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Dicty_cDB
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NBDC - LSDB Archive
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Dicty_cDB
Contig List | Clone List | cDNA Library List
Atlas
Atlas | Atlas Image List
VHI545
Library VH
(Link to library)
Clone ID VHI545
Atlas ID -
NBRP ID -
dictyBase ID -
Link to Contig Contig-U15918-1
Original site URL
Representative seq. ID VHI545P
(Link to Original site)
Representative DNA sequence 
>VHI545 (VHI545Q) /CSM/VH/VHI5-B/VHI545Q.Seq.d/
GTGAAATCGCAAACCACAAAAGGGGATCACAGTTATAGAAAAACGATAAAGAGTGAGACA
GGAGAGAGAGTAGTAAGAGCGCACATAGTATTAATAAATAATAAATAACTAATAATAAAA
TAATAAATAACTAATAATAAAATAATAATAATATTTTAAATAGTAATAATTTCTACATAT
ATTTGAAAAATAAAAAAAGAATTAATTTTATAATAATAATATTAATAATAACAACAATAA
TAACAATTTTAAAATAATACTATATTTTAAATAAATACATACAAATAAACATACACACAC
ACACACACACACACACATATATATATATATAATAAATTTTTCTATTTTTAAATAATAAAT
AATAATAATTAATAATTAATAATAATAAAAAGAANTGAGTGCCCCAAATAATAATAATAA
TAATACTACTACTACAACTACAACATCACCATCACCATCACANAGCTCACCAACATTAAC
GTNAGTAGCATCAAATAGTACAACAACAACAACAACAGAAACATTAACTACACCAAAATT
ACAATNTGTAGTTAGATCAAAAACCAGTGCATATCAAGAGXXXXXXXXXXTAATATGCCA
TCACAAACTACACTTTTCAATTTAAAGGGTGAACCATTGGTCGATTTTGGTCTCAATCCA
AGAAACACCATTCGTTTCTCTCCAAATGGTCAATTGTTATGTTTGGGTGGTTTTGGTAAT
CTTCAAGGTGATATGGACTTTTGGGATTTAACACGTTACAAGAGAATTTGTGGCACTCAA
TCTCATTGTGCCATTTACACTGAATGGTCTGCTGATTCCGTTCACTTTATGACCGCTGTC
CTTTCACCACGTATTCGTGTTGACAATGGTGTTAAAATCATCAAATACGATAACACTATC
GTCTATCAAGAAAACATACCTGAACTTTATCAAGCCTCTTGGAGACCTTTAAATCCACTC
GTCTTCCCAAATGAAAGAATCGTCTATCCAAGTATACAACAACAAAAAGAATCTTCACCA
CAACCACAAAAATATACACCACCATCACTTAGAAATATGCAAGCTGCTCCACCAGTTGTA
ACTTCACCACCTGCAATGGGTGCTCCACTTCCAAGTGGTTTTAAAGTTTATTTAGCTTCT
GCCAAATCATCATCAACTTTTAAACCAAAACAAAAACCATCATCAACAACAACAACAAAC
AATACTACCACCACCACAACAAAACCAGCTGCAGATGAACCAAAGAGAGAACTAACACCA
ATGAAAAGAAAATTAGAAATGTGAAAGAAAACTCAAAG
sequence update 2002.10.25
Translated Amino Acid sequence 
*nrkpqkgitviekr*rvrqere**ert*y**iinn***nnk*liik***yfk***flhi
fek*kkn*fynnninnnnnnnnfkiilyfk*ihtnkhththththiyiyiinfsifk**i
iiinn****KEXSAPNNNNNNTTTTTTTSPSPSXSSPTLTXVASNSTTTTTTETLTTPKL
QXVVRSKTSAYQE---

---NMPSQTTLFNLKGEPLVDFGLNPRNTIRFSPNGQLLCLGGFGNLQGDMDFWDLTRYK
RICGTQSHCAIYTEWSADSVHFMTAVLSPRIRVDNGVKIIKYDNTIVYQENIPELYQASW
RPLNPLVFPNERIVYPSIQQQKESSPQPQKYTPPSLRNMQAAPPVVTSPPAMGAPLPSGF
KVYLASAKSSSTFKPKQKPSSTTTTNNTTTTTTKPAADEPKRELTPMKRKLEM*kktq
Translated Amino Acid sequence (All Frames) 
Frame A:
vksqttkgdhsyrktiksetgervvrahivlinnk*liik**itnnkiiiif*iviisty
i*kikkelil***y***qq**qf*nntif*intyk*tyththththiyiynkffyf*iin
nnn**liiikrxecpk*****yyyynynitititxltninvssik*ynnnnnrninytki
txcs*iknqcisr---

---*yaitnytfqfkg*tigrfwsqskkhhsflskwsivmfgwfw*ssr*ygllgfntlq
enlwhsislchlh*mvc*frslydrcpfttysc*qwc*nhqir*hyrlsrkht*tlssll
etfkstrlpk*knrlskytttkrifttttkiyttit*kyascstscnfttcngcstskwf
*slfsfcqiiinf*tktktiinnnnkqyyhhhnktscr*tkertntnekkirnvkensk

Frame B:
*nrkpqkgitviekr*rvrqere**ert*y**iinn***nnk*liik***yfk***flhi
fek*kkn*fynnninnnnnnnnfkiilyfk*ihtnkhththththiyiyiinfsifk**i
iiinn****KEXSAPNNNNNNTTTTTTTSPSPSXSSPTLTXVASNSTTTTTTETLTTPKL
QXVVRSKTSAYQE---

---NMPSQTTLFNLKGEPLVDFGLNPRNTIRFSPNGQLLCLGGFGNLQGDMDFWDLTRYK
RICGTQSHCAIYTEWSADSVHFMTAVLSPRIRVDNGVKIIKYDNTIVYQENIPELYQASW
RPLNPLVFPNERIVYPSIQQQKESSPQPQKYTPPSLRNMQAAPPVVTSPPAMGAPLPSGF
KVYLASAKSSSTFKPKQKPSSTTTTNNTTTTTTKPAADEPKRELTPMKRKLEM*kktq

Frame C:
eianhkrgsql*kndke*drressksahsink**itnnkiinn***nnnnilnsnnfyiy
lknkkrinfiiiiliittiitilk*yyilnkyiqinihthththtyiyi**iflflnnk*
**liinnnkkx*vpqiiiiiilllqlqhhhhhhxahqh*rx*hqivqqqqqqkh*lhqny
nx*ldqkpvhik---

---ichhklhfsi*rvnhwsilvsiqetpfvslqmvncyvwvvlvifkviwtfgi*hvtr
efvalnlivpftlngllipftl*plsfhhvfvltmvlkssntitlssikktylnfikplg
dl*ihsssqmkessiqvynnkknlhhnhknihhhhleickllhql*lhhlqwvlhfqvvl
kfi*llpnhhqllnqnknhhqqqqqtilpppqqnqlqmnqren*hq*ken*kcerklk
Homology vs CSM-cDNA 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

VHI545 (VHI545Q) /CSM/VH/VHI5-B/VHI545Q.Seq.d/                       1211   0.0
CHQ221 (CHQ221Q) /CSM/CH/CHQ2-A/CHQ221Q.Seq.d/                        878   0.0
CHN492 (CHN492Q) /CSM/CH/CHN4-D/CHN492Q.Seq.d/                        844   0.0
VHO528 (VHO528Q) /CSM/VH/VHO5-B/VHO528Q.Seq.d/                        757   0.0
FC-AJ01 (FC-AJ01Q) /CSM/FC/FC-AJ/FC-AJ01Q.Seq.d/                      755   0.0
VHG503 (VHG503Q) /CSM/VH/VHG5-A/VHG503Q.Seq.d/                        593   e-168
VHG827 (VHG827Q) /CSM/VH/VHG8-B/VHG827Q.Seq.d/                        561   e-159
VSB134 (VSB134Q) /CSM/VS/VSB1-B/VSB134Q.Seq.d/                        496   e-139
CHJ460 (CHJ460Q) /CSM/CH/CHJ4-C/CHJ460Q.Seq.d/                        232   1e-59
SLK393 (SLK393Q) /CSM/SL/SLK3-D/SLK393Q.Seq.d/                        153   8e-36
own update 2004.12.24
Homology vs DNA 

                                                             Score    E
Sequences producing significant alignments:                  (bits) Value  N

(BJ441953) Dictyostelium discoideum cDNA clone:ddv48i11, 3' ...  1094   0.0    1
(BJ385184) Dictyostelium discoideum cDNA clone:ddc54i06, 3' ...   841   0.0    3
(BJ384269) Dictyostelium discoideum cDNA clone:ddc48h24, 3' ...   807   0.0    3
(BJ445693) Dictyostelium discoideum cDNA clone:ddv60g07, 3' ...   720   0.0    4
(C23783) Dictyostelium discoideum gamete cDNA, clone FC-AJ01.     690   0.0    5
(BJ440550) Dictyostelium discoideum cDNA clone:ddv44e01, 3' ...   555   e-174  4
(BJ440640) Dictyostelium discoideum cDNA clone:ddv44f08, 3' ...   523   e-165  4
(AU261838) Dictyostelium discoideum vegetative cDNA clone:VS...   458   e-145  4
(BJ423190) Dictyostelium discoideum cDNA clone:ddv48i11, 5' ...   180   e-101  3
(BJ426807) Dictyostelium discoideum cDNA clone:ddv60g07, 5' ...   180   4e-97  3
dna update 2008.11.13
Homology vs Protein 

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AC165439_15(AC165439|pid:none) Medicago truncatula clone mth2-14...   135   4e-30
BC161516_1(BC161516|pid:none) Xenopus tropicalis hypothetical pr...   131   6e-29
(Q7ZY11) RecName: Full=Eukaryotic translation initiation factor ...   130   8e-29
BC044026_1(BC044026|pid:none) Xenopus laevis Eukaryotic translat...   130   8e-29
AF172818_1(AF172818|pid:none) Homo sapiens MSTP089 (MST089) mRNA...   129   3e-28
AF212241_1(AF212241|pid:none) Homo sapiens CDA02 mRNA, complete ...   129   3e-28
BC011885_1(BC011885|pid:none) Homo sapiens eukaryotic translatio...   129   3e-28
AF497978_1(AF497978|pid:none) Homo sapiens eukaryotic translatio...   129   3e-28
BT060641_1(BT060641|pid:none) Zea mays full-length cDNA clone ZM...   128   4e-28
(Q8BJW6) RecName: Full=Eukaryotic translation initiation factor ...   127   9e-28
protein update 2009. 7.13
PSORT 

 psg: 0.85  gvh: 0.48  alm: 0.48  top: 0.60  tms: 0.00  mit: 0.49  mip: 0.10
 nuc: 0.06  erl: 0.00  erm: 0.20  pox: 0.00  px2: 0.00  vac: 0.00  rnp: 0.00
 act: 0.00  caa: 0.00  yqr: 0.00  tyr: 0.00  leu: 0.00  gpi: 0.00  myr: 0.00
 dna: 0.00  rib: 0.00  bac: 0.00  m1a: 0.00  m1b: 0.00  m2 : 0.00  mNt: 0.00
 m3a: 0.00  m3b: 0.00  m_ : 1.00 

	 64.0 %: mitochondrial
	 16.0 %: nuclear
	  8.0 %: cytoplasmic
	  4.0 %: cytoskeletal
	  4.0 %: extracellular, including cell wall
	  4.0 %: peroxisomal

>> prediction for VHI545 is mit
5' end seq. ID VHI545F
5' end seq. 
>VHI545F.Seq
GTGAAATCGCAAACCACAAAAGGGGATCACAGTTATAGAAAAACGATAAAGAGTGAGACA
GGAGAGAGAGTAGTAAGAGCGCACATAGTATTAATAAATAATAAATAACTAATAATAAAA
TAATAAATAACTAATAATAAAATAATAATAATATTTTAAATAGTAATAATTTCTACATAT
ATTTGAAAAATAAAAAAAGAATTAATTTTATAATAATAATATTAATAATAACAACAATAA
TAACAATTTTAAAATAATACTATATTTTAAATAAATACATACAAATAAACATACACACAC
ACACACACACACACACATATATATATATATAATAAATTTTTCTATTTTTAAATAATAAAT
AATAATAATTAATAATTAATAATAATAAAAAGAANTGAGTGCCCCAAATAATAATAATAA
TAATACTACTACTACAACTACAACATCACCATCACCATCACANAGCTCACCAACATTAAC
GTNAGTAGCATCAAATAGTACAACAACAACAACAACAGAAACATTAACTACACCAAAATT
ACAATNTGTAGTTAGATCAAAAACCAGTGCATATCAAGAGNNNNNNNNNN
Length of 5' end seq. 590
3' end seq. ID VHI545Z
3' end seq. 
>VHI545Z.Seq
NNNNNNNNNNTAATATGCCATCACAAACTACACTTTTCAATTTAAAGGGTGAACCATTGG
TCGATTTTGGTCTCAATCCAAGAAACACCATTCGTTTCTCTCCAAATGGTCAATTGTTAT
GTTTGGGTGGTTTTGGTAATCTTCAAGGTGATATGGACTTTTGGGATTTAACACGTTACA
AGAGAATTTGTGGCACTCAATCTCATTGTGCCATTTACACTGAATGGTCTGCTGATTCCG
TTCACTTTATGACCGCTGTCCTTTCACCACGTATTCGTGTTGACAATGGTGTTAAAATCA
TCAAATACGATAACACTATCGTCTATCAAGAAAACATACCTGAACTTTATCAAGCCTCTT
GGAGACCTTTAAATCCACTCGTCTTCCCAAATGAAAGAATCGTCTATCCAAGTATACAAC
AACAAAAAGAATCTTCACCACAACCACAAAAATATACACCACCATCACTTAGAAATATGC
AAGCTGCTCCACCAGTTGTAACTTCACCACCTGCAATGGGTGCTCCACTTCCAAGTGGTT
TTAAAGTTTATTTAGCTTCTGCCAAATCATCATCAACTTTTAAACCAAAACAAAAACCAT
CATCAACAACAACAACAAACAATACTACCACCACCACAACAAAACCAGCTGCAGATGAAC
CAAAGAGAGAACTAACACCAATGAAAAGAAAATTAGAAATGTGAAAGAAAACTCAAAG
Length of 3' end seq. 718
Connected seq. ID VHI545P
Connected seq. 
>VHI545P.Seq
GTGAAATCGCAAACCACAAAAGGGGATCACAGTTATAGAAAAACGATAAAGAGTGAGACA
GGAGAGAGAGTAGTAAGAGCGCACATAGTATTAATAAATAATAAATAACTAATAATAAAA
TAATAAATAACTAATAATAAAATAATAATAATATTTTAAATAGTAATAATTTCTACATAT
ATTTGAAAAATAAAAAAAGAATTAATTTTATAATAATAATATTAATAATAACAACAATAA
TAACAATTTTAAAATAATACTATATTTTAAATAAATACATACAAATAAACATACACACAC
ACACACACACACACACATATATATATATATAATAAATTTTTCTATTTTTAAATAATAAAT
AATAATAATTAATAATTAATAATAATAAAAAGAANTGAGTGCCCCAAATAATAATAATAA
TAATACTACTACTACAACTACAACATCACCATCACCATCACANAGCTCACCAACATTAAC
GTNAGTAGCATCAAATAGTACAACAACAACAACAACAGAAACATTAACTACACCAAAATT
ACAATNTGTAGTTAGATCAAAAACCAGTGCATATCAAGAG----------TAATATGCCA
TCACAAACTACACTTTTCAATTTAAAGGGTGAACCATTGGTCGATTTTGGTCTCAATCCA
AGAAACACCATTCGTTTCTCTCCAAATGGTCAATTGTTATGTTTGGGTGGTTTTGGTAAT
CTTCAAGGTGATATGGACTTTTGGGATTTAACACGTTACAAGAGAATTTGTGGCACTCAA
TCTCATTGTGCCATTTACACTGAATGGTCTGCTGATTCCGTTCACTTTATGACCGCTGTC
CTTTCACCACGTATTCGTGTTGACAATGGTGTTAAAATCATCAAATACGATAACACTATC
GTCTATCAAGAAAACATACCTGAACTTTATCAAGCCTCTTGGAGACCTTTAAATCCACTC
GTCTTCCCAAATGAAAGAATCGTCTATCCAAGTATACAACAACAAAAAGAATCTTCACCA
CAACCACAAAAATATACACCACCATCACTTAGAAATATGCAAGCTGCTCCACCAGTTGTA
ACTTCACCACCTGCAATGGGTGCTCCACTTCCAAGTGGTTTTAAAGTTTATTTAGCTTCT
GCCAAATCATCATCAACTTTTAAACCAAAACAAAAACCATCATCAACAACAACAACAAAC
AATACTACCACCACCACAACAAAACCAGCTGCAGATGAACCAAAGAGAGAACTAACACCA
ATGAAAAGAAAATTAGAAATGTGAAAGAAAACTCAAAG
Length of connected seq. 1288
Full length Seq ID -
Full length Seq. -
Length of full length seq. -