
CCTTCTTCAATGAAAAGAATAAACCAGTTGGTGCTGAACTTTTGAAATTAATGAAAGCTT
CAAATCAAGATATTCCAGATTGGTTTGAAAAGATGGTACACAATCTCCGTATGTCTAAAG
GTCCATCAAACTCAAAATCAAATAGTCCATTCAACAAATCTTACAATAGTCATCACAATA
GAGATGATAACCGTGGTGGTTATGGTGGTGGTGGTGGTGGTAATGGAGGTTACAGTAACA
ACCGTAATGATAGAAGAGAAGATAAATCAGATTACTCAATGCATCACCCATACTTTAGCA
ACAATGGTGGTTCATATAATTAAACATTCACCTCATGTCATTCGTTTAATAAAAAATAAT
AATCCATTTNATTCAATA
Length of connected seq. 1268
Full length Seq ID -
Full length Seq. -
Length of full length seq. -